Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in
Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Song, Shipeng [verfasserIn] Liu, Hui [verfasserIn] Miao, Luke [verfasserIn] He, Li [verfasserIn] Xie, Wenzhao [verfasserIn] Lan, Hong [verfasserIn] Yu, Changxiu [verfasserIn] Yan, Wenkai [verfasserIn] Wu, Yufeng [verfasserIn] Wen, Xiao-peng [verfasserIn] Xu, Qiang [verfasserIn] Deng, Xiuxin [verfasserIn] Chen, Chunli [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2023 |
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| Schlagwörter: |
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| Übergeordnetes Werk: |
Enthalten in: Journal of genetics and genomics - Amsterdam [u.a.] : Elsevier, 2007, 50, Seite 410-421 |
|---|---|
| Übergeordnetes Werk: |
volume:50 ; pages:410-421 |
| DOI / URN: |
10.1016/j.jgg.2022.12.003 |
|---|
| Katalog-ID: |
ELV010524843 |
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| 520 | |a Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions, which enables us to visualize significant differences between homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the translocation regions of chromosomes 4 and 9 in C. sinensis “Valencia.” The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. | ||
| 650 | 4 | |a Chromosomal marker | |
| 650 | 4 | |a FISH | |
| 650 | 4 | |a CMA banding | |
| 650 | 4 | |a Genomic heterozygosity | |
| 650 | 4 | |a Chromosomal translocation | |
| 650 | 4 | |a Sweet orange | |
| 700 | 1 | |a Liu, Hui |e verfasserin |0 (orcid)0000-0003-4927-892X |4 aut | |
| 700 | 1 | |a Miao, Luke |e verfasserin |4 aut | |
| 700 | 1 | |a He, Li |e verfasserin |4 aut | |
| 700 | 1 | |a Xie, Wenzhao |e verfasserin |4 aut | |
| 700 | 1 | |a Lan, Hong |e verfasserin |4 aut | |
| 700 | 1 | |a Yu, Changxiu |e verfasserin |4 aut | |
| 700 | 1 | |a Yan, Wenkai |e verfasserin |4 aut | |
| 700 | 1 | |a Wu, Yufeng |e verfasserin |4 aut | |
| 700 | 1 | |a Wen, Xiao-peng |e verfasserin |4 aut | |
| 700 | 1 | |a Xu, Qiang |e verfasserin |4 aut | |
| 700 | 1 | |a Deng, Xiuxin |e verfasserin |4 aut | |
| 700 | 1 | |a Chen, Chunli |e verfasserin |0 (orcid)0000-0002-6464-4580 |4 aut | |
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10.1016/j.jgg.2022.12.003 doi (DE-627)ELV010524843 (ELSEVIER)S1673-8527(22)00283-1 DE-627 ger DE-627 rda eng 570 VZ ASIEN DE-1a fid 12 ssgn BIODIV DE-30 fid Song, Shipeng verfasserin aut Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions, which enables us to visualize significant differences between homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the translocation regions of chromosomes 4 and 9 in C. sinensis “Valencia.” The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. Chromosomal marker FISH CMA banding Genomic heterozygosity Chromosomal translocation Sweet orange Liu, Hui verfasserin (orcid)0000-0003-4927-892X aut Miao, Luke verfasserin aut He, Li verfasserin aut Xie, Wenzhao verfasserin aut Lan, Hong verfasserin aut Yu, Changxiu verfasserin aut Yan, Wenkai verfasserin aut Wu, Yufeng verfasserin aut Wen, Xiao-peng verfasserin aut Xu, Qiang verfasserin aut Deng, Xiuxin verfasserin aut Chen, Chunli verfasserin (orcid)0000-0002-6464-4580 aut Enthalten in Journal of genetics and genomics Amsterdam [u.a.] : Elsevier, 2007 50, Seite 410-421 Online-Ressource (DE-627)534677924 (DE-600)2374568-X (DE-576)271586680 1873-5533 nnns volume:50 pages:410-421 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-ASIEN FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 50 410-421 |
