Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor

It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great sig...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Wang, Ge [verfasserIn] Yin, Pengxue [verfasserIn] Wang, Jin [verfasserIn] Ma, Pengxin [verfasserIn] Wang, Yanbo [verfasserIn] Cai, Yuanyuan [verfasserIn] Qi, Huan [verfasserIn] Liu, Aihua [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Specific heptapeptide screening pIII Phage display library Enzyme-linked chemiluminescence immunoassay Vascular endothelial growth factor Angiogenic factor

Übergeordnetes Werk:	Enthalten in: Sensors and actuators / B - Amsterdam [u.a.] : Elsevier Science, 1990, 333
Übergeordnetes Werk:	volume:333

DOI / URN:	10.1016/j.snb.2021.129555

Katalog-ID:	ELV00561693X

Internformat


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245	1	0	\|a Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor
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520			\|a It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great significance to detect VEGF in clinical diagnostics. Especially, the level of the isoform VEGF165 is usually called as the key index of many malignant tumors. Herein, phage monoclones bound to VEGF165 were selected from the pIII phage display library. Based on the phage capture assay, the phage monoclone expressing peptide LSTSPHT exhibited the best affinity and specificity against VEGF165. Then, peptide V (with the peptide sequence of LSTSPHTGGGSC) was synthesized as VEGF165 capturer to develop an enzyme-linked chemiluminescence immunoassay (ELCLIA). Under the optimal conditions, the proposed ELCLIA method showed wide linear range (10–1000 pg mL−1) and lower limit of detection (5.7 pg mL−1). Moreover, the as-developed ELCLIA was capable of detecting VEGF165 in human serum samples accurately and reproducibly. Due to its good selectivity, stability and cost-effectiveness, the VEGF165-specific peptide is prospected as a novel biointerface in sensing and tumor metastasis related diagnosis and therapy.
650		4	\|a Specific heptapeptide screening
650		4	\|a pIII Phage display library
650		4	\|a Enzyme-linked chemiluminescence immunoassay
650		4	\|a Vascular endothelial growth factor
650		4	\|a Angiogenic factor
700	1		\|a Yin, Pengxue \|e verfasserin \|4 aut
700	1		\|a Wang, Jin \|e verfasserin \|4 aut
700	1		\|a Ma, Pengxin \|e verfasserin \|4 aut
700	1		\|a Wang, Yanbo \|e verfasserin \|4 aut
700	1		\|a Cai, Yuanyuan \|e verfasserin \|4 aut
700	1		\|a Qi, Huan \|e verfasserin \|4 aut
700	1		\|a Liu, Aihua \|e verfasserin \|0 (orcid)0000-0003-4260-1982 \|4 aut
773	0	8	\|i Enthalten in \|t Sensors and actuators <Lausanne> / B \|d Amsterdam [u.a.] : Elsevier Science, 1990 \|g 333 \|h Online-Ressource \|w (DE-627)306710358 \|w (DE-600)1500731-5 \|w (DE-576)082435855 \|x 0925-4005 \|7 nnns
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936	b	k	\|a 50.22 \|j Sensorik
936	b	k	\|a 35.07 \|j Chemisches Labor \|j chemische Methoden
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publishDate	2021
allfields	10.1016/j.snb.2021.129555 doi (DE-627)ELV00561693X (ELSEVIER)S0925-4005(21)00123-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Wang, Ge verfasserin aut Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great significance to detect VEGF in clinical diagnostics. Especially, the level of the isoform VEGF165 is usually called as the key index of many malignant tumors. Herein, phage monoclones bound to VEGF165 were selected from the pIII phage display library. Based on the phage capture assay, the phage monoclone expressing peptide LSTSPHT exhibited the best affinity and specificity against VEGF165. Then, peptide V (with the peptide sequence of LSTSPHTGGGSC) was synthesized as VEGF165 capturer to develop an enzyme-linked chemiluminescence immunoassay (ELCLIA). Under the optimal conditions, the proposed ELCLIA method showed wide linear range (10–1000 pg mL−1) and lower limit of detection (5.7 pg mL−1). Moreover, the as-developed ELCLIA was capable of detecting VEGF165 in human serum samples accurately and reproducibly. Due to its good selectivity, stability and cost-effectiveness, the VEGF165-specific peptide is prospected as a novel biointerface in sensing and tumor metastasis related diagnosis and therapy. Specific heptapeptide screening pIII Phage display library Enzyme-linked chemiluminescence immunoassay Vascular endothelial growth factor Angiogenic factor Yin, Pengxue verfasserin aut Wang, Jin verfasserin aut Ma, Pengxin verfasserin aut Wang, Yanbo verfasserin aut Cai, Yuanyuan verfasserin aut Qi, Huan verfasserin aut Liu, Aihua verfasserin (orcid)0000-0003-4260-1982 aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 333 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:333 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 333
