Functional analyses of chitinolytic enzymes in the formation of calcite prisms in

The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of a...
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Autor*in:	Kintsu, Hiroyuki [verfasserIn] Pérez-Huerta, Alberto [verfasserIn] Ohtsuka, Shigeru [verfasserIn] Okumura, Taiga [verfasserIn] Ifuku, Shinsuke [verfasserIn] Nagata, Koji [verfasserIn] Kogure, Toshihiro [verfasserIn] Suzuki, Michio [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Biomineralization Calcite Chitin Chitinolytic enzymes Prismatic layer

Übergeordnetes Werk:	Enthalten in: Micron - New York, NY [u.a.] : Elsevier, 1969, 145
Übergeordnetes Werk:	volume:145

DOI / URN:	10.1016/j.micron.2021.103063

Katalog-ID:	ELV005987687

Internformat


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520			\|a The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of aragonite tablets. The calcite crystal of prisms contains small angle grain boundaries caused by a dense intracrystalline organic matrix network to improve mechanical strength. Previously, we identified chitin and chitinolytic enzymes as components of this intracrystalline organic matrix. In this study, to reveal the function of those organic matrices in calcium carbonate crystallization, calcites synthesized in chitin gel with or without chitinolytic enzymes were analyzed by using transmission electron microscope (TEM) and atom probe tomography (APT), showing ion clusters derived from chitin inside of a calcite and small angle grain boundaries at optimal chitinolytic concentration. Furthermore, we performed the experiment in which chitinase inhibitor was injected into a living P. fucata. Nanoindentation and electron back scattered diffraction (EBSD) show that mechanical properties and crystal orientation were changed. These results suggested that chitinolytic enzymes work cooperatively with chitin to regulate the crystal growth and mechanical properties of the prismatic layer.
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650		4	\|a Calcite
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700	1		\|a Ifuku, Shinsuke \|e verfasserin \|4 aut
700	1		\|a Nagata, Koji \|e verfasserin \|4 aut
700	1		\|a Kogure, Toshihiro \|e verfasserin \|4 aut
700	1		\|a Suzuki, Michio \|e verfasserin \|0 (orcid)0000-0001-6150-8957 \|4 aut
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author_variant	h k hk a p h aph s o so t o to s i si k n kn t k tk m s ms
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publishDate	2021
allfields	10.1016/j.micron.2021.103063 doi (DE-627)ELV005987687 (ELSEVIER)S0968-4328(21)00054-8 DE-627 ger DE-627 rda eng 570 DE-600 BIODIV DE-30 fid 42.03 bkl Kintsu, Hiroyuki verfasserin (orcid)0000-0002-6462-3026 aut Functional analyses of chitinolytic enzymes in the formation of calcite prisms in 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of aragonite tablets. The calcite crystal of prisms contains small angle grain boundaries caused by a dense intracrystalline organic matrix network to improve mechanical strength. Previously, we identified chitin and chitinolytic enzymes as components of this intracrystalline organic matrix. In this study, to reveal the function of those organic matrices in calcium carbonate crystallization, calcites synthesized in chitin gel with or without chitinolytic enzymes were analyzed by using transmission electron microscope (TEM) and atom probe tomography (APT), showing ion clusters derived from chitin inside of a calcite and small angle grain boundaries at optimal chitinolytic concentration. Furthermore, we performed the experiment in which chitinase inhibitor was injected into a living P. fucata. Nanoindentation and electron back scattered diffraction (EBSD) show that mechanical properties and crystal orientation were changed. These results suggested that chitinolytic enzymes work cooperatively with chitin to regulate the crystal growth and mechanical properties of the prismatic layer. Biomineralization Calcite Chitin Chitinolytic enzymes Prismatic layer Pérez-Huerta, Alberto verfasserin aut Ohtsuka, Shigeru verfasserin aut Okumura, Taiga verfasserin aut Ifuku, Shinsuke verfasserin aut Nagata, Koji verfasserin aut Kogure, Toshihiro verfasserin aut Suzuki, Michio verfasserin (orcid)0000-0001-6150-8957 aut Enthalten in Micron New York, NY [u.a.] : Elsevier, 1969 145 Online-Ressource (DE-627)302720979 (DE-600)1492133-9 (DE-576)255266669 1878-4291 nnns volume:145 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.03 Methoden und Techniken der Biologie AR 145
