Enhanced peroxidase-like activity of MoS2/graphene oxide hybrid with light irradiation for glucose detection
Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-...
Ausführliche Beschreibung
Autor*in: |
Peng, Jian [verfasserIn] |
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Format: |
E-Artikel |
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Sprache: |
Englisch |
Erschienen: |
2017transfer abstract |
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Umfang: |
7 |
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Übergeordnetes Werk: |
Enthalten in: Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines - Costanigro, Marco ELSEVIER, 2019, the principal international journal devoted to research, design development and application of biosensors and bioelectronics, Amsterdam [u.a.] |
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Übergeordnetes Werk: |
volume:89 ; year:2017 ; day:15 ; month:03 ; pages:652-658 ; extent:7 |
Links: |
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DOI / URN: |
10.1016/j.bios.2015.12.034 |
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Katalog-ID: |
ELV015253015 |
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520 | |a Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. | ||
520 | |a Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. | ||
650 | 7 | |a Glucose detection |2 Elsevier | |
650 | 7 | |a Synergistic interaction |2 Elsevier | |
650 | 7 | |a Peroxidase-like |2 Elsevier | |
650 | 7 | |a Light |2 Elsevier | |
650 | 7 | |a MoS2 |2 Elsevier | |
650 | 7 | |a Graphene oxide |2 Elsevier | |
700 | 1 | |a Weng, Jian |4 oth | |
773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Costanigro, Marco ELSEVIER |t Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines |d 2019 |d the principal international journal devoted to research, design development and application of biosensors and bioelectronics |g Amsterdam [u.a.] |w (DE-627)ELV001931067 |
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10.1016/j.bios.2015.12.034 doi GBV00000000000180A.pica (DE-627)ELV015253015 (ELSEVIER)S0956-5663(15)30688-6 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Peng, Jian verfasserin aut Enhanced peroxidase-like activity of MoS2/graphene oxide hybrid with light irradiation for glucose detection 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Glucose detection Elsevier Synergistic interaction Elsevier Peroxidase-like Elsevier Light Elsevier MoS2 Elsevier Graphene oxide Elsevier Weng, Jian oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:89 year:2017 day:15 month:03 pages:652-658 extent:7 https://doi.org/10.1016/j.bios.2015.12.034 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 89 2017 15 0315 652-658 7 045F 570 |
spelling |
10.1016/j.bios.2015.12.034 doi GBV00000000000180A.pica (DE-627)ELV015253015 (ELSEVIER)S0956-5663(15)30688-6 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Peng, Jian verfasserin aut Enhanced peroxidase-like activity of MoS2/graphene oxide hybrid with light irradiation for glucose detection 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Glucose detection Elsevier Synergistic interaction Elsevier Peroxidase-like Elsevier Light Elsevier MoS2 Elsevier Graphene oxide Elsevier Weng, Jian oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:89 year:2017 day:15 month:03 pages:652-658 extent:7 https://doi.org/10.1016/j.bios.2015.12.034 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 89 2017 15 0315 652-658 7 045F 570 |
allfields_unstemmed |
10.1016/j.bios.2015.12.034 doi GBV00000000000180A.pica (DE-627)ELV015253015 (ELSEVIER)S0956-5663(15)30688-6 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Peng, Jian verfasserin aut Enhanced peroxidase-like activity of MoS2/graphene oxide hybrid with light irradiation for glucose detection 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Glucose detection Elsevier Synergistic interaction Elsevier Peroxidase-like Elsevier Light Elsevier MoS2 Elsevier Graphene oxide Elsevier Weng, Jian oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:89 year:2017 day:15 month:03 pages:652-658 extent:7 https://doi.org/10.1016/j.bios.2015.12.034 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 89 2017 15 0315 652-658 7 045F 570 |
allfieldsGer |
10.1016/j.bios.2015.12.034 doi GBV00000000000180A.pica (DE-627)ELV015253015 (ELSEVIER)S0956-5663(15)30688-6 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Peng, Jian verfasserin aut Enhanced peroxidase-like activity of MoS2/graphene oxide hybrid with light irradiation for glucose detection 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Glucose detection Elsevier Synergistic interaction Elsevier Peroxidase-like Elsevier Light Elsevier MoS2 Elsevier Graphene oxide Elsevier Weng, Jian oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:89 year:2017 day:15 month:03 pages:652-658 extent:7 https://doi.org/10.1016/j.bios.2015.12.034 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 89 2017 15 0315 652-658 7 045F 570 |
allfieldsSound |
10.1016/j.bios.2015.12.034 doi GBV00000000000180A.pica (DE-627)ELV015253015 (ELSEVIER)S0956-5663(15)30688-6 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 630 640 VZ 49.00 bkl Peng, Jian verfasserin aut Enhanced peroxidase-like activity of MoS2/graphene oxide hybrid with light irradiation for glucose detection 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. Glucose detection Elsevier Synergistic interaction Elsevier Peroxidase-like Elsevier Light Elsevier MoS2 Elsevier Graphene oxide Elsevier Weng, Jian oth Enthalten in Elsevier Science Costanigro, Marco ELSEVIER Vertical differentiation via multi-tier geographical indications and the consumer perception of quality: The case of Chianti wines 2019 the principal international journal devoted to research, design development and application of biosensors and bioelectronics Amsterdam [u.a.] (DE-627)ELV001931067 volume:89 year:2017 day:15 month:03 pages:652-658 extent:7 https://doi.org/10.1016/j.bios.2015.12.034 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 49.00 Hauswirtschaft: Allgemeines VZ AR 89 2017 15 0315 652-658 7 045F 570 |
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Enhanced peroxidase-like activity of MoS2/graphene oxide hybrid with light irradiation for glucose detection |
abstract |
Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. |
abstractGer |
Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. |
abstract_unstemmed |
Construction of novel enzyme-free mimetic is very important in improving the sensitivity of biosensor. Here, an intrinsic peroxidase-like activity of MoS2 and graphene oxide (MoS2/GO) hybrid is demonstrated and the hybrid is also used to detect glucose with high sensitivity. Firstly, the peroxidase-like activity of hydrid is compared with that of two components alone, the mixture of two components and horseradish peroxidase. The results show that the hydrid has highest catalytic activity and the Michaelis constant of this hybrid is 4.35 times lower and the maximal reaction velocity is 3.34 times higher than those of horseradish peroxidase, respectively. Electrochemical technologies are used to investigate the enhancing mechanism. The results show that the excellently catalytic performance could be attributed to the fast electron transfer on the surface of MoS2/GO and the synergistic interaction of two components. Secondly, the effect of visible light and near-infrared light on the peroxidase-like activity of hybrid is also investigated. The results show that the limit of detection for H2O2 can be reduced from 10nM to 2.5nM with visible light. Thirdly, the hybrid is further used to detect glucose in serum with and without light. The results show that the hybrid has high selectivity and sensitivity for glucose detection in serum and the limit of detection for glucose is reduced from 0.83μM to 65nM with visible light. Therefore, the hybrid may have a potential application in glucose detection in serum with high sensitivity and selectivity. |
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