An Electrochemical Immunosensor for Rapid and Sensitive Detection of Mycotoxins Fumonisin B1 and Deoxynivalenol
We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (A...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Lu, Lin [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2016transfer abstract |
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| Umfang: |
9 |
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| Übergeordnetes Werk: |
Enthalten in: Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch - Zhang, Lei ELSEVIER, 2018, the journal of the International Society of Electrochemistry (ISE), New York, NY [u.a.] |
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| Übergeordnetes Werk: |
volume:213 ; year:2016 ; day:20 ; month:09 ; pages:89-97 ; extent:9 |
| Links: |
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| DOI / URN: |
10.1016/j.electacta.2016.07.096 |
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| 245 | 1 | 0 | |a An Electrochemical Immunosensor for Rapid and Sensitive Detection of Mycotoxins Fumonisin B1 and Deoxynivalenol |
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| 520 | |a We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. | ||
| 520 | |a We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. | ||
| 650 | 7 | |a electrochemical immunosensing |2 Elsevier | |
| 650 | 7 | |a DON |2 Elsevier | |
| 650 | 7 | |a fumonisin |2 Elsevier | |
| 650 | 7 | |a reduced graphene oxide |2 Elsevier | |
| 650 | 7 | |a mycotoxins |2 Elsevier | |
| 700 | 1 | |a Seenivasan, Rajesh |4 oth | |
| 700 | 1 | |a Wang, Yi-Cheng |4 oth | |
| 700 | 1 | |a Yu, Jae-Hyuk |4 oth | |
| 700 | 1 | |a Gunasekaran, Sundaram |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Zhang, Lei ELSEVIER |t Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch |d 2018 |d the journal of the International Society of Electrochemistry (ISE) |g New York, NY [u.a.] |w (DE-627)ELV001212419 |
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10.1016/j.electacta.2016.07.096 doi GBV00000000000001.pica (DE-627)ELV014516535 (ELSEVIER)S0013-4686(16)31602-4 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 44.00 bkl Lu, Lin verfasserin aut An Electrochemical Immunosensor for Rapid and Sensitive Detection of Mycotoxins Fumonisin B1 and Deoxynivalenol 2016transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. electrochemical immunosensing Elsevier DON Elsevier fumonisin Elsevier reduced graphene oxide Elsevier mycotoxins Elsevier Seenivasan, Rajesh oth Wang, Yi-Cheng oth Yu, Jae-Hyuk oth Gunasekaran, Sundaram oth Enthalten in Elsevier Zhang, Lei ELSEVIER Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch 2018 the journal of the International Society of Electrochemistry (ISE) New York, NY [u.a.] (DE-627)ELV001212419 volume:213 year:2016 day:20 month:09 pages:89-97 extent:9 https://doi.org/10.1016/j.electacta.2016.07.096 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.00 Medizin: Allgemeines VZ AR 213 2016 20 0920 89-97 9 045F 540 |
| spelling |
10.1016/j.electacta.2016.07.096 doi GBV00000000000001.pica (DE-627)ELV014516535 (ELSEVIER)S0013-4686(16)31602-4 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 44.00 bkl Lu, Lin verfasserin aut An Electrochemical Immunosensor for Rapid and Sensitive Detection of Mycotoxins Fumonisin B1 and Deoxynivalenol 2016transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. electrochemical immunosensing Elsevier DON Elsevier fumonisin Elsevier reduced graphene oxide Elsevier mycotoxins Elsevier Seenivasan, Rajesh oth Wang, Yi-Cheng oth Yu, Jae-Hyuk oth Gunasekaran, Sundaram oth Enthalten in Elsevier Zhang, Lei ELSEVIER Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch 2018 the journal of the International Society of Electrochemistry (ISE) New York, NY [u.a.] (DE-627)ELV001212419 volume:213 year:2016 day:20 month:09 pages:89-97 extent:9 https://doi.org/10.1016/j.electacta.2016.07.096 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.00 Medizin: Allgemeines VZ AR 213 2016 20 0920 89-97 9 045F 540 |
| allfields_unstemmed |
10.1016/j.electacta.2016.07.096 doi GBV00000000000001.pica (DE-627)ELV014516535 (ELSEVIER)S0013-4686(16)31602-4 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 44.00 bkl Lu, Lin verfasserin aut An Electrochemical Immunosensor for Rapid and Sensitive Detection of Mycotoxins Fumonisin B1 and Deoxynivalenol 2016transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. electrochemical immunosensing Elsevier DON Elsevier fumonisin Elsevier reduced graphene oxide Elsevier mycotoxins Elsevier Seenivasan, Rajesh oth Wang, Yi-Cheng oth Yu, Jae-Hyuk oth Gunasekaran, Sundaram oth Enthalten in Elsevier Zhang, Lei ELSEVIER Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch 2018 the journal of the International Society of Electrochemistry (ISE) New York, NY [u.a.] (DE-627)ELV001212419 volume:213 year:2016 day:20 month:09 pages:89-97 extent:9 https://doi.org/10.1016/j.electacta.2016.07.096 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.00 Medizin: Allgemeines VZ AR 213 2016 20 0920 89-97 9 045F 540 |
