New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin

A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit f...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Yoo, Soyeon [verfasserIn] Han, Min Su [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Mono- and dipeptidyl-carboxypeptidase Fluorescent substrates Angiotensin-converting enzyme Carboxypeptidase B Signal amplification Albumin

Übergeordnetes Werk:	Enthalten in: Dyes and pigments - Amsterdam [u.a.] : Elsevier Science, 1980, 196
Übergeordnetes Werk:	volume:196

DOI / URN:	10.1016/j.dyepig.2021.109804

Katalog-ID:	ELV006726194

Internformat


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024	7		\|a 10.1016/j.dyepig.2021.109804 \|2 doi
035			\|a (DE-627)ELV006726194
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100	1		\|a Yoo, Soyeon \|e verfasserin \|4 aut
245	1	0	\|a New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin
264		1	\|c 2021
336			\|a nicht spezifiziert \|b zzz \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
338			\|a Online-Ressource \|b cr \|2 rdacarrier
520			\|a A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit for CP, to the C-terminus of DS. In the presence of the target CP, the low-fluorescence substrate is hydrolyzed to a strongly fluorescent DS by binding with albumin. As a proof concept, Dansyl-Sar-Lys-Pro (DS-KP) and Dansyl-Sar-Arg (DS-R) were developed as fluorescent substrates for the angiotensin-converting enzyme and carboxypeptidase B, respectively. The CP assay system was verified to be capable of measuring the activities of both dipeptidyl-CP and mono-CP and the inhibition efficiency of various CP inhibitors, confirming its applicability as a high-throughput screening method for numerous inhibitor candidates.
650		4	\|a Mono- and dipeptidyl-carboxypeptidase
650		4	\|a Fluorescent substrates
650		4	\|a Angiotensin-converting enzyme
650		4	\|a Carboxypeptidase B
650		4	\|a Signal amplification
650		4	\|a Albumin
700	1		\|a Han, Min Su \|e verfasserin \|0 (orcid)0000-0001-9588-6980 \|4 aut
773	0	8	\|i Enthalten in \|t Dyes and pigments \|d Amsterdam [u.a.] : Elsevier Science, 1980 \|g 196 \|h Online-Ressource \|w (DE-627)306658755 \|w (DE-600)1500382-6 \|w (DE-576)116550910 \|x 0143-7208 \|7 nnns
773	1	8	\|g volume:196
912			\|a GBV_USEFLAG_U
912			\|a SYSFLAG_U
912			\|a GBV_ELV
912			\|a SSG-OLC-PHA
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_224
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2006
912			\|a GBV_ILN_2008
912			\|a GBV_ILN_2010
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2470
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4046
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 58.26 \|j Technologie der Farben und Lacke
951			\|a AR
952			\|d 196

