Production of (−)-α-bisabolol in metabolically engineered

(−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The...
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Gespeichert in:

Autor*in:	Kim, Tae Yeob [verfasserIn] Park, Haeseong [verfasserIn] Kim, Sun-Ki [verfasserIn] Kim, Soo-Jung [verfasserIn] Park, Yong-Cheol [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	(−)-α-Bisabolol

Übergeordnetes Werk:	Enthalten in: Journal of biotechnology - Amsterdam [u.a.] : Elsevier Science, 1984, 340, Seite 13-21
Übergeordnetes Werk:	volume:340 ; pages:13-21

DOI / URN:	10.1016/j.jbiotec.2021.08.008

Katalog-ID:	ELV006721796

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520			\|a (−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The codon-optimized MrBBS gene coding for (−)-α-bisabolol synthase from Matricaria recutita was expressed in S. cerevisiae for (−)-α-bisabolol production. The resulting strain (DM) produced 9.5 mg/L of (−)-α-bisabolol in 24 h of batch culture. Additionally, the mevalonate pathway was intensified by introducing a truncated HMG1 gene coding for HMG-CoA reductase and ERG10 encoding acetyl-CoA thiolase. The resulting strain (DtEM) produced a 2.9-fold increased concentration of (−)-α-bisabolol than the DM strain. To increase the acetyl-CoA pool, the ACS1 gene coding for acetyl-CoA synthetase was also overexpressed in the DtEM strain. Finally, the DtEMA strain produced 124 mg/L of (−)-α-bisabolol with 2.7 mg/L-h of productivity in a fed-batch fermentation, which were 13 and 6.8 times higher than the DM strain in batch culture, respectively. Conclusively, these metabolically-engineered approaches might pave the way for the sustainable production of other sesquiterpenes in engineered S. cerevisiae.
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allfields	10.1016/j.jbiotec.2021.08.008 doi (DE-627)ELV006721796 (ELSEVIER)S0168-1656(21)00219-4 DE-627 ger DE-627 rda eng 540 DE-600 58.30 bkl Kim, Tae Yeob verfasserin aut Production of (−)-α-bisabolol in metabolically engineered 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The codon-optimized MrBBS gene coding for (−)-α-bisabolol synthase from Matricaria recutita was expressed in S. cerevisiae for (−)-α-bisabolol production. The resulting strain (DM) produced 9.5 mg/L of (−)-α-bisabolol in 24 h of batch culture. Additionally, the mevalonate pathway was intensified by introducing a truncated HMG1 gene coding for HMG-CoA reductase and ERG10 encoding acetyl-CoA thiolase. The resulting strain (DtEM) produced a 2.9-fold increased concentration of (−)-α-bisabolol than the DM strain. To increase the acetyl-CoA pool, the ACS1 gene coding for acetyl-CoA synthetase was also overexpressed in the DtEM strain. Finally, the DtEMA strain produced 124 mg/L of (−)-α-bisabolol with 2.7 mg/L-h of productivity in a fed-batch fermentation, which were 13 and 6.8 times higher than the DM strain in batch culture, respectively. Conclusively, these metabolically-engineered approaches might pave the way for the sustainable production of other sesquiterpenes in engineered S. cerevisiae. (−)-α-Bisabolol Park, Haeseong verfasserin aut Kim, Sun-Ki verfasserin aut Kim, Soo-Jung verfasserin aut Park, Yong-Cheol verfasserin (orcid)0000-0002-0331-078X aut Enthalten in Journal of biotechnology Amsterdam [u.a.] : Elsevier Science, 1984 340, Seite 13-21 Online-Ressource (DE-627)320570851 (DE-600)2016476-2 (DE-576)090956125 1873-4863 nnns volume:340 pages:13-21 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie AR 340 13-21
