Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase
As a new generation of green solvents, deep eutectic solvents (DESs) are considered a promising alternative to current harsh organic solvents and find application in many chemical processing methods such as extraction and synthesis. DESs, normally formed by two or more components via various hydroge...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Romano, Angela [verfasserIn] Varriale, Simona [verfasserIn] Pezzella, Cinzia [verfasserIn] Totaro, Grazia [verfasserIn] Andanson, Jean-Michel [verfasserIn] Verney, Vincent [verfasserIn] Sisti, Laura [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: New biotechnology - New York, NY [u.a.] : Elsevier, 2008, 76, Seite 118-126 |
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| Übergeordnetes Werk: |
volume:76 ; pages:118-126 |
| DOI / URN: |
10.1016/j.nbt.2023.05.006 |
|---|
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ELV010470662 |
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| 520 | |a As a new generation of green solvents, deep eutectic solvents (DESs) are considered a promising alternative to current harsh organic solvents and find application in many chemical processing methods such as extraction and synthesis. DESs, normally formed by two or more components via various hydrogen bond interactions, offer high potential as medium for biocatalysis reactions where they can improve efficiency by enhancing substrate solubility and the activity and stability of the enzymes. In the current study, the stabilization of Humicola insolens cutinase (HiC) in natural deep eutectic solvents (NADESs) was assessed. The best hydrogen bond donor among sorbitol, xylitol, erythritol, glycerol and ethylene glycol, and the best acceptor among betaine, choline chloride, choline acetate, choline dihydrogen citrate and tetramethylammonium chloride, were selected, evaluating binding energies and molecular orientations through molecular docking simulations, and finally used to prepare NADES aqueous solutions. The effects of component ratio and NADES concentration on HiC thermostability at 90 °C were also investigated. The choline dihydrogen citrate:xylitol, in a 1:1 ratio with a 20 wt% concentration, was selected as the best combination in stabilizing HiC, increasing its half-life three-fold. | ||
| 650 | 4 | |a NADES | |
| 650 | 4 | |a Enzyme stability | |
| 650 | 4 | |a Molecular docking | |
| 650 | 4 | |a Choline dihydrogencitrate | |
| 650 | 4 | |a Xylitol | |
| 700 | 1 | |a Varriale, Simona |e verfasserin |0 (orcid)0000-0003-3054-3375 |4 aut | |
| 700 | 1 | |a Pezzella, Cinzia |e verfasserin |0 (orcid)0000-0001-5694-472X |4 aut | |
| 700 | 1 | |a Totaro, Grazia |e verfasserin |0 (orcid)0000-0002-7388-8894 |4 aut | |
| 700 | 1 | |a Andanson, Jean-Michel |e verfasserin |0 (orcid)0000-0002-1610-9313 |4 aut | |
| 700 | 1 | |a Verney, Vincent |e verfasserin |0 (orcid)0000-0002-3900-9366 |4 aut | |
| 700 | 1 | |a Sisti, Laura |e verfasserin |4 aut | |
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10.1016/j.nbt.2023.05.006 doi (DE-627)ELV010470662 (ELSEVIER)S1871-6784(23)00027-4 DE-627 ger DE-627 rda eng 570 540 VZ 42.13 bkl 35.71 bkl Romano, Angela verfasserin (orcid)0000-0002-9479-7844 aut Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a new generation of green solvents, deep eutectic solvents (DESs) are considered a promising alternative to current harsh organic solvents and find application in many chemical processing methods such as extraction and synthesis. DESs, normally formed by two or more components via various hydrogen bond interactions, offer high potential as medium for biocatalysis reactions where they can improve efficiency by enhancing substrate solubility and the activity and stability of the enzymes. In the current study, the stabilization of Humicola insolens cutinase (HiC) in natural deep eutectic solvents (NADESs) was assessed. The best hydrogen bond donor among sorbitol, xylitol, erythritol, glycerol and ethylene glycol, and the best acceptor among betaine, choline chloride, choline acetate, choline dihydrogen citrate and tetramethylammonium chloride, were selected, evaluating binding energies and molecular orientations through molecular docking simulations, and finally used to prepare NADES aqueous solutions. The effects of component ratio and NADES concentration on HiC thermostability at 90 °C were also investigated. The choline dihydrogen citrate:xylitol, in a 1:1 ratio with a 20 wt% concentration, was selected as the best combination in stabilizing HiC, increasing its half-life three-fold. NADES Enzyme stability Molecular docking Choline dihydrogencitrate Xylitol Varriale, Simona verfasserin (orcid)0000-0003-3054-3375 aut Pezzella, Cinzia verfasserin (orcid)0000-0001-5694-472X aut Totaro, Grazia verfasserin (orcid)0000-0002-7388-8894 aut Andanson, Jean-Michel verfasserin (orcid)0000-0002-1610-9313 aut Verney, Vincent verfasserin (orcid)0000-0002-3900-9366 aut Sisti, Laura verfasserin aut Enthalten in New biotechnology New York, NY [u.a.] : Elsevier, 2008 76, Seite 118-126 Online-Ressource (DE-627)55568895X (DE-600)2400836-9 (DE-576)281507724 1876-4347 nnns volume:76 pages:118-126 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 42.13 Molekularbiologie VZ 35.71 Biochemische Methoden VZ AR 76 118-126 |
| spelling |
10.1016/j.nbt.2023.05.006 doi (DE-627)ELV010470662 (ELSEVIER)S1871-6784(23)00027-4 DE-627 ger DE-627 rda eng 570 540 VZ 42.13 bkl 35.71 bkl Romano, Angela verfasserin (orcid)0000-0002-9479-7844 aut Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a new generation of green solvents, deep eutectic solvents (DESs) are considered a promising alternative to current harsh organic solvents and find application in many chemical processing methods such as extraction and synthesis. DESs, normally formed by two or more components via various hydrogen bond interactions, offer high potential as medium for biocatalysis reactions where they can improve efficiency by enhancing substrate solubility and the activity and stability of the enzymes. In the current study, the stabilization of Humicola insolens cutinase (HiC) in natural deep eutectic solvents (NADESs) was assessed. The best hydrogen bond donor among sorbitol, xylitol, erythritol, glycerol and ethylene glycol, and the best acceptor among betaine, choline chloride, choline acetate, choline dihydrogen citrate and tetramethylammonium chloride, were selected, evaluating binding energies and molecular orientations through molecular docking simulations, and finally used to prepare NADES aqueous solutions. The effects of component ratio and NADES concentration on HiC thermostability at 90 °C were also investigated. The choline dihydrogen citrate:xylitol, in a 1:1 ratio with a 20 wt% concentration, was selected as the best combination in stabilizing HiC, increasing its half-life three-fold. NADES Enzyme stability Molecular docking Choline dihydrogencitrate Xylitol Varriale, Simona verfasserin (orcid)0000-0003-3054-3375 aut Pezzella, Cinzia verfasserin (orcid)0000-0001-5694-472X aut Totaro, Grazia verfasserin (orcid)0000-0002-7388-8894 aut Andanson, Jean-Michel verfasserin (orcid)0000-0002-1610-9313 aut Verney, Vincent verfasserin (orcid)0000-0002-3900-9366 aut Sisti, Laura verfasserin aut Enthalten in New biotechnology New York, NY [u.a.] : Elsevier, 2008 76, Seite 118-126 Online-Ressource (DE-627)55568895X (DE-600)2400836-9 (DE-576)281507724 1876-4347 nnns volume:76 pages:118-126 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 42.13 Molekularbiologie VZ 35.71 Biochemische Methoden VZ AR 76 118-126 |
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10.1016/j.nbt.2023.05.006 doi (DE-627)ELV010470662 (ELSEVIER)S1871-6784(23)00027-4 DE-627 ger DE-627 rda eng 570 540 VZ 42.13 bkl 35.71 bkl Romano, Angela verfasserin (orcid)0000-0002-9479-7844 aut Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a new generation of green solvents, deep eutectic solvents (DESs) are considered a promising alternative to current harsh organic solvents and find application in many chemical processing methods such as extraction and synthesis. DESs, normally formed by two or more components via various hydrogen bond interactions, offer high potential as medium for biocatalysis reactions where they can improve efficiency by enhancing substrate solubility and the activity and stability of the enzymes. In the current study, the stabilization of Humicola insolens cutinase (HiC) in natural deep eutectic solvents (NADESs) was assessed. The best hydrogen bond donor among sorbitol, xylitol, erythritol, glycerol and ethylene glycol, and the best acceptor among betaine, choline chloride, choline acetate, choline dihydrogen citrate and tetramethylammonium chloride, were selected, evaluating