Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein
Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in chor...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Yu, Yan [verfasserIn] Peng, Qiaohua [verfasserIn] Zhao, Peng [verfasserIn] Wang, Lingfang [verfasserIn] Weng, Yang [verfasserIn] Chen, Xiaojing [verfasserIn] Li, Xingxia [verfasserIn] Feng, Suwen [verfasserIn] Wang, Xinyu [verfasserIn] Lu, Weiguo [verfasserIn] Xie, Xing [verfasserIn] Cheng, Xiaodong [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2022 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Placenta - Amsterdam [u.a.] : Elsevier, 1980, 130, Seite 46-52 |
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| Übergeordnetes Werk: |
volume:130 ; pages:46-52 |
| DOI / URN: |
10.1016/j.placenta.2022.10.015 |
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| Katalog-ID: |
ELV008873690 |
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| 024 | 7 | |a 10.1016/j.placenta.2022.10.015 |2 doi | |
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| 245 | 1 | 0 | |a Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein |
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| 520 | |a Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in choriocarcinoma.Methods: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-interfering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and invasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western blotting.Results: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-downregulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated α-tubulin.Discussion: Thus, MIIP—by inhibiting cellular motility in choriocarcinoma—acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acetylated α-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells. | ||
| 650 | 4 | |a Choriocarcinoma | |
| 650 | 4 | |a Migration and invasion inhibitory protein | |
| 650 | 4 | |a MIIP | |
| 650 | 4 | |a Invasion | |
| 650 | 4 | |a Metastasis | |
| 700 | 1 | |a Peng, Qiaohua |e verfasserin |4 aut | |
| 700 | 1 | |a Zhao, Peng |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Lingfang |e verfasserin |4 aut | |
| 700 | 1 | |a Weng, Yang |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Xiaojing |e verfasserin |4 aut | |
| 700 | 1 | |a Li, Xingxia |e verfasserin |4 aut | |
| 700 | 1 | |a Feng, Suwen |e verfasserin |4 aut | |
| 700 | 1 | |a Wang, Xinyu |e verfasserin |4 aut | |
| 700 | 1 | |a Lu, Weiguo |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Xing |e verfasserin |4 aut | |
| 700 | 1 | |a Cheng, Xiaodong |e verfasserin |0 (orcid)0000-0002-6073-7261 |4 aut | |
| 773 | 0 | 8 | |i Enthalten in |t Placenta |d Amsterdam [u.a.] : Elsevier, 1980 |g 130, Seite 46-52 |h Online-Ressource |w (DE-627)320420442 |w (DE-600)2002489-7 |w (DE-576)106869582 |x 1532-3102 |7 nnns |
| 773 | 1 | 8 | |g volume:130 |g pages:46-52 |
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10.1016/j.placenta.2022.10.015 doi (DE-627)ELV008873690 (ELSEVIER)S0143-4004(22)00440-4 DE-627 ger DE-627 rda eng 610 DE-600 44.92 bkl Yu, Yan verfasserin (orcid)0000-0001-5190-8869 aut Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in choriocarcinoma.Methods: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-interfering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and invasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western blotting.Results: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-downregulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated α-tubulin.Discussion: Thus, MIIP—by inhibiting cellular motility in choriocarcinoma—acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acetylated α-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells. Choriocarcinoma Migration and invasion inhibitory protein MIIP Invasion Metastasis Peng, Qiaohua verfasserin aut Zhao, Peng verfasserin aut Wang, Lingfang verfasserin aut Weng, Yang verfasserin aut Chen, Xiaojing verfasserin aut Li, Xingxia verfasserin aut Feng, Suwen verfasserin aut Wang, Xinyu verfasserin aut Lu, Weiguo verfasserin aut Xie, Xing verfasserin aut Cheng, Xiaodong verfasserin (orcid)0000-0002-6073-7261 aut Enthalten in Placenta Amsterdam [u.a.] : Elsevier, 1980 130, Seite 46-52 Online-Ressource (DE-627)320420442 (DE-600)2002489-7 (DE-576)106869582 1532-3102 nnns volume:130 pages:46-52 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.92 Gynäkologie AR 130 46-52 |
| spelling |
