TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells

Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Yan, Ying [verfasserIn] Liu, Feng [verfasserIn] Dang, Xitong [verfasserIn] Zhou, Rui [verfasserIn] Liao, Bin [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Cardiomyocytes Human induced pluripotent stem cells (hiPSC) T-box transcription factor 3 (TBX3) Sinoatrial node like cells (SANLC)

Übergeordnetes Werk:	Enthalten in: Gene expression patterns - Amsterdam [u.a.] : Elsevier, 2002, 40
Übergeordnetes Werk:	volume:40

DOI / URN:	10.1016/j.gep.2021.119184

Katalog-ID:	ELV006137598

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245	1	0	\|a TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells
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520			\|a Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC)，TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SAN-specific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5.Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells.
650		4	\|a Cardiomyocytes
650		4	\|a Human induced pluripotent stem cells (hiPSC)
650		4	\|a T-box transcription factor 3 (TBX3)
650		4	\|a Sinoatrial node like cells (SANLC)
700	1		\|a Liu, Feng \|e verfasserin \|4 aut
700	1		\|a Dang, Xitong \|e verfasserin \|4 aut
700	1		\|a Zhou, Rui \|e verfasserin \|4 aut
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912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2003
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936	b	k	\|a 42.23 \|j Entwicklungsbiologie
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952			\|d 40

Indexfelder

author_variant	y y yy f l fl x d xd r z rz b l bl
matchkey_str	article:18727298:2021----::b3nuebaedfeetainfuaidcdlrptnseclsn
hierarchy_sort_str	2021
bklnumber	42.23
publishDate	2021
allfields	10.1016/j.gep.2021.119184 doi (DE-627)ELV006137598 (ELSEVIER)S1567-133X(21)00019-3 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Yan, Ying verfasserin aut TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC)，TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SAN-specific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5.Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells. Cardiomyocytes Human induced pluripotent stem cells (hiPSC) T-box transcription factor 3 (TBX3) Sinoatrial node like cells (SANLC) Liu, Feng verfasserin aut Dang, Xitong verfasserin aut Zhou, Rui verfasserin aut Liao, Bin verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 40 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:40 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 40
spelling	10.1016/j.gep.2021.119184 doi (DE-627)ELV006137598 (ELSEVIER)S1567-133X(21)00019-3 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Yan, Ying verfasserin aut TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC)，TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SAN-specific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5.Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells. Cardiomyocytes Human induced pluripotent stem cells (hiPSC) T-box transcription factor 3 (TBX3) Sinoatrial node like cells (SANLC) Liu, Feng verfasserin aut Dang, Xitong verfasserin aut Zhou, Rui verfasserin aut Liao, Bin verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 40 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:40 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 40
allfields_unstemmed	10.1016/j.gep.2021.119184 doi (DE-627)ELV006137598 (ELSEVIER)S1567-133X(21)00019-3 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Yan, Ying verfasserin aut TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC)，TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SAN-specific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5.Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells. Cardiomyocytes Human induced pluripotent stem cells (hiPSC) T-box transcription factor 3 (TBX3) Sinoatrial node like cells (SANLC) Liu, Feng verfasserin aut Dang, Xitong verfasserin aut Zhou, Rui verfasserin aut Liao, Bin verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 40 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:40 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 40
allfieldsGer	10.1016/j.gep.2021.119184 doi (DE-627)ELV006137598 (ELSEVIER)S1567-133X(21)00019-3 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Yan, Ying verfasserin aut TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC)，TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SAN-specific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5.Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells. Cardiomyocytes Human induced pluripotent stem cells (hiPSC) T-box transcription factor 3 (TBX3) Sinoatrial node like cells (SANLC) Liu, Feng verfasserin aut Dang, Xitong verfasserin aut Zhou, Rui verfasserin aut Liao, Bin verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 40 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:40 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 40
allfieldsSound	10.1016/j.gep.2021.119184 doi (DE-627)ELV006137598 (ELSEVIER)S1567-133X(21)00019-3 DE-627 ger DE-627 rda eng 570 610 DE-600 42.23 bkl Yan, Ying verfasserin aut TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC)，TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SAN-specific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5.Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells. Cardiomyocytes Human induced pluripotent stem cells (hiPSC) T-box transcription factor 3 (TBX3) Sinoatrial node like cells (SANLC) Liu, Feng verfasserin aut Dang, Xitong verfasserin aut Zhou, Rui verfasserin aut Liao, Bin verfasserin aut Enthalten in Gene expression patterns Amsterdam [u.a.] : Elsevier, 2002 40 Online-Ressource (DE-627)34007521X (DE-600)2065045-0 (DE-576)099604140 1872-7298 nnns volume:40 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 42.23 Entwicklungsbiologie AR 40
language	English
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topic_facet	Cardiomyocytes Human induced pluripotent stem cells (hiPSC) T-box transcription factor 3 (TBX3) Sinoatrial node like cells (SANLC)
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authorswithroles_txt_mv	Yan, Ying @@aut@@ Liu, Feng @@aut@@ Dang, Xitong @@aut@@ Zhou, Rui @@aut@@ Liao, Bin @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
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author	Yan, Ying
spellingShingle	Yan, Ying ddc 570 bkl 42.23 misc Cardiomyocytes misc Human induced pluripotent stem cells (hiPSC) misc T-box transcription factor 3 (TBX3) misc Sinoatrial node like cells (SANLC) TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells
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topic_title	570 610 DE-600 42.23 bkl TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells Cardiomyocytes Human induced pluripotent stem cells (hiPSC) T-box transcription factor 3 (TBX3) Sinoatrial node like cells (SANLC)
topic	ddc 570 bkl 42.23 misc Cardiomyocytes misc Human induced pluripotent stem cells (hiPSC) misc T-box transcription factor 3 (TBX3) misc Sinoatrial node like cells (SANLC)
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title	TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells
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title_full	TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells
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title_sort	tbx3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells
title_auth	TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells
abstract	Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC)，TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SAN-specific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5.Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells.
abstractGer	Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC)，TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SAN-specific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5.Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells.
abstract_unstemmed	Background: TBX3 plays a critical role in the formation of the sinoatrial node (SAN) during embryonic heart development. However, the contribution of TBX3 in driving the differentiation of human induced pluripotent stem cells (hiPSC)into pacemaker cells remains to be explored.Results: Using the pan-cardiomyocyte differentiation protocol of human induced pluripotent stem cells (hiPSC)，TBX3 gene was introduced into the differentiating hiPSC on day 5 post-differentiation, and the differentiation of pacemaker-like cardiomyocytes was evaluated on day 21. The results showed that TBX3 significantly induced biased differentiation of hiPSC into pacemaker-like cells as judged by significantly increased expression of SAN-specific marker gene, SHOX2, and slightly decreased expression of SAN-detrimental transcription factor, NKX2-5.Conclusion: Our results suggest that TBX3 plays an important role in driving the differentiation of hiPSC into pacemaker-like cells, and manipulation of TBX3 expression during pan-cardiomyocyte differentiation may lead to the development of therapeutic pacemaker cells.
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title_short	TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells
remote_bool	true
author2	Liu, Feng Dang, Xitong Zhou, Rui Liao, Bin
author2Str	Liu, Feng Dang, Xitong Zhou, Rui Liao, Bin
ppnlink	34007521X
mediatype_str_mv	c
isOA_txt	false
hochschulschrift_bool	false
doi_str	10.1016/j.gep.2021.119184
up_date	2024-07-06T20:21:09.109Z
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TBX3 induces biased differentiation of human induced pluripotent stem cells into cardiac pacemaker-like cells

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