Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies

Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to sel...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Segués, Aina [verfasserIn] van Duijnhoven, Sander M.J. [verfasserIn] Parade, Marc [verfasserIn] Driessen, Lilian [verfasserIn] Vukovic, Nataša [verfasserIn] Zaiss, Dietmar [verfasserIn] Sijts, Alice J.A.M. [verfasserIn] Berraondo, Pedro [verfasserIn] van Elsas, Andrea [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	TNFR2 Antibody Epitope Cysteine-rich domain Costimulation Treg

Übergeordnetes Werk:	Enthalten in: Journal of immunological methods - Amsterdam [u.a.] : Elsevier Science, 1971, 499
Übergeordnetes Werk:	volume:499

DOI / URN:	10.1016/j.jim.2021.113173

Katalog-ID:	ELV006948693

Internformat


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245	1	0	\|a Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies
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520			\|a Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer.
650		4	\|a TNFR2
650		4	\|a Antibody
650		4	\|a Epitope
650		4	\|a Cysteine-rich domain
650		4	\|a Costimulation
650		4	\|a Treg
700	1		\|a van Duijnhoven, Sander M.J. \|e verfasserin \|4 aut
700	1		\|a Parade, Marc \|e verfasserin \|4 aut
700	1		\|a Driessen, Lilian \|e verfasserin \|4 aut
700	1		\|a Vukovic, Nataša \|e verfasserin \|4 aut
700	1		\|a Zaiss, Dietmar \|e verfasserin \|4 aut
700	1		\|a Sijts, Alice J.A.M. \|e verfasserin \|4 aut
700	1		\|a Berraondo, Pedro \|e verfasserin \|4 aut
700	1		\|a van Elsas, Andrea \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Journal of immunological methods \|d Amsterdam [u.a.] : Elsevier Science, 1971 \|g 499 \|h Online-Ressource \|w (DE-627)306659751 \|w (DE-600)1500495-8 \|w (DE-576)081986386 \|x 1872-7905 \|7 nnns
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912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_224
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 44.45 \|j Immunologie
951			\|a AR
952			\|d 499

