Identification of the mutation signature of the cancer genome caused by irradiation
Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kageyama, Shun-Ichiro [verfasserIn] Du, Junyan [verfasserIn] Kaneko, Syuzo [verfasserIn] Hamamoto, Ryuji [verfasserIn] Yamaguchi, Shigeo [verfasserIn] Yamashita, Riu [verfasserIn] Okumura, Masayuki [verfasserIn] Motegi, Atsushi [verfasserIn] Hojo, Hidehiro [verfasserIn] Nakamura, Masaki [verfasserIn] Tsuchihara, Katsuya [verfasserIn] Akimoto, Tetsuo [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Radiotherapy and oncology - Amsterdam [u.a.] : Elsevier Science, 1983, 155, Seite 10-16 |
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| Übergeordnetes Werk: |
volume:155 ; pages:10-16 |
| DOI / URN: |
10.1016/j.radonc.2020.10.020 |
|---|
| Katalog-ID: |
ELV005692598 |
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| 024 | 7 | |a 10.1016/j.radonc.2020.10.020 |2 doi | |
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| 100 | 1 | |a Kageyama, Shun-Ichiro |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Identification of the mutation signature of the cancer genome caused by irradiation |
| 264 | 1 | |c 2020 | |
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| 337 | |a Computermedien |b c |2 rdamedia | ||
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| 520 | |a Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experimental system to analyse the presence of de novo mutations in the cancer genome of irradiated (60 Gy/20 fr/4 weeks) oesophageal cancer cell lines. Subsequently, we performed whole-genome, chromatin immunoprecipitation, and RNA sequencing using untreated and irradiated samples to assess the damage to the genome caused by radiation and understand the underlying mechanism.Results: The irradiated cancer cells exhibited hotspots for the de novo 8502–12966 single nucleotide variants and 954–1,331 indels on the chromosome. These single nucleotide variants primarily originated from double-stranded break repair errors, as determined using mutation signature analysis. The hotspots partially overlapped with the sites of H3K9 trimethylation, which are regions characterised by a weak capacity for double-stranded break repair.Conclusion: This study highlights the signature and underlying mechanism of radiation on the cancer genome. | ||
| 650 | 4 | |a Irradiation | |
| 650 | 4 | |a Radiotherapy | |
| 650 | 4 | |a Whole genome sequence | |
| 650 | 4 | |a Cancer genome | |
| 650 | 4 | |a Mutation signature | |
| 650 | 4 | |a Mutation hot spot | |
| 700 | 1 | |a Du, Junyan |e verfasserin |0 (orcid)0000-0003-1440-5538 |4 aut | |
| 700 | 1 | |a Kaneko, Syuzo |e verfasserin |4 aut | |
| 700 | 1 | |a Hamamoto, Ryuji |e verfasserin |0 (orcid)0000-0002-2632-1334 |4 aut | |
| 700 | 1 | |a Yamaguchi, Shigeo |e verfasserin |4 aut | |
| 700 | 1 | |a Yamashita, Riu |e verfasserin |4 aut | |
| 700 | 1 | |a Okumura, Masayuki |e verfasserin |0 (orcid)0000-0002-9842-165X |4 aut | |
| 700 | 1 | |a Motegi, Atsushi |e verfasserin |4 aut | |
| 700 | 1 | |a Hojo, Hidehiro |e verfasserin |4 aut | |
| 700 | 1 | |a Nakamura, Masaki |e verfasserin |0 (orcid)0000-0002-4431-8892 |4 aut | |
| 700 | 1 | |a Tsuchihara, Katsuya |e verfasserin |4 aut | |
| 700 | 1 | |a Akimoto, Tetsuo |e verfasserin |0 (orcid)0000-0003-0881-1345 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Radiotherapy and oncology |d Amsterdam [u.a.] : Elsevier Science, 1983 |g 155, Seite 10-16 |h Online-Ressource |w (DE-627)306710110 |w (DE-600)1500707-8 |w (DE-576)082435731 |x 1879-0887 |7 nnns |
| 773 | 1 | 8 | |g volume:155 |g pages:10-16 |
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| 912 | |a GBV_ILN_110 | ||
| 912 | |a GBV_ILN_151 | ||
| 912 | |a GBV_ILN_224 | ||
| 912 | |a GBV_ILN_370 | ||
| 912 | |a GBV_ILN_602 | ||
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| 912 | |a GBV_ILN_2003 | ||
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| 912 | |a GBV_ILN_2068 | ||
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| 912 | |a GBV_ILN_2522 | ||
| 912 | |a GBV_ILN_4035 | ||
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| 912 | |a GBV_ILN_4112 | ||
