Hormonal stimulation of carp (
Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation...
Ausführliche Beschreibung
Autor*in: |
Dietrich, Mariola A. [verfasserIn] Irnazarow, Ilgiz [verfasserIn] Inglot, Michał [verfasserIn] Słowińska, Mariola [verfasserIn] Judycka, Sylwia [verfasserIn] Ciereszko, Andrzej [verfasserIn] |
---|
Format: |
E-Artikel |
---|---|
Sprache: |
Englisch |
Erschienen: |
2020 |
---|
Schlagwörter: |
---|
Übergeordnetes Werk: |
Enthalten in: Aquaculture - Amsterdam [u.a.] : Elsevier Science, 1972, 530 |
---|---|
Übergeordnetes Werk: |
volume:530 |
DOI / URN: |
10.1016/j.aquaculture.2020.735791 |
---|
Katalog-ID: |
ELV004843983 |
---|
LEADER | 01000caa a22002652 4500 | ||
---|---|---|---|
001 | ELV004843983 | ||
003 | DE-627 | ||
005 | 20230524125554.0 | ||
007 | cr uuu---uuuuu | ||
008 | 230503s2020 xx |||||o 00| ||eng c | ||
024 | 7 | |a 10.1016/j.aquaculture.2020.735791 |2 doi | |
035 | |a (DE-627)ELV004843983 | ||
035 | |a (ELSEVIER)S0044-8486(20)31153-4 | ||
040 | |a DE-627 |b ger |c DE-627 |e rda | ||
041 | |a eng | ||
082 | 0 | 4 | |a 570 |a 550 |q DE-600 |
084 | |a BIODIV |q DE-30 |2 fid | ||
084 | |a 48.68 |2 bkl | ||
100 | 1 | |a Dietrich, Mariola A. |e verfasserin |4 aut | |
245 | 1 | 0 | |a Hormonal stimulation of carp ( |
264 | 1 | |c 2020 | |
336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
337 | |a Computermedien |b c |2 rdamedia | ||
338 | |a Online-Ressource |b cr |2 rdacarrier | ||
520 | |a Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. | ||
650 | 4 | |a Fish reproduction | |
650 | 4 | |a Hormone | |
650 | 4 | |a Maturation | |
650 | 4 | |a Spermatozoa | |
650 | 4 | |a Spermiation | |
650 | 4 | |a Proteomics | |
700 | 1 | |a Irnazarow, Ilgiz |e verfasserin |4 aut | |
700 | 1 | |a Inglot, Michał |e verfasserin |4 aut | |
700 | 1 | |a Słowińska, Mariola |e verfasserin |4 aut | |
700 | 1 | |a Judycka, Sylwia |e verfasserin |4 aut | |
700 | 1 | |a Ciereszko, Andrzej |e verfasserin |4 aut | |
773 | 0 | 8 | |i Enthalten in |t Aquaculture |d Amsterdam [u.a.] : Elsevier Science, 1972 |g 530 |h Online-Ressource |w (DE-627)306314002 |w (DE-600)1495998-7 |w (DE-576)094503966 |7 nnns |
773 | 1 | 8 | |g volume:530 |
912 | |a GBV_USEFLAG_U | ||
912 | |a SYSFLAG_U | ||
912 | |a GBV_ELV | ||
912 | |a FID-BIODIV | ||
912 | |a SSG-OLC-PHA | ||
912 | |a GBV_ILN_20 | ||
912 | |a GBV_ILN_22 | ||
912 | |a GBV_ILN_23 | ||
912 | |a GBV_ILN_24 | ||
912 | |a GBV_ILN_31 | ||
912 | |a GBV_ILN_32 | ||
912 | |a GBV_ILN_40 | ||
912 | |a GBV_ILN_60 | ||
912 | |a GBV_ILN_62 | ||
912 | |a GBV_ILN_63 | ||
912 | |a GBV_ILN_65 | ||
912 | |a GBV_ILN_69 | ||
912 | |a GBV_ILN_70 | ||
912 | |a GBV_ILN_73 | ||
912 | |a GBV_ILN_74 | ||
912 | |a GBV_ILN_90 | ||
912 | |a GBV_ILN_95 | ||
912 | |a GBV_ILN_100 | ||
912 | |a GBV_ILN_101 | ||
912 | |a GBV_ILN_105 | ||
912 | |a GBV_ILN_110 | ||
912 | |a GBV_ILN_151 | ||
912 | |a GBV_ILN_224 | ||
912 | |a GBV_ILN_370 | ||
912 | |a GBV_ILN_602 | ||
912 | |a GBV_ILN_702 | ||
912 | |a GBV_ILN_2003 | ||
912 | |a GBV_ILN_2004 | ||
912 | |a GBV_ILN_2005 | ||
912 | |a GBV_ILN_2011 | ||
912 | |a GBV_ILN_2014 | ||
912 | |a GBV_ILN_2015 | ||
912 | |a GBV_ILN_2020 | ||
912 | |a GBV_ILN_2021 | ||
912 | |a GBV_ILN_2025 | ||
912 | |a GBV_ILN_2027 | ||
912 | |a GBV_ILN_2034 | ||
912 | |a GBV_ILN_2038 | ||
912 | |a GBV_ILN_2044 | ||
912 | |a GBV_ILN_2048 | ||
912 | |a GBV_ILN_2049 | ||
912 | |a GBV_ILN_2050 | ||
912 | |a GBV_ILN_2056 | ||
912 | |a GBV_ILN_2059 | ||
912 | |a GBV_ILN_2061 | ||
912 | |a GBV_ILN_2064 | ||
912 | |a GBV_ILN_2065 | ||
912 | |a GBV_ILN_2068 | ||
912 | |a GBV_ILN_2111 | ||
912 | |a GBV_ILN_2112 | ||
912 | |a GBV_ILN_2113 | ||
912 | |a GBV_ILN_2118 | ||
912 | |a GBV_ILN_2122 | ||
912 | |a GBV_ILN_2129 | ||
912 | |a GBV_ILN_2143 | ||
912 | |a GBV_ILN_2147 | ||
912 | |a GBV_ILN_2148 | ||
912 | |a GBV_ILN_2152 | ||
912 | |a GBV_ILN_2153 | ||
912 | |a GBV_ILN_2190 | ||
912 | |a GBV_ILN_2336 | ||
912 | |a GBV_ILN_2507 | ||
912 | |a GBV_ILN_2522 | ||
912 | |a GBV_ILN_4035 | ||
912 | |a GBV_ILN_4037 | ||
912 | |a GBV_ILN_4112 | ||
912 | |a GBV_ILN_4125 | ||
912 | |a GBV_ILN_4126 | ||
912 | |a GBV_ILN_4242 | ||
912 | |a GBV_ILN_4251 | ||
912 | |a GBV_ILN_4305 | ||
912 | |a GBV_ILN_4313 | ||
912 | |a GBV_ILN_4323 | ||
912 | |a GBV_ILN_4324 | ||
912 | |a GBV_ILN_4325 | ||
912 | |a GBV_ILN_4326 | ||
912 | |a GBV_ILN_4333 | ||
912 | |a GBV_ILN_4334 | ||
912 | |a GBV_ILN_4335 | ||
912 | |a GBV_ILN_4338 | ||
912 | |a GBV_ILN_4393 | ||
936 | b | k | |a 48.68 |j Aquakultur |
951 | |a AR | ||
952 | |d 530 |
author_variant |
m a d ma mad i i ii m i mi m s ms s j sj a c ac |
---|---|
matchkey_str |
dietrichmariolaairnazarowilgizinglotmich:2020----:omnltmlto |
hierarchy_sort_str |
2020 |
bklnumber |
48.68 |
publishDate |
2020 |
allfields |
10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530 |
spelling |
10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530 |
allfields_unstemmed |
10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530 |
allfieldsGer |
10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530 |
allfieldsSound |
