Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen
Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and car...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Xiao, Kun [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
|---|
| Schlagwörter: |
|---|
| Umfang: |
7 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications - Mohamed, S.H. ELSEVIER, 2019, the international journal of pure and applied analytical chemistry, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:164 ; year:2017 ; day:1 ; month:03 ; pages:463-469 ; extent:7 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.talanta.2016.12.003 |
|---|
| Katalog-ID: |
ELV014736349 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | ELV014736349 | ||
| 003 | DE-627 | ||
| 005 | 20230625113905.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180602s2017 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.talanta.2016.12.003 |2 doi | |
| 028 | 5 | 2 | |a GBV00000000000058A.pica |
| 035 | |a (DE-627)ELV014736349 | ||
| 035 | |a (ELSEVIER)S0039-9140(16)30949-3 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 540 | |
| 082 | 0 | 4 | |a 540 |q DE-600 |
| 082 | 0 | 4 | |a 530 |a 620 |q VZ |
| 084 | |a 53.56 |2 bkl | ||
| 100 | 1 | |a Xiao, Kun |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen |
| 264 | 1 | |c 2017transfer abstract | |
| 300 | |a 7 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. | ||
| 520 | |a Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. | ||
| 650 | 7 | |a PBS |2 Elsevier | |
| 650 | 7 | |a NSE |2 Elsevier | |
| 650 | 7 | |a QDs |2 Elsevier | |
| 650 | 7 | |a SCLC |2 Elsevier | |
| 650 | 7 | |a CA72-4 |2 Elsevier | |
| 650 | 7 | |a QBs |2 Elsevier | |
| 650 | 7 | |a EDC |2 Elsevier | |
| 650 | 7 | |a AFP |2 Elsevier | |
| 650 | 7 | |a BSA |2 Elsevier | |
| 650 | 7 | |a NC |2 Elsevier | |
| 650 | 7 | |a NHS |2 Elsevier | |
| 650 | 7 | |a ICTS |2 Elsevier | |
| 650 | 7 | |a C line |2 Elsevier | |
| 650 | 7 | |a PVC |2 Elsevier | |
| 650 | 7 | |a T1 |2 Elsevier | |
| 650 | 7 | |a CEA |2 Elsevier | |
| 650 | 7 | |a T2 |2 Elsevier | |
| 650 | 7 | |a PSA |2 Elsevier | |
| 700 | 1 | |a Wang, Kan |4 oth | |
| 700 | 1 | |a Qin, Weijian |4 oth | |
| 700 | 1 | |a Hou, Yafei |4 oth | |
| 700 | 1 | |a Lu, Wenting |4 oth | |
| 700 | 1 | |a Xu, Hao |4 oth | |
| 700 | 1 | |a Wo, Yan |4 oth | |
| 700 | 1 | |a Cui, Daxiang |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Mohamed, S.H. ELSEVIER |t Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |d 2019 |d the international journal of pure and applied analytical chemistry |g Amsterdam [u.a.] |w (DE-627)ELV003060667 |
| 773 | 1 | 8 | |g volume:164 |g year:2017 |g day:1 |g month:03 |g pages:463-469 |g extent:7 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.talanta.2016.12.003 |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a GBV_ELV | ||
| 912 | |a SYSFLAG_U | ||
| 936 | b | k | |a 53.56 |j Halbleitertechnologie |q VZ |
| 951 | |a AR | ||
| 952 | |d 164 |j 2017 |b 1 |c 0301 |h 463-469 |g 7 | ||
| 953 | |2 045F |a 540 | ||
| author_variant |
k x kx |
|---|---|
| matchkey_str |
xiaokunwangkanqinweijianhouyafeiluwentin:2017----:soqatmobasaeemncoaatbdtipoehsniiiyfqatttvadiutnosmuohoaorpiasyon |
| hierarchy_sort_str |
2017transfer abstract |
| bklnumber |
53.56 |
| publishDate |
2017 |
| allfields |
10.1016/j.talanta.2016.12.003 doi GBV00000000000058A.pica (DE-627)ELV014736349 (ELSEVIER)S0039-9140(16)30949-3 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Xiao, Kun verfasserin aut Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA Elsevier Wang, Kan oth Qin, Weijian oth Hou, Yafei oth Lu, Wenting oth Xu, Hao oth Wo, Yan oth Cui, Daxiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:164 year:2017 day:1 month:03 pages:463-469 extent:7 https://doi.org/10.1016/j.talanta.2016.12.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 164 2017 1 0301 463-469 7 045F 540 |
| spelling |
10.1016/j.talanta.2016.12.003 doi GBV00000000000058A.pica (DE-627)ELV014736349 (ELSEVIER)S0039-9140(16)30949-3 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Xiao, Kun verfasserin aut Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA Elsevier Wang, Kan oth Qin, Weijian oth Hou, Yafei oth Lu, Wenting oth Xu, Hao oth Wo, Yan oth Cui, Daxiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:164 year:2017 day:1 month:03 pages:463-469 extent:7 https://doi.org/10.1016/j.talanta.2016.12.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 164 2017 1 0301 463-469 7 045F 540 |
| allfields_unstemmed |
