Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study
RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of mo...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Nunes, José C. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2014transfer abstract |
|---|
| Schlagwörter: |
|---|
| Umfang: |
10 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Steering charge kinetics in W - Yue, Xin-Zheng ELSEVIER, 2019, the official journal of the North American Membrane Society, New York, NY [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:463 ; year:2014 ; day:1 ; month:08 ; pages:1-10 ; extent:10 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.memsci.2014.03.036 |
|---|
| Katalog-ID: |
ELV01754159X |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | ELV01754159X | ||
| 003 | DE-627 | ||
| 005 | 20230625122428.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180602s2014 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.memsci.2014.03.036 |2 doi | |
| 028 | 5 | 2 | |a GBVA2014012000015.pica |
| 035 | |a (DE-627)ELV01754159X | ||
| 035 | |a (ELSEVIER)S0376-7388(14)00222-1 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 570 | |
| 082 | 0 | 4 | |a 570 |q DE-600 |
| 082 | 0 | 4 | |a 540 |q VZ |
| 084 | |a 35.17 |2 bkl | ||
| 084 | |a 58.50 |2 bkl | ||
| 084 | |a 43.12 |2 bkl | ||
| 100 | 1 | |a Nunes, José C. |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study |
| 264 | 1 | |c 2014transfer abstract | |
| 300 | |a 10 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. | ||
| 520 | |a RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. | ||
| 650 | 7 | |a Ultrafiltration |2 Elsevier | |
| 650 | 7 | |a RNA |2 Elsevier | |
| 650 | 7 | |a Plasmid DNA |2 Elsevier | |
| 650 | 7 | |a Modeling |2 Elsevier | |
| 700 | 1 | |a Pessoa de Amorim, Maria Teresa |4 oth | |
| 700 | 1 | |a Escobar, Isabel C. |4 oth | |
| 700 | 1 | |a Queiroz, João A. |4 oth | |
| 700 | 1 | |a Morão, António M. |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Yue, Xin-Zheng ELSEVIER |t Steering charge kinetics in W |d 2019 |d the official journal of the North American Membrane Society |g New York, NY [u.a.] |w (DE-627)ELV002478420 |
| 773 | 1 | 8 | |g volume:463 |g year:2014 |g day:1 |g month:08 |g pages:1-10 |g extent:10 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.memsci.2014.03.036 |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a GBV_ELV | ||
| 912 | |a SYSFLAG_U | ||
| 912 | |a SSG-OLC-PHA | ||
| 936 | b | k | |a 35.17 |j Katalyse |q VZ |
| 936 | b | k | |a 58.50 |j Umwelttechnik: Allgemeines |q VZ |
| 936 | b | k | |a 43.12 |j Umweltchemie |q VZ |
| 951 | |a AR | ||
| 952 | |d 463 |j 2014 |b 1 |c 0801 |h 1-10 |g 10 | ||
| 953 | |2 045F |a 570 | ||
| author_variant |
j c n jc jcn |
|---|---|
| matchkey_str |
nunesjoscpessoadeamorimmariateresaescoba:2014----:lsidansprtobutaitainoeig |
| hierarchy_sort_str |
2014transfer abstract |
| bklnumber |
35.17 58.50 43.12 |
| publishDate |
2014 |
| allfields |
10.1016/j.memsci.2014.03.036 doi GBVA2014012000015.pica (DE-627)ELV01754159X (ELSEVIER)S0376-7388(14)00222-1 DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Nunes, José C. verfasserin aut Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling Elsevier Pessoa de Amorim, Maria Teresa oth Escobar, Isabel C. oth Queiroz, João A. oth Morão, António M. oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:463 year:2014 day:1 month:08 pages:1-10 extent:10 https://doi.org/10.1016/j.memsci.2014.03.036 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 463 2014 1 0801 1-10 10 045F 570 |
| spelling |
10.1016/j.memsci.2014.03.036 doi GBVA2014012000015.pica (DE-627)ELV01754159X (ELSEVIER)S0376-7388(14)00222-1 DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Nunes, José C. verfasserin aut Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling Elsevier Pessoa de Amorim, Maria Teresa oth Escobar, Isabel C. oth Queiroz, João A. oth Morão, António M. oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:463 year:2014 day:1 month:08 pages:1-10 extent:10 https://doi.org/10.1016/j.memsci.2014.03.036 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 463 2014 1 0801 1-10 10 045F 570 |
| allfields_unstemmed |
10.1016/j.memsci.2014.03.036 doi GBVA2014012000015.pica (DE-627)ELV01754159X (ELSEVIER)S0376-7388(14)00222-1 DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Nunes, José C. verfasserin aut Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling Elsevier Pessoa de Amorim, Maria Teresa oth Escobar, Isabel C. oth Queiroz, João A. oth Morão, António M. oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:463 year:2014 day:1 month:08 pages:1-10 extent:10 https://doi.org/10.1016/j.memsci.2014.03.036 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 463 2014 1 0801 1-10 10 045F 570 |
| allfieldsGer |
10.1016/j.memsci.2014.03.036 doi GBVA2014012000015.pica (DE-627)ELV01754159X (ELSEVIER)S0376-7388(14)00222-1 DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Nunes, José C. verfasserin aut Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling Elsevier Pessoa de Amorim, Maria Teresa oth Escobar, Isabel C. oth Queiroz, João A. oth Morão, António M. oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:463 year:2014 day:1 month:08 pages:1-10 extent:10 https://doi.org/10.1016/j.memsci.2014.03.036 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 463 2014 1 0801 1-10 10 045F 570 |
| allfieldsSound |