spelling	10.1016/j.snb.2021.129555 doi (DE-627)ELV00561693X (ELSEVIER)S0925-4005(21)00123-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Wang, Ge verfasserin aut Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great significance to detect VEGF in clinical diagnostics. Especially, the level of the isoform VEGF165 is usually called as the key index of many malignant tumors. Herein, phage monoclones bound to VEGF165 were selected from the pIII phage display library. Based on the phage capture assay, the phage monoclone expressing peptide LSTSPHT exhibited the best affinity and specificity against VEGF165. Then, peptide V (with the peptide sequence of LSTSPHTGGGSC) was synthesized as VEGF165 capturer to develop an enzyme-linked chemiluminescence immunoassay (ELCLIA). Under the optimal conditions, the proposed ELCLIA method showed wide linear range (10–1000 pg mL−1) and lower limit of detection (5.7 pg mL−1). Moreover, the as-developed ELCLIA was capable of detecting VEGF165 in human serum samples accurately and reproducibly. Due to its good selectivity, stability and cost-effectiveness, the VEGF165-specific peptide is prospected as a novel biointerface in sensing and tumor metastasis related diagnosis and therapy. Specific heptapeptide screening pIII Phage display library Enzyme-linked chemiluminescence immunoassay Vascular endothelial growth factor Angiogenic factor Yin, Pengxue verfasserin aut Wang, Jin verfasserin aut Ma, Pengxin verfasserin aut Wang, Yanbo verfasserin aut Cai, Yuanyuan verfasserin aut Qi, Huan verfasserin aut Liu, Aihua verfasserin (orcid)0000-0003-4260-1982 aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 333 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:333 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 333
allfields_unstemmed	10.1016/j.snb.2021.129555 doi (DE-627)ELV00561693X (ELSEVIER)S0925-4005(21)00123-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Wang, Ge verfasserin aut Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great significance to detect VEGF in clinical diagnostics. Especially, the level of the isoform VEGF165 is usually called as the key index of many malignant tumors. Herein, phage monoclones bound to VEGF165 were selected from the pIII phage display library. Based on the phage capture assay, the phage monoclone expressing peptide LSTSPHT exhibited the best affinity and specificity against VEGF165. Then, peptide V (with the peptide sequence of LSTSPHTGGGSC) was synthesized as VEGF165 capturer to develop an enzyme-linked chemiluminescence immunoassay (ELCLIA). Under the optimal conditions, the proposed ELCLIA method showed wide linear range (10–1000 pg mL−1) and lower limit of detection (5.7 pg mL−1). Moreover, the as-developed ELCLIA was capable of detecting VEGF165 in human serum samples accurately and reproducibly. Due to its good selectivity, stability and cost-effectiveness, the VEGF165-specific peptide is prospected as a novel biointerface in sensing and tumor metastasis related diagnosis and therapy. Specific heptapeptide screening pIII Phage display library Enzyme-linked chemiluminescence immunoassay Vascular endothelial growth factor Angiogenic factor Yin, Pengxue verfasserin aut Wang, Jin verfasserin aut Ma, Pengxin verfasserin aut Wang, Yanbo verfasserin aut Cai, Yuanyuan verfasserin aut Qi, Huan verfasserin aut Liu, Aihua verfasserin (orcid)0000-0003-4260-1982 aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 333 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:333 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 333
allfieldsGer	10.1016/j.snb.2021.129555 doi (DE-627)ELV00561693X (ELSEVIER)S0925-4005(21)00123-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Wang, Ge verfasserin aut Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great significance to detect VEGF in clinical diagnostics. Especially, the level of the isoform VEGF165 is usually called as the key index of many malignant tumors. Herein, phage monoclones bound to VEGF165 were selected from the pIII phage display library. Based on the phage capture assay, the phage monoclone expressing peptide LSTSPHT exhibited the best affinity and specificity against VEGF165. Then, peptide V (with the peptide sequence of LSTSPHTGGGSC) was synthesized as VEGF165 capturer to develop an enzyme-linked chemiluminescence immunoassay (ELCLIA). Under the optimal conditions, the proposed ELCLIA method showed wide linear range (10–1000 pg mL−1) and lower limit of detection (5.7 pg mL−1). Moreover, the as-developed ELCLIA was capable of detecting VEGF165 in human serum samples