The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019
Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene g...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Fongaro, Gislaine [verfasserIn] Stoco, Patrícia Hermes [verfasserIn] Souza, Doris Sobral Marques [verfasserIn] Grisard, Edmundo Carlos [verfasserIn] Magri, Maria Elisa [verfasserIn] Rogovski, Paula [verfasserIn] Schörner, Marcos André [verfasserIn] Barazzetti, Fernando Hartmann [verfasserIn] Christoff, Ana Paula [verfasserIn] de Oliveira, Luiz Felipe Valter [verfasserIn] Bazzo, Maria Luiza [verfasserIn] Wagner, Glauber [verfasserIn] Hernández, Marta [verfasserIn] Rodríguez-Lázaro, David [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2021 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: The science of the total environment - Amsterdam [u.a.] : Elsevier Science, 1972, 778 |
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| Übergeordnetes Werk: |
volume:778 |
| DOI / URN: |
10.1016/j.scitotenv.2021.146198 |
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| Katalog-ID: |
ELV005989965 |
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| 520 | |a Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L−1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L−1, ranging from 5.49 ± 0.02 log10 GC L−1 (27th November 2019) to 6.68 ± 0.02 log10 GC L−1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). | ||
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| 700 | 1 | |a Souza, Doris Sobral Marques |e verfasserin |4 aut | |
| 700 | 1 | |a Grisard, Edmundo Carlos |e verfasserin |4 aut | |
| 700 | 1 | |a Magri, Maria Elisa |e verfasserin |4 aut | |
| 700 | 1 | |a Rogovski, Paula |e verfasserin |4 aut | |
| 700 | 1 | |a Schörner, Marcos André |e verfasserin |4 aut | |
| 700 | 1 | |a Barazzetti, Fernando Hartmann |e verfasserin |4 aut | |
| 700 | 1 | |a Christoff, Ana Paula |e verfasserin |4 aut | |
| 700 | 1 | |a de Oliveira, Luiz Felipe Valter |e verfasserin |4 aut | |
| 700 | 1 | |a Bazzo, Maria Luiza |e verfasserin |4 aut | |
| 700 | 1 | |a Wagner, Glauber |e verfasserin |4 aut | |
| 700 | 1 | |a Hernández, Marta |e verfasserin |4 aut | |
| 700 | 1 | |a Rodríguez-Lázaro, David |e verfasserin |4 aut | |
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10.1016/j.scitotenv.2021.146198 doi (DE-627)ELV005989965 (ELSEVIER)S0048-9697(21)01265-1 DE-627 ger DE-627 rda eng 333.7 610 DE-600 43.12 bkl 43.13 bkl 44.13 bkl Fongaro, Gislaine verfasserin aut The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L−1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L−1, ranging from 5.49 ± 0.02 log10 GC L−1 (27th November 2019) to 6.68 ± 0.02 log10 GC L−1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). SARS-CoV-2 COVID-19 Human sewage Brazil Epidemiology Surveillance Stoco, Patrícia Hermes verfasserin aut Souza, Doris Sobral Marques verfasserin aut Grisard, Edmundo Carlos verfasserin aut Magri, Maria Elisa verfasserin aut Rogovski, Paula verfasserin aut Schörner, Marcos André verfasserin aut Barazzetti, Fernando Hartmann verfasserin aut Christoff, Ana Paula verfasserin aut de Oliveira, Luiz Felipe Valter verfasserin aut Bazzo, Maria Luiza verfasserin aut Wagner, Glauber verfasserin aut Hernández, Marta verfasserin aut Rodríguez-Lázaro, David verfasserin aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 778 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:778 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA SSG-OPC-GGO GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 43.12 Umweltchemie 43.13 Umwelttoxikologie 44.13 Medizinische Ökologie AR 778 |
| spelling |
10.1016/j.scitotenv.2021.146198 doi (DE-627)ELV005989965 (ELSEVIER)S0048-9697(21)01265-1 DE-627 ger DE-627 rda eng 333.7 610 DE-600 43.12 bkl 43.13 bkl 44.13 bkl Fongaro, Gislaine verfasserin aut The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L−1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L−1, ranging from 5.49 ± 0.02 log10 GC L−1 (27th November 2019) to 6.68 ± 0.02 log10 GC L−1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). SARS-CoV-2 COVID-19 Human sewage Brazil Epidemiology Surveillance Stoco, Patrícia Hermes verfasserin aut Souza, Doris Sobral Marques verfasserin aut Grisard, Edmundo Carlos verfasserin aut Magri, Maria Elisa verfasserin aut Rogovski, Paula verfasserin aut Schörner, Marcos André verfasserin aut Barazzetti, Fernando Hartmann verfasserin aut Christoff, Ana Paula verfasserin aut de Oliveira, Luiz Felipe Valter verfasserin aut Bazzo, Maria Luiza verfasserin aut Wagner, Glauber verfasserin aut Hernández, Marta verfasserin aut Rodríguez-Lázaro, David verfasserin aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 778 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:778 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA SSG-OPC-GGO GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 43.12 Umweltchemie 43.13 Umwelttoxikologie 44.13 Medizinische Ökologie AR 778 |