10.1016/j.aquaculture.2020.735791 doi (DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 DE-627 ger DE-627 rda eng 570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Dietrich, Mariola A. verfasserin aut Hormonal stimulation of carp ( 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics Irnazarow, Ilgiz verfasserin aut Inglot, Michał verfasserin aut Słowińska, Mariola verfasserin aut Judycka, Sylwia verfasserin aut Ciereszko, Andrzej verfasserin aut Enthalten in Aquaculture Amsterdam [u.a.] : Elsevier Science, 1972 530 Online-Ressource (DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 nnns volume:530 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 48.68 Aquakultur AR 530 |
language |
English |
source |
Enthalten in Aquaculture 530 volume:530 |
sourceStr |
Enthalten in Aquaculture 530 volume:530 |
format_phy_str_mv |
Article |
bklname |
Aquakultur |
institution |
findex.gbv.de |
topic_facet |
Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics |
dewey-raw |
570 |
isfreeaccess_bool |
false |
container_title |
Aquaculture |
authorswithroles_txt_mv |
Dietrich, Mariola A. @@aut@@ Irnazarow, Ilgiz @@aut@@ Inglot, Michał @@aut@@ Słowińska, Mariola @@aut@@ Judycka, Sylwia @@aut@@ Ciereszko, Andrzej @@aut@@ |
publishDateDaySort_date |
2020-01-01T00:00:00Z |
hierarchy_top_id |
306314002 |
dewey-sort |
3570 |
id |
ELV004843983 |
language_de |
englisch |
fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV004843983</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230524125554.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230503s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.aquaculture.2020.735791</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV004843983</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0044-8486(20)31153-4</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">550</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIODIV</subfield><subfield code="q">DE-30</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">48.68</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Dietrich, Mariola A.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Hormonal stimulation of carp (</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fish reproduction</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hormone</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Maturation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Spermatozoa</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Spermiation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proteomics</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Irnazarow, Ilgiz</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Inglot, Michał</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Słowińska, Mariola</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Judycka, Sylwia</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ciereszko, Andrzej</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Aquaculture</subfield><subfield code="d">Amsterdam [u.a.] : Elsevier Science, 1972</subfield><subfield code="g">530</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)306314002</subfield><subfield code="w">(DE-600)1495998-7</subfield><subfield code="w">(DE-576)094503966</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:530</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_101</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">48.68</subfield><subfield code="j">Aquakultur</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">530</subfield></datafield></record></collection>
|
author |
Dietrich, Mariola A. |
spellingShingle |
Dietrich, Mariola A. ddc 570 fid BIODIV bkl 48.68 misc Fish reproduction misc Hormone misc Maturation misc Spermatozoa misc Spermiation misc Proteomics Hormonal stimulation of carp ( |
authorStr |
Dietrich, Mariola A. |
ppnlink_with_tag_str_mv |
@@773@@(DE-627)306314002 |
format |
electronic Article |
dewey-ones |
570 - Life sciences; biology 550 - Earth sciences |
delete_txt_mv |
keep |
author_role |
aut aut aut aut aut aut |
collection |
elsevier |
remote_str |
true |
illustrated |
Not Illustrated |
topic_title |
570 550 DE-600 BIODIV DE-30 fid 48.68 bkl Hormonal stimulation of carp ( Fish reproduction Hormone Maturation Spermatozoa Spermiation Proteomics |
topic |
ddc 570 fid BIODIV bkl 48.68 misc Fish reproduction misc Hormone misc Maturation misc Spermatozoa misc Spermiation misc Proteomics |
topic_unstemmed |
ddc 570 fid BIODIV bkl 48.68 misc Fish reproduction misc Hormone misc Maturation misc Spermatozoa misc Spermiation misc Proteomics |
topic_browse |
ddc 570 fid BIODIV bkl 48.68 misc Fish reproduction misc Hormone misc Maturation misc Spermatozoa misc Spermiation misc Proteomics |
format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
format_main_str_mv |
Text Zeitschrift/Artikel |
carriertype_str_mv |
cr |
hierarchy_parent_title |
Aquaculture |
hierarchy_parent_id |
306314002 |
dewey-tens |
570 - Life sciences; biology 550 - Earth sciences & geology |
hierarchy_top_title |
Aquaculture |
isfreeaccess_txt |
false |
familylinks_str_mv |
(DE-627)306314002 (DE-600)1495998-7 (DE-576)094503966 |
title |
Hormonal stimulation of carp ( |
ctrlnum |
(DE-627)ELV004843983 (ELSEVIER)S0044-8486(20)31153-4 |