accurately and reproducibly. Due to its good selectivity, stability and cost-effectiveness, the VEGF165-specific peptide is prospected as a novel biointerface in sensing and tumor metastasis related diagnosis and therapy. Specific heptapeptide screening pIII Phage display library Enzyme-linked chemiluminescence immunoassay Vascular endothelial growth factor Angiogenic factor Yin, Pengxue verfasserin aut Wang, Jin verfasserin aut Ma, Pengxin verfasserin aut Wang, Yanbo verfasserin aut Cai, Yuanyuan verfasserin aut Qi, Huan verfasserin aut Liu, Aihua verfasserin (orcid)0000-0003-4260-1982 aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 333 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:333 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 333
allfieldsSound	10.1016/j.snb.2021.129555 doi (DE-627)ELV00561693X (ELSEVIER)S0925-4005(21)00123-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Wang, Ge verfasserin aut Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great significance to detect VEGF in clinical diagnostics. Especially, the level of the isoform VEGF165 is usually called as the key index of many malignant tumors. Herein, phage monoclones bound to VEGF165 were selected from the pIII phage display library. Based on the phage capture assay, the phage monoclone expressing peptide LSTSPHT exhibited the best affinity and specificity against VEGF165. Then, peptide V (with the peptide sequence of LSTSPHTGGGSC) was synthesized as VEGF165 capturer to develop an enzyme-linked chemiluminescence immunoassay (ELCLIA). Under the optimal conditions, the proposed ELCLIA method showed wide linear range (10–1000 pg mL−1) and lower limit of detection (5.7 pg mL−1). Moreover, the as-developed ELCLIA was capable of detecting VEGF165 in human serum samples accurately and reproducibly. Due to its good selectivity, stability and cost-effectiveness, the VEGF165-specific peptide is prospected as a novel biointerface in sensing and tumor metastasis related diagnosis and therapy. Specific heptapeptide screening pIII Phage display library Enzyme-linked chemiluminescence immunoassay Vascular endothelial growth factor Angiogenic factor Yin, Pengxue verfasserin aut Wang, Jin verfasserin aut Ma, Pengxin verfasserin aut Wang, Yanbo verfasserin aut Cai, Yuanyuan verfasserin aut Qi, Huan verfasserin aut Liu, Aihua verfasserin (orcid)0000-0003-4260-1982 aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 333 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:333 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 333
language	English
source	Enthalten in Sensors and actuators <Lausanne> / B 333 volume:333
sourceStr	Enthalten in Sensors and actuators <Lausanne> / B 333 volume:333
format_phy_str_mv	Article
bklname	Sensorik Chemisches Labor chemische Methoden
institution	findex.gbv.de
topic_facet	Specific heptapeptide screening pIII Phage display library Enzyme-linked chemiluminescence immunoassay Vascular endothelial growth factor Angiogenic factor
dewey-raw	530
isfreeaccess_bool	false
container_title	Sensors and actuators <Lausanne> / B
authorswithroles_txt_mv	Wang, Ge @@aut@@ Yin, Pengxue @@aut@@ Wang, Jin @@aut@@ Ma, Pengxin @@aut@@ Wang, Yanbo @@aut@@ Cai, Yuanyuan @@aut@@ Qi, Huan @@aut@@ Liu, Aihua @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	306710358
dewey-sort	3530
id	ELV00561693X
language_de	englisch
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author	Wang, Ge
spellingShingle	Wang, Ge ddc 530 bkl 50.22 bkl 35.07 misc Specific heptapeptide screening misc pIII Phage display library misc Enzyme-linked chemiluminescence immunoassay misc Vascular endothelial growth factor misc Angiogenic factor Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor
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topic_title	530 620 DE-600 50.22 bkl 35.07 bkl Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor Specific heptapeptide screening pIII Phage display library Enzyme-linked chemiluminescence immunoassay Vascular endothelial growth factor Angiogenic factor
topic	ddc 530 bkl 50.22 bkl 35.07 misc Specific heptapeptide screening misc pIII Phage display library misc Enzyme-linked chemiluminescence immunoassay misc Vascular endothelial growth factor misc Angiogenic factor
topic_unstemmed	ddc 530 bkl 50.22 bkl 35.07 misc Specific heptapeptide screening misc pIII Phage display library misc Enzyme-linked chemiluminescence immunoassay misc Vascular endothelial growth factor misc Angiogenic factor
topic_browse	ddc 530 bkl 50.22 bkl 35.07 misc Specific heptapeptide screening misc pIII Phage display library misc Enzyme-linked chemiluminescence immunoassay misc Vascular endothelial growth factor misc Angiogenic factor
