Combined micro-XRF and TXRF methodology for quantitative elemental imaging of tissue samples
Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the d...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Wróbel, Paweł M. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
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| Schlagwörter: |
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| Umfang: |
6 |
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| Übergeordnetes Werk: |
Enthalten in: Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications - Mohamed, S.H. ELSEVIER, 2019, the international journal of pure and applied analytical chemistry, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:162 ; year:2017 ; day:1 ; month:01 ; pages:654-659 ; extent:6 |
| Links: |
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| DOI / URN: |
10.1016/j.talanta.2016.10.043 |
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ELV01474080X |
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| 245 | 1 | 0 | |a Combined micro-XRF and TXRF methodology for quantitative elemental imaging of tissue samples |
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| 520 | |a Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. | ||
| 520 | |a Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. | ||
| 650 | 7 | |a Spleen |2 Elsevier | |
| 650 | 7 | |a Liver |2 Elsevier | |
| 650 | 7 | |a Micro-XRF |2 Elsevier | |
| 650 | 7 | |a Elemental imaging |2 Elsevier | |
| 650 | 7 | |a Matrix effects |2 Elsevier | |
| 650 | 7 | |a Kidney |2 Elsevier | |
| 650 | 7 | |a TXRF |2 Elsevier | |
| 700 | 1 | |a Bała, Sławomir |4 oth | |
| 700 | 1 | |a Czyzycki, Mateusz |4 oth | |
| 700 | 1 | |a Golasik, Magdalena |4 oth | |
| 700 | 1 | |a Librowski, Tadeusz |4 oth | |
| 700 | 1 | |a Ostachowicz, Beata |4 oth | |
| 700 | 1 | |a Piekoszewski, Wojciech |4 oth | |
| 700 | 1 | |a Surówka, Artur |4 oth | |
| 700 | 1 | |a Lankosz, Marek |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Mohamed, S.H. ELSEVIER |t Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |d 2019 |d the international journal of pure and applied analytical chemistry |g Amsterdam [u.a.] |w (DE-627)ELV003060667 |
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10.1016/j.talanta.2016.10.043 doi GBVA2017001000010.pica (DE-627)ELV01474080X (ELSEVIER)S0039-9140(16)30793-7 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Wróbel, Paweł M. verfasserin aut Combined micro-XRF and TXRF methodology for quantitative elemental imaging of tissue samples 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Spleen Elsevier Liver Elsevier Micro-XRF Elsevier Elemental imaging Elsevier Matrix effects Elsevier Kidney Elsevier TXRF Elsevier Bała, Sławomir oth Czyzycki, Mateusz oth Golasik, Magdalena oth Librowski, Tadeusz oth Ostachowicz, Beata oth Piekoszewski, Wojciech oth Surówka, Artur oth Lankosz, Marek oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:162 year:2017 day:1 month:01 pages:654-659 extent:6 https://doi.org/10.1016/j.talanta.2016.10.043 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 162 2017 1 0101 654-659 6 045F 540 |
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10.1016/j.talanta.2016.10.043 doi GBVA2017001000010.pica (DE-627)ELV01474080X (ELSEVIER)S0039-9140(16)30793-7 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Wróbel, Paweł M. verfasserin aut Combined micro-XRF and TXRF methodology for quantitative elemental imaging of tissue samples 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Spleen Elsevier Liver Elsevier Micro-XRF Elsevier Elemental imaging Elsevier Matrix effects Elsevier Kidney Elsevier TXRF Elsevier Bała, Sławomir oth Czyzycki, Mateusz oth Golasik, Magdalena oth Librowski, Tadeusz oth Ostachowicz, Beata oth Piekoszewski, Wojciech oth Surówka, Artur oth Lankosz, Marek oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:162 year:2017 day:1 month:01 pages:654-659 extent:6 https://doi.org/10.1016/j.talanta.2016.10.043 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 162 2017 1 0101 654-659 6 045F 540 |
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10.1016/j.talanta.2016.10.043 doi GBVA2017001000010.pica (DE-627)ELV01474080X (ELSEVIER)S0039-9140(16)30793-7 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Wróbel, Paweł M. verfasserin aut Combined micro-XRF and TXRF methodology for quantitative elemental imaging of tissue samples 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Spleen Elsevier Liver Elsevier Micro-XRF Elsevier Elemental imaging Elsevier Matrix effects Elsevier Kidney Elsevier TXRF Elsevier Bała, Sławomir oth Czyzycki, Mateusz oth Golasik, Magdalena oth Librowski, Tadeusz oth Ostachowicz, Beata oth Piekoszewski, Wojciech oth Surówka, Artur oth Lankosz, Marek oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:162 year:2017 day:1 month:01 pages:654-659 extent:6 https://doi.org/10.1016/j.talanta.2016.10.043 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 162 2017 1 0101 654-659 6 045F 540 |
