A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation
Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiomet...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Zhu, Liping [verfasserIn] Yu, Linying [verfasserIn] Ye, Jing [verfasserIn] Yan, Mengxia [verfasserIn] Peng, Yao [verfasserIn] Huang, Jianshe [verfasserIn] Yang, Xiurong [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Biosensors and bioelectronics - Amsterdam [u.a.] : Elsevier Science, 1989, 178 |
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| Übergeordnetes Werk: |
volume:178 |
| DOI / URN: |
10.1016/j.bios.2021.113022 |
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| Katalog-ID: |
ELV005577454 |
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| 245 | 1 | 0 | |a A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation |
| 264 | 1 | |c 2021 | |
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| 337 | |a Computermedien |b c |2 rdamedia | ||
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| 520 | |a Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)3 2+ donor/acceptor pair. The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)3 2+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)3 2+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates (“OR”, “INHIBIT”, “AND”, “NAND” and “INHIBIT-OR”) based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+. | ||
| 650 | 4 | |a Electrochemiluminescence | |
| 650 | 4 | |a Hybridization chain reaction | |
| 650 | 4 | |a Resonance energy transfer | |
| 650 | 4 | |a Ratiometric biosensor | |
| 650 | 4 | |a Logic gates | |
| 700 | 1 | |a Yu, Linying |e verfasserin |4 aut | |
| 700 | 1 | |a Ye, Jing |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Mengxia |e verfasserin |4 aut | |
| 700 | 1 | |a Peng, Yao |e verfasserin |4 aut | |
| 700 | 1 | |a Huang, Jianshe |e verfasserin |4 aut | |
| 700 | 1 | |a Yang, Xiurong |e verfasserin |0 (orcid)0000-0003-0021-5135 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Biosensors and bioelectronics |d Amsterdam [u.a.] : Elsevier Science, 1989 |g 178 |h Online-Ressource |w (DE-627)30632122X |w (DE-600)1496379-6 |w (DE-576)094082596 |x 1873-4235 |7 nnns |
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| 912 | |a GBV_ILN_2003 | ||
| 912 | |a GBV_ILN_2004 | ||
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| 912 | |a GBV_ILN_2152 | ||
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| 912 | |a GBV_ILN_2190 | ||
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| 912 | |a GBV_ILN_4125 | ||
| 912 | |a GBV_ILN_4126 | ||
| 912 | |a GBV_ILN_4242 | ||
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| 912 | |a GBV_ILN_4305 | ||
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| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
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10.1016/j.bios.2021.113022 doi (DE-627)ELV005577454 (ELSEVIER)S0956-5663(21)00058-0 DE-627 ger DE-627 rda eng 570 610 DE-600 58.30 bkl 50.22 bkl 44.09 bkl Zhu, Liping verfasserin (orcid)0000-0002-6482-9286 aut A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)3 2+ donor/acceptor pair. The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)3 2+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)3 2+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates (“OR”, “INHIBIT”, “AND”, “NAND” and “INHIBIT-OR”) based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+. Electrochemiluminescence Hybridization chain reaction Resonance energy transfer Ratiometric biosensor Logic gates Yu, Linying verfasserin aut Ye, Jing verfasserin aut Yan, Mengxia verfasserin aut Peng, Yao verfasserin aut Huang, Jianshe verfasserin aut Yang, Xiurong verfasserin (orcid)0000-0003-0021-5135 aut Enthalten in Biosensors and bioelectronics Amsterdam [u.a.] : Elsevier Science, 1989 178 Online-Ressource (DE-627)30632122X (DE-600)1496379-6 (DE-576)094082596 1873-4235 nnns volume:178 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 50.22 Sensorik 44.09 Medizintechnik AR 178 |
| spelling |