10.1016/j.placenta.2022.10.015 doi (DE-627)ELV008873690 (ELSEVIER)S0143-4004(22)00440-4 DE-627 ger DE-627 rda eng 610 DE-600 44.92 bkl Yu, Yan verfasserin (orcid)0000-0001-5190-8869 aut Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in choriocarcinoma.Methods: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-interfering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and invasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western blotting.Results: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-downregulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated α-tubulin.Discussion: Thus, MIIP—by inhibiting cellular motility in choriocarcinoma—acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acetylated α-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells. Choriocarcinoma Migration and invasion inhibitory protein MIIP Invasion Metastasis Peng, Qiaohua verfasserin aut Zhao, Peng verfasserin aut Wang, Lingfang verfasserin aut Weng, Yang verfasserin aut Chen, Xiaojing verfasserin aut Li, Xingxia verfasserin aut Feng, Suwen verfasserin aut Wang, Xinyu verfasserin aut Lu, Weiguo verfasserin aut Xie, Xing verfasserin aut Cheng, Xiaodong verfasserin (orcid)0000-0002-6073-7261 aut Enthalten in Placenta Amsterdam [u.a.] : Elsevier, 1980 130, Seite 46-52 Online-Ressource (DE-627)320420442 (DE-600)2002489-7 (DE-576)106869582 1532-3102 nnns volume:130 pages:46-52 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.92 Gynäkologie AR 130 46-52 |
| allfields_unstemmed |
10.1016/j.placenta.2022.10.015 doi (DE-627)ELV008873690 (ELSEVIER)S0143-4004(22)00440-4 DE-627 ger DE-627 rda eng 610 DE-600 44.92 bkl Yu, Yan verfasserin (orcid)0000-0001-5190-8869 aut Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in choriocarcinoma.Methods: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-interfering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and invasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western blotting.Results: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-downregulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated α-tubulin.Discussion: Thus, MIIP—by inhibiting cellular motility in choriocarcinoma—acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acetylated α-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells. Choriocarcinoma Migration and invasion inhibitory protein MIIP Invasion Metastasis Peng, Qiaohua verfasserin aut Zhao, Peng verfasserin aut Wang, Lingfang verfasserin aut Weng, Yang verfasserin aut Chen, Xiaojing verfasserin aut Li, Xingxia verfasserin aut Feng, Suwen verfasserin aut Wang, Xinyu verfasserin aut Lu, Weiguo verfasserin aut Xie, Xing verfasserin aut Cheng, Xiaodong verfasserin (orcid)0000-0002-6073-7261 aut Enthalten in Placenta Amsterdam [u.a.] : Elsevier, 1980 130, Seite 46-52 Online-Ressource (DE-627)320420442 (DE-600)2002489-7 (DE-576)106869582 1532-3102 nnns volume:130 pages:46-52 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.92 Gynäkologie AR 130 46-52 |
| allfieldsGer |
10.1016/j.placenta.2022.10.015 doi (DE-627)ELV008873690 (ELSEVIER)S0143-4004(22)00440-4 DE-627 ger DE-627 rda eng 610 DE-600 44.92 bkl Yu, Yan verfasserin (orcid)0000-0001-5190-8869 aut Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in choriocarcinoma.Methods: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-interfering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and invasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western blotting.Results: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-downregulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated α-tubulin.Discussion: Thus, MIIP—by inhibiting cellular motility in choriocarcinoma—acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acetylated α-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells. Choriocarcinoma Migration and invasion inhibitory protein MIIP Invasion Metastasis Peng, Qiaohua verfasserin aut Zhao, Peng verfasserin aut Wang, Lingfang verfasserin aut Weng, Yang verfasserin aut Chen, Xiaojing verfasserin aut Li, Xingxia verfasserin aut Feng, Suwen verfasserin aut Wang, Xinyu verfasserin aut Lu, Weiguo verfasserin aut Xie, Xing verfasserin aut Cheng, Xiaodong verfasserin (orcid)0000-0002-6073-7261 aut Enthalten in Placenta Amsterdam [u.a.] : Elsevier, 1980 130, Seite 46-52 Online-Ressource (DE-627)320420442 (DE-600)2002489-7 (DE-576)106869582 1532-3102 nnns volume:130 pages:46-52 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.92 Gynäkologie AR 130 46-52 |
| allfieldsSound |
10.1016/j.placenta.2022.10.015 doi (DE-627)ELV008873690 (ELSEVIER)S0143-4004(22)00440-4 DE-627 ger DE-627 rda eng 610 DE-600 44.92 bkl Yu, Yan verfasserin (orcid)0000-0001-5190-8869 aut Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in choriocarcinoma.Methods: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-interfering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and invasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western blotting.Results: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-downregulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated α-tubulin.Discussion: Thus, MIIP—by inhibiting cellular motility in choriocarcinoma—acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acetylated α-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells. Choriocarcinoma Migration and invasion inhibitory protein MIIP Invasion Metastasis Peng, Qiaohua verfasserin aut Zhao, Peng verfasserin aut Wang, Lingfang verfasserin aut Weng, Yang verfasserin aut Chen, Xiaojing verfasserin aut Li, Xingxia verfasserin aut Feng, Suwen verfasserin aut Wang, Xinyu verfasserin aut Lu, Weiguo verfasserin aut Xie, Xing verfasserin aut Cheng, Xiaodong verfasserin (orcid)0000-0002-6073-7261 aut Enthalten in Placenta Amsterdam [u.a.] : Elsevier, 1980 130, Seite 46-52 Online-Ressource (DE-627)320420442 (DE-600)2002489-7 (DE-576)106869582 1532-3102 nnns volume:130 pages:46-52 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.92 Gynäkologie AR 130 46-52 |
