Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs)
Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for t...
Ausführliche Beschreibung
Autor*in: |
Zhao, Xiubo [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2014transfer abstract |
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Umfang: |
8 |
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Übergeordnetes Werk: |
Enthalten in: Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery - Borgès Da Silva, Virginie ELSEVIER, 2018, Amsterdam [u.a.] |
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Übergeordnetes Werk: |
volume:190 ; year:2014 ; pages:946-953 ; extent:8 |
Links: |
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DOI / URN: |
10.1016/j.snb.2013.09.064 |
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ELV012442984 |
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520 | |a Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. | ||
520 | |a Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. | ||
650 | 7 | |a Cancer diagnostics |2 Elsevier | |
650 | 7 | |a Prostate-specific antigen |2 Elsevier | |
650 | 7 | |a Monoclonal antibody |2 Elsevier | |
650 | 7 | |a Biosensors |2 Elsevier | |
650 | 7 | |a FBAR |2 Elsevier | |
650 | 7 | |a Protein adsorption |2 Elsevier | |
700 | 1 | |a Pan, Fang |4 oth | |
700 | 1 | |a Ashley, Gregory M. |4 oth | |
700 | 1 | |a Garcia-Gancedo, Luis |4 oth | |
700 | 1 | |a Luo, Jikui |4 oth | |
700 | 1 | |a Flewitt, Andrew J. |4 oth | |
700 | 1 | |a Milne, William I. |4 oth | |
700 | 1 | |a Lu, Jian R. |4 oth | |
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10.1016/j.snb.2013.09.064 doi GBVA2014014000024.pica (DE-627)ELV012442984 (ELSEVIER)S0925-4005(13)01105-2 DE-627 ger DE-627 rakwb eng 530 620 530 DE-600 620 DE-600 610 VZ 44.91 bkl Zhao, Xiubo verfasserin aut Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs) 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Cancer diagnostics Elsevier Prostate-specific antigen Elsevier Monoclonal antibody Elsevier Biosensors Elsevier FBAR Elsevier Protein adsorption Elsevier Pan, Fang oth Ashley, Gregory M. oth Garcia-Gancedo, Luis oth Luo, Jikui oth Flewitt, Andrew J. oth Milne, William I. oth Lu, Jian R. oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:190 year:2014 pages:946-953 extent:8 https://doi.org/10.1016/j.snb.2013.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 190 2014 946-953 8 045F 530 |
spelling |
10.1016/j.snb.2013.09.064 doi GBVA2014014000024.pica (DE-627)ELV012442984 (ELSEVIER)S0925-4005(13)01105-2 DE-627 ger DE-627 rakwb eng 530 620 530 DE-600 620 DE-600 610 VZ 44.91 bkl Zhao, Xiubo verfasserin aut Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs) 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Cancer diagnostics Elsevier Prostate-specific antigen Elsevier Monoclonal antibody Elsevier Biosensors Elsevier FBAR Elsevier Protein adsorption Elsevier Pan, Fang oth Ashley, Gregory M. oth Garcia-Gancedo, Luis oth Luo, Jikui oth Flewitt, Andrew J. oth Milne, William I. oth Lu, Jian R. oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:190 year:2014 pages:946-953 extent:8 https://doi.org/10.1016/j.snb.2013.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 190 2014 946-953 8 045F 530 |
allfields_unstemmed |
10.1016/j.snb.2013.09.064 doi GBVA2014014000024.pica (DE-627)ELV012442984 (ELSEVIER)S0925-4005(13)01105-2 DE-627 ger DE-627 rakwb eng 530 620 530 DE-600 620 DE-600 610 VZ 44.91 bkl Zhao, Xiubo verfasserin aut Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs) 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Cancer diagnostics Elsevier Prostate-specific antigen Elsevier Monoclonal antibody Elsevier Biosensors Elsevier FBAR Elsevier Protein adsorption Elsevier Pan, Fang oth Ashley, Gregory M. oth Garcia-Gancedo, Luis oth Luo, Jikui oth Flewitt, Andrew J. oth Milne, William I. oth Lu, Jian R. oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:190 year:2014 pages:946-953 extent:8 https://doi.org/10.1016/j.snb.2013.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 190 2014 946-953 8 045F 530 |