10.1016/j.memsci.2014.03.036 doi GBVA2014012000015.pica (DE-627)ELV01754159X (ELSEVIER)S0376-7388(14)00222-1 DE-627 ger DE-627 rakwb eng 570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Nunes, José C. verfasserin aut Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling Elsevier Pessoa de Amorim, Maria Teresa oth Escobar, Isabel C. oth Queiroz, João A. oth Morão, António M. oth Enthalten in Elsevier Yue, Xin-Zheng ELSEVIER Steering charge kinetics in W 2019 the official journal of the North American Membrane Society New York, NY [u.a.] (DE-627)ELV002478420 volume:463 year:2014 day:1 month:08 pages:1-10 extent:10 https://doi.org/10.1016/j.memsci.2014.03.036 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 35.17 Katalyse VZ 58.50 Umwelttechnik: Allgemeines VZ 43.12 Umweltchemie VZ AR 463 2014 1 0801 1-10 10 045F 570 |
| language |
English |
| source |
Enthalten in Steering charge kinetics in W New York, NY [u.a.] volume:463 year:2014 day:1 month:08 pages:1-10 extent:10 |
| sourceStr |
Enthalten in Steering charge kinetics in W New York, NY [u.a.] volume:463 year:2014 day:1 month:08 pages:1-10 extent:10 |
| format_phy_str_mv |
Article |
| bklname |
Katalyse Umwelttechnik: Allgemeines Umweltchemie |
| institution |
findex.gbv.de |
| topic_facet |
Ultrafiltration RNA Plasmid DNA Modeling |
| dewey-raw |
570 |
| isfreeaccess_bool |
false |
| container_title |
Steering charge kinetics in W |
| authorswithroles_txt_mv |
Nunes, José C. @@aut@@ Pessoa de Amorim, Maria Teresa @@oth@@ Escobar, Isabel C. @@oth@@ Queiroz, João A. @@oth@@ Morão, António M. @@oth@@ |
| publishDateDaySort_date |
2014-01-01T00:00:00Z |
| hierarchy_top_id |
ELV002478420 |
| dewey-sort |
3570 |
| id |
ELV01754159X |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV01754159X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230625122428.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180602s2014 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.memsci.2014.03.036</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBVA2014012000015.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV01754159X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0376-7388(14)00222-1</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">35.17</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">58.50</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">43.12</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Nunes, José C.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">10</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Ultrafiltration</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">RNA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Plasmid DNA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Modeling</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pessoa de Amorim, Maria Teresa</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Escobar, Isabel C.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Queiroz, João A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Morão, António M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Yue, Xin-Zheng ELSEVIER</subfield><subfield code="t">Steering charge kinetics in W</subfield><subfield code="d">2019</subfield><subfield code="d">the official journal of the North American Membrane Society</subfield><subfield code="g">New York, NY [u.a.]</subfield><subfield code="w">(DE-627)ELV002478420</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:463</subfield><subfield code="g">year:2014</subfield><subfield code="g">day:1</subfield><subfield code="g">month:08</subfield><subfield code="g">pages:1-10</subfield><subfield code="g">extent:10</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.memsci.2014.03.036</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">35.17</subfield><subfield code="j">Katalyse</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">58.50</subfield><subfield code="j">Umwelttechnik: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">43.12</subfield><subfield code="j">Umweltchemie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">463</subfield><subfield code="j">2014</subfield><subfield code="b">1</subfield><subfield code="c">0801</subfield><subfield code="h">1-10</subfield><subfield code="g">10</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">570</subfield></datafield></record></collection>
|
| author |
Nunes, José C. |
| spellingShingle |
Nunes, José C. ddc 570 ddc 540 bkl 35.17 bkl 58.50 bkl 43.12 Elsevier Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study |
| authorStr |
Nunes, José C. |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)ELV002478420 |
| format |
electronic Article |
| dewey-ones |
570 - Life sciences; biology 540 - Chemistry & allied sciences |
| delete_txt_mv |
keep |
| author_role |
aut |
| collection |
elsevier |
| remote_str |
true |
| illustrated |
Not Illustrated |
| topic_title |
570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling Elsevier |
| topic |
ddc 570 ddc 540 bkl 35.17 bkl 58.50 bkl 43.12 Elsevier Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling |
| topic_unstemmed |
ddc 570 ddc 540 bkl 35.17 bkl 58.50 bkl 43.12 Elsevier Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling |
| topic_browse |
ddc 570 ddc 540 bkl 35.17 bkl 58.50 bkl 43.12 Elsevier Ultrafiltration Elsevier RNA Elsevier Plasmid DNA Elsevier Modeling |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
d a m t p damt damtp i c e ic ice j a q ja jaq a m m am amm |
| hierarchy_parent_title |
Steering charge kinetics in W |
| hierarchy_parent_id |
ELV002478420 |
| dewey-tens |
570 - Life sciences; biology 540 - Chemistry |
| hierarchy_top_title |
Steering charge kinetics in W |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)ELV002478420 |
| title |
Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study |
| ctrlnum |
(DE-627)ELV01754159X (ELSEVIER)S0376-7388(14)00222-1 |