| allfields_unstemmed |
10.1016/j.scitotenv.2021.146198 doi (DE-627)ELV005989965 (ELSEVIER)S0048-9697(21)01265-1 DE-627 ger DE-627 rda eng 333.7 610 DE-600 43.12 bkl 43.13 bkl 44.13 bkl Fongaro, Gislaine verfasserin aut The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L−1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L−1, ranging from 5.49 ± 0.02 log10 GC L−1 (27th November 2019) to 6.68 ± 0.02 log10 GC L−1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). SARS-CoV-2 COVID-19 Human sewage Brazil Epidemiology Surveillance Stoco, Patrícia Hermes verfasserin aut Souza, Doris Sobral Marques verfasserin aut Grisard, Edmundo Carlos verfasserin aut Magri, Maria Elisa verfasserin aut Rogovski, Paula verfasserin aut Schörner, Marcos André verfasserin aut Barazzetti, Fernando Hartmann verfasserin aut Christoff, Ana Paula verfasserin aut de Oliveira, Luiz Felipe Valter verfasserin aut Bazzo, Maria Luiza verfasserin aut Wagner, Glauber verfasserin aut Hernández, Marta verfasserin aut Rodríguez-Lázaro, David verfasserin aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 778 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:778 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA SSG-OPC-GGO GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 43.12 Umweltchemie 43.13 Umwelttoxikologie 44.13 Medizinische Ökologie AR 778 |
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10.1016/j.scitotenv.2021.146198 doi (DE-627)ELV005989965 (ELSEVIER)S0048-9697(21)01265-1 DE-627 ger DE-627 rda eng 333.7 610 DE-600 43.12 bkl 43.13 bkl 44.13 bkl Fongaro, Gislaine verfasserin aut The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L−1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L−1, ranging from 5.49 ± 0.02 log10 GC L−1 (27th November 2019) to 6.68 ± 0.02 log10 GC L−1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). SARS-CoV-2 COVID-19 Human sewage Brazil Epidemiology Surveillance Stoco, Patrícia Hermes verfasserin aut Souza, Doris Sobral Marques verfasserin aut Grisard, Edmundo Carlos verfasserin aut Magri, Maria Elisa verfasserin aut Rogovski, Paula verfasserin aut Schörner, Marcos André verfasserin aut Barazzetti, Fernando Hartmann verfasserin aut Christoff, Ana Paula verfasserin aut de Oliveira, Luiz Felipe Valter verfasserin aut Bazzo, Maria Luiza verfasserin aut Wagner, Glauber verfasserin aut Hernández, Marta verfasserin aut Rodríguez-Lázaro, David verfasserin aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 778 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:778 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA SSG-OPC-GGO GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 43.12 Umweltchemie 43.13 Umwelttoxikologie 44.13 Medizinische Ökologie AR 778 |
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10.1016/j.scitotenv.2021.146198 doi (DE-627)ELV005989965 (ELSEVIER)S0048-9697(21)01265-1 DE-627 ger DE-627 rda eng 333.7 610 DE-600 43.12 bkl 43.13 bkl 44.13 bkl Fongaro, Gislaine verfasserin aut The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L−1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L−1, ranging from 5.49 ± 0.02 log10 GC L−1 (27th November 2019) to 6.68 ± 0.02 log10 GC L−1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). SARS-CoV-2 COVID-19 Human sewage Brazil Epidemiology Surveillance Stoco, Patrícia Hermes verfasserin aut Souza, Doris Sobral Marques verfasserin aut Grisard, Edmundo Carlos verfasserin aut Magri, Maria Elisa verfasserin aut Rogovski, Paula verfasserin aut Schörner, Marcos André verfasserin aut Barazzetti, Fernando Hartmann verfasserin aut Christoff, Ana Paula verfasserin aut de Oliveira, Luiz Felipe Valter verfasserin aut Bazzo, Maria Luiza verfasserin aut Wagner, Glauber verfasserin aut Hernández, Marta verfasserin aut Rodríguez-Lázaro, David verfasserin aut Enthalten in The science of the total environment Amsterdam [u.a.] : Elsevier Science, 1972 778 Online-Ressource (DE-627)306591456 (DE-600)1498726-0 (DE-576)081953178 1879-1026 nnns volume:778 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA SSG-OPC-GGO GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 43.12 Umweltchemie 43.13 Umwelttoxikologie 44.13 Medizinische Ökologie AR 778 |