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Yu, Yan @@aut@@ Peng, Qiaohua @@aut@@ Zhao, Peng @@aut@@ Wang, Lingfang @@aut@@ Weng, Yang @@aut@@ Chen, Xiaojing @@aut@@ Li, Xingxia @@aut@@ Feng, Suwen @@aut@@ Wang, Xinyu @@aut@@ Lu, Weiguo @@aut@@ Xie, Xing @@aut@@ Cheng, Xiaodong @@aut@@ |
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2022-01-01T00:00:00Z |
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Yu, Yan |
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Yu, Yan ddc 610 bkl 44.92 misc Choriocarcinoma misc Migration and invasion inhibitory protein misc MIIP misc Invasion misc Metastasis Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein |
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610 DE-600 44.92 bkl Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein Choriocarcinoma Migration and invasion inhibitory protein MIIP Invasion Metastasis |
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Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein |
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Yu, Yan Peng, Qiaohua Zhao, Peng Wang, Lingfang Weng, Yang Chen, Xiaojing Li, Xingxia Feng, Suwen Wang, Xinyu Lu, Weiguo Xie, Xing Cheng, Xiaodong |
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inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein |
| title_auth |
Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein |
| abstract |
Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in choriocarcinoma.Methods: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-interfering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and invasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western blotting.Results: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-downregulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated α-tubulin.Discussion: Thus, MIIP—by inhibiting cellular motility in choriocarcinoma—acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acetylated α-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells. |
| abstractGer |
Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in choriocarcinoma.Methods: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-interfering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and invasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western blotting.Results: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-downregulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated α-tubulin.Discussion: Thus, MIIP—by inhibiting cellular motility in choriocarcinoma—acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acetylated α-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells. |
| abstract_unstemmed |
Introduction: Choriocarcinoma is a highly invasive gynaecologic malignancy. Molecular mechanism of metastasis in choriocarcinoma is poorly understood. Migration and invasion inhibitory protein (MIIP) regulates cell migration and invasion. Therefore, we aimed to elucidate the function of MIIP in choriocarcinoma.Methods: Choriocarcinoma cell lines, JAR and JEG-3, were transfected with lentivirus carrying the MIIP-interfering RNA (to downregulate MIIP expression) or left untransfected (negative control). Cell migration and invasion were studied using transwell migration assays and scratch assays. In vivo tumour burden was studied using tumour xenograft models in specific-pathogen-free nude mice and live imaging. We elucidated possible molecular signalling pathways using western blotting.Results: In transwell migration and scratch assays MIIP-downregulated JAR and JEG-3 cells migrated and invaded faster compared to their respective negative control cells. Migration and invasion by the MIIP-upregulated SWAN cells was slower than that by negative control SWAN cells. Live imaging revealed that bioluminescence values were higher in MIIP-downregulated tumours than in the negative control tumours. Mice with MIIP-downregulated tumours had higher serum human chorionic gonadotropin (HCG) levels than those with negative control tumours. The MIIP expression was negatively correlated with that of histone deacetylase (HDAC6) and positively correlated with that of acetylated α-tubulin.Discussion: Thus, MIIP—by inhibiting cellular motility in choriocarcinoma—acts as a tumour suppressor gene. This highlights a potential therapeutic target for refractory choriocarcinoma. Additionally, HDAC6 and acetylated α-tubulin may be involved in the regulatory effects of MIIP on the biobehaviour of choriocarcinoma cells. |
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| title_short |
Inhibition of invasion and metastasis in choriocarcinoma by migration and invasion inhibitory protein |
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Peng, Qiaohua Zhao, Peng Wang, Lingfang Weng, Yang Chen, Xiaojing Li, Xingxia Feng, Suwen Wang, Xinyu Lu, Weiguo Xie, Xing Cheng, Xiaodong |
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7.40158 |