Rapid Visualized Detection of

Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplificatio...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	ZHANG, Tong [verfasserIn] TAO, Qing [verfasserIn] BIAN, Xiao-Jun [verfasserIn] CHEN, Qian [verfasserIn] YAN, Juan [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Rolling circle amplification DNA hydrogel Aptamer Food-borne illness Food safety

Übergeordnetes Werk:	Enthalten in: Chinese journal of analytical chemistry - Changchun : Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 2006, 49, Seite 377-386
Übergeordnetes Werk:	volume:49 ; pages:377-386

DOI / URN:	10.1016/S1872-2040(21)60085-3

Katalog-ID:	ELV005657288

Internformat


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100	1		\|a ZHANG, Tong \|e verfasserin \|4 aut
245	1	0	\|a Rapid Visualized Detection of
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520			\|a Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Firstly, the Mbsdouble-stranded DNA (dsDNA) complexes were prepared. In the presence of target, the aptamer combined with E. coli O157:H7, releasing E. coli aptamer-initiator (EA-I). The supernatant collected by magnetic separation and those released primers were hybridized with circular sequence to form padlock probe. Then, RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase, and deoxynucleotides (dNTPs), while RCA cannot be triggered in the absence of target. Two circular sequences were designed to contain partially complementary bases. After RCA, two long single-stranded DNA (ssDNA) products were generated using those two circular sequences. They hybridized with each other and formed the naked-eye visible DNA hydrogel. The method showed high sensitivity and specificity with a detection limit of 4 × 103 CFU/mL for E. coli O157:H7 within 1 h, and the detection time could be finished within 30 min. The aptasensor offers many advantages such as high specificity, easy operation, efficient amplification, rapid and visualized readout, which has potential applications in food safety detection
650		4	\|a Rolling circle amplification
650		4	\|a DNA hydrogel
650		4	\|a Aptamer
650		4	\|a Food-borne illness
650		4	\|a Food safety
700	1		\|a TAO, Qing \|e verfasserin \|4 aut
700	1		\|a BIAN, Xiao-Jun \|e verfasserin \|4 aut
700	1		\|a CHEN, Qian \|e verfasserin \|4 aut
700	1		\|a YAN, Juan \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Chinese journal of analytical chemistry \|d Changchun : Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 2006 \|g 49, Seite 377-386 \|h Online-Ressource \|w (DE-627)552254266 \|w (DE-600)2399980-9 \|w (DE-576)284927163 \|x 1872-2040 \|7 nnns
773	1	8	\|g volume:49 \|g pages:377-386
912			\|a GBV_USEFLAG_U
912			\|a SYSFLAG_U
912			\|a GBV_ELV
912			\|a SSG-OLC-PHA
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2009
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2232
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
951			\|a AR
952			\|d 49 \|h 377-386

