Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol
A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wang, Peilong [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016transfer abstract |
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| Schlagwörter: |
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| Umfang: |
5 |
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| Übergeordnetes Werk: |
Enthalten in: Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines - Costanigro, Marco ELSEVIER, 2019, the principal international journal devoted to research, design development and application of biosensors and bioelectronics, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:77 ; year:2016 ; day:15 ; month:03 ; pages:866-870 ; extent:5 |
| Links: |
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| DOI / URN: |
10.1016/j.bios.2015.10.053 |
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ELV014372762 |
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| 245 | 1 | 0 | |a Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol |
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| 520 | |a A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. | ||
| 520 | |a A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. | ||
| 650 | 7 | |a Immunochromatographic sensor |2 Elsevier | |
| 650 | 7 | |a Clenbuterol |2 Elsevier | |
| 650 | 7 | |a Up conversion phosphors |2 Elsevier | |
| 700 | 1 | |a Wang, Ruiguo |4 oth | |
| 700 | 1 | |a Zhang, Wei |4 oth | |
| 700 | 1 | |a Su, Xiaoou |4 oth | |
| 700 | 1 | |a Luo, Haifeng |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Costanigro, Marco ELSEVIER |t Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines |d 2019 |d the principal international journal devoted to research, design development and application of biosensors and bioelectronics |g Amsterdam [u.a.] |w (DE-627)ELV001931067 |
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10.1016/j.bios.2015.10.053 doi GBVA2016016000025.pica (DE-627)ELV014372762 (ELSEVIER)S0956-5663(15)30515-7 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol 2016transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. Immunochromatographic sensor Elsevier Clenbuterol Elsevier Up conversion phosphors Elsevier Wang, Ruiguo oth Zhang, Wei oth Su, Xiaoou oth Luo, Haifeng oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:77 year:2016 day:15 month:03 pages:866-870 extent:5 https://doi.org/10.1016/j.bios.2015.10.053 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 77 2016 15 0315 866-870 5 045F 570 |
| spelling |
10.1016/j.bios.2015.10.053 doi GBVA2016016000025.pica (DE-627)ELV014372762 (ELSEVIER)S0956-5663(15)30515-7 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol 2016transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. Immunochromatographic sensor Elsevier Clenbuterol Elsevier Up conversion phosphors Elsevier Wang, Ruiguo oth Zhang, Wei oth Su, Xiaoou oth Luo, Haifeng oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:77 year:2016 day:15 month:03 pages:866-870 extent:5 https://doi.org/10.1016/j.bios.2015.10.053 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 77 2016 15 0315 866-870 5 045F 570 |
| allfields_unstemmed |
10.1016/j.bios.2015.10.053 doi GBVA2016016000025.pica (DE-627)ELV014372762 (ELSEVIER)S0956-5663(15)30515-7 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol 2016transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. Immunochromatographic sensor Elsevier Clenbuterol Elsevier Up conversion phosphors Elsevier Wang, Ruiguo oth Zhang, Wei oth Su, Xiaoou oth Luo, Haifeng oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:77 year:2016 day:15 month:03 pages:866-870 extent:5 https://doi.org/10.1016/j.bios.2015.10.053 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 77 2016 15 0315 866-870 5 045F 570 |
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10.1016/j.bios.2015.10.053 doi GBVA2016016000025.pica (DE-627)ELV014372762 (ELSEVIER)S0956-5663(15)30515-7 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol 2016transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. Immunochromatographic sensor Elsevier Clenbuterol Elsevier Up conversion phosphors Elsevier Wang, Ruiguo oth Zhang, Wei oth Su, Xiaoou oth Luo, Haifeng oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:77 year:2016 day:15 month:03 pages:866-870 extent:5 https://doi.org/10.1016/j.bios.2015.10.053 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 77 2016 15 0315 866-870 5 045F 570 |
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10.1016/j.bios.2015.10.053 doi GBVA2016016000025.pica (DE-627)ELV014372762 (ELSEVIER)S0956-5663(15)30515-7 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Wang, Peilong verfasserin aut Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol 2016transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. Immunochromatographic sensor Elsevier Clenbuterol Elsevier Up conversion phosphors Elsevier Wang, Ruiguo oth Zhang, Wei oth Su, Xiaoou oth Luo, Haifeng oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:77 year:2016 day:15 month:03 pages:866-870 extent:5 https://doi.org/10.1016/j.bios.2015.10.053 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 77 2016 15 0315 866-870 5 045F 570 |
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Enthalten in Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines Amsterdam [u.a.] volume:77 year:2016 day:15 month:03 pages:866-870 extent:5 |
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Enthalten in Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines Amsterdam [u.a.] volume:77 year:2016 day:15 month:03 pages:866-870 extent:5 |
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Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines |
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Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines |
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novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol |
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Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol |
| abstract |
A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. |
| abstractGer |
A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. |
| abstract_unstemmed |
A novel and ultra sensitive immunochromatographic assay sensor (ICA) based on up conversion phosphor (UCP) for quantitative detection of clenbuterol (CL) was developed. Monoclonal antibody against CL was labeled with UCP beads. The detection strategy is based on competitive immunoreaction between CL antibodies conjugated to UCP beads and CL or CL antigen on the UCP-ICA sensor. It enables ultra sensitive detection of CL in one single test without complicated sample preparation. Sensing results can be obtained within 10min. Under optimized conditions, visual limit of detection (vLOD) of UCP-ICA for CL was 0.1ng/mL. Calculated LOD (cLOD) for CL, as low as 0.01ng/mL, could be achieved with the UCP-ICA sensor. Recoveries of CL in various sample matrixes ranged from 73.0% to 92.2% and relative standard deviations (RSD) were below 12%. The assay was evaluated with spiked and real samples and the results were compared with liquid chromatography-tandem mass. The developed novel assay method based on UCP could be a potential alternative format for on site and rapid detection of CL as well as other illegal drugs. |
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Novel fabrication of immunochromatographic assay based on up conversion phosphors for sensitive detection of clenbuterol |
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