Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching

Fertilized cropland soils are a major source of anthropogenic N2O emissions in terrestrial ecosystems. However, the main N2O production pathways and microbes underlying N2O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used 15N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N2O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N2O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N2O production. Autotrophic nitrification accounted for 47–63% and 76–100% of total N2O emissions in unfertilized (F0) and N-fertilized soils, respectively. AOB accounted for 62% and 91–98% of total N2O production in F0 and N-fertilized soils, respectively. N2O production was significantly lower in soils following long-term film mulching (F225) compared with the bare land soil (N225), and this was due to the reduced abundance and activity of AOB and N2O derived from autotrophic nitrification. Autotrophic nitrification-derived N2O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F225 treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F380), and the manure addition treatment (F225+M) compared with F0, respectively, indicating that autotrophic nitrification was the main driver of increases in N2O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N2O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N2O mitigation strategies. Ausführliche Beschreibung

Gespeichert in:

Autor*in:	Gao, Na [verfasserIn] Yang, Bin [verfasserIn] Song, Qilong [verfasserIn] Li, Xin [verfasserIn] Chen, WenQian [verfasserIn] Shen, Yufang [verfasserIn] Yue, Shanchao [verfasserIn] Li, Shiqing [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Mulching N Ammonia-oxidizing bacteria Gene abundance Autotrophic nitrification

Übergeordnetes Werk:	Enthalten in: Geoderma - Amsterdam [u.a.] : Elsevier Science, 1967, 435
Übergeordnetes Werk:	volume:435

