Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor
Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET)...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chen, Hongyu [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
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| Schlagwörter: |
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| Umfang: |
8 |
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| Übergeordnetes Werk: |
Enthalten in: Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications - Mohamed, S.H. ELSEVIER, 2019, the international journal of pure and applied analytical chemistry, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:174 ; year:2017 ; day:1 ; month:11 ; pages:148-155 ; extent:8 |
| Links: |
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| DOI / URN: |
10.1016/j.talanta.2017.06.006 |
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| 245 | 1 | 0 | |a Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor |
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| 520 | |a Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. | ||
| 520 | |a Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. | ||
| 650 | 7 | |a Trypsin |2 Elsevier | |
| 650 | 7 | |a Protamine |2 Elsevier | |
| 650 | 7 | |a Fluorescence resonance energy transfer |2 Elsevier | |
| 650 | 7 | |a Silver triangular nanoplates |2 Elsevier | |
| 650 | 7 | |a Upconversion nanoparticles |2 Elsevier | |
| 700 | 1 | |a Fang, Aijin |4 oth | |
| 700 | 1 | |a Zhang, Youyu |4 oth | |
| 700 | 1 | |a Yao, Shouzhuo |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Mohamed, S.H. ELSEVIER |t Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |d 2019 |d the international journal of pure and applied analytical chemistry |g Amsterdam [u.a.] |w (DE-627)ELV003060667 |
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10.1016/j.talanta.2017.06.006 doi GBV00000000000271A.pica (DE-627)ELV014741350 (ELSEVIER)S0039-9140(17)30637-9 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Chen, Hongyu verfasserin aut Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor 2017transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Trypsin Elsevier Protamine Elsevier Fluorescence resonance energy transfer Elsevier Silver triangular nanoplates Elsevier Upconversion nanoparticles Elsevier Fang, Aijin oth Zhang, Youyu oth Yao, Shouzhuo oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:174 year:2017 day:1 month:11 pages:148-155 extent:8 https://doi.org/10.1016/j.talanta.2017.06.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 174 2017 1 1101 148-155 8 045F 540 |
| spelling |
10.1016/j.talanta.2017.06.006 doi GBV00000000000271A.pica (DE-627)ELV014741350 (ELSEVIER)S0039-9140(17)30637-9 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Chen, Hongyu verfasserin aut Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor 2017transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Trypsin Elsevier Protamine Elsevier Fluorescence resonance energy transfer Elsevier Silver triangular nanoplates Elsevier Upconversion nanoparticles Elsevier Fang, Aijin oth Zhang, Youyu oth Yao, Shouzhuo oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:174 year:2017 day:1 month:11 pages:148-155 extent:8 https://doi.org/10.1016/j.talanta.2017.06.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 174 2017 1 1101 148-155 8 045F 540 |
| allfields_unstemmed |
10.1016/j.talanta.2017.06.006 doi GBV00000000000271A.pica (DE-627)ELV014741350 (ELSEVIER)S0039-9140(17)30637-9 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Chen, Hongyu verfasserin aut Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor 2017transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Trypsin Elsevier Protamine Elsevier Fluorescence resonance energy transfer Elsevier Silver triangular nanoplates Elsevier Upconversion nanoparticles Elsevier Fang, Aijin oth Zhang, Youyu oth Yao, Shouzhuo oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:174 year:2017 day:1 month:11 pages:148-155 extent:8 https://doi.org/10.1016/j.talanta.2017.06.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 174 2017 1 1101 148-155 8 045F 540 |
| allfieldsGer |
10.1016/j.talanta.2017.06.006 doi GBV00000000000271A.pica (DE-627)ELV014741350 (ELSEVIER)S0039-9140(17)30637-9 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Chen, Hongyu verfasserin aut Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor 2017transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Trypsin Elsevier Protamine Elsevier Fluorescence resonance energy transfer Elsevier Silver triangular nanoplates Elsevier Upconversion nanoparticles Elsevier Fang, Aijin oth Zhang, Youyu oth Yao, Shouzhuo oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:174 year:2017 day:1 month:11 pages:148-155 extent:8 https://doi.org/10.1016/j.talanta.2017.06.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 174 2017 1 1101 148-155 8 045F 540 |
| allfieldsSound |
10.1016/j.talanta.2017.06.006 doi GBV00000000000271A.pica (DE-627)ELV014741350 (ELSEVIER)S0039-9140(17)30637-9 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Chen, Hongyu verfasserin aut Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor 2017transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. Trypsin Elsevier Protamine Elsevier Fluorescence resonance energy transfer Elsevier Silver triangular nanoplates Elsevier Upconversion nanoparticles Elsevier Fang, Aijin oth Zhang, Youyu oth Yao, Shouzhuo oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:174 year:2017 day:1 month:11 pages:148-155 extent:8 https://doi.org/10.1016/j.talanta.2017.06.006 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 174 2017 1 1101 148-155 8 045F 540 |
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Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:174 year:2017 day:1 month:11 pages:148-155 extent:8 |
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Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:174 year:2017 day:1 month:11 pages:148-155 extent:8 |
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Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |
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Chen, Hongyu ddc 540 ddc 530 bkl 53.56 Elsevier Trypsin Elsevier Protamine Elsevier Fluorescence resonance energy transfer Elsevier Silver triangular nanoplates Elsevier Upconversion nanoparticles Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor |
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Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |
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silver triangular nanoplates as an high efficiently fret donor-acceptor of upconversion nanoparticles for ultrasensitive “turn on-off” protamine and trypsin sensor |
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Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor |
| abstract |
Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. |
| abstractGer |
Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. |
| abstract_unstemmed |
Silver triangular nanoplates (STNPs) as a high efficient fluorescence quenching reagent of upconversion nanoparticles (UCNPs) was used to constract a novel label-free fluorescence nanosensor for ultrasensitive detection of protamine and trypsin based on fluorescence resonance energy transfer (FRET) between STNPs and UCNPs. In this assay, the negatively charged STNPs can bind with positively charged UCNPs through electrostatic interaction, and then quenched the fluorescence of UCNPs. When protamine was added to the mixture of UCNPs-STNPs, the STNPs interacted with protamine and then detached from the surface of UCNPs and aggregated, which result in the recovery of the fluorescence of UCNPs. Trypsin could catalyze the hydrolysis of protamine and effectively quench the fluorescence recovered by protamine. By measuring the changes of the fluorescence of UCNPs, the concentrations of protamine and trypsin were determined. Under the optimized conditions, the linear response range was obtained from 10 to 500ng/mL, 5–80ng/mL and with the low detection limit of 3.1ng/mL and 1.8ng/mL for protamine and trypsin, respectively. Meanwhile, the nanosensor shows good selectivity, sensitivity and can be successfully applied to detection of protamine and trypsin in serum samples. |
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Silver triangular nanoplates as an high efficiently FRET donor-acceptor of upconversion nanoparticles for ultrasensitive “Turn on-off” protamine and trypsin sensor |
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