Indexfelder

author_variant	s y sy m s h ms msh
matchkey_str	article:01437208:2021----::esrtgtdsgfursetusrtsfabxppiaeuigcmiainf
hierarchy_sort_str	2021
bklnumber	58.26
publishDate	2021
allfields	10.1016/j.dyepig.2021.109804 doi (DE-627)ELV006726194 (ELSEVIER)S0143-7208(21)00670-7 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Yoo, Soyeon verfasserin aut New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit for CP, to the C-terminus of DS. In the presence of the target CP, the low-fluorescence substrate is hydrolyzed to a strongly fluorescent DS by binding with albumin. As a proof concept, Dansyl-Sar-Lys-Pro (DS-KP) and Dansyl-Sar-Arg (DS-R) were developed as fluorescent substrates for the angiotensin-converting enzyme and carboxypeptidase B, respectively. The CP assay system was verified to be capable of measuring the activities of both dipeptidyl-CP and mono-CP and the inhibition efficiency of various CP inhibitors, confirming its applicability as a high-throughput screening method for numerous inhibitor candidates. Mono- and dipeptidyl-carboxypeptidase Fluorescent substrates Angiotensin-converting enzyme Carboxypeptidase B Signal amplification Albumin Han, Min Su verfasserin (orcid)0000-0001-9588-6980 aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 196 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:196 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 196
spelling	10.1016/j.dyepig.2021.109804 doi (DE-627)ELV006726194 (ELSEVIER)S0143-7208(21)00670-7 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Yoo, Soyeon verfasserin aut New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit for CP, to the C-terminus of DS. In the presence of the target CP, the low-fluorescence substrate is hydrolyzed to a strongly fluorescent DS by binding with albumin. As a proof concept, Dansyl-Sar-Lys-Pro (DS-KP) and Dansyl-Sar-Arg (DS-R) were developed as fluorescent substrates for the angiotensin-converting enzyme and carboxypeptidase B, respectively. The CP assay system was verified to be capable of measuring the activities of both dipeptidyl-CP and mono-CP and the inhibition efficiency of various CP inhibitors, confirming its applicability as a high-throughput screening method for numerous inhibitor candidates. Mono- and dipeptidyl-carboxypeptidase Fluorescent substrates Angiotensin-converting enzyme Carboxypeptidase B Signal amplification Albumin Han, Min Su verfasserin (orcid)0000-0001-9588-6980 aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 196 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:196 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 196
allfields_unstemmed	10.1016/j.dyepig.2021.109804 doi (DE-627)ELV006726194 (ELSEVIER)S0143-7208(21)00670-7 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Yoo, Soyeon verfasserin aut New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit for CP, to the C-terminus of DS. In the presence of the target CP, the low-fluorescence substrate is hydrolyzed to a strongly fluorescent DS by binding with albumin. As a proof concept, Dansyl-Sar-Lys-Pro (DS-KP) and Dansyl-Sar-Arg (DS-R) were developed as fluorescent substrates for the angiotensin-converting enzyme and carboxypeptidase B, respectively. The CP assay system was verified to be capable of measuring the activities of both dipeptidyl-CP and mono-CP and the inhibition efficiency of various CP inhibitors, confirming its applicability as a high-throughput screening method for numerous inhibitor candidates. Mono- and dipeptidyl-carboxypeptidase Fluorescent substrates Angiotensin-converting enzyme Carboxypeptidase B Signal amplification Albumin Han, Min Su verfasserin (orcid)0000-0001-9588-6980 aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 196 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:196 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 196
allfieldsGer	10.1016/j.dyepig.2021.109804 doi (DE-627)ELV006726194 (ELSEVIER)S0143-7208(21)00670-7 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Yoo, Soyeon verfasserin aut New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit for CP, to the C-terminus of DS. In the presence of the target CP, the low-fluorescence substrate is hydrolyzed to a strongly fluorescent DS by binding with albumin. As a proof concept, Dansyl-Sar-Lys-Pro (DS-KP) and Dansyl-Sar-Arg (DS-R) were developed as fluorescent substrates for the angiotensin-converting enzyme and carboxypeptidase B, respectively. The CP assay system was verified to be capable of measuring the activities of both dipeptidyl-CP and mono-CP and the inhibition efficiency of various CP inhibitors, confirming its applicability as a high-throughput screening method for numerous inhibitor candidates. Mono- and dipeptidyl-carboxypeptidase Fluorescent substrates Angiotensin-converting enzyme Carboxypeptidase B Signal amplification Albumin Han, Min Su verfasserin (orcid)0000-0001-9588-6980 aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 196 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:196 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 196