Indexfelder

author_variant	t z tz q t qt x j b xjb q c qc j y jy
matchkey_str	article:18722040:2021----::aivsaiedt
hierarchy_sort_str	2021
publishDate	2021
allfields	10.1016/S1872-2040(21)60085-3 doi (DE-627)ELV005657288 (ELSEVIER)S1872-2040(21)60085-3 DE-627 ger DE-627 rda eng 540 DE-600 ZHANG, Tong verfasserin aut Rapid Visualized Detection of 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Firstly, the Mbsdouble-stranded DNA (dsDNA) complexes were prepared. In the presence of target, the aptamer combined with E. coli O157:H7, releasing E. coli aptamer-initiator (EA-I). The supernatant collected by magnetic separation and those released primers were hybridized with circular sequence to form padlock probe. Then, RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase, and deoxynucleotides (dNTPs), while RCA cannot be triggered in the absence of target. Two circular sequences were designed to contain partially complementary bases. After RCA, two long single-stranded DNA (ssDNA) products were generated using those two circular sequences. They hybridized with each other and formed the naked-eye visible DNA hydrogel. The method showed high sensitivity and specificity with a detection limit of 4 × 103 CFU/mL for E. coli O157:H7 within 1 h, and the detection time could be finished within 30 min. The aptasensor offers many advantages such as high specificity, easy operation, efficient amplification, rapid and visualized readout, which has potential applications in food safety detection Rolling circle amplification DNA hydrogel Aptamer Food-borne illness Food safety TAO, Qing verfasserin aut BIAN, Xiao-Jun verfasserin aut CHEN, Qian verfasserin aut YAN, Juan verfasserin aut Enthalten in Chinese journal of analytical chemistry Changchun : Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 2006 49, Seite 377-386 Online-Ressource (DE-627)552254266 (DE-600)2399980-9 (DE-576)284927163 1872-2040 nnns volume:49 pages:377-386 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 49 377-386
spelling	10.1016/S1872-2040(21)60085-3 doi (DE-627)ELV005657288 (ELSEVIER)S1872-2040(21)60085-3 DE-627 ger DE-627 rda eng 540 DE-600 ZHANG, Tong verfasserin aut Rapid Visualized Detection of 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Firstly, the Mbsdouble-stranded DNA (dsDNA) complexes were prepared. In the presence of target, the aptamer combined with E. coli O157:H7, releasing E. coli aptamer-initiator (EA-I). The supernatant collected by magnetic separation and those released primers were hybridized with circular sequence to form padlock probe. Then, RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase, and deoxynucleotides (dNTPs), while RCA cannot be triggered in the absence of target. Two circular sequences were designed to contain partially complementary bases. After RCA, two long single-stranded DNA (ssDNA) products were generated using those two circular sequences. They hybridized with each other and formed the naked-eye visible DNA hydrogel. The method showed high sensitivity and specificity with a detection limit of 4 × 103 CFU/mL for E. coli O157:H7 within 1 h, and the detection time could be finished within 30 min. The aptasensor offers many advantages such as high specificity, easy operation, efficient amplification, rapid and visualized readout, which has potential applications in food safety detection Rolling circle amplification DNA hydrogel Aptamer Food-borne illness Food safety TAO, Qing verfasserin aut BIAN, Xiao-Jun verfasserin aut CHEN, Qian verfasserin aut YAN, Juan verfasserin aut Enthalten in Chinese journal of analytical chemistry Changchun : Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 2006 49, Seite 377-386 Online-Ressource (DE-627)552254266 (DE-600)2399980-9 (DE-576)284927163 1872-2040 nnns volume:49 pages:377-386 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 49 377-386
allfields_unstemmed	10.1016/S1872-2040(21)60085-3 doi (DE-627)ELV005657288 (ELSEVIER)S1872-2040(21)60085-3 DE-627 ger DE-627 rda eng 540 DE-600 ZHANG, Tong verfasserin aut Rapid Visualized Detection of 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Firstly, the Mbsdouble-stranded DNA (dsDNA) complexes were prepared. In the presence of target, the aptamer combined with E. coli O157:H7, releasing E. coli aptamer-initiator (EA-I). The supernatant collected by magnetic separation and those released primers were hybridized with circular sequence to form padlock probe. Then, RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase, and deoxynucleotides (dNTPs), while RCA cannot be triggered in the absence of target. Two circular sequences were designed to contain partially complementary bases. After RCA, two long single-stranded DNA (ssDNA) products were generated using those two circular sequences. They hybridized with each other and formed the naked-eye visible DNA hydrogel. The method showed high sensitivity and specificity with a detection limit of 4 × 103 CFU/mL for E. coli