Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout
A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Ma, Xiaoming [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014transfer abstract |
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| Schlagwörter: |
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| Umfang: |
5 |
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| Übergeordnetes Werk: |
Enthalten in: Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines - Costanigro, Marco ELSEVIER, 2019, the principal international journal devoted to research, design development and application of biosensors and bioelectronics, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:55 ; year:2014 ; day:15 ; month:05 ; pages:412-416 ; extent:5 |
| Links: |
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| DOI / URN: |
10.1016/j.bios.2013.12.041 |
|---|
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| 520 | |a A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. | ||
| 520 | |a A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. | ||
| 650 | 7 | |a Biosensor |2 Elsevier | |
| 650 | 7 | |a Platelet-derived growth factor B |2 Elsevier | |
| 650 | 7 | |a Personal glucose meters |2 Elsevier | |
| 650 | 7 | |a Aptamer |2 Elsevier | |
| 700 | 1 | |a Chen, Zhonghui |4 oth | |
| 700 | 1 | |a Zhou, Jin |4 oth | |
| 700 | 1 | |a Weng, Wen |4 oth | |
| 700 | 1 | |a Zheng, Ou |4 oth | |
| 700 | 1 | |a Lin, Zhenyu |4 oth | |
| 700 | 1 | |a Guo, Longhua |4 oth | |
| 700 | 1 | |a Qiu, Bin |4 oth | |
| 700 | 1 | |a Chen, Guonan |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Costanigro, Marco ELSEVIER |t Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines |d 2019 |d the principal international journal devoted to research, design development and application of biosensors and bioelectronics |g Amsterdam [u.a.] |w (DE-627)ELV001931067 |
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10.1016/j.bios.2013.12.041 doi GBVA2014016000017.pica (DE-627)ELV012519413 (ELSEVIER)S0956-5663(13)00912-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Ma, Xiaoming verfasserin aut Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout 2014transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. Biosensor Elsevier Platelet-derived growth factor B Elsevier Personal glucose meters Elsevier Aptamer Elsevier Chen, Zhonghui oth Zhou, Jin oth Weng, Wen oth Zheng, Ou oth Lin, Zhenyu oth Guo, Longhua oth Qiu, Bin oth Chen, Guonan oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:55 year:2014 day:15 month:05 pages:412-416 extent:5 https://doi.org/10.1016/j.bios.2013.12.041 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 55 2014 15 0515 412-416 5 045F 570 |
| spelling |
10.1016/j.bios.2013.12.041 doi GBVA2014016000017.pica (DE-627)ELV012519413 (ELSEVIER)S0956-5663(13)00912-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Ma, Xiaoming verfasserin aut Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout 2014transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. Biosensor Elsevier Platelet-derived growth factor B Elsevier Personal glucose meters Elsevier Aptamer Elsevier Chen, Zhonghui oth Zhou, Jin oth Weng, Wen oth Zheng, Ou oth Lin, Zhenyu oth Guo, Longhua oth Qiu, Bin oth Chen, Guonan oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:55 year:2014 day:15 month:05 pages:412-416 extent:5 https://doi.org/10.1016/j.bios.2013.12.041 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 55 2014 15 0515 412-416 5 045F 570 |
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10.1016/j.bios.2013.12.041 doi GBVA2014016000017.pica (DE-627)ELV012519413 (ELSEVIER)S0956-5663(13)00912-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Ma, Xiaoming verfasserin aut Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout 2014transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. Biosensor Elsevier Platelet-derived growth factor B Elsevier Personal glucose meters Elsevier Aptamer Elsevier Chen, Zhonghui oth Zhou, Jin oth Weng, Wen oth Zheng, Ou oth Lin, Zhenyu oth Guo, Longhua oth Qiu, Bin oth Chen, Guonan oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:55 year:2014 day:15 month:05 pages:412-416 extent:5 https://doi.org/10.1016/j.bios.2013.12.041 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 55 2014 15 0515 412-416 5 045F 570 |
| allfieldsGer |
10.1016/j.bios.2013.12.041 doi GBVA2014016000017.pica (DE-627)ELV012519413 (ELSEVIER)S0956-5663(13)00912-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Ma, Xiaoming verfasserin aut Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout 2014transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. Biosensor Elsevier Platelet-derived growth factor B Elsevier Personal glucose meters Elsevier Aptamer Elsevier Chen, Zhonghui oth Zhou, Jin oth Weng, Wen oth Zheng, Ou oth Lin, Zhenyu oth Guo, Longhua oth Qiu, Bin oth Chen, Guonan oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:55 year:2014 day:15 month:05 pages:412-416 extent:5 https://doi.org/10.1016/j.bios.2013.12.041 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 55 2014 15 0515 412-416 5 045F 570 |
| allfieldsSound |
10.1016/j.bios.2013.12.041 doi GBVA2014016000017.pica (DE-627)ELV012519413 (ELSEVIER)S0956-5663(13)00912-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Ma, Xiaoming verfasserin aut Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout 2014transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. Biosensor Elsevier Platelet-derived growth factor B Elsevier Personal glucose meters Elsevier Aptamer Elsevier Chen, Zhonghui oth Zhou, Jin oth Weng, Wen oth Zheng, Ou oth Lin, Zhenyu oth Guo, Longhua oth Qiu, Bin oth Chen, Guonan oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:55 year:2014 day:15 month:05 pages:412-416 extent:5 https://doi.org/10.1016/j.bios.2013.12.041 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 55 2014 15 0515 412-416 5 045F 570 |
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Enthalten in Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines Amsterdam [u.a.] volume:55 year:2014 day:15 month:05 pages:412-416 extent:5 |
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Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout |
| abstract |
A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. |
| abstractGer |
A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. |
| abstract_unstemmed |
A novel portable biosensor for sensitive and selective detection of platelet-derived growth factor-BB (PDGF-BB) had been developed based on the specific recognition between aptamer and protein using a personal glucose meter (PGM) as readout. In the presence of PDGF-BB, the primary aptamer of PDGF-BB which is bound to the surface of streptavidin magnespheres paramagnetic particles (SA-PMPs) reacts quantitatively with invertase-functionalized secondary aptamer of PDGF-BB to form a stable complex, resulting in the attachment of invertase on the SA-PMPs. Subsequently, the invertase catalyzes the hydrolysis of sucrose to produce a large amount of glucose and quantitative readout by the PGM. The enhanced signal of the PGM has a relationship with the concentration of PDGF-BB in the range of 1.0×10–14 M~3.16×10−12 M, and the detection limit is 2.9fM. The proposed portable biosensor had been successfully applied to assay the PDGF-BB in saliva samples. |
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Aptamer-based portable biosensor for platelet-derived growth factor-BB (PDGF-BB) with personal glucose meter readout |
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Chen, Zhonghui Zhou, Jin Weng, Wen Zheng, Ou Lin, Zhenyu Guo, Longhua Qiu, Bin Chen, Guonan |
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