spelling	10.1016/j.micron.2021.103063 doi (DE-627)ELV005987687 (ELSEVIER)S0968-4328(21)00054-8 DE-627 ger DE-627 rda eng 570 DE-600 BIODIV DE-30 fid 42.03 bkl Kintsu, Hiroyuki verfasserin (orcid)0000-0002-6462-3026 aut Functional analyses of chitinolytic enzymes in the formation of calcite prisms in 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of aragonite tablets. The calcite crystal of prisms contains small angle grain boundaries caused by a dense intracrystalline organic matrix network to improve mechanical strength. Previously, we identified chitin and chitinolytic enzymes as components of this intracrystalline organic matrix. In this study, to reveal the function of those organic matrices in calcium carbonate crystallization, calcites synthesized in chitin gel with or without chitinolytic enzymes were analyzed by using transmission electron microscope (TEM) and atom probe tomography (APT), showing ion clusters derived from chitin inside of a calcite and small angle grain boundaries at optimal chitinolytic concentration. Furthermore, we performed the experiment in which chitinase inhibitor was injected into a living P. fucata. Nanoindentation and electron back scattered diffraction (EBSD) show that mechanical properties and crystal orientation were changed. These results suggested that chitinolytic enzymes work cooperatively with chitin to regulate the crystal growth and mechanical properties of the prismatic layer. Biomineralization Calcite Chitin Chitinolytic enzymes Prismatic layer Pérez-Huerta, Alberto verfasserin aut Ohtsuka, Shigeru verfasserin aut Okumura, Taiga verfasserin aut Ifuku, Shinsuke verfasserin aut Nagata, Koji verfasserin aut Kogure, Toshihiro verfasserin aut Suzuki, Michio verfasserin (orcid)0000-0001-6150-8957 aut Enthalten in Micron New York, NY [u.a.] : Elsevier, 1969 145 Online-Ressource (DE-627)302720979 (DE-600)1492133-9 (DE-576)255266669 1878-4291 nnns volume:145 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.03 Methoden und Techniken der Biologie AR 145
allfields_unstemmed	10.1016/j.micron.2021.103063 doi (DE-627)ELV005987687 (ELSEVIER)S0968-4328(21)00054-8 DE-627 ger DE-627 rda eng 570 DE-600 BIODIV DE-30 fid 42.03 bkl Kintsu, Hiroyuki verfasserin (orcid)0000-0002-6462-3026 aut Functional analyses of chitinolytic enzymes in the formation of calcite prisms in 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of aragonite tablets. The calcite crystal of prisms contains small angle grain boundaries caused by a dense intracrystalline organic matrix network to improve mechanical strength. Previously, we identified chitin and chitinolytic enzymes as components of this intracrystalline organic matrix. In this study, to reveal the function of those organic matrices in calcium carbonate crystallization, calcites synthesized in chitin gel with or without chitinolytic enzymes were analyzed by using transmission electron microscope (TEM) and atom probe tomography (APT), showing ion clusters derived from chitin inside of a calcite and small angle grain boundaries at optimal chitinolytic concentration. Furthermore, we performed the experiment in which chitinase inhibitor was injected into a living P. fucata. Nanoindentation and electron back scattered diffraction (EBSD) show that mechanical properties and crystal orientation were changed. These results suggested that chitinolytic enzymes work cooperatively with chitin to regulate the crystal growth and mechanical properties of the prismatic layer. Biomineralization Calcite Chitin Chitinolytic enzymes Prismatic layer Pérez-Huerta, Alberto verfasserin aut Ohtsuka, Shigeru verfasserin aut Okumura, Taiga verfasserin aut Ifuku, Shinsuke verfasserin aut Nagata, Koji verfasserin aut Kogure, Toshihiro verfasserin aut Suzuki, Michio verfasserin (orcid)0000-0001-6150-8957 aut Enthalten in Micron New York, NY [u.a.] : Elsevier, 1969 145 Online-Ressource (DE-627)302720979 (DE-600)1492133-9 (DE-576)255266669 1878-4291 nnns volume:145 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.03 Methoden und Techniken der Biologie AR 145
allfieldsGer	10.1016/j.micron.2021.103063 doi (DE-627)ELV005987687 (ELSEVIER)S0968-4328(21)00054-8 DE-627 ger DE-627 rda eng 570 DE-600 BIODIV DE-30 fid 42.03 bkl Kintsu, Hiroyuki verfasserin (orcid)0000-0002-6462-3026 aut Functional analyses of chitinolytic enzymes in the formation of calcite prisms in 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of aragonite tablets. The calcite crystal of prisms contains small angle grain boundaries caused by a dense intracrystalline organic matrix network to improve mechanical strength. Previously, we identified chitin and chitinolytic enzymes as components of this intracrystalline organic matrix. In this study, to reveal the function of those organic matrices in calcium carbonate crystallization, calcites synthesized in chitin gel with or without chitinolytic enzymes were analyzed by using transmission electron microscope (TEM) and atom probe tomography (APT), showing ion