Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites

Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatib...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Zhang, Yinghao [verfasserIn] Xu, Qiao [verfasserIn] Wang, Feiyu [verfasserIn] Gao, Tao [verfasserIn] Wei, Tianxiang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Enzyme powered self-assembly Hydrogel biosensor Colorimetric detection of metabolites One-tube assay kit

Übergeordnetes Werk:	Enthalten in: Sensors and actuators / B - Amsterdam [u.a.] : Elsevier Science, 1990, 375
Übergeordnetes Werk:	volume:375

DOI / URN:	10.1016/j.snb.2022.132942

Katalog-ID:	ELV008789614

Internformat


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245	1	0	\|a Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites
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337			\|a Computermedien \|b c \|2 rdamedia
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520			\|a Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatible for encapsulation of detection enzymes while retain their activities, so as to facilitate catalytic detection of specific metabolites with colorimetric signal readout. For convenience of the operation, the hydrogel biosensor has been further fitted in a test tube to construct a new kind of one-tube assay kit. For the proof-of-concept, assay kits for hydrogen peroxide (H2O2) and glucose have been testified. The linear detection ranges for H2O2 and glucose are 0.05–1.50 mM and 2–50 mM, the limit of detection (LOD) are 8.9 µM and 1.6 mM, and the entire assay time require only 1 min and 6 min, respectively. They exhibit high sensitivity and specificity both in test solution and real serum samples. Therefore, this one-tube colorimetric assay kit is promising for in home medical diagnosis and clinical routine rapid testing. Additionally, the hydrogel biosensor system may have universality for embedding other detecting enzymes, and enable many biomedical tests based on specific enzymes.
650		4	\|a Enzyme powered self-assembly
650		4	\|a Hydrogel biosensor
650		4	\|a Colorimetric detection of metabolites
650		4	\|a One-tube assay kit
700	1		\|a Xu, Qiao \|e verfasserin \|4 aut
700	1		\|a Wang, Feiyu \|e verfasserin \|4 aut
700	1		\|a Gao, Tao \|e verfasserin \|4 aut
700	1		\|a Wei, Tianxiang \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Sensors and actuators <Lausanne> / B \|d Amsterdam [u.a.] : Elsevier Science, 1990 \|g 375 \|h Online-Ressource \|w (DE-627)306710358 \|w (DE-600)1500731-5 \|w (DE-576)082435855 \|x 0925-4005 \|7 nnns
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912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 50.22 \|j Sensorik
936	b	k	\|a 35.07 \|j Chemisches Labor \|j chemische Methoden
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author_variant	y z yz q x qx f w fw t g tg t w tw
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bklnumber	50.22 35.07
publishDate	2022
allfields	10.1016/j.snb.2022.132942 doi (DE-627)ELV008789614 (ELSEVIER)S0925-4005(22)01585-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Zhang, Yinghao verfasserin aut Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatible for encapsulation of detection enzymes while retain their activities, so as to facilitate catalytic detection of specific metabolites with colorimetric signal readout. For convenience of the operation, the hydrogel biosensor has been further fitted in a test tube to construct a new kind of one-tube assay kit. For the proof-of-concept, assay kits for hydrogen peroxide (H2O2) and glucose have been testified. The linear detection ranges for H2O2 and glucose are 0.05–1.50 mM and 2–50 mM, the limit of detection (LOD) are 8.9 µM and 1.6 mM, and the entire assay time require only 1 min and 6 min, respectively. They exhibit high sensitivity and specificity both in test solution and real serum samples. Therefore, this one-tube colorimetric assay kit is promising for in home medical diagnosis and clinical routine rapid testing. Additionally, the hydrogel biosensor system may have universality for embedding other detecting enzymes, and enable many biomedical tests based on specific enzymes. Enzyme powered self-assembly Hydrogel biosensor Colorimetric detection of metabolites One-tube assay kit Xu, Qiao verfasserin aut Wang, Feiyu verfasserin aut Gao, Tao verfasserin aut Wei, Tianxiang verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 375 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:375 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 375
spelling	10.1016/j.snb.2022.132942 doi (DE-627)ELV008789614 (ELSEVIER)S0925-4005(22)01585-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Zhang, Yinghao verfasserin aut Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatible for encapsulation of detection enzymes while retain their activities, so as to facilitate catalytic detection of specific metabolites with colorimetric signal readout. For convenience of the operation, the hydrogel biosensor has been further fitted in a test tube to construct a new kind of one-tube assay kit. For the proof-of-concept, assay kits for hydrogen peroxide (H2O2) and glucose have been testified. The linear detection ranges for H2O2 and glucose are 0.05–1.50 mM and 2–50 mM, the limit of detection (LOD) are 8.9 µM and 1.6 mM, and the entire assay time require only 1 min and 6 min, respectively. They exhibit high sensitivity and specificity both in test solution and real serum samples. Therefore, this one-tube colorimetric assay kit is promising for in home medical diagnosis and clinical routine rapid testing. Additionally, the hydrogel biosensor system may have universality for embedding other detecting enzymes, and enable many biomedical tests based on specific enzymes. Enzyme powered self-assembly Hydrogel biosensor Colorimetric detection of metabolites One-tube assay kit Xu, Qiao verfasserin aut Wang, Feiyu verfasserin aut Gao, Tao verfasserin aut Wei, Tianxiang verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 375 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:375 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 375
