A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer (

Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer...
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Gespeichert in:

Autor*in:	Liu, Guoqiang [verfasserIn] Luo, Jianxing [verfasserIn] Xu, Weiliang [verfasserIn] Li, Chundong [verfasserIn] Guo, Liang [verfasserIn]

Format:	E-Artikel
Sprache:	Englisch

Erschienen:	2023

Schlagwörter:	Triplex real-time PCR Sika deer Red deer Antlers Meat Authentication

Übergeordnetes Werk:	Enthalten in: Journal of food composition and analysis - Orlando, Fla. : Academic Press, 1987, 121
Übergeordnetes Werk:	volume:121

DOI / URN:	10.1016/j.jfca.2023.105390

Katalog-ID:	ELV010315128

Internformat


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100	1		\|a Liu, Guoqiang \|e verfasserin \|4 aut
245	1	0	\|a A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer (
264		1	\|c 2023
336			\|a nicht spezifiziert \|b zzz \|2 rdacontent
337			\|a Computermedien \|b c \|2 rdamedia
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520			\|a Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer and red deer in deer products. The triplex real-time PCR approach showed high specificity for 10 animal species, and the method simultaneously amplified an endogenous control to eliminate false-negative results. The absolute limits of detection for raw meat, meat product, and antlers were 0.00005–0.000025 ng, 0.00005–0.000025 ng, and 0.0001–0.000025 ng, respectively, for sika deer and 0.00005–0.000025 ng, 0.0005–0.00025 ng, and 0.0025–0.00025 ng, respectively, for red deer. This approach could determine 0.1% (w/w) adulteration. In conclusion, the established triplex real-time PCR assay displayed high specificity and sensitivity, and applicability and can be used for authentic animal products from sika deer and red deer.Data Availability: Data will be made available on request.
650		4	\|a Triplex real-time PCR
650		4	\|a Sika deer
650		4	\|a Red deer
650		4	\|a Antlers
650		4	\|a Meat
650		4	\|a Authentication
700	1		\|a Luo, Jianxing \|e verfasserin \|4 aut
700	1		\|a Xu, Weiliang \|e verfasserin \|4 aut
700	1		\|a Li, Chundong \|e verfasserin \|4 aut
700	1		\|a Guo, Liang \|e verfasserin \|4 aut
773	0	8	\|i Enthalten in \|t Journal of food composition and analysis \|d Orlando, Fla. : Academic Press, 1987 \|g 121 \|h Online-Ressource \|w (DE-627)267328400 \|w (DE-600)1469801-8 \|w (DE-576)259483710 \|x 0889-1575 \|7 nnns
773	1	8	\|g volume:121
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912			\|a GBV_ILN_32
912			\|a GBV_ILN_40
912			\|a GBV_ILN_60
912			\|a GBV_ILN_62
912			\|a GBV_ILN_65
912			\|a GBV_ILN_69
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912			\|a GBV_ILN_73
912			\|a GBV_ILN_74
912			\|a GBV_ILN_90
912			\|a GBV_ILN_95
912			\|a GBV_ILN_100
912			\|a GBV_ILN_101
912			\|a GBV_ILN_105
912			\|a GBV_ILN_110
912			\|a GBV_ILN_150
912			\|a GBV_ILN_151
912			\|a GBV_ILN_187
912			\|a GBV_ILN_213
912			\|a GBV_ILN_224
912			\|a GBV_ILN_230
912			\|a GBV_ILN_370
912			\|a GBV_ILN_602
912			\|a GBV_ILN_702
912			\|a GBV_ILN_2001
912			\|a GBV_ILN_2003
912			\|a GBV_ILN_2004
912			\|a GBV_ILN_2005
912			\|a GBV_ILN_2007
912			\|a GBV_ILN_2008
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912			\|a GBV_ILN_2010
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912			\|a GBV_ILN_2014
912			\|a GBV_ILN_2015
912			\|a GBV_ILN_2020
912			\|a GBV_ILN_2021
912			\|a GBV_ILN_2025
912			\|a GBV_ILN_2026
912			\|a GBV_ILN_2027
912			\|a GBV_ILN_2034
912			\|a GBV_ILN_2044
912			\|a GBV_ILN_2048
912			\|a GBV_ILN_2049
912			\|a GBV_ILN_2050
912			\|a GBV_ILN_2055
912			\|a GBV_ILN_2056
912			\|a GBV_ILN_2059
912			\|a GBV_ILN_2061
912			\|a GBV_ILN_2064
912			\|a GBV_ILN_2088
912			\|a GBV_ILN_2106
912			\|a GBV_ILN_2110
912			\|a GBV_ILN_2111
912			\|a GBV_ILN_2112
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912			\|a GBV_ILN_2336
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912			\|a GBV_ILN_4035
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912			\|a GBV_ILN_4112
912			\|a GBV_ILN_4125
912			\|a GBV_ILN_4242
912			\|a GBV_ILN_4249
912			\|a GBV_ILN_4251
912			\|a GBV_ILN_4305
912			\|a GBV_ILN_4306
912			\|a GBV_ILN_4307
912			\|a GBV_ILN_4313
912			\|a GBV_ILN_4322
912			\|a GBV_ILN_4323
912			\|a GBV_ILN_4324
912			\|a GBV_ILN_4325
912			\|a GBV_ILN_4326
912			\|a GBV_ILN_4333
912			\|a GBV_ILN_4334
912			\|a GBV_ILN_4338
912			\|a GBV_ILN_4393
912			\|a GBV_ILN_4700
936	b	k	\|a 58.34 \|j Lebensmitteltechnologie \|q VZ
951			\|a AR
952			\|d 121