spelling	10.1016/j.jbiotec.2021.08.008 doi (DE-627)ELV006721796 (ELSEVIER)S0168-1656(21)00219-4 DE-627 ger DE-627 rda eng 540 DE-600 58.30 bkl Kim, Tae Yeob verfasserin aut Production of (−)-α-bisabolol in metabolically engineered 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The codon-optimized MrBBS gene coding for (−)-α-bisabolol synthase from Matricaria recutita was expressed in S. cerevisiae for (−)-α-bisabolol production. The resulting strain (DM) produced 9.5 mg/L of (−)-α-bisabolol in 24 h of batch culture. Additionally, the mevalonate pathway was intensified by introducing a truncated HMG1 gene coding for HMG-CoA reductase and ERG10 encoding acetyl-CoA thiolase. The resulting strain (DtEM) produced a 2.9-fold increased concentration of (−)-α-bisabolol than the DM strain. To increase the acetyl-CoA pool, the ACS1 gene coding for acetyl-CoA synthetase was also overexpressed in the DtEM strain. Finally, the DtEMA strain produced 124 mg/L of (−)-α-bisabolol with 2.7 mg/L-h of productivity in a fed-batch fermentation, which were 13 and 6.8 times higher than the DM strain in batch culture, respectively. Conclusively, these metabolically-engineered approaches might pave the way for the sustainable production of other sesquiterpenes in engineered S. cerevisiae. (−)-α-Bisabolol Park, Haeseong verfasserin aut Kim, Sun-Ki verfasserin aut Kim, Soo-Jung verfasserin aut Park, Yong-Cheol verfasserin (orcid)0000-0002-0331-078X aut Enthalten in Journal of biotechnology Amsterdam [u.a.] : Elsevier Science, 1984 340, Seite 13-21 Online-Ressource (DE-627)320570851 (DE-600)2016476-2 (DE-576)090956125 1873-4863 nnns volume:340 pages:13-21 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie AR 340 13-21
allfields_unstemmed	10.1016/j.jbiotec.2021.08.008 doi (DE-627)ELV006721796 (ELSEVIER)S0168-1656(21)00219-4 DE-627 ger DE-627 rda eng 540 DE-600 58.30 bkl Kim, Tae Yeob verfasserin aut Production of (−)-α-bisabolol in metabolically engineered 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The codon-optimized MrBBS gene coding for (−)-α-bisabolol synthase from Matricaria recutita was expressed in S. cerevisiae for (−)-α-bisabolol production. The resulting strain (DM) produced 9.5 mg/L of (−)-α-bisabolol in 24 h of batch culture. Additionally, the mevalonate pathway was intensified by introducing a truncated HMG1 gene coding for HMG-CoA reductase and ERG10 encoding acetyl-CoA thiolase. The resulting strain (DtEM) produced a 2.9-fold increased concentration of (−)-α-bisabolol than the DM strain. To increase the acetyl-CoA pool, the ACS1 gene coding for acetyl-CoA synthetase was also overexpressed in the DtEM strain. Finally, the DtEMA strain produced 124 mg/L of (−)-α-bisabolol with 2.7 mg/L-h of productivity in a fed-batch fermentation, which were 13 and 6.8 times higher than the DM strain in batch culture, respectively. Conclusively, these metabolically-engineered approaches might pave the way for the sustainable production of other sesquiterpenes in engineered S. cerevisiae. (−)-α-Bisabolol Park, Haeseong verfasserin aut Kim, Sun-Ki verfasserin aut Kim, Soo-Jung verfasserin aut Park, Yong-Cheol verfasserin (orcid)0000-0002-0331-078X aut Enthalten in Journal of biotechnology Amsterdam [u.a.] : Elsevier Science, 1984 340, Seite 13-21 Online-Ressource (DE-627)320570851 (DE-600)2016476-2 (DE-576)090956125 1873-4863 nnns volume:340 pages:13-21 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie AR 340 13-21
allfieldsGer	10.1016/j.jbiotec.2021.08.008 doi (DE-627)ELV006721796 (ELSEVIER)S0168-1656(21)00219-4 DE-627 ger DE-627 rda eng 540 DE-600 58.30 bkl Kim, Tae Yeob verfasserin aut Production of (−)-α-bisabolol in metabolically engineered 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The codon-optimized MrBBS gene coding for (−)-α-bisabolol synthase from Matricaria recutita was expressed in S. cerevisiae for (−)-α-bisabolol production. The resulting strain (DM) produced 9.5 mg/L of (−)-α-bisabolol in 24 h of batch culture. Additionally, the mevalonate pathway was intensified by introducing a truncated HMG1 gene coding for HMG-CoA reductase and ERG10 encoding acetyl-CoA thiolase. The resulting strain (DtEM) produced a 2.9-fold increased concentration of (−)-α-bisabolol than the DM strain. To increase the acetyl-CoA pool, the ACS1 gene coding for acetyl-CoA synthetase was also overexpressed in the DtEM strain. Finally, the DtEMA strain produced 124 mg/L of (−)-α-bisabolol with 2.7 mg/L-h of productivity in a fed-batch fermentation, which were 13 and 6.8 times higher than the DM strain in