A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies

African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this...
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Gespeichert in:

Autor*in:	Wang, Junrong [verfasserIn] Bai, Juan [verfasserIn] Zhang, Lujie [verfasserIn] Xia, Tingting [verfasserIn] Yang, Xing [verfasserIn] Zhang, Keshan [verfasserIn] Gao, Yanni [verfasserIn] Jiang, Ping [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	ASFV PC129R protein Monoclonal antibodies B cell epitope

Übergeordnetes Werk:	Enthalten in: Veterinary microbiology - Amsterdam [u.a.] : Elsevier Science, 1976, 282
Übergeordnetes Werk:	volume:282

DOI / URN:	10.1016/j.vetmic.2023.109744

Katalog-ID:	ELV010027769

Internformat


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245	1	0	\|a A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies
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520			\|a African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this study, 5–6-week-old female BALB/c mice were immunized with rpC129R protein expressed by a prokaryotic system. And three hybridomas, 1B1, 1B4 and 4H4, steadily secreted anti-pC129R monoclonal antibodies were screened by an indirect enzyme linked immunosorbent assay (ELISA). Among them, 1B4 and 4H4 had IgG2a isotype with Kappa light chain, while 1B1 had IgG1 isotype with Kappa light chain. Western blot and indirect immunofluorescence assay showed that three monoclonal antibodies (mAbs) specifically reacted with ASFV. Epitope mapping was performed with truncated polypeptides. And a new B cell epitope, 18KHYVLIPK25 was identified by the mAbs, which was highly conserved in most genotypes of ASFV. These findings not only provide a monoclonal antibody tool for further study of the function of C129R, but also lay the foundation for serological diagnosis and vaccine development.
650		4	\|a ASFV
650		4	\|a PC129R protein
650		4	\|a Monoclonal antibodies
650		4	\|a B cell epitope
700	1		\|a Bai, Juan \|e verfasserin \|4 aut
700	1		\|a Zhang, Lujie \|e verfasserin \|4 aut
700	1		\|a Xia, Tingting \|e verfasserin \|4 aut
700	1		\|a Yang, Xing \|e verfasserin \|4 aut
700	1		\|a Zhang, Keshan \|e verfasserin \|4 aut
700	1		\|a Gao, Yanni \|e verfasserin \|4 aut
700	1		\|a Jiang, Ping \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Veterinary microbiology \|d Amsterdam [u.a.] : Elsevier Science, 1976 \|g 282 \|h Online-Ressource \|w (DE-627)306593963 \|w (DE-600)1498996-7 \|w (DE-576)120883503 \|x 1873-2542 \|7 nnns
773	1	8	\|g volume:282
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912			\|a GBV_ILN_62
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
912			\|a GBV_ILN_70
912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_100
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_151
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_252
912			\|a GBV_ILN_370
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912			\|a GBV_ILN_2011
912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
912			\|a GBV_ILN_2122
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912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
936	b	k	\|a 46.50 \|j Veterinärmedizinische Mikrobiologie \|q VZ
951			\|a AR
952			\|d 282