allfields_unstemmed	10.1016/j.snb.2022.132942 doi (DE-627)ELV008789614 (ELSEVIER)S0925-4005(22)01585-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Zhang, Yinghao verfasserin aut Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatible for encapsulation of detection enzymes while retain their activities, so as to facilitate catalytic detection of specific metabolites with colorimetric signal readout. For convenience of the operation, the hydrogel biosensor has been further fitted in a test tube to construct a new kind of one-tube assay kit. For the proof-of-concept, assay kits for hydrogen peroxide (H2O2) and glucose have been testified. The linear detection ranges for H2O2 and glucose are 0.05–1.50 mM and 2–50 mM, the limit of detection (LOD) are 8.9 µM and 1.6 mM, and the entire assay time require only 1 min and 6 min, respectively. They exhibit high sensitivity and specificity both in test solution and real serum samples. Therefore, this one-tube colorimetric assay kit is promising for in home medical diagnosis and clinical routine rapid testing. Additionally, the hydrogel biosensor system may have universality for embedding other detecting enzymes, and enable many biomedical tests based on specific enzymes. Enzyme powered self-assembly Hydrogel biosensor Colorimetric detection of metabolites One-tube assay kit Xu, Qiao verfasserin aut Wang, Feiyu verfasserin aut Gao, Tao verfasserin aut Wei, Tianxiang verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 375 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:375 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 375
allfieldsGer	10.1016/j.snb.2022.132942 doi (DE-627)ELV008789614 (ELSEVIER)S0925-4005(22)01585-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Zhang, Yinghao verfasserin aut Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatible for encapsulation of detection enzymes while retain their activities, so as to facilitate catalytic detection of specific metabolites with colorimetric signal readout. For convenience of the operation, the hydrogel biosensor has been further fitted in a test tube to construct a new kind of one-tube assay kit. For the proof-of-concept, assay kits for hydrogen peroxide (H2O2) and glucose have been testified. The linear detection ranges for H2O2 and glucose are 0.05–1.50 mM and 2–50 mM, the limit of detection (LOD) are 8.9 µM and 1.6 mM, and the entire assay time require only 1 min and 6 min, respectively. They exhibit high sensitivity and specificity both in test solution and real serum samples. Therefore, this one-tube colorimetric assay kit is promising for in home medical diagnosis and clinical routine rapid testing. Additionally, the hydrogel biosensor system may have universality for embedding other detecting enzymes, and enable many biomedical tests based on specific enzymes. Enzyme powered self-assembly Hydrogel biosensor Colorimetric detection of metabolites One-tube assay kit Xu, Qiao verfasserin aut Wang, Feiyu verfasserin aut Gao, Tao verfasserin aut Wei, Tianxiang verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 375 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:375 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 375
allfieldsSound	10.1016/j.snb.2022.132942 doi (DE-627)ELV008789614 (ELSEVIER)S0925-4005(22)01585-4 DE-627 ger DE-627 rda eng 530 620 DE-600 50.22 bkl 35.07 bkl Zhang, Yinghao verfasserin aut Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatible for encapsulation of detection enzymes while retain their activities, so as to facilitate catalytic detection of specific metabolites with colorimetric signal readout. For convenience of the operation, the hydrogel biosensor has been further fitted in a test tube to construct a new kind of one-tube assay kit. For the proof-of-concept, assay kits for hydrogen peroxide (H2O2) and glucose have been testified. The linear detection ranges for H2O2 and glucose are 0.05–1.50 mM and 2–50 mM, the limit of detection (LOD) are 8.9 µM and 1.6 mM, and the entire assay time require only 1 min and 6 min, respectively. They exhibit high sensitivity and specificity both in test solution and real serum samples. Therefore, this one-tube colorimetric assay kit is promising for in home medical diagnosis and clinical routine rapid testing. Additionally, the hydrogel biosensor system may have universality for embedding other detecting enzymes, and enable many biomedical tests based on specific enzymes. Enzyme powered self-assembly Hydrogel biosensor Colorimetric detection of metabolites One-tube assay kit Xu, Qiao verfasserin aut Wang, Feiyu verfasserin aut Gao, Tao verfasserin aut Wei, Tianxiang verfasserin aut Enthalten in Sensors and actuators <Lausanne> / B Amsterdam [u.a.] : Elsevier Science, 1990 375 Online-Ressource (DE-627)306710358 (DE-600)1500731-5 (DE-576)082435855 0925-4005 nnns volume:375 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 50.22 Sensorik 35.07 Chemisches Labor chemische Methoden AR 375
language	English
source	Enthalten in Sensors and actuators <Lausanne> / B 375 volume:375
sourceStr	Enthalten in Sensors and actuators <Lausanne> / B 375 volume:375
format_phy_str_mv	Article
bklname	Sensorik Chemisches Labor chemische Methoden
institution	findex.gbv.de
topic_facet	Enzyme powered self-assembly Hydrogel biosensor Colorimetric detection of metabolites One-tube assay kit
