A novel natural hirudin facilitated anti-clotting polylactide membrane via hydrogen bonding interaction
Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and i...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Li, Jinglong [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
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| Schlagwörter: |
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| Umfang: |
10 |
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| Übergeordnetes Werk: |
Enthalten in: Steering charge kinetics in W - Yue, Xin-Zheng ELSEVIER, 2019, the official journal of the North American Membrane Society, New York, NY [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:523 ; year:2017 ; day:1 ; month:02 ; pages:505-514 ; extent:10 |
| Links: |
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| DOI / URN: |
10.1016/j.memsci.2016.10.027 |
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| 245 | 1 | 0 | |a A novel natural hirudin facilitated anti-clotting polylactide membrane via hydrogen bonding interaction |
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| 520 | |a Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. | ||
| 520 | |a Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. | ||
| 650 | 7 | |a PLA membrane |2 Elsevier | |
| 650 | 7 | |a Hemocompatibility |2 Elsevier | |
| 650 | 7 | |a Dialysis |2 Elsevier | |
| 650 | 7 | |a Anti-clotting |2 Elsevier | |
| 650 | 7 | |a Hirudin |2 Elsevier | |
| 700 | 1 | |a Liu, Fu |4 oth | |
| 700 | 1 | |a Qin, Yan |4 oth | |
| 700 | 1 | |a He, Jidong |4 oth | |
| 700 | 1 | |a Xiong, Zhu |4 oth | |
| 700 | 1 | |a Deng, Gang |4 oth | |
| 700 | 1 | |a Li, Qiang |4 oth | |
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10.1016/j.memsci.2016.10.027 doi GBVA2017011000026.pica (DE-627)ELV015115488 (ELSEVIER)S0376-7388(16)31225-X DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Li, Jinglong verfasserin aut A novel natural hirudin facilitated anti-clotting polylactide membrane via hydrogen bonding interaction 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. PLA membrane Elsevier Hemocompatibility Elsevier Dialysis Elsevier Anti-clotting Elsevier Hirudin Elsevier Liu, Fu oth Qin, Yan oth He, Jidong oth Xiong, Zhu oth Deng, Gang oth Li, Qiang oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:523 year:2017 day:1 month:02 pages:505-514 extent:10 https://doi.org/10.1016/j.memsci.2016.10.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 523 2017 1 0201 505-514 10 045F 570 |
| spelling |
10.1016/j.memsci.2016.10.027 doi GBVA2017011000026.pica (DE-627)ELV015115488 (ELSEVIER)S0376-7388(16)31225-X DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Li, Jinglong verfasserin aut A novel natural hirudin facilitated anti-clotting polylactide membrane via hydrogen bonding interaction 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. PLA membrane Elsevier Hemocompatibility Elsevier Dialysis Elsevier Anti-clotting Elsevier Hirudin Elsevier Liu, Fu oth Qin, Yan oth He, Jidong oth Xiong, Zhu oth Deng, Gang oth Li, Qiang oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:523 year:2017 day:1 month:02 pages:505-514 extent:10 https://doi.org/10.1016/j.memsci.2016.10.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 523 2017 1 0201 505-514 10 045F 570 |
| allfields_unstemmed |
10.1016/j.memsci.2016.10.027 doi GBVA2017011000026.pica (DE-627)ELV015115488 (ELSEVIER)S0376-7388(16)31225-X DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Li, Jinglong verfasserin aut A novel natural hirudin facilitated anti-clotting polylactide membrane via hydrogen bonding interaction 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. PLA membrane Elsevier Hemocompatibility Elsevier Dialysis Elsevier Anti-clotting Elsevier Hirudin Elsevier Liu, Fu oth Qin, Yan oth He, Jidong oth Xiong, Zhu oth Deng, Gang oth Li, Qiang oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:523 year:2017 day:1 month:02 pages:505-514 extent:10 https://doi.org/10.1016/j.memsci.2016.10.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 523 2017 1 0201 505-514 10 045F 570 |
| allfieldsGer |
10.1016/j.memsci.2016.10.027 doi GBVA2017011000026.pica (DE-627)ELV015115488 (ELSEVIER)S0376-7388(16)31225-X DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Li, Jinglong verfasserin aut A novel natural hirudin facilitated anti-clotting polylactide membrane via hydrogen bonding interaction 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. PLA membrane Elsevier Hemocompatibility Elsevier Dialysis Elsevier Anti-clotting Elsevier Hirudin Elsevier Liu, Fu oth Qin, Yan oth He, Jidong oth Xiong, Zhu oth Deng, Gang oth Li, Qiang oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:523 year:2017 day:1 month:02 pages:505-514 extent:10 https://doi.org/10.1016/j.memsci.2016.10.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 523 2017 1 0201 505-514 10 045F 570 |
| allfieldsSound |