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author_variant	j w jw j b jb l z lz t x tx x y xy k z kz y g yg p j pj
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publishDate	2023
allfields	10.1016/j.vetmic.2023.109744 doi (DE-627)ELV010027769 (ELSEVIER)S0378-1135(23)00096-2 DE-627 ger DE-627 rda eng 630 VZ 22 ssgn 46.50 bkl Wang, Junrong verfasserin aut A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this study, 5–6-week-old female BALB/c mice were immunized with rpC129R protein expressed by a prokaryotic system. And three hybridomas, 1B1, 1B4 and 4H4, steadily secreted anti-pC129R monoclonal antibodies were screened by an indirect enzyme linked immunosorbent assay (ELISA). Among them, 1B4 and 4H4 had IgG2a isotype with Kappa light chain, while 1B1 had IgG1 isotype with Kappa light chain. Western blot and indirect immunofluorescence assay showed that three monoclonal antibodies (mAbs) specifically reacted with ASFV. Epitope mapping was performed with truncated polypeptides. And a new B cell epitope, 18KHYVLIPK25 was identified by the mAbs, which was highly conserved in most genotypes of ASFV. These findings not only provide a monoclonal antibody tool for further study of the function of C129R, but also lay the foundation for serological diagnosis and vaccine development. ASFV PC129R protein Monoclonal antibodies B cell epitope Bai, Juan verfasserin aut Zhang, Lujie verfasserin aut Xia, Tingting verfasserin aut Yang, Xing verfasserin aut Zhang, Keshan verfasserin aut Gao, Yanni verfasserin aut Jiang, Ping verfasserin aut Enthalten in Veterinary microbiology Amsterdam [u.a.] : Elsevier Science, 1976 282 Online-Ressource (DE-627)306593963 (DE-600)1498996-7 (DE-576)120883503 1873-2542 nnns volume:282 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 46.50 Veterinärmedizinische Mikrobiologie VZ AR 282
spelling	10.1016/j.vetmic.2023.109744 doi (DE-627)ELV010027769 (ELSEVIER)S0378-1135(23)00096-2 DE-627 ger DE-627 rda eng 630 VZ 22 ssgn 46.50 bkl Wang, Junrong verfasserin aut A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this study, 5–6-week-old female BALB/c mice were immunized with rpC129R protein expressed by a prokaryotic system. And three hybridomas, 1B1, 1B4 and 4H4, steadily secreted anti-pC129R monoclonal antibodies were screened by an indirect enzyme linked immunosorbent assay (ELISA). Among them, 1B4 and 4H4 had IgG2a isotype with Kappa light chain, while 1B1 had IgG1 isotype with Kappa light chain. Western blot and indirect immunofluorescence assay showed that three monoclonal antibodies (mAbs) specifically reacted with ASFV. Epitope mapping was performed with truncated polypeptides. And a new B cell epitope, 18KHYVLIPK25 was identified by the mAbs, which was highly conserved in most genotypes of ASFV. These findings not only provide a monoclonal antibody tool for further study of the function of C129R, but also lay the foundation for serological diagnosis and vaccine development. ASFV PC129R protein Monoclonal antibodies B cell epitope Bai, Juan verfasserin aut Zhang, Lujie verfasserin aut Xia, Tingting verfasserin aut Yang, Xing verfasserin aut Zhang, Keshan verfasserin aut Gao, Yanni verfasserin aut Jiang, Ping verfasserin aut Enthalten in Veterinary microbiology Amsterdam [u.a.] : Elsevier Science, 1976 282 Online-Ressource (DE-627)306593963 (DE-600)1498996-7 (DE-576)120883503 1873-2542 nnns volume:282 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 46.50 Veterinärmedizinische Mikrobiologie VZ AR 282
allfields_unstemmed	10.1016/j.vetmic.2023.109744 doi (DE-627)ELV010027769 (ELSEVIER)S0378-1135(23)00096-2 DE-627 ger DE-627 rda eng 630 VZ 22 ssgn 46.50 bkl Wang, Junrong verfasserin aut A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this study, 5–6-week-old female BALB/c mice were immunized with rpC129R protein expressed by a prokaryotic system. And three hybridomas, 1B1, 1B4 and 4H4, steadily secreted anti-pC129R monoclonal antibodies were screened by an indirect enzyme linked immunosorbent assay (ELISA). Among them, 1B4 and 4H4 had IgG2a isotype with Kappa light chain, while 1B1 had IgG1 isotype with Kappa light chain. Western blot and indirect immunofluorescence assay showed that three monoclonal antibodies (mAbs) specifically reacted with ASFV. Epitope mapping was performed with truncated polypeptides. And a new B cell epitope, 18KHYVLIPK25 was identified by the mAbs, which was highly conserved in most genotypes of ASFV. These findings not only provide a monoclonal antibody tool for further study of the function of C129R, but also lay the foundation for serological diagnosis and vaccine development. ASFV PC129R protein Monoclonal antibodies B cell epitope Bai, Juan verfasserin aut Zhang, Lujie verfasserin aut Xia, Tingting verfasserin aut Yang, Xing verfasserin aut Zhang, Keshan verfasserin aut Gao, Yanni verfasserin aut Jiang, Ping verfasserin aut Enthalten in Veterinary microbiology Amsterdam [u.a.] : Elsevier Science, 1976 282 Online-Ressource (DE-627)306593963 (DE-600)1498996-7 (DE-576)120883503 1873-2542 nnns volume:282 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 46.50 Veterinärmedizinische Mikrobiologie VZ AR 282