10.1016/j.talanta.2016.12.003 doi GBV00000000000058A.pica (DE-627)ELV014736349 (ELSEVIER)S0039-9140(16)30949-3 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Xiao, Kun verfasserin aut Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA Elsevier Wang, Kan oth Qin, Weijian oth Hou, Yafei oth Lu, Wenting oth Xu, Hao oth Wo, Yan oth Cui, Daxiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:164 year:2017 day:1 month:03 pages:463-469 extent:7 https://doi.org/10.1016/j.talanta.2016.12.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 164 2017 1 0301 463-469 7 045F 540 |
| allfieldsGer |
10.1016/j.talanta.2016.12.003 doi GBV00000000000058A.pica (DE-627)ELV014736349 (ELSEVIER)S0039-9140(16)30949-3 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Xiao, Kun verfasserin aut Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA Elsevier Wang, Kan oth Qin, Weijian oth Hou, Yafei oth Lu, Wenting oth Xu, Hao oth Wo, Yan oth Cui, Daxiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:164 year:2017 day:1 month:03 pages:463-469 extent:7 https://doi.org/10.1016/j.talanta.2016.12.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 164 2017 1 0301 463-469 7 045F 540 |
| allfieldsSound |
10.1016/j.talanta.2016.12.003 doi GBV00000000000058A.pica (DE-627)ELV014736349 (ELSEVIER)S0039-9140(16)30949-3 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Xiao, Kun verfasserin aut Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA Elsevier Wang, Kan oth Qin, Weijian oth Hou, Yafei oth Lu, Wenting oth Xu, Hao oth Wo, Yan oth Cui, Daxiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:164 year:2017 day:1 month:03 pages:463-469 extent:7 https://doi.org/10.1016/j.talanta.2016.12.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 164 2017 1 0301 463-469 7 045F 540 |
| language |
English |
| source |
Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:164 year:2017 day:1 month:03 pages:463-469 extent:7 |
| sourceStr |
Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:164 year:2017 day:1 month:03 pages:463-469 extent:7 |
| format_phy_str_mv |
Article |
| bklname |
Halbleitertechnologie |
| institution |
findex.gbv.de |
| topic_facet |
PBS NSE QDs SCLC CA72-4 QBs EDC AFP BSA NC NHS ICTS C line PVC T1 CEA T2 PSA |
| dewey-raw |
540 |
| isfreeaccess_bool |
false |
| container_title |
Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |
| authorswithroles_txt_mv |
Xiao, Kun @@aut@@ Wang, Kan @@oth@@ Qin, Weijian @@oth@@ Hou, Yafei @@oth@@ Lu, Wenting @@oth@@ Xu, Hao @@oth@@ Wo, Yan @@oth@@ Cui, Daxiang @@oth@@ |
| publishDateDaySort_date |
2017-01-01T00:00:00Z |
| hierarchy_top_id |
ELV003060667 |
| dewey-sort |
3540 |
| id |
ELV014736349 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV014736349</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230625113905.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180602s2017 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.talanta.2016.12.003</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBV00000000000058A.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV014736349</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0039-9140(16)30949-3</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">540</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">530</subfield><subfield code="a">620</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">53.56</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Xiao, Kun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2017transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">PBS</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">NSE</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">QDs</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">SCLC</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">CA72-4</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">QBs</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">EDC</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">AFP</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">BSA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">NC</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">NHS</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">ICTS</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">C line</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">PVC</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">T1</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">CEA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">T2</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">PSA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Kan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Qin, Weijian</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hou, Yafei</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Wenting</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Hao</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wo, Yan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cui, Daxiang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Mohamed, S.H. ELSEVIER</subfield><subfield