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publishDate	2023
allfields	10.1016/j.jfca.2023.105390 doi (DE-627)ELV010315128 (ELSEVIER)S0889-1575(23)00264-8 DE-627 ger DE-627 rda eng 630 640 540 660 VZ 58.34 bkl Liu, Guoqiang verfasserin aut A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer ( 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer and red deer in deer products. The triplex real-time PCR approach showed high specificity for 10 animal species, and the method simultaneously amplified an endogenous control to eliminate false-negative results. The absolute limits of detection for raw meat, meat product, and antlers were 0.00005–0.000025 ng, 0.00005–0.000025 ng, and 0.0001–0.000025 ng, respectively, for sika deer and 0.00005–0.000025 ng, 0.0005–0.00025 ng, and 0.0025–0.00025 ng, respectively, for red deer. This approach could determine 0.1% (w/w) adulteration. In conclusion, the established triplex real-time PCR assay displayed high specificity and sensitivity, and applicability and can be used for authentic animal products from sika deer and red deer.Data Availability: Data will be made available on request. Triplex real-time PCR Sika deer Red deer Antlers Meat Authentication Luo, Jianxing verfasserin aut Xu, Weiliang verfasserin aut Li, Chundong verfasserin aut Guo, Liang verfasserin aut Enthalten in Journal of food composition and analysis Orlando, Fla. : Academic Press, 1987 121 Online-Ressource (DE-627)267328400 (DE-600)1469801-8 (DE-576)259483710 0889-1575 nnns volume:121 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 121
spelling	10.1016/j.jfca.2023.105390 doi (DE-627)ELV010315128 (ELSEVIER)S0889-1575(23)00264-8 DE-627 ger DE-627 rda eng 630 640 540 660 VZ 58.34 bkl Liu, Guoqiang verfasserin aut A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer ( 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer and red deer in deer products. The triplex real-time PCR approach showed high specificity for 10 animal species, and the method simultaneously amplified an endogenous control to eliminate false-negative results. The absolute limits of detection for raw meat, meat product, and antlers were 0.00005–0.000025 ng, 0.00005–0.000025 ng, and 0.0001–0.000025 ng, respectively, for sika deer and 0.00005–0.000025 ng, 0.0005–0.00025 ng, and 0.0025–0.00025 ng, respectively, for red deer. This approach could determine 0.1% (w/w) adulteration. In conclusion, the established triplex real-time PCR assay displayed high specificity and sensitivity, and applicability and can be used for authentic animal products from sika deer and red deer.Data Availability: Data will be made available on request. Triplex real-time PCR Sika deer Red deer Antlers Meat Authentication Luo, Jianxing verfasserin aut Xu, Weiliang verfasserin aut Li, Chundong verfasserin aut Guo, Liang verfasserin aut Enthalten in Journal of food composition and analysis Orlando, Fla. : Academic Press, 1987 121 Online-Ressource (DE-627)267328400 (DE-600)1469801-8 (DE-576)259483710 0889-1575 nnns volume:121 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 121
allfields_unstemmed	10.1016/j.jfca.2023.105390 doi (DE-627)ELV010315128 (ELSEVIER)S0889-1575(23)00264-8 DE-627 ger DE-627 rda eng 630 640 540 660 VZ 58.34 bkl Liu, Guoqiang verfasserin aut A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer ( 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer and red deer in deer products. The triplex real-time PCR approach showed high specificity for 10 animal species, and the method simultaneously amplified an endogenous control to eliminate false-negative results. The absolute limits of detection for raw meat, meat product, and antlers were 0.00005–0.000025 ng, 0.00005–0.000025 ng, and 0.0001–0.000025 ng, respectively, for sika deer and 0.00005–0.000025 ng, 0.0005–0.00025 ng, and 0.0025–0.00025 ng, respectively, for red deer. This approach could determine 0.1% (w/w) adulteration. In conclusion, the established triplex real-time PCR assay displayed high specificity and sensitivity, and applicability and can be used for authentic animal products from sika deer and red deer.Data Availability: Data will be made available on request. Triplex real-time PCR Sika deer Red deer Antlers Meat Authentication Luo, Jianxing verfasserin aut Xu, Weiliang verfasserin aut Li, Chundong verfasserin aut Guo, Liang verfasserin aut Enthalten in Journal of food composition and analysis Orlando, Fla. : Academic Press, 1987 121 Online-Ressource (DE-627)267328400 (DE-600)1469801-8 (DE-576)259483710 0889-1575 nnns volume:121 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 121