allfieldsGer	10.1016/j.vetmic.2023.109744 doi (DE-627)ELV010027769 (ELSEVIER)S0378-1135(23)00096-2 DE-627 ger DE-627 rda eng 630 VZ 22 ssgn 46.50 bkl Wang, Junrong verfasserin aut A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this study, 5–6-week-old female BALB/c mice were immunized with rpC129R protein expressed by a prokaryotic system. And three hybridomas, 1B1, 1B4 and 4H4, steadily secreted anti-pC129R monoclonal antibodies were screened by an indirect enzyme linked immunosorbent assay (ELISA). Among them, 1B4 and 4H4 had IgG2a isotype with Kappa light chain, while 1B1 had IgG1 isotype with Kappa light chain. Western blot and indirect immunofluorescence assay showed that three monoclonal antibodies (mAbs) specifically reacted with ASFV. Epitope mapping was performed with truncated polypeptides. And a new B cell epitope, 18KHYVLIPK25 was identified by the mAbs, which was highly conserved in most genotypes of ASFV. These findings not only provide a monoclonal antibody tool for further study of the function of C129R, but also lay the foundation for serological diagnosis and vaccine development. ASFV PC129R protein Monoclonal antibodies B cell epitope Bai, Juan verfasserin aut Zhang, Lujie verfasserin aut Xia, Tingting verfasserin aut Yang, Xing verfasserin aut Zhang, Keshan verfasserin aut Gao, Yanni verfasserin aut Jiang, Ping verfasserin aut Enthalten in Veterinary microbiology Amsterdam [u.a.] : Elsevier Science, 1976 282 Online-Ressource (DE-627)306593963 (DE-600)1498996-7 (DE-576)120883503 1873-2542 nnns volume:282 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 46.50 Veterinärmedizinische Mikrobiologie VZ AR 282
allfieldsSound	10.1016/j.vetmic.2023.109744 doi (DE-627)ELV010027769 (ELSEVIER)S0378-1135(23)00096-2 DE-627 ger DE-627 rda eng 630 VZ 22 ssgn 46.50 bkl Wang, Junrong verfasserin aut A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this study, 5–6-week-old female BALB/c mice were immunized with rpC129R protein expressed by a prokaryotic system. And three hybridomas, 1B1, 1B4 and 4H4, steadily secreted anti-pC129R monoclonal antibodies were screened by an indirect enzyme linked immunosorbent assay (ELISA). Among them, 1B4 and 4H4 had IgG2a isotype with Kappa light chain, while 1B1 had IgG1 isotype with Kappa light chain. Western blot and indirect immunofluorescence assay showed that three monoclonal antibodies (mAbs) specifically reacted with ASFV. Epitope mapping was performed with truncated polypeptides. And a new B cell epitope, 18KHYVLIPK25 was identified by the mAbs, which was highly conserved in most genotypes of ASFV. These findings not only provide a monoclonal antibody tool for further study of the function of C129R, but also lay the foundation for serological diagnosis and vaccine development. ASFV PC129R protein Monoclonal antibodies B cell epitope Bai, Juan verfasserin aut Zhang, Lujie verfasserin aut Xia, Tingting verfasserin aut Yang, Xing verfasserin aut Zhang, Keshan verfasserin aut Gao, Yanni verfasserin aut Jiang, Ping verfasserin aut Enthalten in Veterinary microbiology Amsterdam [u.a.] : Elsevier Science, 1976 282 Online-Ressource (DE-627)306593963 (DE-600)1498996-7 (DE-576)120883503 1873-2542 nnns volume:282 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_252 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 46.50 Veterinärmedizinische Mikrobiologie VZ AR 282
language	English
source	Enthalten in Veterinary microbiology 282 volume:282
sourceStr	Enthalten in Veterinary microbiology 282 volume:282
format_phy_str_mv	Article
bklname	Veterinärmedizinische Mikrobiologie
institution	findex.gbv.de
topic_facet	ASFV PC129R protein Monoclonal antibodies B cell epitope
dewey-raw	630
isfreeaccess_bool	false
container_title	Veterinary microbiology
authorswithroles_txt_mv	Wang, Junrong @@aut@@ Bai, Juan @@aut@@ Zhang, Lujie @@aut@@ Xia, Tingting @@aut@@ Yang, Xing @@aut@@ Zhang, Keshan @@aut@@ Gao, Yanni @@aut@@ Jiang, Ping @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	306593963
dewey-sort	3630
id	ELV010027769
language_de	englisch
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author	Wang, Junrong
spellingShingle	Wang, Junrong ddc 630 ssgn 22 bkl 46.50 misc ASFV misc PC129R protein misc Monoclonal antibodies misc B cell epitope A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies
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topic_title	630 VZ 22 ssgn 46.50 bkl A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies ASFV PC129R protein Monoclonal antibodies B cell epitope
topic	ddc 630 ssgn 22 bkl 46.50 misc ASFV misc PC129R protein misc Monoclonal antibodies misc B cell epitope
topic_unstemmed	ddc 630 ssgn 22 bkl 46.50 misc ASFV misc PC129R protein misc Monoclonal antibodies misc B cell epitope