code="t">Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications</subfield><subfield code="d">2019</subfield><subfield code="d">the international journal of pure and applied analytical chemistry</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV003060667</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:164</subfield><subfield code="g">year:2017</subfield><subfield code="g">day:1</subfield><subfield code="g">month:03</subfield><subfield code="g">pages:463-469</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.talanta.2016.12.003</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">53.56</subfield><subfield code="j">Halbleitertechnologie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">164</subfield><subfield code="j">2017</subfield><subfield code="b">1</subfield><subfield code="c">0301</subfield><subfield code="h">463-469</subfield><subfield code="g">7</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">540</subfield></datafield></record></collection>
|
| author |
Xiao, Kun |
| spellingShingle |
Xiao, Kun ddc 540 ddc 530 bkl 53.56 Elsevier PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen |
| authorStr |
Xiao, Kun |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)ELV003060667 |
| format |
electronic Article |
| dewey-ones |
540 - Chemistry & allied sciences 530 - Physics 620 - Engineering & allied operations |
| delete_txt_mv |
keep |
| author_role |
aut |
| collection |
elsevier |
| remote_str |
true |
| illustrated |
Not Illustrated |
| topic_title |
540 540 DE-600 530 620 VZ 53.56 bkl Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA Elsevier |
| topic |
ddc 540 ddc 530 bkl 53.56 Elsevier PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA |
| topic_unstemmed |
ddc 540 ddc 530 bkl 53.56 Elsevier PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA |
| topic_browse |
ddc 540 ddc 530 bkl 53.56 Elsevier PBS Elsevier NSE Elsevier QDs Elsevier SCLC Elsevier CA72-4 Elsevier QBs Elsevier EDC Elsevier AFP Elsevier BSA Elsevier NC Elsevier NHS Elsevier ICTS Elsevier C line Elsevier PVC Elsevier T1 Elsevier CEA Elsevier T2 Elsevier PSA |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
k w kw w q wq y h yh w l wl h x hx y w yw d c dc |
| hierarchy_parent_title |
Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |
| hierarchy_parent_id |
ELV003060667 |
| dewey-tens |
540 - Chemistry 530 - Physics 620 - Engineering |
| hierarchy_top_title |
Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)ELV003060667 |
| title |
Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen |
| ctrlnum |
(DE-627)ELV014736349 (ELSEVIER)S0039-9140(16)30949-3 |
| title_full |
Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen |
| author_sort |
Xiao, Kun |
| journal |
Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |
| journalStr |
Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
500 - Science 600 - Technology |
| recordtype |
marc |
| publishDateSort |
2017 |
| contenttype_str_mv |
zzz |
| container_start_page |
463 |
| author_browse |
Xiao, Kun |
| container_volume |
164 |
| physical |
7 |
| class |
540 540 DE-600 530 620 VZ 53.56 bkl |
| format_se |
Elektronische Aufsätze |
| author-letter |
Xiao, Kun |
| doi_str_mv |
10.1016/j.talanta.2016.12.003 |
| dewey-full |
540 530 620 |
| title_sort |
use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen |
| title_auth |
Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen |
| abstract |
Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. |
| abstractGer |
Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. |
| abstract_unstemmed |
Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis. |
| collection_details |
GBV_USEFLAG_U GBV_ELV SYSFLAG_U |
| title_short |
Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen |
| url |
https://doi.org/10.1016/j.talanta.2016.12.003 |
| remote_bool |
true |
| author2 |
Wang, Kan Qin, Weijian Hou, Yafei Lu, Wenting Xu, Hao Wo, Yan Cui, Daxiang |
| author2Str |
Wang, Kan Qin, Weijian Hou, Yafei Lu, Wenting Xu, Hao Wo, Yan Cui, Daxiang |
| ppnlink |
ELV003060667 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth oth oth |
| doi_str |
10.1016/j.talanta.2016.12.003 |
| up_date |
2024-07-06T22:18:46.150Z |
| _version_ |