| spelling |
10.1016/j.jgg.2022.12.003 doi (DE-627)ELV010524843 (ELSEVIER)S1673-8527(22)00283-1 DE-627 ger DE-627 rda eng 570 VZ ASIEN DE-1a fid 12 ssgn BIODIV DE-30 fid Song, Shipeng verfasserin aut Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions, which enables us to visualize significant differences between homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the translocation regions of chromosomes 4 and 9 in C. sinensis “Valencia.” The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. Chromosomal marker FISH CMA banding Genomic heterozygosity Chromosomal translocation Sweet orange Liu, Hui verfasserin (orcid)0000-0003-4927-892X aut Miao, Luke verfasserin aut He, Li verfasserin aut Xie, Wenzhao verfasserin aut Lan, Hong verfasserin aut Yu, Changxiu verfasserin aut Yan, Wenkai verfasserin aut Wu, Yufeng verfasserin aut Wen, Xiao-peng verfasserin aut Xu, Qiang verfasserin aut Deng, Xiuxin verfasserin aut Chen, Chunli verfasserin (orcid)0000-0002-6464-4580 aut Enthalten in Journal of genetics and genomics Amsterdam [u.a.] : Elsevier, 2007 50, Seite 410-421 Online-Ressource (DE-627)534677924 (DE-600)2374568-X (DE-576)271586680 1873-5533 nnns volume:50 pages:410-421 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-ASIEN FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 50 410-421 |
| allfields_unstemmed |
10.1016/j.jgg.2022.12.003 doi (DE-627)ELV010524843 (ELSEVIER)S1673-8527(22)00283-1 DE-627 ger DE-627 rda eng 570 VZ ASIEN DE-1a fid 12 ssgn BIODIV DE-30 fid Song, Shipeng verfasserin aut Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions, which enables us to visualize significant differences between homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the translocation regions of chromosomes 4 and 9 in C. sinensis “Valencia.” The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. Chromosomal marker FISH CMA banding Genomic heterozygosity Chromosomal translocation Sweet orange Liu, Hui verfasserin (orcid)0000-0003-4927-892X aut Miao, Luke verfasserin aut He, Li verfasserin aut Xie, Wenzhao verfasserin aut Lan, Hong verfasserin aut Yu, Changxiu verfasserin aut Yan, Wenkai verfasserin aut Wu, Yufeng verfasserin aut Wen, Xiao-peng verfasserin aut Xu, Qiang verfasserin aut Deng, Xiuxin verfasserin aut Chen, Chunli verfasserin (orcid)0000-0002-6464-4580 aut Enthalten in Journal of genetics and genomics Amsterdam [u.a.] : Elsevier, 2007 50, Seite 410-421 Online-Ressource (DE-627)534677924 (DE-600)2374568-X (DE-576)271586680 1873-5533 nnns volume:50 pages:410-421 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-ASIEN FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 50 410-421 |
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10.1016/j.jgg.2022.12.003 doi (DE-627)ELV010524843 (ELSEVIER)S1673-8527(22)00283-1 DE-627 ger DE-627 rda eng 570 VZ ASIEN DE-1a fid 12 ssgn BIODIV DE-30 fid Song, Shipeng verfasserin aut Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions, which enables us to visualize significant differences between homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the translocation regions of chromosomes 4 and 9 in C. sinensis “Valencia.” The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. Chromosomal marker FISH CMA banding Genomic heterozygosity Chromosomal translocation Sweet orange Liu, Hui verfasserin (orcid)0000-0003-4927-892X aut Miao, Luke verfasserin aut He, Li verfasserin aut Xie, Wenzhao verfasserin aut Lan, Hong verfasserin aut Yu, Changxiu verfasserin aut Yan, Wenkai verfasserin aut Wu, Yufeng verfasserin aut Wen, Xiao-peng verfasserin aut Xu, Qiang verfasserin aut Deng, Xiuxin verfasserin aut Chen, Chunli verfasserin (orcid)0000-0002-6464-4580 aut Enthalten in Journal of genetics and genomics Amsterdam [u.a.] : Elsevier, 2007 50, Seite 410-421 Online-Ressource (DE-627)534677924 (DE-600)2374568-X (DE-576)271586680 1873-5533 nnns volume:50 pages:410-421 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-ASIEN FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 50 410-421 |