batch culture, respectively. Conclusively, these metabolically-engineered approaches might pave the way for the sustainable production of other sesquiterpenes in engineered S. cerevisiae. (−)-α-Bisabolol Park, Haeseong verfasserin aut Kim, Sun-Ki verfasserin aut Kim, Soo-Jung verfasserin aut Park, Yong-Cheol verfasserin (orcid)0000-0002-0331-078X aut Enthalten in Journal of biotechnology Amsterdam [u.a.] : Elsevier Science, 1984 340, Seite 13-21 Online-Ressource (DE-627)320570851 (DE-600)2016476-2 (DE-576)090956125 1873-4863 nnns volume:340 pages:13-21 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie AR 340 13-21
allfieldsSound	10.1016/j.jbiotec.2021.08.008 doi (DE-627)ELV006721796 (ELSEVIER)S0168-1656(21)00219-4 DE-627 ger DE-627 rda eng 540 DE-600 58.30 bkl Kim, Tae Yeob verfasserin aut Production of (−)-α-bisabolol in metabolically engineered 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier (−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The codon-optimized MrBBS gene coding for (−)-α-bisabolol synthase from Matricaria recutita was expressed in S. cerevisiae for (−)-α-bisabolol production. The resulting strain (DM) produced 9.5 mg/L of (−)-α-bisabolol in 24 h of batch culture. Additionally, the mevalonate pathway was intensified by introducing a truncated HMG1 gene coding for HMG-CoA reductase and ERG10 encoding acetyl-CoA thiolase. The resulting strain (DtEM) produced a 2.9-fold increased concentration of (−)-α-bisabolol than the DM strain. To increase the acetyl-CoA pool, the ACS1 gene coding for acetyl-CoA synthetase was also overexpressed in the DtEM strain. Finally, the DtEMA strain produced 124 mg/L of (−)-α-bisabolol with 2.7 mg/L-h of productivity in a fed-batch fermentation, which were 13 and 6.8 times higher than the DM strain in batch culture, respectively. Conclusively, these metabolically-engineered approaches might pave the way for the sustainable production of other sesquiterpenes in engineered S. cerevisiae. (−)-α-Bisabolol Park, Haeseong verfasserin aut Kim, Sun-Ki verfasserin aut Kim, Soo-Jung verfasserin aut Park, Yong-Cheol verfasserin (orcid)0000-0002-0331-078X aut Enthalten in Journal of biotechnology Amsterdam [u.a.] : Elsevier Science, 1984 340, Seite 13-21 Online-Ressource (DE-627)320570851 (DE-600)2016476-2 (DE-576)090956125 1873-4863 nnns volume:340 pages:13-21 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.30 Biotechnologie AR 340 13-21
language	English
source	Enthalten in Journal of biotechnology 340, Seite 13-21 volume:340 pages:13-21
sourceStr	Enthalten in Journal of biotechnology 340, Seite 13-21 volume:340 pages:13-21
format_phy_str_mv	Article
bklname	Biotechnologie
institution	findex.gbv.de
topic_facet	(−)-α-Bisabolol
dewey-raw	540
isfreeaccess_bool	false
container_title	Journal of biotechnology
authorswithroles_txt_mv	Kim, Tae Yeob @@aut@@ Park, Haeseong @@aut@@ Kim, Sun-Ki @@aut@@ Kim, Soo-Jung @@aut@@ Park, Yong-Cheol @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	320570851
dewey-sort	3540
id	ELV006721796
language_de	englisch
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author	Kim, Tae Yeob
spellingShingle	Kim, Tae Yeob ddc 540 bkl 58.30 misc (−)-α-Bisabolol Production of (−)-α-bisabolol in metabolically engineered
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topic_title	540 DE-600 58.30 bkl Production of (−)-α-bisabolol in metabolically engineered (−)-α-Bisabolol
topic	ddc 540 bkl 58.30 misc (−)-α-Bisabolol
topic_unstemmed	ddc 540 bkl 58.30 misc (−)-α-Bisabolol
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title	Production of (−)-α-bisabolol in metabolically engineered
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title_full	Production of (−)-α-bisabolol in metabolically engineered
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author_browse	Kim, Tae Yeob Park, Haeseong Kim, Sun-Ki Kim, Soo-Jung Park, Yong-Cheol
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author-letter	Kim, Tae Yeob
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title_sort	production of (−)-α-bisabolol in metabolically engineered