1803869829099159552 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV014736349</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230625113905.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180602s2017 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.talanta.2016.12.003</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBV00000000000058A.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV014736349</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0039-9140(16)30949-3</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">540</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">530</subfield><subfield code="a">620</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">53.56</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Xiao, Kun</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Use of quantum dot beads-labeled monoclonal antibody to improve the sensitivity of a quantitative and simultaneous immunochromatographic assay for neuron specific enolase and carcinoembryonic antigen</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2017transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">7</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Detection of multiplex tumor markers was of great importance for cancer diagnosis. Immunochromatographic test strip (ICTS) was the most frequently-used point-of-care detection means. Herein, a convenient and fast method for simultaneous quantitative detection of neuron specific enolase (NSE) and carcinoembryonic antigen (CEA) was developed based on ICTS using quantum dot beads (QBs) as marking material. Good monodispersity, high colloidal stability and carboxyl-modified (COOH–) QBs were used. For this method, two test lines were applied to the NC membrane for simultaneous analysis of CEA and NSE respectively. The ideal limit of CEA and NSE detection was 0.0378ng/mL and 0.0426ng/mL with scarcely any cross-reactivity. Moreover, the fluorescent signal intensity of the nitrocellulose membrane could be easily read out in the cooperation of the “Handing” system without professional operators. The possible clinical utilization of this platform was demonstrated by detecting 100 clinic human serums. The result showed that the platform had sensitivity of 99% and 97% for CEA and NSE, while the specificity was 97% and 100% respectively. Our results indicated that the QBs based ICTS not only owning the ability of sensitive and specific simultaneous detection of CEA and NSE, but also showing the potential in developing this ICTS into a routine part of early lung cancer diagnosis.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">PBS</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">NSE</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">QDs</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">SCLC</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">CA72-4</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">QBs</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">EDC</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">AFP</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">BSA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">NC</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">NHS</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">ICTS</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">C line</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">PVC</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">T1</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">CEA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">T2</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">PSA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wang, Kan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Qin, Weijian</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Hou, Yafei</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Wenting</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xu, Hao</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wo, Yan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Cui, Daxiang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Mohamed, S.H. ELSEVIER</subfield><subfield code="t">Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications</subfield><subfield code="d">2019</subfield><subfield code="d">the international journal of pure and applied analytical chemistry</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV003060667</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:164</subfield><subfield code="g">year:2017</subfield><subfield code="g">day:1</subfield><subfield code="g">month:03</subfield><subfield code="g">pages:463-469</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.talanta.2016.12.003</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">53.56</subfield><subfield code="j">Halbleitertechnologie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">164</subfield><subfield code="j">2017</subfield><subfield code="b">1</subfield><subfield code="c">0301</subfield><subfield code="h">463-469</subfield><subfield code="g">7</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">540</subfield></datafield></record></collection>
|
| score |
7.401638 |