A phage cocktail in controlling phage resistance development in multidrug resistant

Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continu...
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Autor*in:	Yu, Huabo [verfasserIn] Zhang, Liang [verfasserIn] Feng, Chao [verfasserIn] Chi, Teng [verfasserIn] Qi, Yanling [verfasserIn] Abbas Raza, Sayed Haidar [verfasserIn] Gao, Na [verfasserIn] Jia, Kaixiang [verfasserIn] Zhang, Yang [verfasserIn] Fan, Ruining [verfasserIn] Cai, Ruopeng [verfasserIn] Qian, Aidong [verfasserIn] Li, Ying [verfasserIn] Sun, Wuwen [verfasserIn] Shan, Xiaofeng [verfasserIn] Liu, Ning [verfasserIn] Zhang, Lei [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2021

Schlagwörter:	Bacteriophage Phage cocktail therapy Phage resistant mutants

Übergeordnetes Werk:	Enthalten in: Microbial pathogenesis - Amsterdam [u.a.] : Elsevier, 1986, 162
Übergeordnetes Werk:	volume:162

DOI / URN:	10.1016/j.micpath.2021.105374

Katalog-ID:	ELV009873600

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245	1	0	\|a A phage cocktail in controlling phage resistance development in multidrug resistant
264		1	\|c 2021
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520			\|a Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). Phage therapy in vivo showed that the survival rate in the mixture phage treatment group was significantly higher than that in single phage treatment group.
650		4	\|a Bacteriophage
650		4	\|a Phage cocktail therapy
650		4	\|a Phage resistant mutants
700	1		\|a Zhang, Liang \|e verfasserin \|4 aut
700	1		\|a Feng, Chao \|e verfasserin \|4 aut
700	1		\|a Chi, Teng \|e verfasserin \|4 aut
700	1		\|a Qi, Yanling \|e verfasserin \|4 aut
700	1		\|a Abbas Raza, Sayed Haidar \|e verfasserin \|4 aut
700	1		\|a Gao, Na \|e verfasserin \|4 aut
700	1		\|a Jia, Kaixiang \|e verfasserin \|4 aut
700	1		\|a Zhang, Yang \|e verfasserin \|4 aut
700	1		\|a Fan, Ruining \|e verfasserin \|4 aut
700	1		\|a Cai, Ruopeng \|e verfasserin \|4 aut
700	1		\|a Qian, Aidong \|e verfasserin \|4 aut
700	1		\|a Li, Ying \|e verfasserin \|4 aut
700	1		\|a Sun, Wuwen \|e verfasserin \|4 aut
700	1		\|a Shan, Xiaofeng \|e verfasserin \|4 aut
700	1		\|a Liu, Ning \|e verfasserin \|4 aut
700	1		\|a Zhang, Lei \|e verfasserin \|0 (orcid)0000-0002-1869-5096 \|4 aut
773	0	8	\|i Enthalten in \|t Microbial pathogenesis \|d Amsterdam [u.a.] : Elsevier, 1986 \|g 162 \|h Online-Ressource \|w (DE-627)267840144 \|w (DE-600)1471158-8 \|w (DE-576)121465195 \|x 1096-1208 \|7 nnns
773	1	8	\|g volume:162
912			\|a GBV_USEFLAG_U
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912			\|a GBV_ELV
912			\|a SSG-OLC-PHA
912			\|a GBV_ILN_20
912			\|a GBV_ILN_22
912			\|a GBV_ILN_23
912			\|a GBV_ILN_24
912			\|a GBV_ILN_31
912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_63
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_224
912			\|a GBV_ILN_252
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2038
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2065
912			\|a GBV_ILN_2068
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2113
912			\|a GBV_ILN_2118
912			\|a GBV_ILN_2122
912			\|a GBV_ILN_2129
912			\|a GBV_ILN_2143
912			\|a GBV_ILN_2147
912			\|a GBV_ILN_2148
912			\|a GBV_ILN_2152
912			\|a GBV_ILN_2153
912			\|a GBV_ILN_2190
912			\|a GBV_ILN_2336
912			\|a GBV_ILN_2507
912			\|a GBV_ILN_2522
912			\|a GBV_ILN_4035
912			\|a GBV_ILN_4037
912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4126
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4335
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
936	b	k	\|a 44.43 \|j Medizinische Mikrobiologie \|q VZ
936	b	k	\|a 44.75 \|j Infektionskrankheiten \|j parasitäre Krankheiten \|x Medizin \|q VZ
951			\|a AR
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author_variant	h y hy l z lz c f cf t c tc y q yq r s h a rsh rsha n g ng k j kj y z yz r f rf r c rc a q aq y l yl w s ws x s xs n l nl l z lz
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hierarchy_sort_str	2021
bklnumber	44.43 44.75
publishDate	2021
