Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain

Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been...
Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Cerda-Peña, Carol [verfasserIn] Contreras, Sergio [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Vegetation Leaf habit Biomarker Deciduous Evergreen

Übergeordnetes Werk:	Enthalten in: Ecological indicators - Amsterdam [u.a.] : Elsevier Science, 2001, 136
Übergeordnetes Werk:	volume:136

DOI / URN:	10.1016/j.ecolind.2022.108675

Katalog-ID:	ELV009754369

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520			\|a Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been studied. The main goal of this research was to characterize twelve dominant modern species of the STF using three classes of leaf wax compounds, n-alkanoic acids, n-alkanes and n-alkanols. In addition, we evaluate the potential of leaf waxes as a vegetation and chemotaxonomic biomarker in the region, considering species that were found in different sampling sites and therefore environmental conditions. Clear differences among leaf wax abundance (μg/g) and ACL (average chain length) within and among the twelve species were found. Only the ACL of n-alkanoic acids and n-alkanols allows differentiation between leaf habit species (i.e. evergreen vs. deciduous), with high values associated with evergreen and low values with deciduous plants. This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. The clear chemotaxonomic differences among the five species exposed to different and natural environmental conditions and the high preservation potential of the study area allow us to suggest that leaf waxes are likely a reliable tool to be incorporated in quantitative models to track vegetation and may be useful as a chemotaxonomic biomarker at the species level.
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publishDate	2022
allfields	10.1016/j.ecolind.2022.108675 doi (DE-627)ELV009754369 (ELSEVIER)S1470-160X(22)00146-7 DE-627 ger DE-627 rda eng 570 630 VZ BIODIV DE-30 fid Cerda-Peña, Carol verfasserin aut Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been studied. The main goal of this research was to characterize twelve dominant modern species of the STF using three classes of leaf wax compounds, n-alkanoic acids, n-alkanes and n-alkanols. In addition, we evaluate the potential of leaf waxes as a vegetation and chemotaxonomic biomarker in the region, considering species that were found in different sampling sites and therefore environmental conditions. Clear differences among leaf wax abundance (μg/g) and ACL (average chain length) within and among the twelve species were found. Only the ACL of n-alkanoic acids and n-alkanols allows differentiation between leaf habit species (i.e. evergreen vs. deciduous), with high values associated with evergreen and low values with deciduous plants. This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. The clear chemotaxonomic differences among the five species exposed to different and natural environmental conditions and the high preservation potential of the study area allow us to suggest that leaf waxes are likely a reliable tool to be incorporated in quantitative models to track vegetation and may be useful as a chemotaxonomic biomarker at the species level. Vegetation Leaf habit Biomarker Deciduous Evergreen Contreras, Sergio verfasserin aut Enthalten in Ecological indicators Amsterdam [u.a.] : Elsevier Science, 2001 136 Online-Ressource (DE-627)338074163 (DE-600)2063587-4 (DE-576)259272388 1872-7034 nnns volume:136 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 136
spelling	10.1016/j.ecolind.2022.108675 doi (DE-627)ELV009754369 (ELSEVIER)S1470-160X(22)00146-7 DE-627 ger DE-627 rda eng 570 630 VZ BIODIV DE-30 fid Cerda-Peña, Carol verfasserin aut Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been studied. The main goal of this research was to characterize twelve dominant modern species of the STF using three classes of leaf wax compounds, n-alkanoic acids, n-alkanes and n-alkanols. In addition, we evaluate the potential of leaf waxes as a vegetation and chemotaxonomic biomarker in the region, considering species that were found in different sampling sites and therefore environmental conditions. Clear differences among leaf wax abundance (μg/g) and ACL (average chain length) within and among the twelve species were found. Only the ACL of n-alkanoic acids and n-alkanols allows differentiation between leaf habit species (i.e. evergreen vs. deciduous), with high values associated with evergreen and low values with deciduous plants. This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. The clear chemotaxonomic differences among the five species exposed to different and natural environmental conditions and the high preservation potential of the study area allow us to suggest that leaf waxes are likely a reliable tool to be incorporated in quantitative models to track vegetation and may be useful as a chemotaxonomic biomarker at the species level. Vegetation Leaf habit Biomarker Deciduous Evergreen Contreras, Sergio verfasserin aut Enthalten in Ecological indicators Amsterdam [u.a.] : Elsevier Science, 2001 136 Online-Ressource (DE-627)338074163 (DE-600)2063587-4 (DE-576)259272388 1872-7034 nnns volume:136 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 136