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10.1016/j.talanta.2016.10.043 doi GBVA2017001000010.pica (DE-627)ELV01474080X (ELSEVIER)S0039-9140(16)30793-7 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Wróbel, Paweł M. verfasserin aut Combined micro-XRF and TXRF methodology for quantitative elemental imaging of tissue samples 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Spleen Elsevier Liver Elsevier Micro-XRF Elsevier Elemental imaging Elsevier Matrix effects Elsevier Kidney Elsevier TXRF Elsevier Bała, Sławomir oth Czyzycki, Mateusz oth Golasik, Magdalena oth Librowski, Tadeusz oth Ostachowicz, Beata oth Piekoszewski, Wojciech oth Surówka, Artur oth Lankosz, Marek oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:162 year:2017 day:1 month:01 pages:654-659 extent:6 https://doi.org/10.1016/j.talanta.2016.10.043 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 162 2017 1 0101 654-659 6 045F 540 |
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10.1016/j.talanta.2016.10.043 doi GBVA2017001000010.pica (DE-627)ELV01474080X (ELSEVIER)S0039-9140(16)30793-7 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Wróbel, Paweł M. verfasserin aut Combined micro-XRF and TXRF methodology for quantitative elemental imaging of tissue samples 2017transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. Spleen Elsevier Liver Elsevier Micro-XRF Elsevier Elemental imaging Elsevier Matrix effects Elsevier Kidney Elsevier TXRF Elsevier Bała, Sławomir oth Czyzycki, Mateusz oth Golasik, Magdalena oth Librowski, Tadeusz oth Ostachowicz, Beata oth Piekoszewski, Wojciech oth Surówka, Artur oth Lankosz, Marek oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:162 year:2017 day:1 month:01 pages:654-659 extent:6 https://doi.org/10.1016/j.talanta.2016.10.043 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 162 2017 1 0101 654-659 6 045F 540 |
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Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:162 year:2017 day:1 month:01 pages:654-659 extent:6 |
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Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:162 year:2017 day:1 month:01 pages:654-659 extent:6 |
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Wróbel, Paweł M. |
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Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |
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combined micro-xrf and txrf methodology for quantitative elemental imaging of tissue samples |
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Combined micro-XRF and TXRF methodology for quantitative elemental imaging of tissue samples |
| abstract |
Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. |
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Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. |
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Local differences in structural properties of biological specimens pose a major limitation to quantitative X-ray fluorescence imaging. This is because both the various tissue compartments of different density and variation in the sample thickness upon frequently used freeze-drying come up with the different values of the sample mass per unit area to be taken into account. Even though several solutions to tackle this problem based on the home-made standards for quantification in terms of thickness- and density-independent elemental mass fractions have been proposed, this issue is not addressed enough due to the samples’ heterogeneity. In our recent study, we propose a calculation scheme based on combined external-standard micro X-ray fluorescence (micro-XRF) imaging and internal-standard total reflection X-ray fluorescence (TXRF) analysis to determine the corrected elemental mass fraction distributions in commonly analysed rat tissues: kidney, liver and spleen. The results of TXRF analysis of digested large tissue sections together with the mean values of elemental masses per unit area obtained with micro-XRF were employed to determine the average masses per unit area of the samples. The correction for variation of the tissue thickness and density was done through with the use of Compton intensities. Importantly, by its versatility, our novel approach can be used to produce elemental contrast in a variety of biological specimens where local variations in either the sample density or thickness are no longer the issue. |
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Combined micro-XRF and TXRF methodology for quantitative elemental imaging of tissue samples |
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