allfields	10.1016/j.micpath.2021.105374 doi (DE-627)ELV009873600 (ELSEVIER)S0882-4010(21)00648-3 DE-627 ger DE-627 rda eng 610 VZ 44.43 bkl 44.75 bkl Yu, Huabo verfasserin aut A phage cocktail in controlling phage resistance development in multidrug resistant 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). Phage therapy in vivo showed that the survival rate in the mixture phage treatment group was significantly higher than that in single phage treatment group. Bacteriophage Phage cocktail therapy Phage resistant mutants Zhang, Liang verfasserin aut Feng, Chao verfasserin aut Chi, Teng verfasserin aut Qi, Yanling verfasserin aut Abbas Raza, Sayed Haidar verfasserin aut Gao, Na verfasserin aut Jia, Kaixiang verfasserin aut Zhang, Yang verfasserin aut Fan, Ruining verfasserin aut Cai, Ruopeng verfasserin aut Qian, Aidong verfasserin aut Li, Ying verfasserin aut Sun, Wuwen verfasserin aut Shan, Xiaofeng verfasserin aut Liu, Ning verfasserin aut Zhang, Lei verfasserin (orcid)0000-0002-1869-5096 aut Enthalten in Microbial pathogenesis Amsterdam [u.a.] : Elsevier, 1986 162 Online-Ressource (DE-627)267840144 (DE-600)1471158-8 (DE-576)121465195 1096-1208 nnns volume:162 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.43 Medizinische Mikrobiologie VZ 44.75 Infektionskrankheiten parasitäre Krankheiten Medizin VZ AR 162
spelling	10.1016/j.micpath.2021.105374 doi (DE-627)ELV009873600 (ELSEVIER)S0882-4010(21)00648-3 DE-627 ger DE-627 rda eng 610 VZ 44.43 bkl 44.75 bkl Yu, Huabo verfasserin aut A phage cocktail in controlling phage resistance development in multidrug resistant 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). Phage therapy in vivo showed that the survival rate in the mixture phage treatment group was significantly higher than that in single phage treatment group. Bacteriophage Phage cocktail therapy Phage resistant mutants Zhang, Liang verfasserin aut Feng, Chao verfasserin aut Chi, Teng verfasserin aut Qi, Yanling verfasserin aut Abbas Raza, Sayed Haidar verfasserin aut Gao, Na verfasserin aut Jia, Kaixiang verfasserin aut Zhang, Yang verfasserin aut Fan, Ruining verfasserin aut Cai, Ruopeng verfasserin aut Qian, Aidong verfasserin aut Li, Ying verfasserin aut Sun, Wuwen verfasserin aut Shan, Xiaofeng verfasserin aut Liu, Ning verfasserin aut Zhang, Lei verfasserin (orcid)0000-0002-1869-5096 aut Enthalten in Microbial pathogenesis Amsterdam [u.a.] : Elsevier, 1986 162 Online-Ressource (DE-627)267840144 (DE-600)1471158-8 (DE-576)121465195 1096-1208 nnns volume:162 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.43 Medizinische Mikrobiologie VZ 44.75 Infektionskrankheiten parasitäre Krankheiten Medizin VZ AR 162
allfields_unstemmed	10.1016/j.micpath.2021.105374 doi (DE-627)ELV009873600 (ELSEVIER)S0882-4010(21)00648-3 DE-627 ger DE-627 rda eng 610 VZ 44.43 bkl 44.75 bkl Yu, Huabo verfasserin aut A phage cocktail in controlling phage resistance development in multidrug resistant 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). Phage therapy in vivo showed that the survival rate in the mixture phage treatment group was significantly higher than that in single phage treatment group. Bacteriophage Phage cocktail therapy Phage resistant mutants Zhang, Liang verfasserin aut Feng, Chao verfasserin aut Chi, Teng verfasserin aut Qi, Yanling verfasserin aut Abbas Raza, Sayed Haidar verfasserin aut Gao, Na verfasserin aut Jia, Kaixiang verfasserin aut Zhang, Yang verfasserin aut Fan, Ruining verfasserin aut Cai, Ruopeng verfasserin aut Qian, Aidong verfasserin aut Li, Ying verfasserin aut Sun, Wuwen verfasserin aut Shan, Xiaofeng verfasserin aut Liu, Ning verfasserin aut Zhang, Lei verfasserin (orcid)0000-0002-1869-5096 aut Enthalten in Microbial pathogenesis Amsterdam [u.a.] : Elsevier, 1986 162 Online-Ressource (DE-627)267840144 (DE-600)1471158-8 (DE-576)121465195 1096-1208 nnns volume:162 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.43 Medizinische Mikrobiologie VZ 44.75 Infektionskrankheiten parasitäre Krankheiten Medizin VZ AR 162