DOI / URN:	10.1016/j.geoderma.2023.116523

Katalog-ID:	ELV010039422

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allfields	10.1016/j.geoderma.2023.116523 doi (DE-627)ELV010039422 (ELSEVIER)S0016-7061(23)00200-8 DE-627 ger DE-627 rda eng 550 910 VZ 38.60 bkl Gao, Na verfasserin aut Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fertilized cropland soils are a major source of anthropogenic N2O emissions in terrestrial ecosystems. However, the main N2O production pathways and microbes underlying N2O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used 15N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N2O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N2O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N2O production. Autotrophic nitrification accounted for 47–63% and 76–100% of total N2O emissions in unfertilized (F0) and N-fertilized soils, respectively. AOB accounted for 62% and 91–98% of total N2O production in F0 and N-fertilized soils, respectively. N2O production was significantly lower in soils following long-term film mulching (F225) compared with the bare land soil (N225), and this was due to the reduced abundance and activity of AOB and N2O derived from autotrophic nitrification. Autotrophic nitrification-derived N2O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F225 treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F380), and the manure addition treatment (F225+M) compared with F0, respectively, indicating that autotrophic nitrification was the main driver of increases in N2O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N2O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N2O mitigation strategies. Mulching N Ammonia-oxidizing bacteria Gene abundance Autotrophic nitrification Yang, Bin verfasserin (orcid)0000-0001-8081-2292 aut Song, Qilong verfasserin aut Li, Xin verfasserin (orcid)0000-0002-9035-9775 aut Chen, WenQian verfasserin aut Shen, Yufang verfasserin aut Yue, Shanchao verfasserin (orcid)0000-0001-8724-2974 aut Li, Shiqing verfasserin aut Enthalten in Geoderma Amsterdam [u.a.] : Elsevier Science, 1967 435 Online-Ressource (DE-627)320414493 (DE-600)2001729-7 (DE-576)099603853 1872-6259 nnns volume:435 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2014 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 38.60 Bodenkunde: Allgemeines Geowissenschaften VZ AR 435
spelling	10.1016/j.geoderma.2023.116523 doi (DE-627)ELV010039422 (ELSEVIER)S0016-7061(23)00200-8 DE-627 ger DE-627 rda eng 550 910 VZ 38.60 bkl Gao, Na verfasserin aut Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fertilized cropland soils are a major source of anthropogenic N2O emissions in terrestrial ecosystems. However, the main N2O production pathways and microbes underlying N2O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used 15N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N2O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N2O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N2O production. Autotrophic nitrification accounted for 47–63% and 76–100% of total N2O emissions in unfertilized (F0) and N-fertilized soils, respectively. AOB accounted for 62% and 91–98% of total N2O production in F0 and N-fertilized soils, respectively. N2O production was significantly lower in soils following long-term film mulching (F225) compared with the bare land soil (N225), and this was due to the reduced abundance and activity of AOB and N2O derived from autotrophic nitrification. Autotrophic nitrification-derived N2O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F225 treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F380), and the manure addition treatment (F225+M) compared with F0, respectively, indicating that autotrophic nitrification was the main driver of increases in N2O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N2O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N2O mitigation strategies. Mulching N Ammonia-oxidizing bacteria Gene abundance Autotrophic nitrification Yang, Bin verfasserin (orcid)0000-0001-8081-2292 aut Song, Qilong verfasserin aut Li, Xin verfasserin (orcid)0000-0002-9035-9775 aut Chen, WenQian verfasserin aut Shen, Yufang verfasserin aut Yue, Shanchao verfasserin (orcid)0000-0001-8724-2974 aut Li, Shiqing verfasserin aut Enthalten in Geoderma Amsterdam [u.a.] : Elsevier Science, 1967 435 Online-Ressource (DE-627)320414493 (DE-600)2001729-7 (DE-576)099603853 1872-6259 nnns volume:435 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2014 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 38.60 Bodenkunde: Allgemeines Geowissenschaften VZ AR 435
allfields_unstemmed	10.1016/j.geoderma.2023.116523 doi (DE-627)ELV010039422 (ELSEVIER)S0016-7061(23)00200-8 DE-627 ger DE-627 rda eng 550 910 VZ 38.60 bkl Gao, Na verfasserin aut Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fertilized cropland soils are a major source of anthropogenic N2O emissions in terrestrial ecosystems. However, the main N2O production pathways and microbes underlying N2O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used 15N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N2O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N2O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N2O production. Autotrophic nitrification accounted for 47–63% and 76–100% of total N2O emissions in unfertilized (F0) and N-fertilized soils, respectively. AOB accounted for 62% and 91–98% of total N2O production in F0 and N-fertilized soils, respectively. N2O production was significantly lower in soils following long-term film mulching (F225) compared with the bare land soil (N225), and this was due to the reduced abundance and activity of AOB and N2O derived from autotrophic nitrification. Autotrophic nitrification-derived N2O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F225 treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F380), and the manure addition treatment (F225+M) compared with F0, respectively, indicating that autotrophic nitrification was the main driver of increases in N2O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N2O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N2O mitigation strategies. Mulching N Ammonia-oxidizing bacteria Gene abundance Autotrophic nitrification Yang, Bin verfasserin (orcid)0000-0001-8081-2292 aut Song, Qilong verfasserin aut Li, Xin verfasserin (orcid)0000-0002-9035-9775 aut Chen, WenQian verfasserin aut Shen, Yufang verfasserin aut Yue, Shanchao verfasserin (orcid)0000-0001-8724-2974 aut Li, Shiqing verfasserin aut Enthalten in Geoderma Amsterdam [u.a.] : Elsevier Science, 1967 435 Online-Ressource (DE-627)320414493 (DE-600)2001729-7 (DE-576)099603853 1872-6259 nnns volume:435 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2014 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 38.60 Bodenkunde: Allgemeines Geowissenschaften VZ AR 435