O157:H7 within 1 h, and the detection time could be finished within 30 min. The aptasensor offers many advantages such as high specificity, easy operation, efficient amplification, rapid and visualized readout, which has potential applications in food safety detection Rolling circle amplification DNA hydrogel Aptamer Food-borne illness Food safety TAO, Qing verfasserin aut BIAN, Xiao-Jun verfasserin aut CHEN, Qian verfasserin aut YAN, Juan verfasserin aut Enthalten in Chinese journal of analytical chemistry Changchun : Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 2006 49, Seite 377-386 Online-Ressource (DE-627)552254266 (DE-600)2399980-9 (DE-576)284927163 1872-2040 nnns volume:49 pages:377-386 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 49 377-386
allfieldsGer	10.1016/S1872-2040(21)60085-3 doi (DE-627)ELV005657288 (ELSEVIER)S1872-2040(21)60085-3 DE-627 ger DE-627 rda eng 540 DE-600 ZHANG, Tong verfasserin aut Rapid Visualized Detection of 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Firstly, the Mbsdouble-stranded DNA (dsDNA) complexes were prepared. In the presence of target, the aptamer combined with E. coli O157:H7, releasing E. coli aptamer-initiator (EA-I). The supernatant collected by magnetic separation and those released primers were hybridized with circular sequence to form padlock probe. Then, RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase, and deoxynucleotides (dNTPs), while RCA cannot be triggered in the absence of target. Two circular sequences were designed to contain partially complementary bases. After RCA, two long single-stranded DNA (ssDNA) products were generated using those two circular sequences. They hybridized with each other and formed the naked-eye visible DNA hydrogel. The method showed high sensitivity and specificity with a detection limit of 4 × 103 CFU/mL for E. coli O157:H7 within 1 h, and the detection time could be finished within 30 min. The aptasensor offers many advantages such as high specificity, easy operation, efficient amplification, rapid and visualized readout, which has potential applications in food safety detection Rolling circle amplification DNA hydrogel Aptamer Food-borne illness Food safety TAO, Qing verfasserin aut BIAN, Xiao-Jun verfasserin aut CHEN, Qian verfasserin aut YAN, Juan verfasserin aut Enthalten in Chinese journal of analytical chemistry Changchun : Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 2006 49, Seite 377-386 Online-Ressource (DE-627)552254266 (DE-600)2399980-9 (DE-576)284927163 1872-2040 nnns volume:49 pages:377-386 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 49 377-386
allfieldsSound	10.1016/S1872-2040(21)60085-3 doi (DE-627)ELV005657288 (ELSEVIER)S1872-2040(21)60085-3 DE-627 ger DE-627 rda eng 540 DE-600 ZHANG, Tong verfasserin aut Rapid Visualized Detection of 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Firstly, the Mbsdouble-stranded DNA (dsDNA) complexes were prepared. In the presence of target, the aptamer combined with E. coli O157:H7, releasing E. coli aptamer-initiator (EA-I). The supernatant collected by magnetic separation and those released primers were hybridized with circular sequence to form padlock probe. Then, RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase, and deoxynucleotides (dNTPs), while RCA cannot be triggered in the absence of target. Two circular sequences were designed to contain partially complementary bases. After RCA, two long single-stranded DNA (ssDNA) products were generated using those two circular sequences. They hybridized with each other and formed the naked-eye visible DNA hydrogel. The method showed high sensitivity and specificity with a detection limit of 4 × 103 CFU/mL for E. coli O157:H7 within 1 h, and the detection time could be finished within 30 min. The aptasensor offers many advantages such as high specificity, easy operation, efficient amplification, rapid and visualized readout, which has potential applications in food safety detection Rolling circle amplification DNA hydrogel Aptamer Food-borne illness Food safety TAO, Qing verfasserin aut BIAN, Xiao-Jun verfasserin aut CHEN, Qian verfasserin aut YAN, Juan verfasserin aut Enthalten in Chinese journal of analytical chemistry Changchun : Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, 2006 49, Seite 377-386 Online-Ressource (DE-627)552254266 (DE-600)2399980-9 (DE-576)284927163 1872-2040 nnns volume:49 pages:377-386 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 49 377-386
language	English
source	Enthalten in Chinese journal of analytical chemistry 49, Seite 377-386 volume:49 pages:377-386
sourceStr	Enthalten in Chinese journal of analytical chemistry 49, Seite 377-386 volume:49 pages:377-386
format_phy_str_mv	Article
institution	findex.gbv.de
topic_facet	Rolling circle amplification DNA hydrogel Aptamer Food-borne illness Food safety
dewey-raw	540
isfreeaccess_bool	false
container_title	Chinese journal of analytical chemistry