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title	Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor
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title_full	Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor
author_sort	Wang, Ge
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author_browse	Wang, Ge Yin, Pengxue Wang, Jin Ma, Pengxin Wang, Yanbo Cai, Yuanyuan Qi, Huan Liu, Aihua
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title_sort	specific heptapeptide screened from piii phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor
title_auth	Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor
abstract	It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great significance to detect VEGF in clinical diagnostics. Especially, the level of the isoform VEGF165 is usually called as the key index of many malignant tumors. Herein, phage monoclones bound to VEGF165 were selected from the pIII phage display library. Based on the phage capture assay, the phage monoclone expressing peptide LSTSPHT exhibited the best affinity and specificity against VEGF165. Then, peptide V (with the peptide sequence of LSTSPHTGGGSC) was synthesized as VEGF165 capturer to develop an enzyme-linked chemiluminescence immunoassay (ELCLIA). Under the optimal conditions, the proposed ELCLIA method showed wide linear range (10–1000 pg mL−1) and lower limit of detection (5.7 pg mL−1). Moreover, the as-developed ELCLIA was capable of detecting VEGF165 in human serum samples accurately and reproducibly. Due to its good selectivity, stability and cost-effectiveness, the VEGF165-specific peptide is prospected as a novel biointerface in sensing and tumor metastasis related diagnosis and therapy.
abstractGer	It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great significance to detect VEGF in clinical diagnostics. Especially, the level of the isoform VEGF165 is usually called as the key index of many malignant tumors. Herein, phage monoclones bound to VEGF165 were selected from the pIII phage display library. Based on the phage capture assay, the phage monoclone expressing peptide LSTSPHT exhibited the best affinity and specificity against VEGF165. Then, peptide V (with the peptide sequence of LSTSPHTGGGSC) was synthesized as VEGF165 capturer to develop an enzyme-linked chemiluminescence immunoassay (ELCLIA). Under the optimal conditions, the proposed ELCLIA method showed wide linear range (10–1000 pg mL−1) and lower limit of detection (5.7 pg mL−1). Moreover, the as-developed ELCLIA was capable of detecting VEGF165 in human serum samples accurately and reproducibly. Due to its good selectivity, stability and cost-effectiveness, the VEGF165-specific peptide is prospected as a novel biointerface in sensing and tumor metastasis related diagnosis and therapy.
abstract_unstemmed	It is challenging to explore specific, stable and cost-effective biorecognizing elements for a wide range of applications including biosensing and nanomedicine. As the primary pro-angiogenic factor, vascular endothelial growth factor (VEGF) plays crucial roles in tumor metastasis. It holds great significance to detect VEGF in clinical diagnostics. Especially, the level of the isoform VEGF165 is usually called as the key index of many malignant tumors. Herein, phage monoclones bound to VEGF165 were selected from the pIII phage display library. Based on the phage capture assay, the phage monoclone expressing peptide LSTSPHT exhibited the best affinity and specificity against VEGF165. Then, peptide V (with the peptide sequence of LSTSPHTGGGSC) was synthesized as VEGF165 capturer to develop an enzyme-linked chemiluminescence immunoassay (ELCLIA). Under the optimal conditions, the proposed ELCLIA method showed wide linear range (10–1000 pg mL−1) and lower limit of detection (5.7 pg mL−1). Moreover, the as-developed ELCLIA was capable of detecting VEGF165 in human serum samples accurately and reproducibly. Due to its good selectivity, stability and cost-effectiveness, the VEGF165-specific peptide is prospected as a novel biointerface in sensing and tumor metastasis related diagnosis and therapy.
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title_short	Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor
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author2	Yin, Pengxue Wang, Jin Ma, Pengxin Wang, Yanbo Cai, Yuanyuan Qi, Huan Liu, Aihua
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up_date	2024-07-06T18:34:42.939Z
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Specific heptapeptide screened from pIII phage display library for sensitive enzyme-linked chemiluminescence immunoassay of vascular endothelial growth factor

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