allfieldsGer	10.1016/j.geoderma.2023.116523 doi (DE-627)ELV010039422 (ELSEVIER)S0016-7061(23)00200-8 DE-627 ger DE-627 rda eng 550 910 VZ 38.60 bkl Gao, Na verfasserin aut Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fertilized cropland soils are a major source of anthropogenic N2O emissions in terrestrial ecosystems. However, the main N2O production pathways and microbes underlying N2O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used 15N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N2O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N2O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N2O production. Autotrophic nitrification accounted for 47–63% and 76–100% of total N2O emissions in unfertilized (F0) and N-fertilized soils, respectively. AOB accounted for 62% and 91–98% of total N2O production in F0 and N-fertilized soils, respectively. N2O production was significantly lower in soils following long-term film mulching (F225) compared with the bare land soil (N225), and this was due to the reduced abundance and activity of AOB and N2O derived from autotrophic nitrification. Autotrophic nitrification-derived N2O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F225 treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F380), and the manure addition treatment (F225+M) compared with F0, respectively, indicating that autotrophic nitrification was the main driver of increases in N2O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N2O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N2O mitigation strategies. Mulching N Ammonia-oxidizing bacteria Gene abundance Autotrophic nitrification Yang, Bin verfasserin (orcid)0000-0001-8081-2292 aut Song, Qilong verfasserin aut Li, Xin verfasserin (orcid)0000-0002-9035-9775 aut Chen, WenQian verfasserin aut Shen, Yufang verfasserin aut Yue, Shanchao verfasserin (orcid)0000-0001-8724-2974 aut Li, Shiqing verfasserin aut Enthalten in Geoderma Amsterdam [u.a.] : Elsevier Science, 1967 435 Online-Ressource (DE-627)320414493 (DE-600)2001729-7 (DE-576)099603853 1872-6259 nnns volume:435 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2014 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 38.60 Bodenkunde: Allgemeines Geowissenschaften VZ AR 435
allfieldsSound	10.1016/j.geoderma.2023.116523 doi (DE-627)ELV010039422 (ELSEVIER)S0016-7061(23)00200-8 DE-627 ger DE-627 rda eng 550 910 VZ 38.60 bkl Gao, Na verfasserin aut Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Fertilized cropland soils are a major source of anthropogenic N2O emissions in terrestrial ecosystems. However, the main N2O production pathways and microbes underlying N2O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used 15N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N2O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N2O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N2O production. Autotrophic nitrification accounted for 47–63% and 76–100% of total N2O emissions in unfertilized (F0) and N-fertilized soils, respectively. AOB accounted for 62% and 91–98% of total N2O production in F0 and N-fertilized soils, respectively. N2O production was significantly lower in soils following long-term film mulching (F225) compared with the bare land soil (N225), and this was due to the reduced abundance and activity of AOB and N2O derived from autotrophic nitrification. Autotrophic nitrification-derived N2O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F225 treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F380), and the manure addition treatment (F225+M) compared with F0, respectively, indicating that autotrophic nitrification was the main driver of increases in N2O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N2O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N2O mitigation strategies. Mulching N Ammonia-oxidizing bacteria Gene abundance Autotrophic nitrification Yang, Bin verfasserin (orcid)0000-0001-8081-2292 aut Song, Qilong verfasserin aut Li, Xin verfasserin (orcid)0000-0002-9035-9775 aut Chen, WenQian verfasserin aut Shen, Yufang verfasserin aut Yue, Shanchao verfasserin (orcid)0000-0001-8724-2974 aut Li, Shiqing verfasserin aut Enthalten in Geoderma Amsterdam [u.a.] : Elsevier Science, 1967 435 Online-Ressource (DE-627)320414493 (DE-600)2001729-7 (DE-576)099603853 1872-6259 nnns volume:435 GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2014 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 38.60 Bodenkunde: Allgemeines Geowissenschaften VZ AR 435
language	English
source	Enthalten in Geoderma 435 volume:435
sourceStr	Enthalten in Geoderma 435 volume:435
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bklname	Bodenkunde: Allgemeines
institution	findex.gbv.de
topic_facet	Mulching N Ammonia-oxidizing bacteria Gene abundance Autotrophic nitrification
dewey-raw	550
isfreeaccess_bool	false
container_title	Geoderma
authorswithroles_txt_mv	Gao, Na @@aut@@ Yang, Bin @@aut@@ Song, Qilong @@aut@@ Li, Xin @@aut@@ Chen, WenQian @@aut@@ Shen, Yufang @@aut@@ Yue, Shanchao @@aut@@ Li, Shiqing @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
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author	Gao, Na
spellingShingle	Gao, Na ddc 550 bkl 38.60 misc Mulching misc N misc Ammonia-oxidizing bacteria misc Gene abundance misc Autotrophic nitrification Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching
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topic_title	550 910 VZ 38.60 bkl Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching Mulching N Ammonia-oxidizing bacteria Gene abundance Autotrophic nitrification
topic	ddc 550 bkl 38.60 misc Mulching misc N misc Ammonia-oxidizing bacteria misc Gene abundance misc Autotrophic nitrification
topic_unstemmed	ddc 550 bkl 38.60 misc Mulching misc N misc Ammonia-oxidizing bacteria misc Gene abundance misc Autotrophic nitrification