| allfieldsGer |
10.1016/j.electacta.2016.07.096 doi GBV00000000000001.pica (DE-627)ELV014516535 (ELSEVIER)S0013-4686(16)31602-4 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 44.00 bkl Lu, Lin verfasserin aut An Electrochemical Immunosensor for Rapid and Sensitive Detection of Mycotoxins Fumonisin B1 and Deoxynivalenol 2016transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. electrochemical immunosensing Elsevier DON Elsevier fumonisin Elsevier reduced graphene oxide Elsevier mycotoxins Elsevier Seenivasan, Rajesh oth Wang, Yi-Cheng oth Yu, Jae-Hyuk oth Gunasekaran, Sundaram oth Enthalten in Elsevier Zhang, Lei ELSEVIER Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch 2018 the journal of the International Society of Electrochemistry (ISE) New York, NY [u.a.] (DE-627)ELV001212419 volume:213 year:2016 day:20 month:09 pages:89-97 extent:9 https://doi.org/10.1016/j.electacta.2016.07.096 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.00 Medizin: Allgemeines VZ AR 213 2016 20 0920 89-97 9 045F 540 |
| allfieldsSound |
10.1016/j.electacta.2016.07.096 doi GBV00000000000001.pica (DE-627)ELV014516535 (ELSEVIER)S0013-4686(16)31602-4 DE-627 ger DE-627 rakwb eng 540 540 DE-600 610 VZ 44.00 bkl Lu, Lin verfasserin aut An Electrochemical Immunosensor for Rapid and Sensitive Detection of Mycotoxins Fumonisin B1 and Deoxynivalenol 2016transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. electrochemical immunosensing Elsevier DON Elsevier fumonisin Elsevier reduced graphene oxide Elsevier mycotoxins Elsevier Seenivasan, Rajesh oth Wang, Yi-Cheng oth Yu, Jae-Hyuk oth Gunasekaran, Sundaram oth Enthalten in Elsevier Zhang, Lei ELSEVIER Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch 2018 the journal of the International Society of Electrochemistry (ISE) New York, NY [u.a.] (DE-627)ELV001212419 volume:213 year:2016 day:20 month:09 pages:89-97 extent:9 https://doi.org/10.1016/j.electacta.2016.07.096 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.00 Medizin: Allgemeines VZ AR 213 2016 20 0920 89-97 9 045F 540 |
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English |
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Enthalten in Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch New York, NY [u.a.] volume:213 year:2016 day:20 month:09 pages:89-97 extent:9 |
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Enthalten in Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch New York, NY [u.a.] volume:213 year:2016 day:20 month:09 pages:89-97 extent:9 |
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electrochemical immunosensing DON fumonisin reduced graphene oxide mycotoxins |
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Computed tomographic morphometric analysis of lateral inclination C1 pedicle screw for atlantoaxial instability patients with a narrow C1 posterior arch |
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an electrochemical immunosensor for rapid and sensitive detection of mycotoxins fumonisin b1 and deoxynivalenol |
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An Electrochemical Immunosensor for Rapid and Sensitive Detection of Mycotoxins Fumonisin B1 and Deoxynivalenol |
| abstract |
We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. |
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We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. |
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We report an electrochemical immunosensing method for rapid and sensitive detection of two mycotoxins, fumonisin B1 (FB1) and deoxynivalenol (DON). A disposable screen-printed carbon electrode (SPE) was used as sensing platform. The working electrode part of SPE was modified by gold nanoparticles (AuNPs) and polypyrrole (PPy)-electrochemically reduced graphene oxide (ErGO) nanocomposite film for effective anti-toxin antibody immobilization, enhanced electrical conductivity, and biocompatibility. Under optimized test conditions, the limit of detection and linear range achieved for FB1 were 4.2ppb and 0.2 to 4.5ppm (%RSD=4.9%); and the corresponding values for DON were 8.6ppb and 0.05 to 1ppm (%RSD=5.7%). The immunosensor can specifically detect the target toxin in co-existing toxins environment. The sensor exhibited high sensitivity and low matrix interference when tested using extracts obtained from spiked corn samples. Hence, our electrochemical immunosensing scheme can be adopted for highly sensitive and rapid detection of multiple co-contaminant mycotoxins in food and feed products. |
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An Electrochemical Immunosensor for Rapid and Sensitive Detection of Mycotoxins Fumonisin B1 and Deoxynivalenol |
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