Hormonal stimulation of carp (

Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Dietrich, Mariola A. [verfasserIn] Irnazarow, Ilgiz [verfasserIn] Inglot, Michał [verfasserIn] Słowińska, Mariola [verfasserIn] Judycka, Sylwia [verfasserIn] Ciereszko, Andrzej [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2020

Schlagwörter:	Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics

Übergeordnetes Werk:	Enthalten in: Aquaculture - Amsterdam [u.a.] : Elsevier Science, 1972, 530
Übergeordnetes Werk:	volume:530

DOI / URN:	10.1016/j.aquaculture.2020.735791

Katalog-ID:	ELV004843983

Internformat


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100	1		\|a Dietrich, Mariola A. \|e verfasserin \|4 aut
245	1	0	\|a Hormonal stimulation of carp (
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520			\|a Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049.
650		4	\|a Fish reproduction
650		4	\|a Hormone
650		4	\|a Maturation
650		4	\|a Spermatozoa
650		4	\|a Spermiation
650		4	\|a Proteomics
700	1		\|a Irnazarow, Ilgiz \|e verfasserin \|4 aut
700	1		\|a Inglot, Michał \|e verfasserin \|4 aut
700	1		\|a Słowińska, Mariola \|e verfasserin \|4 aut
700	1		\|a Judycka, Sylwia \|e verfasserin \|4 aut
700	1		\|a Ciereszko, Andrzej \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Aquaculture \|d Amsterdam [u.a.] : Elsevier Science, 1972 \|g 530 \|h Online-Ressource \|w (DE-627)306314002 \|w (DE-600)1495998-7 \|w (DE-576)094503966 \|7 nnns
773	1	8	\|g volume:530
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912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
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912			\|a GBV_ILN_151
912			\|a GBV_ILN_224
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
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912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
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912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 48.68 \|j Aquakultur
951			\|a AR
952			\|d 530

Indexfelder

author_variant	m a d ma mad i i ii m i mi m s ms s j sj a c ac
matchkey_str	dietrichmariolaairnazarowilgizinglotmich:2020----:omnltmlto
hierarchy_sort_str	2020
bklnumber	48.68
publishDate	2020
allfields	10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530
spelling	10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530
allfields_unstemmed	10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530
allfieldsGer	10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530
allfieldsSound	10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530
language	English
source	Enthalten in Aquaculture 530 volume:530
sourceStr	Enthalten in Aquaculture 530 volume:530
format_phy_str_mv	Article
bklname	Aquakultur
institution	findex.gbv.de
topic_facet	Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics
dewey-raw	570
isfreeaccess_bool	false
container_title	Aquaculture
authorswithroles_txt_mv	Dietrich, Mariola A. @@aut@@ Irnazarow, Ilgiz @@aut@@ Inglot, Michał @@aut@@ Słowińska, Mariola @@aut@@ Judycka, Sylwia @@aut@@ Ciereszko, Andrzej @@aut@@
publishDateDaySort_date	2020-01-01T00:00:00Z
hierarchy_top_id	306314002
dewey-sort	3570
id	ELV004843983
language_de	englisch
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author	Dietrich, Mariola A.
spellingShingle	Dietrich, Mariola A. ddc 570 fid BIODIV bkl 48.68 misc Fish reproduction misc Hormone misc Maturation misc Spermatozoa misc Spermiation misc Proteomics Hormonal stimulation of carp (
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topic_title	570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Hormonal stimulation of carp ( Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics
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title	Hormonal stimulation of carp (
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title_full	Hormonal stimulation of carp (
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title_sort	hormonal stimulation of carp (
title_auth	Hormonal stimulation of carp (
abstract	Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049.
abstractGer	Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049.
abstract_unstemmed	Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049.
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title_short	Hormonal stimulation of carp (
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author2	Irnazarow, Ilgiz Inglot, Michał Słowińska, Mariola Judycka, Sylwia Ciereszko, Andrzej
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up_date	2024-07-07T00:20:09.523Z
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