topic_browse	ddc 550 bkl 38.60 misc Mulching misc N misc Ammonia-oxidizing bacteria misc Gene abundance misc Autotrophic nitrification
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title	Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching
ctrlnum	(DE-627)ELV010039422 (ELSEVIER)S0016-7061(23)00200-8
title_full	Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching
author_sort	Gao, Na
journal	Geoderma
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author_browse	Gao, Na Yang, Bin Song, Qilong Li, Xin Chen, WenQian Shen, Yufang Yue, Shanchao Li, Shiqing
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title_sort	ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching
title_auth	Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching
abstract	Fertilized cropland soils are a major source of anthropogenic N2O emissions in terrestrial ecosystems. However, the main N2O production pathways and microbes underlying N2O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used 15N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N2O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N2O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N2O production. Autotrophic nitrification accounted for 47–63% and 76–100% of total N2O emissions in unfertilized (F0) and N-fertilized soils, respectively. AOB accounted for 62% and 91–98% of total N2O production in F0 and N-fertilized soils, respectively. N2O production was significantly lower in soils following long-term film mulching (F225) compared with the bare land soil (N225), and this was due to the reduced abundance and activity of AOB and N2O derived from autotrophic nitrification. Autotrophic nitrification-derived N2O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F225 treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F380), and the manure addition treatment (F225+M) compared with F0, respectively, indicating that autotrophic nitrification was the main driver of increases in N2O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N2O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N2O mitigation strategies.
abstractGer	Fertilized cropland soils are a major source of anthropogenic N2O emissions in terrestrial ecosystems. However, the main N2O production pathways and microbes underlying N2O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used 15N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N2O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N2O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N2O production. Autotrophic nitrification accounted for 47–63% and 76–100% of total N2O emissions in unfertilized (F0) and N-fertilized soils, respectively. AOB accounted for 62% and 91–98% of total N2O production in F0 and N-fertilized soils, respectively. N2O production was significantly lower in soils following long-term film mulching (F225) compared with the bare land soil (N225), and this was due to the reduced abundance and activity of AOB and N2O derived from autotrophic nitrification. Autotrophic nitrification-derived N2O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F225 treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F380), and the manure addition treatment (F225+M) compared with F0, respectively, indicating that autotrophic nitrification was the main driver of increases in N2O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N2O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N2O mitigation strategies.
abstract_unstemmed	Fertilized cropland soils are a major source of anthropogenic N2O emissions in terrestrial ecosystems. However, the main N2O production pathways and microbes underlying N2O production in calcareous cropland soil under long-term plastic film mulching and nitrogen (N) management have not yet been clarified. Here, we used 15N tracing combined with quantitative polymerase chain reaction to evaluate the relative contributions of different N2O production pathways (i.e., autotrophic nitrification, heterotrophic nitrification, and denitrification) to N2O production in a calcareous maize field soil; we also used specific inhibitors (acetylene and 1-octyne) to determine the functional importance of ammonia-oxidizing bacteria (AOB) and archaea (AOA) to N2O production. Autotrophic nitrification accounted for 47–63% and 76–100% of total N2O emissions in unfertilized (F0) and N-fertilized soils, respectively. AOB accounted for 62% and 91–98% of total N2O production in F0 and N-fertilized soils, respectively. N2O production was significantly lower in soils following long-term film mulching (F225) compared with the bare land soil (N225), and this was due to the reduced abundance and activity of AOB and N2O derived from autotrophic nitrification. Autotrophic nitrification-derived N2O emissions were 2.2-fold, 3.6-fold, and 6.3-fold higher in the F225 treatment (appropriate N level), the treatment in which the level of N was the same as that applied by local farmers (F380), and the manure addition treatment (F225+M) compared with F0, respectively, indicating that autotrophic nitrification was the main driver of increases in N2O emissions. These findings provide the first direct evidence showing that AOB-driven autotrophic nitrification is the dominant N2O production pathway in the plastic film mulched calcareous soils under different agricultural management practices. Our results also show that autotrophic nitrification is depressed by long-term mulching and promoted by N fertilization. These findings have implications for the development of targeted N2O mitigation strategies.
collection_details	GBV_USEFLAG_U SYSFLAG_U GBV_ELV SSG-OPC-GGO GBV_ILN_11 GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_370 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2014 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700
title_short	Ammonia-oxidizing bacteria-driven autotrophic nitrification dominated nitrous oxide production in calcareous soil under long term plastic film mulching
remote_bool	true
author2	Yang, Bin Song, Qilong Li, Xin Chen, WenQian Shen, Yufang Yue, Shanchao Li, Shiqing
author2Str	Yang, Bin Song, Qilong Li, Xin Chen, WenQian Shen, Yufang Yue, Shanchao Li, Shiqing
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doi_str	10.1016/j.geoderma.2023.116523
up_date	2024-07-06T16:36:43.041Z
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