title_full |
Hormonal stimulation of carp ( |
author_sort |
Dietrich, Mariola A. |
journal |
Aquaculture |
journalStr |
Aquaculture |
lang_code |
eng |
isOA_bool |
false |
dewey-hundreds |
500 - Science |
recordtype |
marc |
publishDateSort |
2020 |
contenttype_str_mv |
zzz |
author_browse |
Dietrich, Mariola A. Irnazarow, Ilgiz Inglot, Michał Słowińska, Mariola Judycka, Sylwia Ciereszko, Andrzej |
container_volume |
530 |
class |
570 550 DE-600 BIODIV DE-30 fid 48.68 bkl |
format_se |
Elektronische Aufsätze |
author-letter |
Dietrich, Mariola A. |
doi_str_mv |
10.1016/j.aquaculture.2020.735791 |
dewey-full |
570 550 |
author2-role |
verfasserin |
title_sort |
hormonal stimulation of carp ( |
title_auth |
Hormonal stimulation of carp ( |
abstract |
Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. |
abstractGer |
Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. |
abstract_unstemmed |
Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049. |
collection_details |
GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 |
title_short |
Hormonal stimulation of carp ( |
remote_bool |
true |
author2 |
Irnazarow, Ilgiz Inglot, Michał Słowińska, Mariola Judycka, Sylwia Ciereszko, Andrzej |
author2Str |
Irnazarow, Ilgiz Inglot, Michał Słowińska, Mariola Judycka, Sylwia Ciereszko, Andrzej |
ppnlink |
306314002 |
mediatype_str_mv |
c |
isOA_txt |
false |
hochschulschrift_bool |
false |
doi_str |
10.1016/j.aquaculture.2020.735791 |
up_date |
2024-07-07T00:20:09.523Z |
_version_ |
1803877466269286400 |
fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV004843983</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230524125554.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">230503s2020 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.aquaculture.2020.735791</subfield><subfield code="2">doi</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV004843983</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0044-8486(20)31153-4</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rda</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="a">550</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">BIODIV</subfield><subfield code="q">DE-30</subfield><subfield code="2">fid</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">48.68</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Dietrich, Mariola A.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Hormonal stimulation of carp (</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2020</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">Computermedien</subfield><subfield code="b">c</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">Online-Ressource</subfield><subfield code="b">cr</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Hormonal stimulation of spermiation is the most commonly used method for carp kept in captivity. Recent studies have suggested that hormonal stimulation of carp resulted in changes of the seminal plasma proteome. However information regarding proteome changes in spermatozoa upon hormonal stimulation is lacking. Here, we aimed to compare the sperm quality parameters and spermatozoa proteomes of hormonally stimulated (injected with Ovopel) and nonstimulated (injected with PBS) males using a 2D-DIGE approach. Hormonal stimulation was accompanied by increases in sperm viability, sperm movement trajectory parameters and ATP level. No differences were observed in the percentage of sperm motility, velocity or the number of reactive oxygen species (ROS)-positive cells. Hormonal stimulation caused changes in abundance of 48 proteins and 12 phosphoproteins. The majority of these proteins are involved in cilium assembly and organization, energy metabolism, protein folding and signal transduction. The changes in phosphorylation status strongly suggest that sperm maturation is controlled through signal transduction via phosphorylation of the flagellar motile apparatus. For the first time, our results allowed a comprehensive characterization of carp spermatozoa proteins associated with final maturation after hormonal stimulation and allowed a better understanding of the complex mechanisms underlying hormone action in the male reproductive tract. The data have been deposited to the ProteomeXchange with identifier PXD011049.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Fish reproduction</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Hormone</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Maturation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Spermatozoa</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Spermiation</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Proteomics</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Irnazarow, Ilgiz</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Inglot, Michał</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Słowińska, Mariola</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Judycka, Sylwia</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Ciereszko, Andrzej</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="t">Aquaculture</subfield><subfield code="d">Amsterdam [u.a.] : Elsevier Science, 1972</subfield><subfield code="g">530</subfield><subfield code="h">Online-Ressource</subfield><subfield code="w">(DE-627)306314002</subfield><subfield code="w">(DE-600)1495998-7</subfield><subfield code="w">(DE-576)094503966</subfield><subfield code="7">nnns</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:530</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">FID-BIODIV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_20</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_23</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_24</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_31</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_32</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_60</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_62</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_63</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_65</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_69</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_70</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_73</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_74</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_90</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_95</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_100</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_101</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_110</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_151</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_224</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_370</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_602</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_702</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2003</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2004</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2005</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2011</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2014</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2015</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2020</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2021</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2025</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2027</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2034</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2038</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2044</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2048</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2049</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2050</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2056</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2059</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2061</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2064</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2065</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2068</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2111</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2113</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2118</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2122</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2129</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2143</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2147</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2148</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2152</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2153</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2190</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2336</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2507</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_2522</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4035</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4037</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4112</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4125</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4126</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4242</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4251</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4305</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4313</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4323</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4324</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4325</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4326</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4333</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4334</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4335</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4338</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_4393</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">48.68</subfield><subfield code="j">Aquakultur</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">530</subfield></datafield></record></collection>
|
score |
7.397958 |