dewey-raw	530
isfreeaccess_bool	false
container_title	Sensors and actuators <Lausanne> / B
authorswithroles_txt_mv	Zhang, Yinghao @@aut@@ Xu, Qiao @@aut@@ Wang, Feiyu @@aut@@ Gao, Tao @@aut@@ Wei, Tianxiang @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	306710358
dewey-sort	3530
id	ELV008789614
language_de	englisch
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author	Zhang, Yinghao
spellingShingle	Zhang, Yinghao ddc 530 bkl 50.22 bkl 35.07 misc Enzyme powered self-assembly misc Hydrogel biosensor misc Colorimetric detection of metabolites misc One-tube assay kit Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites
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topic_title	530 620 DE-600 50.22 bkl 35.07 bkl Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites Enzyme powered self-assembly Hydrogel biosensor Colorimetric detection of metabolites One-tube assay kit
topic	ddc 530 bkl 50.22 bkl 35.07 misc Enzyme powered self-assembly misc Hydrogel biosensor misc Colorimetric detection of metabolites misc One-tube assay kit
topic_unstemmed	ddc 530 bkl 50.22 bkl 35.07 misc Enzyme powered self-assembly misc Hydrogel biosensor misc Colorimetric detection of metabolites misc One-tube assay kit
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title	Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites
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title_full	Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites
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author_browse	Zhang, Yinghao Xu, Qiao Wang, Feiyu Gao, Tao Wei, Tianxiang
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title_sort	enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites
title_auth	Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites
abstract	Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatible for encapsulation of detection enzymes while retain their activities, so as to facilitate catalytic detection of specific metabolites with colorimetric signal readout. For convenience of the operation, the hydrogel biosensor has been further fitted in a test tube to construct a new kind of one-tube assay kit. For the proof-of-concept, assay kits for hydrogen peroxide (H2O2) and glucose have been testified. The linear detection ranges for H2O2 and glucose are 0.05–1.50 mM and 2–50 mM, the limit of detection (LOD) are 8.9 µM and 1.6 mM, and the entire assay time require only 1 min and 6 min, respectively. They exhibit high sensitivity and specificity both in test solution and real serum samples. Therefore, this one-tube colorimetric assay kit is promising for in home medical diagnosis and clinical routine rapid testing. Additionally, the hydrogel biosensor system may have universality for embedding other detecting enzymes, and enable many biomedical tests based on specific enzymes.
abstractGer	Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatible for encapsulation of detection enzymes while retain their activities, so as to facilitate catalytic detection of specific metabolites with colorimetric signal readout. For convenience of the operation, the hydrogel biosensor has been further fitted in a test tube to construct a new kind of one-tube assay kit. For the proof-of-concept, assay kits for hydrogen peroxide (H2O2) and glucose have been testified. The linear detection ranges for H2O2 and glucose are 0.05–1.50 mM and 2–50 mM, the limit of detection (LOD) are 8.9 µM and 1.6 mM, and the entire assay time require only 1 min and 6 min, respectively. They exhibit high sensitivity and specificity both in test solution and real serum samples. Therefore, this one-tube colorimetric assay kit is promising for in home medical diagnosis and clinical routine rapid testing. Additionally, the hydrogel biosensor system may have universality for embedding other detecting enzymes, and enable many biomedical tests based on specific enzymes.
abstract_unstemmed	Enzyme powered self-assembly strategy is proposed in this work to construct enzyme-entrapped hydrogel biosensor, for simple, rapid, and colorimetric detection of metabolites. Specifically, the enzyme itself drives the assembly of hydrogel biosensor. The gelation process powered by enzyme is compatible for encapsulation of detection enzymes while retain their activities, so as to facilitate catalytic detection of specific metabolites with colorimetric signal readout. For convenience of the operation, the hydrogel biosensor has been further fitted in a test tube to construct a new kind of one-tube assay kit. For the proof-of-concept, assay kits for hydrogen peroxide (H2O2) and glucose have been testified. The linear detection ranges for H2O2 and glucose are 0.05–1.50 mM and 2–50 mM, the limit of detection (LOD) are 8.9 µM and 1.6 mM, and the entire assay time require only 1 min and 6 min, respectively. They exhibit high sensitivity and specificity both in test solution and real serum samples. Therefore, this one-tube colorimetric assay kit is promising for in home medical diagnosis and clinical routine rapid testing. Additionally, the hydrogel biosensor system may have universality for embedding other detecting enzymes, and enable many biomedical tests based on specific enzymes.
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title_short	Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites
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author2	Xu, Qiao Wang, Feiyu Gao, Tao Wei, Tianxiang
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up_date	2024-07-06T20:54:36.722Z
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Enzyme powered self-assembly of hydrogel biosensor for colorimetric detection of metabolites

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