title_auth	Production of (−)-α-bisabolol in metabolically engineered
abstract	(−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The codon-optimized MrBBS gene coding for (−)-α-bisabolol synthase from Matricaria recutita was expressed in S. cerevisiae for (−)-α-bisabolol production. The resulting strain (DM) produced 9.5 mg/L of (−)-α-bisabolol in 24 h of batch culture. Additionally, the mevalonate pathway was intensified by introducing a truncated HMG1 gene coding for HMG-CoA reductase and ERG10 encoding acetyl-CoA thiolase. The resulting strain (DtEM) produced a 2.9-fold increased concentration of (−)-α-bisabolol than the DM strain. To increase the acetyl-CoA pool, the ACS1 gene coding for acetyl-CoA synthetase was also overexpressed in the DtEM strain. Finally, the DtEMA strain produced 124 mg/L of (−)-α-bisabolol with 2.7 mg/L-h of productivity in a fed-batch fermentation, which were 13 and 6.8 times higher than the DM strain in batch culture, respectively. Conclusively, these metabolically-engineered approaches might pave the way for the sustainable production of other sesquiterpenes in engineered S. cerevisiae.
abstractGer	(−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The codon-optimized MrBBS gene coding for (−)-α-bisabolol synthase from Matricaria recutita was expressed in S. cerevisiae for (−)-α-bisabolol production. The resulting strain (DM) produced 9.5 mg/L of (−)-α-bisabolol in 24 h of batch culture. Additionally, the mevalonate pathway was intensified by introducing a truncated HMG1 gene coding for HMG-CoA reductase and ERG10 encoding acetyl-CoA thiolase. The resulting strain (DtEM) produced a 2.9-fold increased concentration of (−)-α-bisabolol than the DM strain. To increase the acetyl-CoA pool, the ACS1 gene coding for acetyl-CoA synthetase was also overexpressed in the DtEM strain. Finally, the DtEMA strain produced 124 mg/L of (−)-α-bisabolol with 2.7 mg/L-h of productivity in a fed-batch fermentation, which were 13 and 6.8 times higher than the DM strain in batch culture, respectively. Conclusively, these metabolically-engineered approaches might pave the way for the sustainable production of other sesquiterpenes in engineered S. cerevisiae.
abstract_unstemmed	(−)-α-Bisabolol is a natural monocyclic sesquiterpene alcohol present in German chamomile and has been used as an ingredient of functional foods, cosmetics and pharmaceuticals. In this study, metabolic engineering strategies were attempted to produce (−)-α-bisabolol in Saccharomyces cerevisiae. The codon-optimized MrBBS gene coding for (−)-α-bisabolol synthase from Matricaria recutita was expressed in S. cerevisiae for (−)-α-bisabolol production. The resulting strain (DM) produced 9.5 mg/L of (−)-α-bisabolol in 24 h of batch culture. Additionally, the mevalonate pathway was intensified by introducing a truncated HMG1 gene coding for HMG-CoA reductase and ERG10 encoding acetyl-CoA thiolase. The resulting strain (DtEM) produced a 2.9-fold increased concentration of (−)-α-bisabolol than the DM strain. To increase the acetyl-CoA pool, the ACS1 gene coding for acetyl-CoA synthetase was also overexpressed in the DtEM strain. Finally, the DtEMA strain produced 124 mg/L of (−)-α-bisabolol with 2.7 mg/L-h of productivity in a fed-batch fermentation, which were 13 and 6.8 times higher than the DM strain in batch culture, respectively. Conclusively, these metabolically-engineered approaches might pave the way for the sustainable production of other sesquiterpenes in engineered S. cerevisiae.
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title_short	Production of (−)-α-bisabolol in metabolically engineered
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author2	Park, Haeseong Kim, Sun-Ki Kim, Soo-Jung Park, Yong-Cheol
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up_date	2024-07-06T22:20:35.078Z
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Production of (−)-α-bisabolol in metabolically engineered

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