allfieldsSound	10.1016/j.dyepig.2021.109804 doi (DE-627)ELV006726194 (ELSEVIER)S0143-7208(21)00670-7 DE-627 ger DE-627 rda eng 660 DE-600 58.26 bkl Yoo, Soyeon verfasserin aut New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit for CP, to the C-terminus of DS. In the presence of the target CP, the low-fluorescence substrate is hydrolyzed to a strongly fluorescent DS by binding with albumin. As a proof concept, Dansyl-Sar-Lys-Pro (DS-KP) and Dansyl-Sar-Arg (DS-R) were developed as fluorescent substrates for the angiotensin-converting enzyme and carboxypeptidase B, respectively. The CP assay system was verified to be capable of measuring the activities of both dipeptidyl-CP and mono-CP and the inhibition efficiency of various CP inhibitors, confirming its applicability as a high-throughput screening method for numerous inhibitor candidates. Mono- and dipeptidyl-carboxypeptidase Fluorescent substrates Angiotensin-converting enzyme Carboxypeptidase B Signal amplification Albumin Han, Min Su verfasserin (orcid)0000-0001-9588-6980 aut Enthalten in Dyes and pigments Amsterdam [u.a.] : Elsevier Science, 1980 196 Online-Ressource (DE-627)306658755 (DE-600)1500382-6 (DE-576)116550910 0143-7208 nnns volume:196 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2006 GBV_ILN_2008 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4046 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 58.26 Technologie der Farben und Lacke AR 196
language	English
source	Enthalten in Dyes and pigments 196 volume:196
sourceStr	Enthalten in Dyes and pigments 196 volume:196
format_phy_str_mv	Article
bklname	Technologie der Farben und Lacke
institution	findex.gbv.de
topic_facet	Mono- and dipeptidyl-carboxypeptidase Fluorescent substrates Angiotensin-converting enzyme Carboxypeptidase B Signal amplification Albumin
dewey-raw	660
isfreeaccess_bool	false
container_title	Dyes and pigments
authorswithroles_txt_mv	Yoo, Soyeon @@aut@@ Han, Min Su @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	306658755
dewey-sort	3660
id	ELV006726194
language_de	englisch
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author	Yoo, Soyeon
spellingShingle	Yoo, Soyeon ddc 660 bkl 58.26 misc Mono- and dipeptidyl-carboxypeptidase misc Fluorescent substrates misc Angiotensin-converting enzyme misc Carboxypeptidase B misc Signal amplification misc Albumin New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin
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topic_title	660 DE-600 58.26 bkl New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin Mono- and dipeptidyl-carboxypeptidase Fluorescent substrates Angiotensin-converting enzyme Carboxypeptidase B Signal amplification Albumin
topic	ddc 660 bkl 58.26 misc Mono- and dipeptidyl-carboxypeptidase misc Fluorescent substrates misc Angiotensin-converting enzyme misc Carboxypeptidase B misc Signal amplification misc Albumin
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title	New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin
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title_full	New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin
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title_sort	new strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin
title_auth	New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin
abstract	A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit for CP, to the C-terminus of DS. In the presence of the target CP, the low-fluorescence substrate is hydrolyzed to a strongly fluorescent DS by binding with albumin. As a proof concept, Dansyl-Sar-Lys-Pro (DS-KP) and Dansyl-Sar-Arg (DS-R) were developed as fluorescent substrates for the angiotensin-converting enzyme and carboxypeptidase B, respectively. The CP assay system was verified to be capable of measuring the activities of both dipeptidyl-CP and mono-CP and the inhibition efficiency of various CP inhibitors, confirming its applicability as a high-throughput screening method for numerous inhibitor candidates.
abstractGer	A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit for CP, to the C-terminus of DS. In the presence of the target CP, the low-fluorescence substrate is hydrolyzed to a strongly fluorescent DS by binding with albumin. As a proof concept, Dansyl-Sar-Lys-Pro (DS-KP) and Dansyl-Sar-Arg (DS-R) were developed as fluorescent substrates for the angiotensin-converting enzyme and carboxypeptidase B, respectively. The CP assay system was verified to be capable of measuring the activities of both dipeptidyl-CP and mono-CP and the inhibition efficiency of various CP inhibitors, confirming its applicability as a high-throughput screening method for numerous inhibitor candidates.
abstract_unstemmed	A new strategy to design a fluorescent substrate for carboxypeptidases (CPs) was devised using dansylated sarcosine (DS) and albumin as a fluorophore and signal amplifier, respectively. The fluorescent substrate was designed by attaching an amino acid or peptide, which acts as the recognition unit for CP, to the C-terminus of DS. In the presence of the target CP, the low-fluorescence substrate is hydrolyzed to a strongly fluorescent DS by binding with albumin. As a proof concept, Dansyl-Sar-Lys-Pro (DS-KP) and Dansyl-Sar-Arg (DS-R) were developed as fluorescent substrates for the angiotensin-converting enzyme and carboxypeptidase B, respectively. The CP assay system was verified to be capable of measuring the activities of both dipeptidyl-CP and mono-CP and the inhibition efficiency of various CP inhibitors, confirming its applicability as a high-throughput screening method for numerous inhibitor candidates.
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title_short	New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin
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doi_str	10.1016/j.dyepig.2021.109804
up_date	2024-07-06T22:21:29.839Z
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New strategy to design fluorescent substrates of carboxypeptidases using a combination of dansylated peptides and albumin

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