authorswithroles_txt_mv	ZHANG, Tong @@aut@@ TAO, Qing @@aut@@ BIAN, Xiao-Jun @@aut@@ CHEN, Qian @@aut@@ YAN, Juan @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	552254266
dewey-sort	3540
id	ELV005657288
language_de	englisch
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author	ZHANG, Tong
spellingShingle	ZHANG, Tong ddc 540 misc Rolling circle amplification misc DNA hydrogel misc Aptamer misc Food-borne illness misc Food safety Rapid Visualized Detection of
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topic_title	540 DE-600 Rapid Visualized Detection of Rolling circle amplification DNA hydrogel Aptamer Food-borne illness Food safety
topic	ddc 540 misc Rolling circle amplification misc DNA hydrogel misc Aptamer misc Food-borne illness misc Food safety
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title_sort	rapid visualized detection of
title_auth	Rapid Visualized Detection of
abstract	Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Firstly, the Mbsdouble-stranded DNA (dsDNA) complexes were prepared. In the presence of target, the aptamer combined with E. coli O157:H7, releasing E. coli aptamer-initiator (EA-I). The supernatant collected by magnetic separation and those released primers were hybridized with circular sequence to form padlock probe. Then, RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase, and deoxynucleotides (dNTPs), while RCA cannot be triggered in the absence of target. Two circular sequences were designed to contain partially complementary bases. After RCA, two long single-stranded DNA (ssDNA) products were generated using those two circular sequences. They hybridized with each other and formed the naked-eye visible DNA hydrogel. The method showed high sensitivity and specificity with a detection limit of 4 × 103 CFU/mL for E. coli O157:H7 within 1 h, and the detection time could be finished within 30 min. The aptasensor offers many advantages such as high specificity, easy operation, efficient amplification, rapid and visualized readout, which has potential applications in food safety detection
abstractGer	Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Firstly, the Mbsdouble-stranded DNA (dsDNA) complexes were prepared. In the presence of target, the aptamer combined with E. coli O157:H7, releasing E. coli aptamer-initiator (EA-I). The supernatant collected by magnetic separation and those released primers were hybridized with circular sequence to form padlock probe. Then, RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase, and deoxynucleotides (dNTPs), while RCA cannot be triggered in the absence of target. Two circular sequences were designed to contain partially complementary bases. After RCA, two long single-stranded DNA (ssDNA) products were generated using those two circular sequences. They hybridized with each other and formed the naked-eye visible DNA hydrogel. The method showed high sensitivity and specificity with a detection limit of 4 × 103 CFU/mL for E. coli O157:H7 within 1 h, and the detection time could be finished within 30 min. The aptasensor offers many advantages such as high specificity, easy operation, efficient amplification, rapid and visualized readout, which has potential applications in food safety detection
abstract_unstemmed	Food-borne illnesses caused by pathogenic bacteria are prominent issues in food safety. Rapid and sensitive detection of pathogenic bacteria is significantly essential for food safety. In this work, an aptamer biosensor (aptasensor) based on aldehyde magnetic beads (Mbs), rolling circle amplification (RCA) and DNA hydrogel was developed for visualized, simple and rapid detection of Escherichia coli O157:H7 (E. coli O157:H7). Firstly, the Mbsdouble-stranded DNA (dsDNA) complexes were prepared. In the presence of target, the aptamer combined with E. coli O157:H7, releasing E. coli aptamer-initiator (EA-I). The supernatant collected by magnetic separation and those released primers were hybridized with circular sequence to form padlock probe. Then, RCA reaction was initiated by adding T4 DNA ligase, phi29 DNA polymerase, and deoxynucleotides (dNTPs), while RCA cannot be triggered in the absence of target. Two circular sequences were designed to contain partially complementary bases. After RCA, two long single-stranded DNA (ssDNA) products were generated using those two circular sequences. They hybridized with each other and formed the naked-eye visible DNA hydrogel. The method showed high sensitivity and specificity with a detection limit of 4 × 103 CFU/mL for E. coli O157:H7 within 1 h, and the detection time could be finished within 30 min. The aptasensor offers many advantages such as high specificity, easy operation, efficient amplification, rapid and visualized readout, which has potential applications in food safety detection
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title_short	Rapid Visualized Detection of
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