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Fongaro, Gislaine @@aut@@ Stoco, Patrícia Hermes @@aut@@ Souza, Doris Sobral Marques @@aut@@ Grisard, Edmundo Carlos @@aut@@ Magri, Maria Elisa @@aut@@ Rogovski, Paula @@aut@@ Schörner, Marcos André @@aut@@ Barazzetti, Fernando Hartmann @@aut@@ Christoff, Ana Paula @@aut@@ de Oliveira, Luiz Felipe Valter @@aut@@ Bazzo, Maria Luiza @@aut@@ Wagner, Glauber @@aut@@ Hernández, Marta @@aut@@ Rodríguez-Lázaro, David @@aut@@ |
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Fongaro, Gislaine |
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Fongaro, Gislaine ddc 333.7 bkl 43.12 bkl 43.13 bkl 44.13 misc SARS-CoV-2 misc COVID-19 misc Human sewage misc Brazil misc Epidemiology misc Surveillance The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 |
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333.7 610 DE-600 43.12 bkl 43.13 bkl 44.13 bkl The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 SARS-CoV-2 COVID-19 Human sewage Brazil Epidemiology Surveillance |
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Fongaro, Gislaine Stoco, Patrícia Hermes Souza, Doris Sobral Marques Grisard, Edmundo Carlos Magri, Maria Elisa Rogovski, Paula Schörner, Marcos André Barazzetti, Fernando Hartmann Christoff, Ana Paula de Oliveira, Luiz Felipe Valter Bazzo, Maria Luiza Wagner, Glauber Hernández, Marta Rodríguez-Lázaro, David |
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the presence of sars-cov-2 rna in human sewage in santa catarina, brazil, november 2019 |
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The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 |
| abstract |
Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L−1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L−1, ranging from 5.49 ± 0.02 log10 GC L−1 (27th November 2019) to 6.68 ± 0.02 log10 GC L−1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). |
| abstractGer |
Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L−1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L−1, ranging from 5.49 ± 0.02 log10 GC L−1 (27th November 2019) to 6.68 ± 0.02 log10 GC L−1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). |
| abstract_unstemmed |
Human sewage from Florianopolis (Santa Catarina, Brazil) was analyzed for severe acute respiratory syndrome coronavirus-2 (SARS-CoV2) from October 2019 until March 2020. Twenty five ml of sewage samples were clarified and viruses concentrated using a glycine buffer method coupled with polyethylene glycol precipitation, and viral RNA extracted using a commercial kit. SARS-CoV-2 RNA was detected by RT-qPCR using oligonucleotides targeting N1, S and two RdRp regions. The results of all positive samples were further confirmed by a different RT-qPCR system in an independent laboratory. S and RdRp amplicons were sequenced to confirm identity with SARS-CoV-2. Genome sequencing was performed using two strategies; a sequence-independent single-primer amplification (SISPA) approach, and by direct metagenomics using Illumina's NGS. SARS-CoV-2 RNA was detected on 27th November 2019 (5.49 ± 0.02 log10 SARS-CoV-2 genome copies (GC) L−1), detection being confirmed by an independent laboratory and genome sequencing analysis. The samples in the subsequent three events were positive by all RT-qPCR assays; these positive results were also confirmed by an independent laboratory. The average load was 5.83 ± 0.12 log10 SARS-CoV-2 GC L−1, ranging from 5.49 ± 0.02 log10 GC L−1 (27th November 2019) to 6.68 ± 0.02 log10 GC L−1 (4th March 2020). Our findings demonstrate that SARS-CoV-2 was likely circulating undetected in the community in Brazil since November 2019, earlier than the first reported case in the Americas (21st January 2020). |
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The presence of SARS-CoV-2 RNA in human sewage in Santa Catarina, Brazil, November 2019 |
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Stoco, Patrícia Hermes Souza, Doris Sobral Marques Grisard, Edmundo Carlos Magri, Maria Elisa Rogovski, Paula Schörner, Marcos André Barazzetti, Fernando Hartmann Christoff, Ana Paula de Oliveira, Luiz Felipe Valter Bazzo, Maria Luiza Wagner, Glauber Hernández, Marta Rodríguez-Lázaro, David |
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| score |
7.3996897 |