L-noradrenaline functionalized near-infrared fluorescence CdSeTe probe for the determination of urea and bioimaging of HepG2 Cells
In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quino...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Li, Zhenzhen [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
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| Umfang: |
9 |
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| Übergeordnetes Werk: |
Enthalten in: Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications - Mohamed, S.H. ELSEVIER, 2019, the international journal of pure and applied analytical chemistry, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:171 ; year:2017 ; day:15 ; month:08 ; pages:16-24 ; extent:9 |
| Links: |
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| DOI / URN: |
10.1016/j.talanta.2017.04.003 |
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ELV014739968 |
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| 520 | |a In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. | ||
| 520 | |a In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. | ||
| 650 | 7 | |a NIR QDs |2 Elsevier | |
| 650 | 7 | |a NIR bioimaging |2 Elsevier | |
| 650 | 7 | |a NIR probe |2 Elsevier | |
| 650 | 7 | |a Urea detection |2 Elsevier | |
| 650 | 7 | |a Fluorescence quenching |2 Elsevier | |
| 700 | 1 | |a Zhang, Qiyi |4 oth | |
| 700 | 1 | |a Huang, Huaying |4 oth | |
| 700 | 1 | |a Ren, Changjing |4 oth | |
| 700 | 1 | |a Ouyang, Si |4 oth | |
| 700 | 1 | |a Zhao, Qiang |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Mohamed, S.H. ELSEVIER |t Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |d 2019 |d the international journal of pure and applied analytical chemistry |g Amsterdam [u.a.] |w (DE-627)ELV003060667 |
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10.1016/j.talanta.2017.04.003 doi GBVA2017001000010.pica (DE-627)ELV014739968 (ELSEVIER)S0039-9140(17)30418-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Li, Zhenzhen verfasserin aut L-noradrenaline functionalized near-infrared fluorescence CdSeTe probe for the determination of urea and bioimaging of HepG2 Cells 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. NIR QDs Elsevier NIR bioimaging Elsevier NIR probe Elsevier Urea detection Elsevier Fluorescence quenching Elsevier Zhang, Qiyi oth Huang, Huaying oth Ren, Changjing oth Ouyang, Si oth Zhao, Qiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:171 year:2017 day:15 month:08 pages:16-24 extent:9 https://doi.org/10.1016/j.talanta.2017.04.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 171 2017 15 0815 16-24 9 045F 540 |
| spelling |
10.1016/j.talanta.2017.04.003 doi GBVA2017001000010.pica (DE-627)ELV014739968 (ELSEVIER)S0039-9140(17)30418-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Li, Zhenzhen verfasserin aut L-noradrenaline functionalized near-infrared fluorescence CdSeTe probe for the determination of urea and bioimaging of HepG2 Cells 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. NIR QDs Elsevier NIR bioimaging Elsevier NIR probe Elsevier Urea detection Elsevier Fluorescence quenching Elsevier Zhang, Qiyi oth Huang, Huaying oth Ren, Changjing oth Ouyang, Si oth Zhao, Qiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:171 year:2017 day:15 month:08 pages:16-24 extent:9 https://doi.org/10.1016/j.talanta.2017.04.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 171 2017 15 0815 16-24 9 045F 540 |
| allfields_unstemmed |
10.1016/j.talanta.2017.04.003 doi GBVA2017001000010.pica (DE-627)ELV014739968 (ELSEVIER)S0039-9140(17)30418-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Li, Zhenzhen verfasserin aut L-noradrenaline functionalized near-infrared fluorescence CdSeTe probe for the determination of urea and bioimaging of HepG2 Cells 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. NIR QDs Elsevier NIR bioimaging Elsevier NIR probe Elsevier Urea detection Elsevier Fluorescence quenching Elsevier Zhang, Qiyi oth Huang, Huaying oth Ren, Changjing oth Ouyang, Si oth Zhao, Qiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:171 year:2017 day:15 month:08 pages:16-24 extent:9 https://doi.org/10.1016/j.talanta.2017.04.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 171 2017 15 0815 16-24 9 045F 540 |
