Polyamine elicited aristolochic acid production in in vitro clonally fidel
The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. ind...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Dey, Abhijit [verfasserIn] Nongdam, Potsangbam [verfasserIn] Nandy, Samapika [verfasserIn] Mukherjee, Sabyasachi [verfasserIn] Mukherjee, Anuradha [verfasserIn] Tikendra, Leimapokpam [verfasserIn] Hazra, Alok Kumar [verfasserIn] Pandey, Devendra Kumar [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2020 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: South African journal of botany - Amsterdam : Elsevier, 1982, 140, Seite 326-335 |
|---|---|
| Übergeordnetes Werk: |
volume:140 ; pages:326-335 |
| DOI / URN: |
10.1016/j.sajb.2020.06.018 |
|---|
| Katalog-ID: |
ELV006526438 |
|---|
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| 100 | 1 | |a Dey, Abhijit |e verfasserin |0 (orcid)0000-0002-5750-0802 |4 aut | |
| 245 | 1 | 0 | |a Polyamine elicited aristolochic acid production in in vitro clonally fidel |
| 264 | 1 | |c 2020 | |
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| 337 | |a Computermedien |b c |2 rdamedia | ||
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| 520 | |a The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively. | ||
| 650 | 4 | |a Micropropagation | |
| 650 | 4 | |a Clonal fidelity | |
| 650 | 4 | |a Polyamines | |
| 650 | 4 | |a HPTLC | |
| 650 | 4 | |a Aristolochic acid | |
| 650 | 4 | |a ISSR | |
| 650 | 4 | |a RAPD | |
| 700 | 1 | |a Nongdam, Potsangbam |e verfasserin |4 aut | |
| 700 | 1 | |a Nandy, Samapika |e verfasserin |0 (orcid)0000-0002-7681-7741 |4 aut | |
| 700 | 1 | |a Mukherjee, Sabyasachi |e verfasserin |4 aut | |
| 700 | 1 | |a Mukherjee, Anuradha |e verfasserin |4 aut | |
| 700 | 1 | |a Tikendra, Leimapokpam |e verfasserin |4 aut | |
| 700 | 1 | |a Hazra, Alok Kumar |e verfasserin |4 aut | |
| 700 | 1 | |a Pandey, Devendra Kumar |e verfasserin |0 (orcid)0000-0002-1582-9407 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t South African journal of botany |d Amsterdam : Elsevier, 1982 |g 140, Seite 326-335 |h Online-Ressource |w (DE-627)373504608 |w (DE-600)2126918-X |w (DE-576)259486205 |7 nnns |
| 773 | 1 | 8 | |g volume:140 |g pages:326-335 |
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| 912 | |a GBV_ILN_2020 | ||
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| 912 | |a GBV_ILN_2026 | ||
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| 912 | |a GBV_ILN_4251 | ||
| 912 | |a GBV_ILN_4305 | ||
| 912 | |a GBV_ILN_4306 | ||
| 912 | |a GBV_ILN_4307 | ||
| 912 | |a GBV_ILN_4313 | ||
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| 936 | b | k | |a 42.38 |j Botanik: Allgemeines |
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2020 |
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2020 |
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10.1016/j.sajb.2020.06.018 doi (DE-627)ELV006526438 (ELSEVIER)S0254-6299(20)30955-8 DE-627 ger DE-627 rda eng 580 DE-600 AFRIKA DE-30 fid BIODIV DE-30 fid 42.38 bkl Dey, Abhijit verfasserin (orcid)0000-0002-5750-0802 aut Polyamine elicited aristolochic acid production in in vitro clonally fidel 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively. Micropropagation Clonal fidelity Polyamines HPTLC Aristolochic acid ISSR RAPD Nongdam, Potsangbam verfasserin aut Nandy, Samapika verfasserin (orcid)0000-0002-7681-7741 aut Mukherjee, Sabyasachi verfasserin aut Mukherjee, Anuradha verfasserin aut Tikendra, Leimapokpam verfasserin aut Hazra, Alok Kumar verfasserin aut Pandey, Devendra Kumar verfasserin (orcid)0000-0002-1582-9407 aut Enthalten in South African journal of botany Amsterdam : Elsevier, 1982 140, Seite 326-335 Online-Ressource (DE-627)373504608 (DE-600)2126918-X (DE-576)259486205 nnns volume:140 pages:326-335 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-AFRIKA FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.38 Botanik: Allgemeines AR 140 326-335 |
| spelling |