10.1016/j.bios.2021.113022 doi (DE-627)ELV005577454 (ELSEVIER)S0956-5663(21)00058-0 DE-627 ger DE-627 rda eng 570 610 DE-600 58.30 bkl 50.22 bkl 44.09 bkl Zhu, Liping verfasserin (orcid)0000-0002-6482-9286 aut A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)3 2+ donor/acceptor pair. The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)3 2+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)3 2+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates (“OR”, “INHIBIT”, “AND”, “NAND” and “INHIBIT-OR”) based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+. Electrochemiluminescence Hybridization chain reaction Resonance energy transfer Ratiometric biosensor Logic gates Yu, Linying verfasserin aut Ye, Jing verfasserin aut Yan, Mengxia verfasserin aut Peng, Yao verfasserin aut Huang, Jianshe verfasserin aut Yang, Xiurong verfasserin (orcid)0000-0003-0021-5135 aut Enthalten in Biosensors and bioelectronics Amsterdam [u.a.] : Elsevier Science, 1989 178 Online-Ressource (DE-627)30632122X (DE-600)1496379-6 (DE-576)094082596 1873-4235 nnns volume:178 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 50.22 Sensorik 44.09 Medizintechnik AR 178 |
| allfields_unstemmed |
10.1016/j.bios.2021.113022 doi (DE-627)ELV005577454 (ELSEVIER)S0956-5663(21)00058-0 DE-627 ger DE-627 rda eng 570 610 DE-600 58.30 bkl 50.22 bkl 44.09 bkl Zhu, Liping verfasserin (orcid)0000-0002-6482-9286 aut A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)3 2+ donor/acceptor pair. The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)3 2+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)3 2+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates (“OR”, “INHIBIT”, “AND”, “NAND” and “INHIBIT-OR”) based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+. Electrochemiluminescence Hybridization chain reaction Resonance energy transfer Ratiometric biosensor Logic gates Yu, Linying verfasserin aut Ye, Jing verfasserin aut Yan, Mengxia verfasserin aut Peng, Yao verfasserin aut Huang, Jianshe verfasserin aut Yang, Xiurong verfasserin (orcid)0000-0003-0021-5135 aut Enthalten in Biosensors and bioelectronics Amsterdam [u.a.] : Elsevier Science, 1989 178 Online-Ressource (DE-627)30632122X (DE-600)1496379-6 (DE-576)094082596 1873-4235 nnns volume:178 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 50.22 Sensorik 44.09 Medizintechnik AR 178 |
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10.1016/j.bios.2021.113022 doi (DE-627)ELV005577454 (ELSEVIER)S0956-5663(21)00058-0 DE-627 ger DE-627 rda eng 570 610 DE-600 58.30 bkl 50.22 bkl 44.09 bkl Zhu, Liping verfasserin (orcid)0000-0002-6482-9286 aut A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)3 2+ donor/acceptor pair. The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)3 2+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)3 2+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates (“OR”, “INHIBIT”, “AND”, “NAND” and “INHIBIT-OR”) based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+. Electrochemiluminescence Hybridization chain reaction Resonance energy transfer Ratiometric biosensor Logic gates Yu, Linying verfasserin aut Ye, Jing verfasserin aut Yan, Mengxia verfasserin aut Peng, Yao verfasserin aut Huang, Jianshe verfasserin aut Yang, Xiurong verfasserin (orcid)0000-0003-0021-5135 aut Enthalten in Biosensors and bioelectronics Amsterdam [u.a.] : Elsevier Science, 1989 178 Online-Ressource (DE-627)30632122X (DE-600)1496379-6 (DE-576)094082596 1873-4235 nnns volume:178 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 50.22 Sensorik 44.09 Medizintechnik AR 178 |
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10.1016/j.bios.2021.113022 doi (DE-627)ELV005577454 (ELSEVIER)S0956-5663(21)00058-0 DE-627 ger DE-627 rda eng 570 610 DE-600 58.30 bkl 50.22 bkl 44.09 bkl Zhu, Liping verfasserin (orcid)0000-0002-6482-9286 aut A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)3 2+ donor/acceptor pair. The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)3 2+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)3 2+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates (“OR”, “INHIBIT”, “AND”, “NAND” and “INHIBIT-OR”) based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+. Electrochemiluminescence Hybridization chain reaction Resonance energy transfer Ratiometric biosensor Logic gates Yu, Linying verfasserin aut Ye, Jing verfasserin aut Yan, Mengxia verfasserin aut Peng, Yao verfasserin aut Huang, Jianshe verfasserin aut Yang, Xiurong verfasserin (orcid)0000-0003-0021-5135 aut Enthalten in Biosensors and bioelectronics Amsterdam [u.a.] : Elsevier Science, 1989 178 Online-Ressource (DE-627)30632122X (DE-600)1496379-6 (DE-576)094082596 1873-4235 nnns volume:178 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie 50.22 Sensorik 44.09 Medizintechnik AR 178 |