clusters derived from chitin inside of a calcite and small angle grain boundaries at optimal chitinolytic concentration. Furthermore, we performed the experiment in which chitinase inhibitor was injected into a living P. fucata. Nanoindentation and electron back scattered diffraction (EBSD) show that mechanical properties and crystal orientation were changed. These results suggested that chitinolytic enzymes work cooperatively with chitin to regulate the crystal growth and mechanical properties of the prismatic layer. Biomineralization Calcite Chitin Chitinolytic enzymes Prismatic layer Pérez-Huerta, Alberto verfasserin aut Ohtsuka, Shigeru verfasserin aut Okumura, Taiga verfasserin aut Ifuku, Shinsuke verfasserin aut Nagata, Koji verfasserin aut Kogure, Toshihiro verfasserin aut Suzuki, Michio verfasserin (orcid)0000-0001-6150-8957 aut Enthalten in Micron New York, NY [u.a.] : Elsevier, 1969 145 Online-Ressource (DE-627)302720979 (DE-600)1492133-9 (DE-576)255266669 1878-4291 nnns volume:145 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.03 Methoden und Techniken der Biologie AR 145
allfieldsSound	10.1016/j.micron.2021.103063 doi (DE-627)ELV005987687 (ELSEVIER)S0968-4328(21)00054-8 DE-627 ger DE-627 rda eng 570 DE-600 BIODIV DE-30 fid 42.03 bkl Kintsu, Hiroyuki verfasserin (orcid)0000-0002-6462-3026 aut Functional analyses of chitinolytic enzymes in the formation of calcite prisms in 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of aragonite tablets. The calcite crystal of prisms contains small angle grain boundaries caused by a dense intracrystalline organic matrix network to improve mechanical strength. Previously, we identified chitin and chitinolytic enzymes as components of this intracrystalline organic matrix. In this study, to reveal the function of those organic matrices in calcium carbonate crystallization, calcites synthesized in chitin gel with or without chitinolytic enzymes were analyzed by using transmission electron microscope (TEM) and atom probe tomography (APT), showing ion clusters derived from chitin inside of a calcite and small angle grain boundaries at optimal chitinolytic concentration. Furthermore, we performed the experiment in which chitinase inhibitor was injected into a living P. fucata. Nanoindentation and electron back scattered diffraction (EBSD) show that mechanical properties and crystal orientation were changed. These results suggested that chitinolytic enzymes work cooperatively with chitin to regulate the crystal growth and mechanical properties of the prismatic layer. Biomineralization Calcite Chitin Chitinolytic enzymes Prismatic layer Pérez-Huerta, Alberto verfasserin aut Ohtsuka, Shigeru verfasserin aut Okumura, Taiga verfasserin aut Ifuku, Shinsuke verfasserin aut Nagata, Koji verfasserin aut Kogure, Toshihiro verfasserin aut Suzuki, Michio verfasserin (orcid)0000-0001-6150-8957 aut Enthalten in Micron New York, NY [u.a.] : Elsevier, 1969 145 Online-Ressource (DE-627)302720979 (DE-600)1492133-9 (DE-576)255266669 1878-4291 nnns volume:145 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.03 Methoden und Techniken der Biologie AR 145
language	English
source	Enthalten in Micron 145 volume:145
sourceStr	Enthalten in Micron 145 volume:145
format_phy_str_mv	Article
bklname	Methoden und Techniken der Biologie
institution	findex.gbv.de
topic_facet	Biomineralization Calcite Chitin Chitinolytic enzymes Prismatic layer
dewey-raw	570
isfreeaccess_bool	false
container_title	Micron
authorswithroles_txt_mv	Kintsu, Hiroyuki @@aut@@ Pérez-Huerta, Alberto @@aut@@ Ohtsuka, Shigeru @@aut@@ Okumura, Taiga @@aut@@ Ifuku, Shinsuke @@aut@@ Nagata, Koji @@aut@@ Kogure, Toshihiro @@aut@@ Suzuki, Michio @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	302720979
dewey-sort	3570
id	ELV005987687
language_de	englisch
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author	Kintsu, Hiroyuki
spellingShingle	Kintsu, Hiroyuki ddc 570 fid BIODIV bkl 42.03 misc Biomineralization misc Calcite misc Chitin misc Chitinolytic enzymes misc Prismatic layer Functional analyses of chitinolytic enzymes in the formation of calcite prisms in
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topic_title	570 DE-600 BIODIV DE-30 fid 42.03 bkl Functional analyses of chitinolytic enzymes in the formation of calcite prisms in Biomineralization Calcite Chitin Chitinolytic enzymes Prismatic layer
topic	ddc 570 fid BIODIV bkl 42.03 misc Biomineralization misc Calcite misc Chitin misc Chitinolytic enzymes misc Prismatic layer
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title	Functional analyses of chitinolytic enzymes in the formation of calcite prisms in
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title_full	Functional analyses of chitinolytic enzymes in the formation of calcite prisms in