10.1016/j.sajb.2020.06.018 doi (DE-627)ELV006526438 (ELSEVIER)S0254-6299(20)30955-8 DE-627 ger DE-627 rda eng 580 DE-600 AFRIKA DE-30 fid BIODIV DE-30 fid 42.38 bkl Dey, Abhijit verfasserin (orcid)0000-0002-5750-0802 aut Polyamine elicited aristolochic acid production in in vitro clonally fidel 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively. Micropropagation Clonal fidelity Polyamines HPTLC Aristolochic acid ISSR RAPD Nongdam, Potsangbam verfasserin aut Nandy, Samapika verfasserin (orcid)0000-0002-7681-7741 aut Mukherjee, Sabyasachi verfasserin aut Mukherjee, Anuradha verfasserin aut Tikendra, Leimapokpam verfasserin aut Hazra, Alok Kumar verfasserin aut Pandey, Devendra Kumar verfasserin (orcid)0000-0002-1582-9407 aut Enthalten in South African journal of botany Amsterdam : Elsevier, 1982 140, Seite 326-335 Online-Ressource (DE-627)373504608 (DE-600)2126918-X (DE-576)259486205 nnns volume:140 pages:326-335 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-AFRIKA FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.38 Botanik: Allgemeines AR 140 326-335 |
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10.1016/j.sajb.2020.06.018 doi (DE-627)ELV006526438 (ELSEVIER)S0254-6299(20)30955-8 DE-627 ger DE-627 rda eng 580 DE-600 AFRIKA DE-30 fid BIODIV DE-30 fid 42.38 bkl Dey, Abhijit verfasserin (orcid)0000-0002-5750-0802 aut Polyamine elicited aristolochic acid production in in vitro clonally fidel 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively. Micropropagation Clonal fidelity Polyamines HPTLC Aristolochic acid ISSR RAPD Nongdam, Potsangbam verfasserin aut Nandy, Samapika verfasserin (orcid)0000-0002-7681-7741 aut Mukherjee, Sabyasachi verfasserin aut Mukherjee, Anuradha verfasserin aut Tikendra, Leimapokpam verfasserin aut Hazra, Alok Kumar verfasserin aut Pandey, Devendra Kumar verfasserin (orcid)0000-0002-1582-9407 aut Enthalten in South African journal of botany Amsterdam : Elsevier, 1982 140, Seite 326-335 Online-Ressource (DE-627)373504608 (DE-600)2126918-X (DE-576)259486205 nnns volume:140 pages:326-335 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-AFRIKA FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.38 Botanik: Allgemeines AR 140 326-335 |
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10.1016/j.sajb.2020.06.018 doi (DE-627)ELV006526438 (ELSEVIER)S0254-6299(20)30955-8 DE-627 ger DE-627 rda eng 580 DE-600 AFRIKA DE-30 fid BIODIV DE-30 fid 42.38 bkl Dey, Abhijit verfasserin (orcid)0000-0002-5750-0802 aut Polyamine elicited aristolochic acid production in in vitro clonally fidel 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively. Micropropagation Clonal fidelity Polyamines HPTLC Aristolochic acid ISSR RAPD Nongdam, Potsangbam verfasserin aut Nandy, Samapika verfasserin (orcid)0000-0002-7681-7741 aut Mukherjee, Sabyasachi verfasserin aut Mukherjee, Anuradha verfasserin aut Tikendra, Leimapokpam verfasserin aut Hazra, Alok Kumar verfasserin aut Pandey, Devendra Kumar verfasserin (orcid)0000-0002-1582-9407 aut Enthalten in South African journal of botany Amsterdam : Elsevier, 1982 140, Seite 326-335 Online-Ressource (DE-627)373504608 (DE-600)2126918-X (DE-576)259486205 nnns volume:140 pages:326-335 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-AFRIKA FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.38 Botanik: Allgemeines AR 140 326-335 |
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10.1016/j.sajb.2020.06.018 doi (DE-627)ELV006526438 (ELSEVIER)S0254-6299(20)30955-8 DE-627 ger DE-627 rda eng 580 DE-600 AFRIKA DE-30 fid BIODIV DE-30 fid 42.38 bkl Dey, Abhijit verfasserin (orcid)0000-0002-5750-0802 aut Polyamine elicited aristolochic acid production in in vitro clonally fidel 2020 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively. Micropropagation Clonal fidelity Polyamines HPTLC Aristolochic acid ISSR RAPD Nongdam, Potsangbam verfasserin aut Nandy, Samapika verfasserin (orcid)0000-0002-7681-7741 aut Mukherjee, Sabyasachi verfasserin aut Mukherjee, Anuradha verfasserin aut Tikendra, Leimapokpam verfasserin aut Hazra, Alok Kumar verfasserin aut Pandey, Devendra Kumar verfasserin (orcid)0000-0002-1582-9407 aut Enthalten in South African journal of botany Amsterdam : Elsevier, 1982 140, Seite 326-335 Online-Ressource (DE-627)373504608 (DE-600)2126918-X (DE-576)259486205 nnns volume:140 pages:326-335 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-AFRIKA FID-BIODIV GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.38 Botanik: Allgemeines AR 140 326-335 |
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Dey, Abhijit |
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Dey, Abhijit ddc 580 fid AFRIKA fid BIODIV bkl 42.38 misc Micropropagation misc Clonal fidelity misc Polyamines misc HPTLC misc Aristolochic acid misc ISSR misc RAPD Polyamine elicited aristolochic acid production in in vitro clonally fidel |