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Enthalten in Biosensors and bioelectronics 178 volume:178 |
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Electrochemiluminescence Hybridization chain reaction Resonance energy transfer Ratiometric biosensor Logic gates |
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Zhu, Liping @@aut@@ Yu, Linying @@aut@@ Ye, Jing @@aut@@ Yan, Mengxia @@aut@@ Peng, Yao @@aut@@ Huang, Jianshe @@aut@@ Yang, Xiurong @@aut@@ |
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Zhu, Liping ddc 570 bkl 58.30 bkl 50.22 bkl 44.09 misc Electrochemiluminescence misc Hybridization chain reaction misc Resonance energy transfer misc Ratiometric biosensor misc Logic gates A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation |
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570 610 DE-600 58.30 bkl 50.22 bkl 44.09 bkl A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation Electrochemiluminescence Hybridization chain reaction Resonance energy transfer Ratiometric biosensor Logic gates |
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a ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation |
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A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation |
| abstract |
Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)3 2+ donor/acceptor pair. The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)3 2+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)3 2+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates (“OR”, “INHIBIT”, “AND”, “NAND” and “INHIBIT-OR”) based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+. |
| abstractGer |
Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)3 2+ donor/acceptor pair. The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)3 2+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)3 2+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates (“OR”, “INHIBIT”, “AND”, “NAND” and “INHIBIT-OR”) based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+. |
| abstract_unstemmed |
Two-dimensional (2D) nanomaterial-nucleic acid interactions have been widely used in the construction of fluorescent sensors, but they are rarely used in the construction of electrochemiluminescent (ECL) sensors and have never been used in the design of ratiometric ECL sensors. Therefore, a ratiometric ECL sensing platform was developed in this study based on the ECL resonance energy transfer (ECL-RET) of graphitic carbon nitride nanosheets (GCNNs)/Ru(bpy)3 2+ donor/acceptor pair. The 2D GCNNs showed much weaker affinity to the long dsDNA duplexes formed by hybridization chain reaction (HCR) than Ru(bpy)3 2+-lableled fuel hairpin DNAs (H1 and H2) for HCR. Therefore, the target-initiated HCR resulted in the luminescence enhancement of the GCNNs at 460 nm and the luminescence attenuation of the Ru(bpy)3 2+ at 610 nm. By measuring the I460 nm/I610 nm ratios, quantitative analysis of microRNA-133a was realized with a limit of detection of 0.41 pM. In addition, this ECL-RET sensing platform can be easily extended to detect metal ions or aptamer substrates by simply redesigning helper DNAs without changing the sequences of fuel hairpin DNAs. Moreover, due to the programmability of HCR, a series of sensitive logic gates (“OR”, “INHIBIT”, “AND”, “NAND” and “INHIBIT-OR”) based on the ECL-RET ratiometry can be constructed and responded to as low as 100 pM of Hg2+ or Ag+. |
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A ratiometric electrochemiluminescence strategy based on two-dimensional nanomaterial-nucleic acid interactions for biosensing and logic gates operation |
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| score |
7.4002256 |