Bioproduction, purification and physicochemical characterization of melanin from

Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain M...
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Gespeichert in:

Autor*in:	Rudrappa, Muthuraj [verfasserIn] Kumar M, Santosh [verfasserIn] Kumar, Raju Suresh [verfasserIn] Almansour, Abdulrahman I. [verfasserIn] Perumal, Karthikeyan [verfasserIn] Nayaka, Sreenivasa [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2022

Schlagwörter:	Melanin Tyrosinase Microbial pigment

Übergeordnetes Werk:	Enthalten in: Microbiological research - Jena : Urban & Fischer, 1994, 263
Übergeordnetes Werk:	volume:263

DOI / URN:	10.1016/j.micres.2022.127130

Katalog-ID:	ELV009833730

Internformat


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100	1		\|a Rudrappa, Muthuraj \|e verfasserin \|4 aut
245	1	0	\|a Bioproduction, purification and physicochemical characterization of melanin from
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520			\|a Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field.
650		4	\|a Melanin
650		4	\|a Tyrosinase
650		4	\|a Microbial pigment
700	1		\|a Kumar M, Santosh \|e verfasserin \|4 aut
700	1		\|a Kumar, Raju Suresh \|e verfasserin \|4 aut
700	1		\|a Almansour, Abdulrahman I. \|e verfasserin \|4 aut
700	1		\|a Perumal, Karthikeyan \|e verfasserin \|4 aut
700	1		\|a Nayaka, Sreenivasa \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Microbiological research \|d Jena : Urban & Fischer, 1994 \|g 263 \|h Online-Ressource \|w (DE-627)331504820 \|w (DE-600)2051526-1 \|w (DE-576)094481032 \|x 1618-0623 \|7 nnns
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912			\|a GBV_ILN_151
912			\|a GBV_ILN_161
912			\|a GBV_ILN_170
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_285
912			\|a GBV_ILN_293
912			\|a GBV_ILN_370
912			\|a GBV_ILN_381
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
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912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
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912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
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912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
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912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4012
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
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publishDate	2022
allfields	10.1016/j.micres.2022.127130 doi (DE-627)ELV009833730 (ELSEVIER)S0944-5013(22)00170-7 DE-627 ger DE-627 rda eng 570 VZ BIODIV DE-30 fid 42.30 bkl Rudrappa, Muthuraj verfasserin aut Bioproduction, purification and physicochemical characterization of melanin from 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field. Melanin Tyrosinase Microbial pigment Kumar M, Santosh verfasserin aut Kumar, Raju Suresh verfasserin aut Almansour, Abdulrahman I. verfasserin aut Perumal, Karthikeyan verfasserin aut Nayaka, Sreenivasa verfasserin aut Enthalten in Microbiological research Jena : Urban & Fischer, 1994 263 Online-Ressource (DE-627)331504820 (DE-600)2051526-1 (DE-576)094481032 1618-0623 nnns volume:263 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 Mikrobiologie VZ AR 263
spelling	10.1016/j.micres.2022.127130 doi (DE-627)ELV009833730 (ELSEVIER)S0944-5013(22)00170-7 DE-627 ger DE-627 rda eng 570 VZ BIODIV DE-30 fid 42.30 bkl Rudrappa, Muthuraj verfasserin aut Bioproduction, purification and physicochemical characterization of melanin from 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field. Melanin Tyrosinase Microbial pigment Kumar M, Santosh verfasserin aut Kumar, Raju Suresh verfasserin aut Almansour, Abdulrahman I. verfasserin aut Perumal, Karthikeyan verfasserin aut Nayaka, Sreenivasa verfasserin aut Enthalten in Microbiological research Jena : Urban & Fischer, 1994 263 Online-Ressource (DE-627)331504820 (DE-600)2051526-1 (DE-576)094481032 1618-0623 nnns volume:263 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 Mikrobiologie VZ AR 263
allfields_unstemmed	10.1016/j.micres.2022.127130 doi (DE-627)ELV009833730 (ELSEVIER)S0944-5013(22)00170-7 DE-627 ger DE-627 rda eng 570 VZ BIODIV DE-30 fid 42.30 bkl Rudrappa, Muthuraj verfasserin aut Bioproduction, purification and physicochemical characterization of melanin from 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field. Melanin Tyrosinase Microbial pigment Kumar M, Santosh verfasserin aut Kumar, Raju Suresh verfasserin aut Almansour, Abdulrahman I. verfasserin aut Perumal, Karthikeyan verfasserin aut Nayaka, Sreenivasa verfasserin aut Enthalten in Microbiological research Jena : Urban & Fischer, 1994 263 Online-Ressource (DE-627)331504820 (DE-600)2051526-1 (DE-576)094481032 1618-0623 nnns volume:263 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 Mikrobiologie VZ AR 263
allfieldsGer	10.1016/j.micres.2022.127130 doi (DE-627)ELV009833730 (ELSEVIER)S0944-5013(22)00170-7 DE-627 ger DE-627 rda eng 570 VZ BIODIV DE-30 fid 42.30 bkl Rudrappa, Muthuraj verfasserin aut Bioproduction, purification and physicochemical characterization of melanin from 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field. Melanin Tyrosinase Microbial pigment Kumar M, Santosh verfasserin aut Kumar, Raju Suresh verfasserin aut Almansour, Abdulrahman I. verfasserin aut Perumal, Karthikeyan verfasserin aut Nayaka, Sreenivasa verfasserin aut Enthalten in Microbiological research Jena : Urban & Fischer, 1994 263 Online-Ressource (DE-627)331504820 (DE-600)2051526-1 (DE-576)094481032 1618-0623 nnns volume:263 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 Mikrobiologie VZ AR 263