allfieldsGer	10.1016/j.jfca.2023.105390 doi (DE-627)ELV010315128 (ELSEVIER)S0889-1575(23)00264-8 DE-627 ger DE-627 rda eng 630 640 540 660 VZ 58.34 bkl Liu, Guoqiang verfasserin aut A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer ( 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer and red deer in deer products. The triplex real-time PCR approach showed high specificity for 10 animal species, and the method simultaneously amplified an endogenous control to eliminate false-negative results. The absolute limits of detection for raw meat, meat product, and antlers were 0.00005–0.000025 ng, 0.00005–0.000025 ng, and 0.0001–0.000025 ng, respectively, for sika deer and 0.00005–0.000025 ng, 0.0005–0.00025 ng, and 0.0025–0.00025 ng, respectively, for red deer. This approach could determine 0.1% (w/w) adulteration. In conclusion, the established triplex real-time PCR assay displayed high specificity and sensitivity, and applicability and can be used for authentic animal products from sika deer and red deer.Data Availability: Data will be made available on request. Triplex real-time PCR Sika deer Red deer Antlers Meat Authentication Luo, Jianxing verfasserin aut Xu, Weiliang verfasserin aut Li, Chundong verfasserin aut Guo, Liang verfasserin aut Enthalten in Journal of food composition and analysis Orlando, Fla. : Academic Press, 1987 121 Online-Ressource (DE-627)267328400 (DE-600)1469801-8 (DE-576)259483710 0889-1575 nnns volume:121 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 121
allfieldsSound	10.1016/j.jfca.2023.105390 doi (DE-627)ELV010315128 (ELSEVIER)S0889-1575(23)00264-8 DE-627 ger DE-627 rda eng 630 640 540 660 VZ 58.34 bkl Liu, Guoqiang verfasserin aut A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer ( 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer and red deer in deer products. The triplex real-time PCR approach showed high specificity for 10 animal species, and the method simultaneously amplified an endogenous control to eliminate false-negative results. The absolute limits of detection for raw meat, meat product, and antlers were 0.00005–0.000025 ng, 0.00005–0.000025 ng, and 0.0001–0.000025 ng, respectively, for sika deer and 0.00005–0.000025 ng, 0.0005–0.00025 ng, and 0.0025–0.00025 ng, respectively, for red deer. This approach could determine 0.1% (w/w) adulteration. In conclusion, the established triplex real-time PCR assay displayed high specificity and sensitivity, and applicability and can be used for authentic animal products from sika deer and red deer.Data Availability: Data will be made available on request. Triplex real-time PCR Sika deer Red deer Antlers Meat Authentication Luo, Jianxing verfasserin aut Xu, Weiliang verfasserin aut Li, Chundong verfasserin aut Guo, Liang verfasserin aut Enthalten in Journal of food composition and analysis Orlando, Fla. : Academic Press, 1987 121 Online-Ressource (DE-627)267328400 (DE-600)1469801-8 (DE-576)259483710 0889-1575 nnns volume:121 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_95 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_150 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 58.34 Lebensmitteltechnologie VZ AR 121
language	English
source	Enthalten in Journal of food composition and analysis 121 volume:121
sourceStr	Enthalten in Journal of food composition and analysis 121 volume:121
format_phy_str_mv	Article
bklname	Lebensmitteltechnologie
institution	findex.gbv.de
topic_facet	Triplex real-time PCR Sika deer Red deer Antlers Meat Authentication
dewey-raw	630
isfreeaccess_bool	false
container_title	Journal of food composition and analysis
authorswithroles_txt_mv	Liu, Guoqiang @@aut@@ Luo, Jianxing @@aut@@ Xu, Weiliang @@aut@@ Li, Chundong @@aut@@ Guo, Liang @@aut@@
publishDateDaySort_date	2023-01-01T00:00:00Z
hierarchy_top_id	267328400
dewey-sort	3630
id	ELV010315128
language_de	englisch
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author	Liu, Guoqiang