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10.1016/j.jgg.2022.12.003 doi (DE-627)ELV010524843 (ELSEVIER)S1673-8527(22)00283-1 DE-627 ger DE-627 rda eng 570 VZ ASIEN DE-1a fid 12 ssgn BIODIV DE-30 fid Song, Shipeng verfasserin aut Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in 2023 nicht spezifiziert zzz rdacontent Computermedien c rdamedia Online-Ressource cr rdacarrier Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions, which enables us to visualize significant differences between homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the translocation regions of chromosomes 4 and 9 in C. sinensis “Valencia.” The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. Chromosomal marker FISH CMA banding Genomic heterozygosity Chromosomal translocation Sweet orange Liu, Hui verfasserin (orcid)0000-0003-4927-892X aut Miao, Luke verfasserin aut He, Li verfasserin aut Xie, Wenzhao verfasserin aut Lan, Hong verfasserin aut Yu, Changxiu verfasserin aut Yan, Wenkai verfasserin aut Wu, Yufeng verfasserin aut Wen, Xiao-peng verfasserin aut Xu, Qiang verfasserin aut Deng, Xiuxin verfasserin aut Chen, Chunli verfasserin (orcid)0000-0002-6464-4580 aut Enthalten in Journal of genetics and genomics Amsterdam [u.a.] : Elsevier, 2007 50, Seite 410-421 Online-Ressource (DE-627)534677924 (DE-600)2374568-X (DE-576)271586680 1873-5533 nnns volume:50 pages:410-421 GBV_USEFLAG_U SYSFLAG_U GBV_ELV FID-ASIEN FID-BIODIV SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_23 GBV_ILN_24 GBV_ILN_31 GBV_ILN_32 GBV_ILN_40 GBV_ILN_60 GBV_ILN_62 GBV_ILN_65 GBV_ILN_69 GBV_ILN_70 GBV_ILN_73 GBV_ILN_74 GBV_ILN_90 GBV_ILN_100 GBV_ILN_101 GBV_ILN_105 GBV_ILN_110 GBV_ILN_120 GBV_ILN_121 GBV_ILN_151 GBV_ILN_187 GBV_ILN_213 GBV_ILN_224 GBV_ILN_230 GBV_ILN_370 GBV_ILN_374 GBV_ILN_602 GBV_ILN_702 GBV_ILN_2001 GBV_ILN_2003 GBV_ILN_2004 GBV_ILN_2005 GBV_ILN_2007 GBV_ILN_2008 GBV_ILN_2009 GBV_ILN_2010 GBV_ILN_2011 GBV_ILN_2014 GBV_ILN_2015 GBV_ILN_2020 GBV_ILN_2021 GBV_ILN_2025 GBV_ILN_2026 GBV_ILN_2027 GBV_ILN_2034 GBV_ILN_2044 GBV_ILN_2048 GBV_ILN_2049 GBV_ILN_2050 GBV_ILN_2055 GBV_ILN_2056 GBV_ILN_2059 GBV_ILN_2061 GBV_ILN_2064 GBV_ILN_2068 GBV_ILN_2088 GBV_ILN_2106 GBV_ILN_2110 GBV_ILN_2111 GBV_ILN_2112 GBV_ILN_2122 GBV_ILN_2129 GBV_ILN_2143 GBV_ILN_2152 GBV_ILN_2153 GBV_ILN_2190 GBV_ILN_2232 GBV_ILN_2470 GBV_ILN_2507 GBV_ILN_4035 GBV_ILN_4037 GBV_ILN_4112 GBV_ILN_4125 GBV_ILN_4242 GBV_ILN_4249 GBV_ILN_4251 GBV_ILN_4305 GBV_ILN_4306 GBV_ILN_4307 GBV_ILN_4313 GBV_ILN_4322 GBV_ILN_4323 GBV_ILN_4324 GBV_ILN_4325 GBV_ILN_4326 GBV_ILN_4333 GBV_ILN_4334 GBV_ILN_4338 GBV_ILN_4393 GBV_ILN_4700 AR 50 410-421 |
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Chromosomal marker FISH CMA banding Genomic heterozygosity Chromosomal translocation Sweet orange |
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Song, Shipeng @@aut@@ Liu, Hui @@aut@@ Miao, Luke @@aut@@ He, Li @@aut@@ Xie, Wenzhao @@aut@@ Lan, Hong @@aut@@ Yu, Changxiu @@aut@@ Yan, Wenkai @@aut@@ Wu, Yufeng @@aut@@ Wen, Xiao-peng @@aut@@ Xu, Qiang @@aut@@ Deng, Xiuxin @@aut@@ Chen, Chunli @@aut@@ |