topic_browse	ddc 630 ssgn 22 bkl 46.50 misc ASFV misc PC129R protein misc Monoclonal antibodies misc B cell epitope
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title	A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies
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title_full	A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies
author_sort	Wang, Junrong
journal	Veterinary microbiology
journalStr	Veterinary microbiology
lang_code	eng
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author_browse	Wang, Junrong Bai, Juan Zhang, Lujie Xia, Tingting Yang, Xing Zhang, Keshan Gao, Yanni Jiang, Ping
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class	630 VZ 22 ssgn 46.50 bkl
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author-letter	Wang, Junrong
doi_str_mv	10.1016/j.vetmic.2023.109744
dewey-full	630
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title_sort	a new b cell epitope of pc129r protein of african swine fever virus identified by monoclonal antibodies
title_auth	A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies
abstract	African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this study, 5–6-week-old female BALB/c mice were immunized with rpC129R protein expressed by a prokaryotic system. And three hybridomas, 1B1, 1B4 and 4H4, steadily secreted anti-pC129R monoclonal antibodies were screened by an indirect enzyme linked immunosorbent assay (ELISA). Among them, 1B4 and 4H4 had IgG2a isotype with Kappa light chain, while 1B1 had IgG1 isotype with Kappa light chain. Western blot and indirect immunofluorescence assay showed that three monoclonal antibodies (mAbs) specifically reacted with ASFV. Epitope mapping was performed with truncated polypeptides. And a new B cell epitope, 18KHYVLIPK25 was identified by the mAbs, which was highly conserved in most genotypes of ASFV. These findings not only provide a monoclonal antibody tool for further study of the function of C129R, but also lay the foundation for serological diagnosis and vaccine development.
abstractGer	African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this study, 5–6-week-old female BALB/c mice were immunized with rpC129R protein expressed by a prokaryotic system. And three hybridomas, 1B1, 1B4 and 4H4, steadily secreted anti-pC129R monoclonal antibodies were screened by an indirect enzyme linked immunosorbent assay (ELISA). Among them, 1B4 and 4H4 had IgG2a isotype with Kappa light chain, while 1B1 had IgG1 isotype with Kappa light chain. Western blot and indirect immunofluorescence assay showed that three monoclonal antibodies (mAbs) specifically reacted with ASFV. Epitope mapping was performed with truncated polypeptides. And a new B cell epitope, 18KHYVLIPK25 was identified by the mAbs, which was highly conserved in most genotypes of ASFV. These findings not only provide a monoclonal antibody tool for further study of the function of C129R, but also lay the foundation for serological diagnosis and vaccine development.
abstract_unstemmed	African swine fever virus (ASFV) is a most important pathogen which causes huge damage in swine production in the world. pC129R protein is one of the most abundant ASFV proteins in infected Vero cells and WSL-HP cells, which consequently could be a target for ASF detection and surveillance. In this study, 5–6-week-old female BALB/c mice were immunized with rpC129R protein expressed by a prokaryotic system. And three hybridomas, 1B1, 1B4 and 4H4, steadily secreted anti-pC129R monoclonal antibodies were screened by an indirect enzyme linked immunosorbent assay (ELISA). Among them, 1B4 and 4H4 had IgG2a isotype with Kappa light chain, while 1B1 had IgG1 isotype with Kappa light chain. Western blot and indirect immunofluorescence assay showed that three monoclonal antibodies (mAbs) specifically reacted with ASFV. Epitope mapping was performed with truncated polypeptides. And a new B cell epitope, 18KHYVLIPK25 was identified by the mAbs, which was highly conserved in most genotypes of ASFV. These findings not only provide a monoclonal antibody tool for further study of the function of C129R, but also lay the foundation for serological diagnosis and vaccine development.
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title_short	A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies
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author2	Bai, Juan Zhang, Lujie Xia, Tingting Yang, Xing Zhang, Keshan Gao, Yanni Jiang, Ping
author2Str	Bai, Juan Zhang, Lujie Xia, Tingting Yang, Xing Zhang, Keshan Gao, Yanni Jiang, Ping
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doi_str	10.1016/j.vetmic.2023.109744
up_date	2024-07-06T16:34:17.553Z
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A new B cell epitope of pC129R protein of African swine fever virus identified by monoclonal antibodies

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