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580 DE-600 AFRIKA DE-30 fid BIODIV DE-30 fid 42.38 bkl Polyamine elicited aristolochic acid production in in vitro clonally fidel Micropropagation Clonal fidelity Polyamines HPTLC Aristolochic acid ISSR RAPD |
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ddc 580 fid AFRIKA fid BIODIV bkl 42.38 misc Micropropagation misc Clonal fidelity misc Polyamines misc HPTLC misc Aristolochic acid misc ISSR misc RAPD |
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ddc 580 fid AFRIKA fid BIODIV bkl 42.38 misc Micropropagation misc Clonal fidelity misc Polyamines misc HPTLC misc Aristolochic acid misc ISSR misc RAPD |
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Polyamine elicited aristolochic acid production in in vitro clonally fidel |
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Polyamine elicited aristolochic acid production in in vitro clonally fidel |
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Dey, Abhijit Nongdam, Potsangbam Nandy, Samapika Mukherjee, Sabyasachi Mukherjee, Anuradha Tikendra, Leimapokpam Hazra, Alok Kumar Pandey, Devendra Kumar |
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polyamine elicited aristolochic acid production in in vitro clonally fidel |
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Polyamine elicited aristolochic acid production in in vitro clonally fidel |
| abstract |
The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively. |
| abstractGer |
The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively. |
| abstract_unstemmed |
The present investigation was conducted to find out the influence of different polyamines (PAs) viz. spermine (spm), spermidine (spd) and putrescine (put) and plant growth regulators (PGRs) on multiplication, rooting, callusing and regeneration in Aristolochia indica L. in vitro. Firstly, the A. indica plants were collected, surface sterilized and cultured in Schenk and Hildebrandt (SH) media fortified with various PGRs and PAs in different concentrations and combinations. SH media fortified with benzylaminopurine (BAP) (2.0 mg/l)+put (0.5 mM) and in another experiment, BAP (2.0 mg/l)+spd (1 mM) produced best results with 41.00 and 39.2 axillary shoots/ nodal explant respectively. Following 4-6 weeks of incubation, explants propagated in SH media augmented with various combinations and concentrations of BAP and kinetin (kin) (0.5, 1, 1.5 and 2 mg/l), spm, spd and put (0.5 and 1 mM) generated white friable callus. SH media supplemented with BAP (2.0 mg/l)+spd (0.5 mM) displayed best response with an average number of 47.5 base callus derived shoots . Following 6 weeks of incubation, SH+ indole-3-acetic acid (IAA) (1.0 mg/l)+spd (0.5 mM) resulted maximum average number (7) of roots per shoot. The genetic homogeneity of the mother plant (MP), hardened plant (HP) and in vitro regenerated plants was ascertained employing random-amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. In this present study, in randomly selected A. indica plants, by using 6 ISSR and 5 RAPD primers, 63 scorable bands were derived, 59 of which were recorded as monomorphic with a degree of monomorphism of 90%. High-performance thin layer chromatography (HPTLC) revealed aristolochic acid (AA) content in in vitro grown A. indica roots and the roots from the mother plant (MP) as 6074.54 µg/g and 5891.14 µg/g respectively. |
| collection_details |
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| title_short |
Polyamine elicited aristolochic acid production in in vitro clonally fidel |
| remote_bool |
true |
| author2 |
Nongdam, Potsangbam Nandy, Samapika Mukherjee, Sabyasachi Mukherjee, Anuradha Tikendra, Leimapokpam Hazra, Alok Kumar Pandey, Devendra Kumar |
| author2Str |
Nongdam, Potsangbam Nandy, Samapika Mukherjee, Sabyasachi Mukherjee, Anuradha Tikendra, Leimapokpam Hazra, Alok Kumar Pandey, Devendra Kumar |
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373504608 |
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c |
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false |
| hochschulschrift_bool |
false |
| doi_str |
10.1016/j.sajb.2020.06.018 |
| up_date |
2024-07-06T21:40:56.019Z |
| _version_ |
1803867448694276096 |
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| score |
7.400218 |