10.1016/j.memsci.2016.10.027 doi GBVA2017011000026.pica (DE-627)ELV015115488 (ELSEVIER)S0376-7388(16)31225-X DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Li, Jinglong verfasserin aut A novel natural hirudin facilitated anti-clotting polylactide membrane via hydrogen bonding interaction 2017transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. PLA membrane Elsevier Hemocompatibility Elsevier Dialysis Elsevier Anti-clotting Elsevier Hirudin Elsevier Liu, Fu oth Qin, Yan oth He, Jidong oth Xiong, Zhu oth Deng, Gang oth Li, Qiang oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:523 year:2017 day:1 month:02 pages:505-514 extent:10 https://doi.org/10.1016/j.memsci.2016.10.027 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 523 2017 1 0201 505-514 10 045F 570 |
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a novel natural hirudin facilitated anti-clotting polylactide membrane via hydrogen bonding interaction |
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A novel natural hirudin facilitated anti-clotting polylactide membrane via hydrogen bonding interaction |
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Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. |
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Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. |
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Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively. |
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The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Different from intensively used anticoagulant heparin, hirudin as a representative of natural bio-molecular inhibitors can form specific hirudin-thrombin complex and function as a serine proteinase to efficiently prevent blood coagulation with less bleeding adverse effect, no allergic reaction and immune of non-toxic reaction. We aim to develop a novel anticoagulant polylactide (PLA) membrane via immobilizing natural hirudin through the hydrogen bonding interaction. High amount of PVP (17.4wt%) was first immobilized on the membrane surface through the interfacial crosslinking of P(VP-VTES). The abundant carbonyl groups with high inter-association constant (K=6000) on the membrane surface dominated the hydrogen bonding interaction with the carboxyl groups of hirudin . The anti-clotting activity of PLA membrane increased with the active hirudin concentration. XPS, ATR-FTIR was conducted to confirm the chemistry evolution of PLA membranes immobilized by PVP and hirudin respectively. The intermediate PVP spacer promoted the hydrophilicity and effectively inhibited the platelet adhesion. While the enhanced hemocompatibility was distinctly revealed by the blood concretion four items (APTT, PT, TT and FIB) mainly thanks to the surface immobilization of hirudin. The anti-clotting stability of PLA membrane was evaluated with varied incubation time. The complement activation was also measured in terms of C3a and C5a. The anti-clotting PLA membrane showed excellent dialysis performances in terms of cleaning urea, creatinine, lysozyme while preserving BSA respectively.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">PLA membrane</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Hemocompatibility</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Dialysis</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Anti-clotting</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Hirudin</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Fu</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Qin, Yan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">He, Jidong</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xiong, Zhu</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Deng, Gang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Qiang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Yue, Xin-Zheng ELSEVIER</subfield><subfield code="t">Steering charge kinetics in W</subfield><subfield code="d">2019</subfield><subfield code="d">the official journal of the North American Membrane Society</subfield><subfield code="g">New York, NY [u.a.]</subfield><subfield code="w">(DE-627)ELV002478420</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:523</subfield><subfield code="g">year:2017</subfield><subfield code="g">day:1</subfield><subfield code="g">month:02</subfield><subfield code="g">pages:505-514</subfield><subfield code="g">extent:10</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.memsci.2016.10.027</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">35.17</subfield><subfield code="j">Katalyse</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">58.50</subfield><subfield code="j">Umwelttechnik: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">43.12</subfield><subfield code="j">Umweltchemie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">523</subfield><subfield code="j">2017</subfield><subfield code="b">1</subfield><subfield code="c">0201</subfield><subfield code="h">505-514</subfield><subfield code="g">10</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">570</subfield></datafield></record></collection>
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