A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues
A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. E...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wang, Peilong [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015transfer abstract |
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| Schlagwörter: |
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| Umfang: |
6 |
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| Übergeordnetes Werk: |
Enthalten in: Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines - Costanigro, Marco ELSEVIER, 2019, the principal international journal devoted to research, design development and application of biosensors and bioelectronics, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:64 ; year:2015 ; day:15 ; month:02 ; pages:511-516 ; extent:6 |
| Links: |
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| DOI / URN: |
10.1016/j.bios.2014.09.064 |
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| 520 | |a A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. | ||
| 520 | |a A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. | ||
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| 700 | 1 | |a Wang, Zhi |4 oth | |
| 700 | 1 | |a Su, Xiaoou |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Costanigro, Marco ELSEVIER |t Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines |d 2019 |d the principal international journal devoted to research, design development and application of biosensors and bioelectronics |g Amsterdam [u.a.] |w (DE-627)ELV001931067 |
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10.1016/j.bios.2014.09.064 doi GBVA2015016000015.pica (DE-627)ELV013265555 (ELSEVIER)S0956-5663(14)00751-9 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. β-Agonist Elsevier Multi-analyte detection Elsevier Fluorescent bead Elsevier Immuno-chromatographic sensor Elsevier Wang, Zhi oth Su, Xiaoou oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:64 year:2015 day:15 month:02 pages:511-516 extent:6 https://doi.org/10.1016/j.bios.2014.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 64 2015 15 0215 511-516 6 045F 570 |
| spelling |
10.1016/j.bios.2014.09.064 doi GBVA2015016000015.pica (DE-627)ELV013265555 (ELSEVIER)S0956-5663(14)00751-9 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. β-Agonist Elsevier Multi-analyte detection Elsevier Fluorescent bead Elsevier Immuno-chromatographic sensor Elsevier Wang, Zhi oth Su, Xiaoou oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:64 year:2015 day:15 month:02 pages:511-516 extent:6 https://doi.org/10.1016/j.bios.2014.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 64 2015 15 0215 511-516 6 045F 570 |
| allfields_unstemmed |
10.1016/j.bios.2014.09.064 doi GBVA2015016000015.pica (DE-627)ELV013265555 (ELSEVIER)S0956-5663(14)00751-9 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. β-Agonist Elsevier Multi-analyte detection Elsevier Fluorescent bead Elsevier Immuno-chromatographic sensor Elsevier Wang, Zhi oth Su, Xiaoou oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:64 year:2015 day:15 month:02 pages:511-516 extent:6 https://doi.org/10.1016/j.bios.2014.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 64 2015 15 0215 511-516 6 045F 570 |
| allfieldsGer |
10.1016/j.bios.2014.09.064 doi GBVA2015016000015.pica (DE-627)ELV013265555 (ELSEVIER)S0956-5663(14)00751-9 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. β-Agonist Elsevier Multi-analyte detection Elsevier Fluorescent bead Elsevier Immuno-chromatographic sensor Elsevier Wang, Zhi oth Su, Xiaoou oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:64 year:2015 day:15 month:02 pages:511-516 extent:6 https://doi.org/10.1016/j.bios.2014.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 64 2015 15 0215 511-516 6 045F 570 |
| allfieldsSound |
10.1016/j.bios.2014.09.064 doi GBVA2015016000015.pica (DE-627)ELV013265555 (ELSEVIER)S0956-5663(14)00751-9 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. β-Agonist Elsevier Multi-analyte detection Elsevier Fluorescent bead Elsevier Immuno-chromatographic sensor Elsevier Wang, Zhi oth Su, Xiaoou oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:64 year:2015 day:15 month:02 pages:511-516 extent:6 https://doi.org/10.1016/j.bios.2014.09.064 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 64 2015 15 0215 511-516 6 045F 570 |
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Enthalten in Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines Amsterdam [u.a.] volume:64 year:2015 day:15 month:02 pages:511-516 extent:6 |
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Enthalten in Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines Amsterdam [u.a.] volume:64 year:2015 day:15 month:02 pages:511-516 extent:6 |
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A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. 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a sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues |
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A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues |
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A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. |
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A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. |
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A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor was developed for detection of three β-agonizts: clenbuterol, ractopamine and salbuterol. A competitive immune strategy between antibody conjugated fluorescent beads and β-agonist or their antigens was employed. Each monoclonal antibody specifically recognizes it is corresponding β-agonist in the conjugating zone. The unreacted antibodies were captured by β-agonist antigens immobilized at three test lines in nitrocellulose membrane reaction zone. This enables simultaneous detection of 3 β-agonizts in one single test without any further sample preparation. The test results can be obtained within 10min. Limit of detections for clenbuterol, ractopamine and salbuterol were 0.10ng/mL, 0.10ng/mL and 0.09ng/mL, respectively. Recoveries ranged from 70.0% to 100.5% and relative standard deviations were below 15%. The assay was evaluated using spiked and real samples and the results were compared with LC–MS/MS. The developed novel assay method provides a low cost, sensitive and rapid approach for on site detection of β-agonizts. |
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A sensitive and quantitative fluorescent multi-component immuno-chromatographic sensor for β-agonist residues |
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