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| 912 | |a GBV_ILN_4242 | ||
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| 912 | |a GBV_ILN_4326 | ||
| 912 | |a GBV_ILN_4333 | ||
| 912 | |a GBV_ILN_4334 | ||
| 912 | |a GBV_ILN_4335 | ||
| 912 | |a GBV_ILN_4338 | ||
| 912 | |a GBV_ILN_4393 | ||
| 936 | b | k | |a 44.81 |j Onkologie |
| 936 | b | k | |a 44.64 |j Radiologie |
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2020 |
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44.81 44.64 |
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2020 |
| allfields |
10.1016/j.radonc.2020.10.020 doi (DE-627)ELV005692598 (ELSEVIER)S0167-8140(20)30856-2 DE-627 ger DE-627 rda eng 610 DE-600 44.81 bkl 44.64 bkl Kageyama, Shun-Ichiro verfasserin aut Identification of the mutation signature of the cancer genome caused by irradiation 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experimental system to analyse the presence of de novo mutations in the cancer genome of irradiated (60 Gy/20 fr/4 weeks) oesophageal cancer cell lines. Subsequently, we performed whole-genome, chromatin immunoprecipitation, and RNA sequencing using untreated and irradiated samples to assess the damage to the genome caused by radiation and understand the underlying mechanism.Results: The irradiated cancer cells exhibited hotspots for the de novo 8502–12966 single nucleotide variants and 954–1,331 indels on the chromosome. These single nucleotide variants primarily originated from double-stranded break repair errors, as determined using mutation signature analysis. The hotspots partially overlapped with the sites of H3K9 trimethylation, which are regions characterised by a weak capacity for double-stranded break repair.Conclusion: This study highlights the signature and underlying mechanism of radiation on the cancer genome. Irradiation Radiotherapy Whole genome sequence Cancer genome Mutation signature Mutation hot spot Du, Junyan verfasserin (orcid)0000-0003-1440-5538 aut Kaneko, Syuzo verfasserin aut Hamamoto, Ryuji verfasserin (orcid)0000-0002-2632-1334 aut Yamaguchi, Shigeo verfasserin aut Yamashita, Riu verfasserin aut Okumura, Masayuki verfasserin (orcid)0000-0002-9842-165X aut Motegi, Atsushi verfasserin aut Hojo, Hidehiro verfasserin aut Nakamura, Masaki verfasserin (orcid)0000-0002-4431-8892 aut Tsuchihara, Katsuya verfasserin aut Akimoto, Tetsuo verfasserin (orcid)0000-0003-0881-1345 aut Enthalten in Radiotherapy and oncology Amsterdam [u.a.] : Elsevier Science, 1983 155, Seite 10-16 Online-Ressource (DE-627)306710110 (DE-600)1500707-8 (DE-576)082435731 1879-0887 nnns volume:155 pages:10-16 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.81 Onkologie 44.64 Radiologie AR 155 10-16 |
| spelling |
10.1016/j.radonc.2020.10.020 doi (DE-627)ELV005692598 (ELSEVIER)S0167-8140(20)30856-2 DE-627 ger DE-627 rda eng 610 DE-600 44.81 bkl 44.64 bkl Kageyama, Shun-Ichiro verfasserin aut Identification of the mutation signature of the cancer genome caused by irradiation 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experimental system to analyse the presence of de novo mutations in the cancer genome of irradiated (60 Gy/20 fr/4 weeks) oesophageal cancer cell lines. Subsequently, we performed whole-genome, chromatin immunoprecipitation, and RNA sequencing using untreated and irradiated samples to assess the damage to the genome caused by radiation and understand the underlying mechanism.Results: The irradiated cancer cells exhibited hotspots for the de novo 8502–12966 single nucleotide variants and 954–1,331 indels on the chromosome. These single nucleotide variants primarily originated from double-stranded break repair errors, as determined using mutation signature analysis. The hotspots partially overlapped with the sites of H3K9 trimethylation, which are regions characterised by a weak capacity for double-stranded break repair.Conclusion: This study highlights the signature and underlying mechanism of radiation on the cancer genome. Irradiation Radiotherapy Whole genome sequence Cancer genome Mutation signature Mutation hot spot Du, Junyan verfasserin (orcid)0000-0003-1440-5538 