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10.1016/j.talanta.2017.04.003 doi GBVA2017001000010.pica (DE-627)ELV014739968 (ELSEVIER)S0039-9140(17)30418-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Li, Zhenzhen verfasserin aut L-noradrenaline functionalized near-infrared fluorescence CdSeTe probe for the determination of urea and bioimaging of HepG2 Cells 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. NIR QDs Elsevier NIR bioimaging Elsevier NIR probe Elsevier Urea detection Elsevier Fluorescence quenching Elsevier Zhang, Qiyi oth Huang, Huaying oth Ren, Changjing oth Ouyang, Si oth Zhao, Qiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:171 year:2017 day:15 month:08 pages:16-24 extent:9 https://doi.org/10.1016/j.talanta.2017.04.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 171 2017 15 0815 16-24 9 045F 540 |
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10.1016/j.talanta.2017.04.003 doi GBVA2017001000010.pica (DE-627)ELV014739968 (ELSEVIER)S0039-9140(17)30418-6 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Li, Zhenzhen verfasserin aut L-noradrenaline functionalized near-infrared fluorescence CdSeTe probe for the determination of urea and bioimaging of HepG2 Cells 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. NIR QDs Elsevier NIR bioimaging Elsevier NIR probe Elsevier Urea detection Elsevier Fluorescence quenching Elsevier Zhang, Qiyi oth Huang, Huaying oth Ren, Changjing oth Ouyang, Si oth Zhao, Qiang oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:171 year:2017 day:15 month:08 pages:16-24 extent:9 https://doi.org/10.1016/j.talanta.2017.04.003 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 171 2017 15 0815 16-24 9 045F 540 |
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Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:171 year:2017 day:15 month:08 pages:16-24 extent:9 |
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Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:171 year:2017 day:15 month:08 pages:16-24 extent:9 |
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Li, Zhenzhen ddc 540 ddc 530 bkl 53.56 Elsevier NIR QDs Elsevier NIR bioimaging Elsevier NIR probe Elsevier Urea detection Elsevier Fluorescence quenching L-noradrenaline functionalized near-infrared fluorescence CdSeTe probe for the determination of urea and bioimaging of HepG2 Cells |
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l-noradrenaline functionalized near-infrared fluorescence cdsete probe for the determination of urea and bioimaging of hepg2 cells |
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L-noradrenaline functionalized near-infrared fluorescence CdSeTe probe for the determination of urea and bioimaging of HepG2 Cells |
| abstract |
In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. |
| abstractGer |
In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. |
| abstract_unstemmed |
In this paper, near-infrared (NIR) light emitting L-noradrenaline functionalized CdSeTe QDs (NA-CdSeTe) were synthesized and applied to the biosensing of urea. When the pH value of NA-CdSeTe solution was adjusted from neutral to alkalinity, the noradrenaline on the surface of QDs would turn to quinone, which triggered the fluorescence quenching of NA-CdSeTe probe due to the charge transfer interactions between QDs and the proximal quinone. Based on the fact that the hydrolysis of urea in the presence of urease would release OH− and slightly change the pH value of the solution, the fluorescence intensity of NA-CdSeTe QDs could be linked to the enzymatic degradation of urea. The novel urea-biosensing system could effectively determinate urea in the dynamic concentration range from 0.057 to 13mmol/L. Furthermore, NA-CdSeTe probe could serve as an “optical window” for the bioimaging of urea with high selectivity in the serum samples and HepG2 cells. The urea-bioimaging system could effectively probe urea in the dynamic concentration range from 0.2 to 5mmol/L. This NIR probe was excellent candidate not only for its sensitive with urea but also its real-time bioimaging. We expected that this NIR probe based strategy could pave the way for developing simple, no enzyme immobilization required and good sensitive method related detections for further medical applications. |
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