binding energies and molecular orientations through molecular docking simulations, and finally used to prepare NADES aqueous solutions. The effects of component ratio and NADES concentration on HiC thermostability at 90 °C were also investigated. The choline dihydrogen citrate:xylitol, in a 1:1 ratio with a 20 wt% concentration, was selected as the best combination in stabilizing HiC, increasing its half-life three-fold. NADES Enzyme stability Molecular docking Choline dihydrogencitrate Xylitol Varriale, Simona verfasserin (orcid)0000-0003-3054-3375 aut Pezzella, Cinzia verfasserin (orcid)0000-0001-5694-472X aut Totaro, Grazia verfasserin (orcid)0000-0002-7388-8894 aut Andanson, Jean-Michel verfasserin (orcid)0000-0002-1610-9313 aut Verney, Vincent verfasserin (orcid)0000-0002-3900-9366 aut Sisti, Laura verfasserin aut Enthalten in New biotechnology New York, NY [u.a.] : Elsevier, 2008 76, Seite 118-126 Online-Ressource (DE-627)55568895X (DE-600)2400836-9 (DE-576)281507724 1876-4347 nnns volume:76 pages:118-126 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 42.13 Molekularbiologie VZ 35.71 Biochemische Methoden VZ AR 76 118-126 |
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10.1016/j.nbt.2023.05.006 doi (DE-627)ELV010470662 (ELSEVIER)S1871-6784(23)00027-4 DE-627 ger DE-627 rda eng 570 540 VZ 42.13 bkl 35.71 bkl Romano, Angela verfasserin (orcid)0000-0002-9479-7844 aut Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier As a new generation of green solvents, deep eutectic solvents (DESs) are considered a promising alternative to current harsh organic solvents and find application in many chemical processing methods such as extraction and synthesis. DESs, normally formed by two or more components via various hydrogen bond interactions, offer high potential as medium for biocatalysis reactions where they can improve efficiency by enhancing substrate solubility and the activity and stability of the enzymes. In the current study, the stabilization of Humicola insolens cutinase (HiC) in natural deep eutectic solvents (NADESs) was assessed. The best hydrogen bond donor among sorbitol, xylitol, erythritol, glycerol and ethylene glycol, and the best acceptor among betaine, choline chloride, choline acetate, choline dihydrogen citrate and tetramethylammonium chloride, were selected, evaluating binding energies and molecular orientations through molecular docking simulations, and finally used to prepare NADES aqueous solutions. The effects of component ratio and NADES concentration on HiC thermostability at 90 °C were also investigated. The choline dihydrogen citrate:xylitol, in a 1:1 ratio with a 20 wt% concentration, was selected as the best combination in stabilizing HiC, increasing its half-life three-fold. NADES Enzyme stability Molecular docking Choline dihydrogencitrate Xylitol Varriale, Simona verfasserin (orcid)0000-0003-3054-3375 aut Pezzella, Cinzia verfasserin (orcid)0000-0001-5694-472X aut Totaro, Grazia verfasserin (orcid)0000-0002-7388-8894 aut Andanson, Jean-Michel verfasserin (orcid)0000-0002-1610-9313 aut Verney, Vincent verfasserin (orcid)0000-0002-3900-9366 aut Sisti, Laura verfasserin aut Enthalten in New biotechnology New York, NY [u.a.] : Elsevier, 2008 76, Seite 118-126 Online-Ressource (DE-627)55568895X (DE-600)2400836-9 (DE-576)281507724 1876-4347 nnns volume:76 pages:118-126 GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_39 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_63 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_95 GBV_ILN_105 GBV_ILN_110 GBV_ILN_151 GBV_ILN_161 GBV_ILN_170 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_285 GBV_ILN_293 GBV_ILN_602 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2008 GBV_ILN_2014 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2064 GBV_ILN_2106 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2232 GBV_ILN_2336 GBV_ILN_4012 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4126 GBV_ILN_4249 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4338 GBV_ILN_4367 GBV_ILN_4700 42.13 Molekularbiologie VZ 35.71 Biochemische Methoden VZ AR 76 118-126 |
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natural deep eutectic solvents as thermostabilizer for humicola insolens cutinase |
| title_auth |
Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase |
| abstract |