allfieldsGer |
10.1016/j.snb.2013.09.064 doi GBVA2014014000024.pica (DE-627)ELV012442984 (ELSEVIER)S0925-4005(13)01105-2 DE-627 ger DE-627 rakwb eng 530 620 530 DE-600 620 DE-600 610 VZ 44.91 bkl Zhao, Xiubo verfasserin aut Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs) 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Cancer diagnostics Elsevier Prostate-specific antigen Elsevier Monoclonal antibody Elsevier Biosensors Elsevier FBAR Elsevier Protein adsorption Elsevier Pan, Fang oth Ashley, Gregory M. oth Garcia-Gancedo, Luis oth Luo, Jikui oth Flewitt, Andrew J. oth Milne, William I. oth Lu, Jian R. oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:190 year:2014 pages:946-953 extent:8 https://doi.org/10.1016/j.snb.2013.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 190 2014 946-953 8 045F 530 |
allfieldsSound |
10.1016/j.snb.2013.09.064 doi GBVA2014014000024.pica (DE-627)ELV012442984 (ELSEVIER)S0925-4005(13)01105-2 DE-627 ger DE-627 rakwb eng 530 620 530 DE-600 620 DE-600 610 VZ 44.91 bkl Zhao, Xiubo verfasserin aut Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs) 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. Cancer diagnostics Elsevier Prostate-specific antigen Elsevier Monoclonal antibody Elsevier Biosensors Elsevier FBAR Elsevier Protein adsorption Elsevier Pan, Fang oth Ashley, Gregory M. oth Garcia-Gancedo, Luis oth Luo, Jikui oth Flewitt, Andrew J. oth Milne, William I. oth Lu, Jian R. oth Enthalten in Elsevier Science Borgès Da Silva, Virginie ELSEVIER Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery 2018 Amsterdam [u.a.] (DE-627)ELV001079875 volume:190 year:2014 pages:946-953 extent:8 https://doi.org/10.1016/j.snb.2013.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.91 Psychiatrie Psychopathologie VZ AR 190 2014 946-953 8 045F 530 |
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Association between binge eating disorder and psychiatric comorbidity profiles in patients with obesity seeking bariatric surgery |
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label-free detection of human prostate-specific antigen (hpsa) using film bulk acoustic resonators (fbars) |
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Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs) |
abstract |
Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. |
abstractGer |
Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. |
abstract_unstemmed |
Label-free detection of cancer biomarkers using low cost biosensors has promising applications in clinical diagnostics. In this work, ZnO-based thin film bulk acoustic wave resonators (FBARs) with resonant frequency of ∼1.5GHz and mass sensitivity of 0.015mg/m2 (1.5ng/cm2) have been fabricated for their deployment as biosensors. Mouse monoclonal antibody, anti-human prostate-specific antigen (Anti-hPSA) has been used to bind human prostate-specific antigen (hPSA), a model cancer used in this study. Ellipsometry was used to characterize and optimise the antibody adsorption and antigen binding on gold surface. It was found that the best amount of antibody at the gold surface for effective antigen binding is around 1mg/m2, above or below which resulted in the reduced antigen binding due to either the limited binding sites (below 1mg/m2) or increased steric effect (above 1mg/m2). The FBAR data were in good agreement with the data obtained from ellipsometry. Antigen binding experiments using FBAR sensors demonstrated that FBARs have the capability to precisely detect antigen binding, thereby making FBARs an attractive low cost alternative to existing cancer diagnostic sensors. |
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title_short |
Label-free detection of human prostate-specific antigen (hPSA) using film bulk acoustic resonators (FBARs) |
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