spellingShingle	Liu, Guoqiang ddc 630 bkl 58.34 misc Triplex real-time PCR misc Sika deer misc Red deer misc Antlers misc Meat misc Authentication A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer (
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topic_title	630 640 540 660 VZ 58.34 bkl A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer ( Triplex real-time PCR Sika deer Red deer Antlers Meat Authentication
topic	ddc 630 bkl 58.34 misc Triplex real-time PCR misc Sika deer misc Red deer misc Antlers misc Meat misc Authentication
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title	A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer (
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title_full	A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer (
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title_sort	a novel triplex real-time pcr method for the simultaneous authentication of meats and antlers from sika deer (
title_auth	A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer (
abstract	Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer and red deer in deer products. The triplex real-time PCR approach showed high specificity for 10 animal species, and the method simultaneously amplified an endogenous control to eliminate false-negative results. The absolute limits of detection for raw meat, meat product, and antlers were 0.00005–0.000025 ng, 0.00005–0.000025 ng, and 0.0001–0.000025 ng, respectively, for sika deer and 0.00005–0.000025 ng, 0.0005–0.00025 ng, and 0.0025–0.00025 ng, respectively, for red deer. This approach could determine 0.1% (w/w) adulteration. In conclusion, the established triplex real-time PCR assay displayed high specificity and sensitivity, and applicability and can be used for authentic animal products from sika deer and red deer.Data Availability: Data will be made available on request.
abstractGer	Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer and red deer in deer products. The triplex real-time PCR approach showed high specificity for 10 animal species, and the method simultaneously amplified an endogenous control to eliminate false-negative results. The absolute limits of detection for raw meat, meat product, and antlers were 0.00005–0.000025 ng, 0.00005–0.000025 ng, and 0.0001–0.000025 ng, respectively, for sika deer and 0.00005–0.000025 ng, 0.0005–0.00025 ng, and 0.0025–0.00025 ng, respectively, for red deer. This approach could determine 0.1% (w/w) adulteration. In conclusion, the established triplex real-time PCR assay displayed high specificity and sensitivity, and applicability and can be used for authentic animal products from sika deer and red deer.Data Availability: Data will be made available on request.
abstract_unstemmed	Deer products have been used as a traditional Chinese medical and edible food for over 2000 years. Due to the high price, the meats and antlers are often adulterated with low-value materials. The present study developed a triplex real-time PCR assay to simultaneously identify the DNA from sika deer and red deer in deer products. The triplex real-time PCR approach showed high specificity for 10 animal species, and the method simultaneously amplified an endogenous control to eliminate false-negative results. The absolute limits of detection for raw meat, meat product, and antlers were 0.00005–0.000025 ng, 0.00005–0.000025 ng, and 0.0001–0.000025 ng, respectively, for sika deer and 0.00005–0.000025 ng, 0.0005–0.00025 ng, and 0.0025–0.00025 ng, respectively, for red deer. This approach could determine 0.1% (w/w) adulteration. In conclusion, the established triplex real-time PCR assay displayed high specificity and sensitivity, and applicability and can be used for authentic animal products from sika deer and red deer.Data Availability: Data will be made available on request.
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title_short	A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer (
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up_date	2024-07-06T17:33:34.126Z
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A novel triplex real-time PCR method for the simultaneous authentication of meats and antlers from sika deer (

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