allfields_unstemmed	10.1016/j.ecolind.2022.108675 doi (DE-627)ELV009754369 (ELSEVIER)S1470-160X(22)00146-7 DE-627 ger DE-627 rda eng 570 630 VZ BIODIV DE-30 fid Cerda-Peña, Carol verfasserin aut Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been studied. The main goal of this research was to characterize twelve dominant modern species of the STF using three classes of leaf wax compounds, n-alkanoic acids, n-alkanes and n-alkanols. In addition, we evaluate the potential of leaf waxes as a vegetation and chemotaxonomic biomarker in the region, considering species that were found in different sampling sites and therefore environmental conditions. Clear differences among leaf wax abundance (μg/g) and ACL (average chain length) within and among the twelve species were found. Only the ACL of n-alkanoic acids and n-alkanols allows differentiation between leaf habit species (i.e. evergreen vs. deciduous), with high values associated with evergreen and low values with deciduous plants. This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. The clear chemotaxonomic differences among the five species exposed to different and natural environmental conditions and the high preservation potential of the study area allow us to suggest that leaf waxes are likely a reliable tool to be incorporated in quantitative models to track vegetation and may be useful as a chemotaxonomic biomarker at the species level. Vegetation Leaf habit Biomarker Deciduous Evergreen Contreras, Sergio verfasserin aut Enthalten in Ecological indicators Amsterdam [u.a.] : Elsevier Science, 2001 136 Online-Ressource (DE-627)338074163 (DE-600)2063587-4 (DE-576)259272388 1872-7034 nnns volume:136 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 136
allfieldsGer	10.1016/j.ecolind.2022.108675 doi (DE-627)ELV009754369 (ELSEVIER)S1470-160X(22)00146-7 DE-627 ger DE-627 rda eng 570 630 VZ BIODIV DE-30 fid Cerda-Peña, Carol verfasserin aut Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been studied. The main goal of this research was to characterize twelve dominant modern species of the STF using three classes of leaf wax compounds, n-alkanoic acids, n-alkanes and n-alkanols. In addition, we evaluate the potential of leaf waxes as a vegetation and chemotaxonomic biomarker in the region, considering species that were found in different sampling sites and therefore environmental conditions. Clear differences among leaf wax abundance (μg/g) and ACL (average chain length) within and among the twelve species were found. Only the ACL of n-alkanoic acids and n-alkanols allows differentiation between leaf habit species (i.e. evergreen vs. deciduous), with high values associated with evergreen and low values with deciduous plants. This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. The clear chemotaxonomic differences among the five species exposed to different and natural environmental conditions and the high preservation potential of the study area allow us to suggest that leaf waxes are likely a reliable tool to be incorporated in quantitative models to track vegetation and may be useful as a chemotaxonomic biomarker at the species level. Vegetation Leaf habit Biomarker Deciduous Evergreen Contreras, Sergio verfasserin aut Enthalten in Ecological indicators Amsterdam [u.a.] : Elsevier Science, 2001 136 Online-Ressource (DE-627)338074163 (DE-600)2063587-4 (DE-576)259272388 1872-7034 nnns volume:136 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 136
allfieldsSound	10.1016/j.ecolind.2022.108675 doi (DE-627)ELV009754369 (ELSEVIER)S1470-160X(22)00146-7 DE-627 ger DE-627 rda eng 570 630 VZ BIODIV DE-30 fid Cerda-Peña, Carol verfasserin aut Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been studied. The main goal of this research was to characterize twelve dominant modern species of the STF using three classes of leaf wax compounds, n-alkanoic acids, n-alkanes and n-alkanols. In addition, we evaluate the potential of leaf waxes as a vegetation and chemotaxonomic biomarker in the region, considering species that were found in different sampling sites and therefore environmental conditions. Clear differences among leaf wax abundance (μg/g) and ACL (average chain length) within and among the twelve species were found. Only the ACL of n-alkanoic acids and n-alkanols allows differentiation between leaf habit species (i.e. evergreen vs. deciduous), with high values associated with evergreen and low values with deciduous plants. This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. The clear chemotaxonomic differences among the five species exposed to different and natural environmental conditions and the high preservation potential of the study area allow us to suggest that leaf waxes are likely a reliable tool to be incorporated in quantitative models to track vegetation and may be useful as a chemotaxonomic biomarker at the species level. Vegetation Leaf habit Biomarker Deciduous Evergreen Contreras, Sergio verfasserin aut Enthalten in Ecological indicators Amsterdam [u.a.] : Elsevier Science, 2001 136 Online-Ressource (DE-627)338074163 (DE-600)2063587-4 (DE-576)259272388 1872-7034 nnns volume:136 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2025 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 AR 136
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author	Cerda-Peña, Carol
spellingShingle	Cerda-Peña, Carol ddc 570 fid BIODIV misc Vegetation misc Leaf habit misc Biomarker misc Deciduous misc Evergreen Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain
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topic_title	570 630 VZ BIODIV DE-30 fid Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain Vegetation Leaf habit Biomarker Deciduous Evergreen
topic	ddc 570 fid BIODIV misc Vegetation misc Leaf habit misc Biomarker misc Deciduous misc Evergreen
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title	Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain
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title_full	Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain
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title_sort	characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain
title_auth	Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain
abstract	Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been studied. The main goal of this research was to characterize twelve dominant modern species of the STF using three classes of leaf wax compounds, n-alkanoic acids, n-alkanes and n-alkanols. In addition, we evaluate the potential of leaf waxes as a vegetation and chemotaxonomic biomarker in the region, considering species that were found in different sampling sites and therefore environmental conditions. Clear differences among leaf wax abundance (μg/g) and ACL (average chain length) within and among the twelve species were found. Only the ACL of n-alkanoic acids and n-alkanols allows differentiation between leaf habit species (i.e. evergreen vs. deciduous), with high values associated with evergreen and low values with deciduous plants. This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. The clear chemotaxonomic differences among the five species exposed to different and natural environmental conditions and the high preservation potential of the study area allow us to suggest that leaf waxes are likely a reliable tool to be incorporated in quantitative models to track vegetation and may be useful as a chemotaxonomic biomarker at the species level.
abstractGer	Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been studied. The main goal of this research was to characterize twelve dominant modern species of the STF using three classes of leaf wax compounds, n-alkanoic acids, n-alkanes and n-alkanols. In addition, we evaluate the potential of leaf waxes as a vegetation and chemotaxonomic biomarker in the region, considering species that were found in different sampling sites and therefore environmental conditions. Clear differences among leaf wax abundance (μg/g) and ACL (average chain length) within and among the twelve species were found. Only the ACL of n-alkanoic acids and n-alkanols allows differentiation between leaf habit species (i.e. evergreen vs. deciduous), with high values associated with evergreen and low values with deciduous plants. This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. The clear chemotaxonomic differences among the five species exposed to different and natural environmental conditions and the high preservation potential of the study area allow us to suggest that leaf waxes are likely a reliable tool to be incorporated in quantitative models to track vegetation and may be useful as a chemotaxonomic biomarker at the species level.
abstract_unstemmed	Plant leaf waxes are used as vegetation biomarkers in several archives (i.e. soils, lake and marine sediments), study of these compounds in modern plants is needed to makes their application and interpretation more robust. However, in the South American Temperate Forest (STF), few species have been studied. The main goal of this research was to characterize twelve dominant modern species of the STF using three classes of leaf wax compounds, n-alkanoic acids, n-alkanes and n-alkanols. In addition, we evaluate the potential of leaf waxes as a vegetation and chemotaxonomic biomarker in the region, considering species that were found in different sampling sites and therefore environmental conditions. Clear differences among leaf wax abundance (μg/g) and ACL (average chain length) within and among the twelve species were found. Only the ACL of n-alkanoic acids and n-alkanols allows differentiation between leaf habit species (i.e. evergreen vs. deciduous), with high values associated with evergreen and low values with deciduous plants. This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. The clear chemotaxonomic differences among the five species exposed to different and natural environmental conditions and the high preservation potential of the study area allow us to suggest that leaf waxes are likely a reliable tool to be incorporated in quantitative models to track vegetation and may be useful as a chemotaxonomic biomarker at the species level.
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title_short	Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain
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This study differentiates the five species found in more than one site (i.e. different environmental condition) using different combinations of leaf waxes and in addition using only n-alkanes. It was not possible to differentiate among all sites with any combination of leaf waxes. The differences in the distribution of leaf waxes among species is an expected pattern in the study area, and it seems reliable to use the ACL as a vegetation biomarker differentiating between evergreen and deciduous species. 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Characterization and chemo-taxonomic evaluation of plant leaf waxes (long chain

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