allfieldsSound	10.1016/j.micres.2022.127130 doi (DE-627)ELV009833730 (ELSEVIER)S0944-5013(22)00170-7 DE-627 ger DE-627 rda eng 570 VZ BIODIV DE-30 fid 42.30 bkl Rudrappa, Muthuraj verfasserin aut Bioproduction, purification and physicochemical characterization of melanin from 2022 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field. Melanin Tyrosinase Microbial pigment Kumar M, Santosh verfasserin aut Kumar, Raju Suresh verfasserin aut Almansour, Abdulrahman I. verfasserin aut Perumal, Karthikeyan verfasserin aut Nayaka, Sreenivasa verfasserin aut Enthalten in Microbiological research Jena : Urban & Fischer, 1994 263 Online-Ressource (DE-627)331504820 (DE-600)2051526-1 (DE-576)094481032 1618-0623 nnns volume:263 GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_381 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4012 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4393 GBV_ILN_4700 42.30 Mikrobiologie VZ AR 263
language	English
source	Enthalten in Microbiological research 263 volume:263
sourceStr	Enthalten in Microbiological research 263 volume:263
format_phy_str_mv	Article
bklname	Mikrobiologie
institution	findex.gbv.de
topic_facet	Melanin Tyrosinase Microbial pigment
dewey-raw	570
isfreeaccess_bool	false
container_title	Microbiological research
authorswithroles_txt_mv	Rudrappa, Muthuraj @@aut@@ Kumar M, Santosh @@aut@@ Kumar, Raju Suresh @@aut@@ Almansour, Abdulrahman I. @@aut@@ Perumal, Karthikeyan @@aut@@ Nayaka, Sreenivasa @@aut@@
publishDateDaySort_date	2022-01-01T00:00:00Z
hierarchy_top_id	331504820
dewey-sort	3570
id	ELV009833730
language_de	englisch
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TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. 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author	Rudrappa, Muthuraj
spellingShingle	Rudrappa, Muthuraj ddc 570 fid BIODIV bkl 42.30 misc Melanin misc Tyrosinase misc Microbial pigment Bioproduction, purification and physicochemical characterization of melanin from
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topic_title	570 VZ BIODIV DE-30 fid 42.30 bkl Bioproduction, purification and physicochemical characterization of melanin from Melanin Tyrosinase Microbial pigment
topic	ddc 570 fid BIODIV bkl 42.30 misc Melanin misc Tyrosinase misc Microbial pigment
topic_unstemmed	ddc 570 fid BIODIV bkl 42.30 misc Melanin misc Tyrosinase misc Microbial pigment
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title	Bioproduction, purification and physicochemical characterization of melanin from
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title_full	Bioproduction, purification and physicochemical characterization of melanin from
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author_browse	Rudrappa, Muthuraj Kumar M, Santosh Kumar, Raju Suresh Almansour, Abdulrahman I. Perumal, Karthikeyan Nayaka, Sreenivasa
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title_sort	bioproduction, purification and physicochemical characterization of melanin from
title_auth	Bioproduction, purification and physicochemical characterization of melanin from
abstract	Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field.
abstractGer	Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field.
abstract_unstemmed	Melanin has been produced and extracted from various microorganisms because of its therapeutic nature and diverse applications in various fields. Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field.
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title_short	Bioproduction, purification and physicochemical characterization of melanin from
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author2	Kumar M, Santosh Kumar, Raju Suresh Almansour, Abdulrahman I. Perumal, Karthikeyan Nayaka, Sreenivasa
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Hence we isolated actinomycetes from soil which is capable of producing melanin pigment from L-tyrosine and it was identified as Streptomyces sp. strain MR28 on the basis of biochemical, morphological characterization, and 16S rRNA gene sequencing. Production of melanin pigment was achieved by using standardized tyrosine broth. The melanin pigment was purified, and characterized by using various techniques such as Ultraviolet-Visible spectroscopy (UV-Vis), Fourier Transform Infrared spectroscopy (FTIR), Thin Layer Chromatography (TLC), 1H NMR spectroscopy, Scanning Electron Microscopy (SEM), Elemental analysis (EDX), and Thermogravimetric analysis (TGA). The pigment exhibit maximum UV-Vis absorption spectrum at 299 nm, FTIR peaks confirm the occurrence of C-H, C-N, C-O, and CC functional groups which are key functional groups in indole/pyrrole structure. TLC analysis showed a single band with a significant Retardation factor (Rf) of 0.68, Resonance peaks at 6.66, 7.18, and 7.28 ppm exhibit aromatic hydrogen in the indole/pyrole system in 1H NMR. The EDX reports the presence of carbon, nitrogen, oxygen, and sulfur which are key elements in melanin structure, and TGA exhibits the thermal stability of the melanin. Overall, the successful production and extraction of melanin was achieved by using soil actinomycetes Streptomyces sp. strain MR28, and its characterization confirms the nature of the melanin pigment which has significant value in the industrial and biomedical field.</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Melanin</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Tyrosinase</subfield></datafield><datafield tag="650" ind1=" " ind2="4"><subfield code="a">Microbial pigment</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kumar M, Santosh</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Kumar, Raju Suresh</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Almansour, Abdulrahman 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