Indexfelder

author_variant	a s as d s m v dsm dsmv m p mp l d ld n v nv d z dz a j s aj ajs p b pb e a v ea eav
matchkey_str	article:18727905:2021----::eeainncaatrztoonvlotmltratm
hierarchy_sort_str	2021
bklnumber	44.45
publishDate	2021
allfields	10.1016/j.jim.2021.113173 doi (DE-627)ELV006948693 (ELSEVIER)S0022-1759(21)00218-0 DE-627 ger DE-627 rda eng 610 DE-600 44.45 bkl Segués, Aina verfasserin aut Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer. TNFR2 Antibody Epitope Cysteine-rich domain Costimulation Treg van Duijnhoven, Sander M.J. verfasserin aut Parade, Marc verfasserin aut Driessen, Lilian verfasserin aut Vukovic, Nataša verfasserin aut Zaiss, Dietmar verfasserin aut Sijts, Alice J.A.M. verfasserin aut Berraondo, Pedro verfasserin aut van Elsas, Andrea verfasserin aut Enthalten in Journal of immunological methods Amsterdam [u.a.] : Elsevier Science, 1971 499 Online-Ressource (DE-627)306659751 (DE-600)1500495-8 (DE-576)081986386 1872-7905 nnns volume:499 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.45 Immunologie AR 499
spelling	10.1016/j.jim.2021.113173 doi (DE-627)ELV006948693 (ELSEVIER)S0022-1759(21)00218-0 DE-627 ger DE-627 rda eng 610 DE-600 44.45 bkl Segués, Aina verfasserin aut Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer. TNFR2 Antibody Epitope Cysteine-rich domain Costimulation Treg van Duijnhoven, Sander M.J. verfasserin aut Parade, Marc verfasserin aut Driessen, Lilian verfasserin aut Vukovic, Nataša verfasserin aut Zaiss, Dietmar verfasserin aut Sijts, Alice J.A.M. verfasserin aut Berraondo, Pedro verfasserin aut van Elsas, Andrea verfasserin aut Enthalten in Journal of immunological methods Amsterdam [u.a.] : Elsevier Science, 1971 499 Online-Ressource (DE-627)306659751 (DE-600)1500495-8 (DE-576)081986386 1872-7905 nnns volume:499 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.45 Immunologie AR 499
allfields_unstemmed	10.1016/j.jim.2021.113173 doi (DE-627)ELV006948693 (ELSEVIER)S0022-1759(21)00218-0 DE-627 ger DE-627 rda eng 610 DE-600 44.45 bkl Segués, Aina verfasserin aut Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer. TNFR2 Antibody Epitope Cysteine-rich domain Costimulation Treg van Duijnhoven, Sander M.J. verfasserin aut Parade, Marc verfasserin aut Driessen, Lilian verfasserin aut Vukovic, Nataša verfasserin aut Zaiss, Dietmar verfasserin aut Sijts, Alice J.A.M. verfasserin aut Berraondo, Pedro verfasserin aut van Elsas, Andrea verfasserin aut Enthalten in Journal of immunological methods Amsterdam [u.a.] : Elsevier Science, 1971 499 Online-Ressource (DE-627)306659751 (DE-600)1500495-8 (DE-576)081986386 1872-7905 nnns volume:499 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.45 Immunologie AR 499
allfieldsGer	10.1016/j.jim.2021.113173 doi (DE-627)ELV006948693 (ELSEVIER)S0022-1759(21)00218-0 DE-627 ger DE-627 rda eng 610 DE-600 44.45 bkl Segués, Aina verfasserin aut Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer. TNFR2 Antibody Epitope Cysteine-rich domain Costimulation Treg van Duijnhoven, Sander M.J. verfasserin aut Parade, Marc verfasserin aut Driessen, Lilian verfasserin aut Vukovic, Nataša verfasserin aut Zaiss, Dietmar verfasserin aut Sijts, Alice J.A.M. verfasserin aut Berraondo, Pedro verfasserin aut van Elsas, Andrea verfasserin aut Enthalten in Journal of immunological methods Amsterdam [u.a.] : Elsevier Science, 1971 499 Online-Ressource (DE-627)306659751 (DE-600)1500495-8 (DE-576)081986386 1872-7905 nnns volume:499 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.45 Immunologie AR 499
allfieldsSound	10.1016/j.jim.2021.113173 doi (DE-627)ELV006948693 (ELSEVIER)S0022-1759(21)00218-0 DE-627 ger DE-627 rda eng 610 DE-600 44.45 bkl Segués, Aina verfasserin aut Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer. TNFR2 Antibody Epitope Cysteine-rich domain Costimulation Treg van Duijnhoven, Sander M.J. verfasserin aut Parade, Marc verfasserin aut Driessen, Lilian verfasserin aut Vukovic, Nataša verfasserin aut Zaiss, Dietmar verfasserin aut Sijts, Alice J.A.M. verfasserin aut Berraondo, Pedro verfasserin aut van Elsas, Andrea verfasserin aut Enthalten in Journal of immunological methods Amsterdam [u.a.] : Elsevier Science, 1971 499 Online-Ressource (DE-627)306659751 (DE-600)1500495-8 (DE-576)081986386 1872-7905 nnns volume:499 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.45 Immunologie AR 499
language	English
source	Enthalten in Journal of immunological methods 499 volume:499
sourceStr	Enthalten in Journal of immunological methods 499 volume:499
format_phy_str_mv	Article
bklname	Immunologie
institution	findex.gbv.de
topic_facet	TNFR2 Antibody Epitope Cysteine-rich domain Costimulation Treg
dewey-raw	610
isfreeaccess_bool	false
container_title	Journal of immunological methods
authorswithroles_txt_mv	Segués, Aina @@aut@@ van Duijnhoven, Sander M.J. @@aut@@ Parade, Marc @@aut@@ Driessen, Lilian @@aut@@ Vukovic, Nataša @@aut@@ Zaiss, Dietmar @@aut@@ Sijts, Alice J.A.M. @@aut@@ Berraondo, Pedro @@aut@@ van Elsas, Andrea @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	306659751
dewey-sort	3610
id	ELV006948693
language_de	englisch
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author	Segués, Aina
spellingShingle	Segués, Aina ddc 610 bkl 44.45 misc TNFR2 misc Antibody misc Epitope misc Cysteine-rich domain misc Costimulation misc Treg Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies
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topic_title	610 DE-600 44.45 bkl Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies TNFR2 Antibody Epitope Cysteine-rich domain Costimulation Treg
topic	ddc 610 bkl 44.45 misc TNFR2 misc Antibody misc Epitope misc Cysteine-rich domain misc Costimulation misc Treg
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title	Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies
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title_full	Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies
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author_browse	Segués, Aina van Duijnhoven, Sander M.J. Parade, Marc Driessen, Lilian Vukovic, Nataša Zaiss, Dietmar Sijts, Alice J.A.M. Berraondo, Pedro van Elsas, Andrea
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title_sort	generation and characterization of novel co-stimulatory anti-mouse tnfr2 antibodies
title_auth	Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies
abstract	Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer.
abstractGer	Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer.
abstract_unstemmed	Tumor necrosis factor receptor 2 (TNFR2) has gained much research interest in recent years because of its potential pivotal role in autoimmune disease and cancer. However, its function in regulating different immune cells is not well understood. There is a need for well-characterized reagents to selectively modulate TNFR2 function, thereby enabling definition of TNFR2-dependent biology in human and mouse surrogate models. Here, we describe the generation, production, purification, and characterization of a panel of novel antibodies targeting mouse TNFR2. The antibodies display functional differences in binding affinity and potency to block TNFα. Furthermore, epitope binding showed that the anti-mTNFR2 antibodies target different domains on the TNFR2 protein, associated with varying capacity to enhance CD8+ T-cell activation and costimulation. Moreover, the anti-TNFR2 antibodies demonstrate binding to isolated splenic mouse Tregs ex vivo and activated CD8+ cells, reinforcing their potential use to establish TNFR2-dependent immune modulation in translational models of autoimmunity and cancer.
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title_short	Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies
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author2	van Duijnhoven, Sander M.J. Parade, Marc Driessen, Lilian Vukovic, Nataša Zaiss, Dietmar Sijts, Alice J.A.M. Berraondo, Pedro van Elsas, Andrea
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up_date	2024-07-06T23:06:05.545Z
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Generation and characterization of novel co-stimulatory anti-mouse TNFR2 antibodies

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