aut Kaneko, Syuzo verfasserin aut Hamamoto, Ryuji verfasserin (orcid)0000-0002-2632-1334 aut Yamaguchi, Shigeo verfasserin aut Yamashita, Riu verfasserin aut Okumura, Masayuki verfasserin (orcid)0000-0002-9842-165X aut Motegi, Atsushi verfasserin aut Hojo, Hidehiro verfasserin aut Nakamura, Masaki verfasserin (orcid)0000-0002-4431-8892 aut Tsuchihara, Katsuya verfasserin aut Akimoto, Tetsuo verfasserin (orcid)0000-0003-0881-1345 aut Enthalten in Radiotherapy and oncology Amsterdam [u.a.] : Elsevier Science, 1983 155, Seite 10-16 Online-Ressource (DE-627)306710110 (DE-600)1500707-8 (DE-576)082435731 1879-0887 nnns volume:155 pages:10-16 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.81 Onkologie 44.64 Radiologie AR 155 10-16 |
| allfields_unstemmed |
10.1016/j.radonc.2020.10.020 doi (DE-627)ELV005692598 (ELSEVIER)S0167-8140(20)30856-2 DE-627 ger DE-627 rda eng 610 DE-600 44.81 bkl 44.64 bkl Kageyama, Shun-Ichiro verfasserin aut Identification of the mutation signature of the cancer genome caused by irradiation 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experimental system to analyse the presence of de novo mutations in the cancer genome of irradiated (60 Gy/20 fr/4 weeks) oesophageal cancer cell lines. Subsequently, we performed whole-genome, chromatin immunoprecipitation, and RNA sequencing using untreated and irradiated samples to assess the damage to the genome caused by radiation and understand the underlying mechanism.Results: The irradiated cancer cells exhibited hotspots for the de novo 8502–12966 single nucleotide variants and 954–1,331 indels on the chromosome. These single nucleotide variants primarily originated from double-stranded break repair errors, as determined using mutation signature analysis. The hotspots partially overlapped with the sites of H3K9 trimethylation, which are regions characterised by a weak capacity for double-stranded break repair.Conclusion: This study highlights the signature and underlying mechanism of radiation on the cancer genome. Irradiation Radiotherapy Whole genome sequence Cancer genome Mutation signature Mutation hot spot Du, Junyan verfasserin (orcid)0000-0003-1440-5538 aut Kaneko, Syuzo verfasserin aut Hamamoto, Ryuji verfasserin (orcid)0000-0002-2632-1334 aut Yamaguchi, Shigeo verfasserin aut Yamashita, Riu verfasserin aut Okumura, Masayuki verfasserin (orcid)0000-0002-9842-165X aut Motegi, Atsushi verfasserin aut Hojo, Hidehiro verfasserin aut Nakamura, Masaki verfasserin (orcid)0000-0002-4431-8892 aut Tsuchihara, Katsuya verfasserin aut Akimoto, Tetsuo verfasserin (orcid)0000-0003-0881-1345 aut Enthalten in Radiotherapy and oncology Amsterdam [u.a.] : Elsevier Science, 1983 155, Seite 10-16 Online-Ressource (DE-627)306710110 (DE-600)1500707-8 (DE-576)082435731 1879-0887 nnns volume:155 pages:10-16 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.81 Onkologie 44.64 Radiologie AR 155 10-16 |
| allfieldsGer |
10.1016/j.radonc.2020.10.020 doi (DE-627)ELV005692598 (ELSEVIER)S0167-8140(20)30856-2 DE-627 ger DE-627 rda eng 610 DE-600 44.81 bkl 44.64 bkl Kageyama, Shun-Ichiro verfasserin aut Identification of the mutation signature of the cancer genome caused by irradiation 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experimental system to analyse the presence of de novo mutations in the cancer genome of irradiated (60 Gy/20 fr/4 weeks) oesophageal cancer cell lines. Subsequently, we performed whole-genome, chromatin immunoprecipitation, and RNA sequencing using untreated and irradiated samples to assess the damage to the genome caused by radiation and understand the underlying mechanism.Results: The irradiated cancer cells exhibited hotspots for the de novo 8502–12966 single nucleotide variants and 954–1,331 indels on the chromosome. These single nucleotide variants primarily originated from double-stranded break repair errors, as determined using mutation signature analysis. The hotspots partially overlapped with the sites of H3K9 trimethylation, which are regions characterised by a weak capacity for double-stranded break repair.Conclusion: This study highlights the signature and underlying