allfieldsGer	10.1016/j.micpath.2021.105374 doi (DE-627)ELV009873600 (ELSEVIER)S0882-4010(21)00648-3 DE-627 ger DE-627 rda eng 610 VZ 44.43 bkl 44.75 bkl Yu, Huabo verfasserin aut A phage cocktail in controlling phage resistance development in multidrug resistant 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). Phage therapy in vivo showed that the survival rate in the mixture phage treatment group was significantly higher than that in single phage treatment group. Bacteriophage Phage cocktail therapy Phage resistant mutants Zhang, Liang verfasserin aut Feng, Chao verfasserin aut Chi, Teng verfasserin aut Qi, Yanling verfasserin aut Abbas Raza, Sayed Haidar verfasserin aut Gao, Na verfasserin aut Jia, Kaixiang verfasserin aut Zhang, Yang verfasserin aut Fan, Ruining verfasserin aut Cai, Ruopeng verfasserin aut Qian, Aidong verfasserin aut Li, Ying verfasserin aut Sun, Wuwen verfasserin aut Shan, Xiaofeng verfasserin aut Liu, Ning verfasserin aut Zhang, Lei verfasserin (orcid)0000-0002-1869-5096 aut Enthalten in Microbial pathogenesis Amsterdam [u.a.] : Elsevier, 1986 162 Online-Ressource (DE-627)267840144 (DE-600)1471158-8 (DE-576)121465195 1096-1208 nnns volume:162 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.43 Medizinische Mikrobiologie VZ 44.75 Infektionskrankheiten parasitäre Krankheiten Medizin VZ AR 162
allfieldsSound	10.1016/j.micpath.2021.105374 doi (DE-627)ELV009873600 (ELSEVIER)S0882-4010(21)00648-3 DE-627 ger DE-627 rda eng 610 VZ 44.43 bkl 44.75 bkl Yu, Huabo verfasserin aut A phage cocktail in controlling phage resistance development in multidrug resistant 2021 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). Phage therapy in vivo showed that the survival rate in the mixture phage treatment group was significantly higher than that in single phage treatment group. Bacteriophage Phage cocktail therapy Phage resistant mutants Zhang, Liang verfasserin aut Feng, Chao verfasserin aut Chi, Teng verfasserin aut Qi, Yanling verfasserin aut Abbas Raza, Sayed Haidar verfasserin aut Gao, Na verfasserin aut Jia, Kaixiang verfasserin aut Zhang, Yang verfasserin aut Fan, Ruining verfasserin aut Cai, Ruopeng verfasserin aut Qian, Aidong verfasserin aut Li, Ying verfasserin aut Sun, Wuwen verfasserin aut Shan, Xiaofeng verfasserin aut Liu, Ning verfasserin aut Zhang, Lei verfasserin (orcid)0000-0002-1869-5096 aut Enthalten in Microbial pathogenesis Amsterdam [u.a.] : Elsevier, 1986 162 Online-Ressource (DE-627)267840144 (DE-600)1471158-8 (DE-576)121465195 1096-1208 nnns volume:162 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_224 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2038 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2065 GBV_ILN_2068 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2113 GBV_ILN_2118 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2147 GBV_ILN_2148 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2336 GBV_ILN_2507 GBV_ILN_2522 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4242 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4313 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4335 GBV_ILN_4338 GBV_ILN_4393 44.43 Medizinische Mikrobiologie VZ 44.75 Infektionskrankheiten parasitäre Krankheiten Medizin VZ AR 162
language	English
source	Enthalten in Microbial pathogenesis 162 volume:162
sourceStr	Enthalten in Microbial pathogenesis 162 volume:162
format_phy_str_mv	Article
bklname	Medizinische Mikrobiologie Infektionskrankheiten parasitäre Krankheiten
institution	findex.gbv.de
topic_facet	Bacteriophage Phage cocktail therapy Phage resistant mutants
dewey-raw	610
isfreeaccess_bool	false
container_title	Microbial pathogenesis
authorswithroles_txt_mv	Yu, Huabo @@aut@@ Zhang, Liang @@aut@@ Feng, Chao @@aut@@ Chi, Teng @@aut@@ Qi, Yanling @@aut@@ Abbas Raza, Sayed Haidar @@aut@@ Gao, Na @@aut@@ Jia, Kaixiang @@aut@@ Zhang, Yang @@aut@@ Fan, Ruining @@aut@@ Cai, Ruopeng @@aut@@ Qian, Aidong @@aut@@ Li, Ying @@aut@@ Sun, Wuwen @@aut@@ Shan, Xiaofeng @@aut@@ Liu, Ning @@aut@@ Zhang, Lei @@aut@@
publishDateDaySort_date	2021-01-01T00:00:00Z
hierarchy_top_id	267840144
dewey-sort	3610
id	ELV009873600
language_de	englisch
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author	Yu, Huabo