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author_browse	Kintsu, Hiroyuki Pérez-Huerta, Alberto Ohtsuka, Shigeru Okumura, Taiga Ifuku, Shinsuke Nagata, Koji Kogure, Toshihiro Suzuki, Michio
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title_sort	functional analyses of chitinolytic enzymes in the formation of calcite prisms in
title_auth	Functional analyses of chitinolytic enzymes in the formation of calcite prisms in
abstract	The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of aragonite tablets. The calcite crystal of prisms contains small angle grain boundaries caused by a dense intracrystalline organic matrix network to improve mechanical strength. Previously, we identified chitin and chitinolytic enzymes as components of this intracrystalline organic matrix. In this study, to reveal the function of those organic matrices in calcium carbonate crystallization, calcites synthesized in chitin gel with or without chitinolytic enzymes were analyzed by using transmission electron microscope (TEM) and atom probe tomography (APT), showing ion clusters derived from chitin inside of a calcite and small angle grain boundaries at optimal chitinolytic concentration. Furthermore, we performed the experiment in which chitinase inhibitor was injected into a living P. fucata. Nanoindentation and electron back scattered diffraction (EBSD) show that mechanical properties and crystal orientation were changed. These results suggested that chitinolytic enzymes work cooperatively with chitin to regulate the crystal growth and mechanical properties of the prismatic layer.
abstractGer	The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of aragonite tablets. The calcite crystal of prisms contains small angle grain boundaries caused by a dense intracrystalline organic matrix network to improve mechanical strength. Previously, we identified chitin and chitinolytic enzymes as components of this intracrystalline organic matrix. In this study, to reveal the function of those organic matrices in calcium carbonate crystallization, calcites synthesized in chitin gel with or without chitinolytic enzymes were analyzed by using transmission electron microscope (TEM) and atom probe tomography (APT), showing ion clusters derived from chitin inside of a calcite and small angle grain boundaries at optimal chitinolytic concentration. Furthermore, we performed the experiment in which chitinase inhibitor was injected into a living P. fucata. Nanoindentation and electron back scattered diffraction (EBSD) show that mechanical properties and crystal orientation were changed. These results suggested that chitinolytic enzymes work cooperatively with chitin to regulate the crystal growth and mechanical properties of the prismatic layer.
abstract_unstemmed	The mollusk shells present distinctive microstructures that are formed by small amounts of organic matrices controlling the crystal growth of calcium carbonate. The shell of Pinctada fucata has the prismatic layer consisting of prisms of single calcite crystals and the nacreous layer consisting of aragonite tablets. The calcite crystal of prisms contains small angle grain boundaries caused by a dense intracrystalline organic matrix network to improve mechanical strength. Previously, we identified chitin and chitinolytic enzymes as components of this intracrystalline organic matrix. In this study, to reveal the function of those organic matrices in calcium carbonate crystallization, calcites synthesized in chitin gel with or without chitinolytic enzymes were analyzed by using transmission electron microscope (TEM) and atom probe tomography (APT), showing ion clusters derived from chitin inside of a calcite and small angle grain boundaries at optimal chitinolytic concentration. Furthermore, we performed the experiment in which chitinase inhibitor was injected into a living P. fucata. Nanoindentation and electron back scattered diffraction (EBSD) show that mechanical properties and crystal orientation were changed. These results suggested that chitinolytic enzymes work cooperatively with chitin to regulate the crystal growth and mechanical properties of the prismatic layer.
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title_short	Functional analyses of chitinolytic enzymes in the formation of calcite prisms in
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author2	Pérez-Huerta, Alberto Ohtsuka, Shigeru Okumura, Taiga Ifuku, Shinsuke Nagata, Koji Kogure, Toshihiro Suzuki, Michio
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up_date	2024-07-06T19:50:18.400Z
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Functional analyses of chitinolytic enzymes in the formation of calcite prisms in

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