mechanism of radiation on the cancer genome. Irradiation Radiotherapy Whole genome sequence Cancer genome Mutation signature Mutation hot spot Du, Junyan verfasserin (orcid)0000-0003-1440-5538 aut Kaneko, Syuzo verfasserin aut Hamamoto, Ryuji verfasserin (orcid)0000-0002-2632-1334 aut Yamaguchi, Shigeo verfasserin aut Yamashita, Riu verfasserin aut Okumura, Masayuki verfasserin (orcid)0000-0002-9842-165X aut Motegi, Atsushi verfasserin aut Hojo, Hidehiro verfasserin aut Nakamura, Masaki verfasserin (orcid)0000-0002-4431-8892 aut Tsuchihara, Katsuya verfasserin aut Akimoto, Tetsuo verfasserin (orcid)0000-0003-0881-1345 aut Enthalten in Radiotherapy and oncology Amsterdam [u.a.] : Elsevier Science, 1983 155, Seite 10-16 Online-Ressource (DE-627)306710110 (DE-600)1500707-8 (DE-576)082435731 1879-0887 nnns volume:155 pages:10-16 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.81 Onkologie 44.64 Radiologie AR 155 10-16 |
| allfieldsSound |
10.1016/j.radonc.2020.10.020 doi (DE-627)ELV005692598 (ELSEVIER)S0167-8140(20)30856-2 DE-627 ger DE-627 rda eng 610 DE-600 44.81 bkl 44.64 bkl Kageyama, Shun-Ichiro verfasserin aut Identification of the mutation signature of the cancer genome caused by irradiation 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experimental system to analyse the presence of de novo mutations in the cancer genome of irradiated (60 Gy/20 fr/4 weeks) oesophageal cancer cell lines. Subsequently, we performed whole-genome, chromatin immunoprecipitation, and RNA sequencing using untreated and irradiated samples to assess the damage to the genome caused by radiation and understand the underlying mechanism.Results: The irradiated cancer cells exhibited hotspots for the de novo 8502–12966 single nucleotide variants and 954–1,331 indels on the chromosome. These single nucleotide variants primarily originated from double-stranded break repair errors, as determined using mutation signature analysis. The hotspots partially overlapped with the sites of H3K9 trimethylation, which are regions characterised by a weak capacity for double-stranded break repair.Conclusion: This study highlights the signature and underlying mechanism of radiation on the cancer genome. Irradiation Radiotherapy Whole genome sequence Cancer genome Mutation signature Mutation hot spot Du, Junyan verfasserin (orcid)0000-0003-1440-5538 aut Kaneko, Syuzo verfasserin aut Hamamoto, Ryuji verfasserin (orcid)0000-0002-2632-1334 aut Yamaguchi, Shigeo verfasserin aut Yamashita, Riu verfasserin aut Okumura, Masayuki verfasserin (orcid)0000-0002-9842-165X aut Motegi, Atsushi verfasserin aut Hojo, Hidehiro verfasserin aut Nakamura, Masaki verfasserin (orcid)0000-0002-4431-8892 aut Tsuchihara, Katsuya verfasserin aut Akimoto, Tetsuo verfasserin (orcid)0000-0003-0881-1345 aut Enthalten in Radiotherapy and oncology Amsterdam [u.a.] : Elsevier Science, 1983 155, Seite 10-16 Online-Ressource (DE-627)306710110 (DE-600)1500707-8 (DE-576)082435731 1879-0887 nnns volume:155 pages:10-16 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.81 Onkologie 44.64 Radiologie AR 155 10-16 |
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Enthalten in Radiotherapy and oncology 155, Seite 10-16 volume:155 pages:10-16 |
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Kageyama, Shun-Ichiro @@aut@@ Du, Junyan @@aut@@ Kaneko, Syuzo @@aut@@ Hamamoto, Ryuji @@aut@@ Yamaguchi, Shigeo @@aut@@ Yamashita, Riu @@aut@@ Okumura, Masayuki @@aut@@ Motegi, Atsushi @@aut@@ Hojo, Hidehiro @@aut@@ Nakamura, Masaki @@aut@@ Tsuchihara, Katsuya @@aut@@ Akimoto, Tetsuo @@aut@@ |
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Kageyama, Shun-Ichiro |
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610 DE-600 44.81 bkl 44.64 bkl Identification of the mutation signature of the cancer genome caused by irradiation Irradiation Radiotherapy Whole genome sequence Cancer genome Mutation signature Mutation hot spot |
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Identification of the mutation signature of the cancer genome caused by irradiation |