spellingShingle	Yu, Huabo ddc 610 bkl 44.43 bkl 44.75 misc Bacteriophage misc Phage cocktail therapy misc Phage resistant mutants A phage cocktail in controlling phage resistance development in multidrug resistant
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topic_title	610 VZ 44.43 bkl 44.75 bkl A phage cocktail in controlling phage resistance development in multidrug resistant Bacteriophage Phage cocktail therapy Phage resistant mutants
topic	ddc 610 bkl 44.43 bkl 44.75 misc Bacteriophage misc Phage cocktail therapy misc Phage resistant mutants
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title	A phage cocktail in controlling phage resistance development in multidrug resistant
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title_full	A phage cocktail in controlling phage resistance development in multidrug resistant
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author_browse	Yu, Huabo Zhang, Liang Feng, Chao Chi, Teng Qi, Yanling Abbas Raza, Sayed Haidar Gao, Na Jia, Kaixiang Zhang, Yang Fan, Ruining Cai, Ruopeng Qian, Aidong Li, Ying Sun, Wuwen Shan, Xiaofeng Liu, Ning Zhang, Lei
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title_sort	a phage cocktail in controlling phage resistance development in multidrug resistant
title_auth	A phage cocktail in controlling phage resistance development in multidrug resistant
abstract	Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). Phage therapy in vivo showed that the survival rate in the mixture phage treatment group was significantly higher than that in single phage treatment group.
abstractGer	Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). Phage therapy in vivo showed that the survival rate in the mixture phage treatment group was significantly higher than that in single phage treatment group.
abstract_unstemmed	Aeromonas hydrophila (A. hydrophila) is an opportunistic pathogen of fish-human-livestock, which poses a threat to the development of aquaculture. Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). Phage therapy in vivo showed that the survival rate in the mixture phage treatment group was significantly higher than that in single phage treatment group.
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title_short	A phage cocktail in controlling phage resistance development in multidrug resistant
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author2	Zhang, Liang Feng, Chao Chi, Teng Qi, Yanling Abbas Raza, Sayed Haidar Gao, Na Jia, Kaixiang Zhang, Yang Fan, Ruining Cai, Ruopeng Qian, Aidong Li, Ying Sun, Wuwen Shan, Xiaofeng Liu, Ning Zhang, Lei
author2Str	Zhang, Liang Feng, Chao Chi, Teng Qi, Yanling Abbas Raza, Sayed Haidar Gao, Na Jia, Kaixiang Zhang, Yang Fan, Ruining Cai, Ruopeng Qian, Aidong Li, Ying Sun, Wuwen Shan, Xiaofeng Liu, Ning Zhang, Lei
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up_date	2024-07-07T00:38:58.747Z
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Lytic phage has long been considered as an effective bactericidal agent. However, the rapid development of phage resistance seriously hinders the continuous application of lytic phages. In our study, a new bacteriophage vB_ AhaP_PZL-Ah8 was isolated from sewage and its characteristics and genome were investigated. Phage vB_ AhaP_PZL-Ah8 has been classified as the member of the Podoviridae family, which exhibited the latent period was about 30 min. As revealed from the genomic sequence analysis, vB_ AhaP_PZL-Ah8 covered a double-stranded genome of 40,855 bp (exhibiting 51.89% G + C content), with encoding 52 predicted open reading frames (ORFs). The results suggested that the combination of vB_ AhaP_PZL-Ah8 and another A. hydrophila phage vB_ AhaP_PZL-Ah1 could improve the therapeutic efficacy both in vitro and in vivo. The resistance mutation frequency of A. hydrophila cells infected with the mixture phage (vB_ AhaP_PZL-Ah8+ vB_ AhaP_PZL-Ah1) was significantly lower than cells treated with single phage (P <0.01). 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A phage cocktail in controlling phage resistance development in multidrug resistant

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