As a new generation of green solvents, deep eutectic solvents (DESs) are considered a promising alternative to current harsh organic solvents and find application in many chemical processing methods such as extraction and synthesis. DESs, normally formed by two or more components via various hydrogen bond interactions, offer high potential as medium for biocatalysis reactions where they can improve efficiency by enhancing substrate solubility and the activity and stability of the enzymes. In the current study, the stabilization of Humicola insolens cutinase (HiC) in natural deep eutectic solvents (NADESs) was assessed. The best hydrogen bond donor among sorbitol, xylitol, erythritol, glycerol and ethylene glycol, and the best acceptor among betaine, choline chloride, choline acetate, choline dihydrogen citrate and tetramethylammonium chloride, were selected, evaluating binding energies and molecular orientations through molecular docking simulations, and finally used to prepare NADES aqueous solutions. The effects of component ratio and NADES concentration on HiC thermostability at 90 °C were also investigated. The choline dihydrogen citrate:xylitol, in a 1:1 ratio with a 20 wt% concentration, was selected as the best combination in stabilizing HiC, increasing its half-life three-fold. |
| abstractGer |
As a new generation of green solvents, deep eutectic solvents (DESs) are considered a promising alternative to current harsh organic solvents and find application in many chemical processing methods such as extraction and synthesis. DESs, normally formed by two or more components via various hydrogen bond interactions, offer high potential as medium for biocatalysis reactions where they can improve efficiency by enhancing substrate solubility and the activity and stability of the enzymes. In the current study, the stabilization of Humicola insolens cutinase (HiC) in natural deep eutectic solvents (NADESs) was assessed. The best hydrogen bond donor among sorbitol, xylitol, erythritol, glycerol and ethylene glycol, and the best acceptor among betaine, choline chloride, choline acetate, choline dihydrogen citrate and tetramethylammonium chloride, were selected, evaluating binding energies and molecular orientations through molecular docking simulations, and finally used to prepare NADES aqueous solutions. The effects of component ratio and NADES concentration on HiC thermostability at 90 °C were also investigated. The choline dihydrogen citrate:xylitol, in a 1:1 ratio with a 20 wt% concentration, was selected as the best combination in stabilizing HiC, increasing its half-life three-fold. |
| abstract_unstemmed |
As a new generation of green solvents, deep eutectic solvents (DESs) are considered a promising alternative to current harsh organic solvents and find application in many chemical processing methods such as extraction and synthesis. DESs, normally formed by two or more components via various hydrogen bond interactions, offer high potential as medium for biocatalysis reactions where they can improve efficiency by enhancing substrate solubility and the activity and stability of the enzymes. In the current study, the stabilization of Humicola insolens cutinase (HiC) in natural deep eutectic solvents (NADESs) was assessed. The best hydrogen bond donor among sorbitol, xylitol, erythritol, glycerol and ethylene glycol, and the best acceptor among betaine, choline chloride, choline acetate, choline dihydrogen citrate and tetramethylammonium chloride, were selected, evaluating binding energies and molecular orientations through molecular docking simulations, and finally used to prepare NADES aqueous solutions. The effects of component ratio and NADES concentration on HiC thermostability at 90 °C were also investigated. The choline dihydrogen citrate:xylitol, in a 1:1 ratio with a 20 wt% concentration, was selected as the best combination in stabilizing HiC, increasing its half-life three-fold. |
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| title_short |
Natural deep eutectic solvents as thermostabilizer for Humicola insolens cutinase |
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Varriale, Simona Pezzella, Cinzia Totaro, Grazia Andanson, Jean-Michel Verney, Vincent Sisti, Laura |
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| doi_str |
10.1016/j.nbt.2023.05.006 |
| up_date |
2024-07-06T18:06:18.773Z |
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