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Kageyama, Shun-Ichiro Du, Junyan Kaneko, Syuzo Hamamoto, Ryuji Yamaguchi, Shigeo Yamashita, Riu Okumura, Masayuki Motegi, Atsushi Hojo, Hidehiro Nakamura, Masaki Tsuchihara, Katsuya Akimoto, Tetsuo |
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identification of the mutation signature of the cancer genome caused by irradiation |
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Identification of the mutation signature of the cancer genome caused by irradiation |
| abstract |
Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experimental system to analyse the presence of de novo mutations in the cancer genome of irradiated (60 Gy/20 fr/4 weeks) oesophageal cancer cell lines. Subsequently, we performed whole-genome, chromatin immunoprecipitation, and RNA sequencing using untreated and irradiated samples to assess the damage to the genome caused by radiation and understand the underlying mechanism.Results: The irradiated cancer cells exhibited hotspots for the de novo 8502–12966 single nucleotide variants and 954–1,331 indels on the chromosome. These single nucleotide variants primarily originated from double-stranded break repair errors, as determined using mutation signature analysis. The hotspots partially overlapped with the sites of H3K9 trimethylation, which are regions characterised by a weak capacity for double-stranded break repair.Conclusion: This study highlights the signature and underlying mechanism of radiation on the cancer genome. |
| abstractGer |
Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experimental system to analyse the presence of de novo mutations in the cancer genome of irradiated (60 Gy/20 fr/4 weeks) oesophageal cancer cell lines. Subsequently, we performed whole-genome, chromatin immunoprecipitation, and RNA sequencing using untreated and irradiated samples to assess the damage to the genome caused by radiation and understand the underlying mechanism.Results: The irradiated cancer cells exhibited hotspots for the de novo 8502–12966 single nucleotide variants and 954–1,331 indels on the chromosome. These single nucleotide variants primarily originated from double-stranded break repair errors, as determined using mutation signature analysis. The hotspots partially overlapped with the sites of H3K9 trimethylation, which are regions characterised by a weak capacity for double-stranded break repair.Conclusion: This study highlights the signature and underlying mechanism of radiation on the cancer genome. |
| abstract_unstemmed |
Background and purpose: Ionising radiation causes mutations in the genomes of tumour cells and serves as a potent treatment for cancer. However, the mutation signatures in the cancer genome following ionising radiation have not been documented.Materials and methods: We established an in vitro experimental system to analyse the presence of de novo mutations in the cancer genome of irradiated (60 Gy/20 fr/4 weeks) oesophageal cancer cell lines. Subsequently, we performed whole-genome, chromatin immunoprecipitation, and RNA sequencing using untreated and irradiated samples to assess the damage to the genome caused by radiation and understand the underlying mechanism.Results: The irradiated cancer cells exhibited hotspots for the de novo 8502–12966 single nucleotide variants and 954–1,331 indels on the chromosome. These single nucleotide variants primarily originated from double-stranded break repair errors, as determined using mutation signature analysis. The hotspots partially overlapped with the sites of H3K9 trimethylation, which are regions characterised by a weak capacity for double-stranded break repair.Conclusion: This study highlights the signature and underlying mechanism of radiation on the cancer genome. |
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Identification of the mutation signature of the cancer genome caused by irradiation |
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Du, Junyan Kaneko, Syuzo Hamamoto, Ryuji Yamaguchi, Shigeo Yamashita, Riu Okumura, Masayuki Motegi, Atsushi Hojo, Hidehiro Nakamura, Masaki Tsuchihara, Katsuya Akimoto, Tetsuo |
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| score |
7.4018955 |