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Song, Shipeng |
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570 VZ ASIEN DE-1a fid 12 ssgn BIODIV DE-30 fid Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in Chromosomal marker FISH CMA banding Genomic heterozygosity Chromosomal translocation Sweet orange |
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ddc 570 fid ASIEN ssgn 12 fid BIODIV misc Chromosomal marker misc FISH misc CMA banding misc Genomic heterozygosity misc Chromosomal translocation misc Sweet orange |
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Song, Shipeng Liu, Hui Miao, Luke He, Li Xie, Wenzhao Lan, Hong Yu, Changxiu Yan, Wenkai Wu, Yufeng Wen, Xiao-peng Xu, Qiang Deng, Xiuxin Chen, Chunli |
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molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in |
| title_auth |
Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in |
| abstract |
Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions, which enables us to visualize significant differences between homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the translocation regions of chromosomes 4 and 9 in C. sinensis “Valencia.” The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. |
| abstractGer |
Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions, which enables us to visualize significant differences between homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the translocation regions of chromosomes 4 and 9 in C. sinensis “Valencia.” The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. |
| abstract_unstemmed |
Citrus sinensis is the most cultivated and economically valuable Citrus species in the world, whose genome has been assembled by three generation sequencings. However, chromosome recognition remains a problem due to the small size of chromosomes, and difficulty in differentiating between pseudo and real chromosomes because of a highly heterozygous genome. Here, we employ fluorescence in situ hybridization (FISH) with 9 chromosome painting probes, 30 oligo pools, and 8 repetitive sequences to visualize 18 chromosomes. Then, we develop an approach to identify each chromosome in one cell through single experiment of oligo-FISH and Chromoycin A3 (CMA) staining. By this approach, we construct a high-resolution molecular cytogenetic map containing the physical positions of CMA banding and 38 sequences of FISH including centromere regions, which enables us to visualize significant differences between homologous chromosomes. Based on the map, we locate several highly repetitive sequences on chromosomes and estimate sizes and copy numbers of each site. In particular, we discover the translocation regions of chromosomes 4 and 9 in C. sinensis “Valencia.” The high-resolution molecular cytogenetic map will help improve understanding of sweet orange genome assembly and also provide a fundamental reference for investigating chromosome evolution and chromosome engineering for genetic improvement in Citrus. |
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Molecular cytogenetic map visualizes the heterozygotic genome and identifies translocation chromosomes in |
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Liu, Hui Miao, Luke He, Li Xie, Wenzhao Lan, Hong Yu, Changxiu Yan, Wenkai Wu, Yufeng Wen, Xiao-peng Xu, Qiang Deng, Xiuxin Chen, Chunli |
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