| title_full |
Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study |
| author_sort |
Nunes, José C. |
| journal |
Steering charge kinetics in W |
| journalStr |
Steering charge kinetics in W |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
500 - Science |
| recordtype |
marc |
| publishDateSort |
2014 |
| contenttype_str_mv |
zzz |
| container_start_page |
1 |
| author_browse |
Nunes, José C. |
| container_volume |
463 |
| physical |
10 |
| class |
570 570 DE-600 540 VZ 35.17 bkl 58.50 bkl 43.12 bkl |
| format_se |
Elektronische Aufsätze |
| author-letter |
Nunes, José C. |
| doi_str_mv |
10.1016/j.memsci.2014.03.036 |
| dewey-full |
570 540 |
| title_sort |
plasmid dna/rna separation by ultrafiltration: modeling and application study |
| title_auth |
Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study |
| abstract |
RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. |
| abstractGer |
RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. |
| abstract_unstemmed |
RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification. |
| collection_details |
GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA |
| title_short |
Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study |
| url |
https://doi.org/10.1016/j.memsci.2014.03.036 |
| remote_bool |
true |
| author2 |
Pessoa de Amorim, Maria Teresa Escobar, Isabel C. Queiroz, João A. Morão, António M. |
| author2Str |
Pessoa de Amorim, Maria Teresa Escobar, Isabel C. Queiroz, João A. Morão, António M. |
| ppnlink |
ELV002478420 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth |
| doi_str |
10.1016/j.memsci.2014.03.036 |
| up_date |
2024-07-06T16:35:04.459Z |
| _version_ |
1803848205688897536 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV01754159X</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230625122428.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180602s2014 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.memsci.2014.03.036</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBVA2014012000015.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV01754159X</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S0376-7388(14)00222-1</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">570</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">570</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">540</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">35.17</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">58.50</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">43.12</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Nunes, José C.</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">Plasmid DNA/RNA separation by ultrafiltration: Modeling and application study</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2014transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">10</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">RNA is one of the main soluble contaminants that needs to be separated from plasmid DNA (pDNA) during its recovery process from fermentation broths. Although significantly different in molecular size, pDNA and RNA are difficult to separate by membrane filtration due to the fact that both types of molecules are highly deformable and therefore suffer flow-induced elongation when permeating through porous membranes. The possibility of performing this separation by ultrafiltration is investigated here with the aid of a theoretical model describing the interactions between two electrically charged, flexible macromolecules that simultaneously permeate through a porous membrane. The results of the simulations, applied to pDNA and the different types of RNA present in cell lysates under typical process conditions, show that only by a careful choice of the membrane pore size and the imposed permeate flux one can achieve the required selectivity in this operation. Ultrafiltration tests using microfiltered lysates from the production of two different plasmids, pVAX1-LacZ (6050bp) and pCAMBIA-1303 (12,361bp), were carried out to check the validity of the theoretical predictions; the experimental results confirm these predictions and the idea that this technique can be used in practice for pDNA purification.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Ultrafiltration</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">RNA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Plasmid DNA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Modeling</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Pessoa de Amorim, Maria Teresa</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Escobar, Isabel C.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Queiroz, João A.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Morão, António M.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Yue, Xin-Zheng ELSEVIER</subfield><subfield code="t">Steering charge kinetics in W</subfield><subfield code="d">2019</subfield><subfield code="d">the official journal of the North American Membrane Society</subfield><subfield code="g">New York, NY [u.a.]</subfield><subfield code="w">(DE-627)ELV002478420</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:463</subfield><subfield code="g">year:2014</subfield><subfield code="g">day:1</subfield><subfield code="g">month:08</subfield><subfield code="g">pages:1-10</subfield><subfield code="g">extent:10</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.memsci.2014.03.036</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">35.17</subfield><subfield code="j">Katalyse</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">58.50</subfield><subfield code="j">Umwelttechnik: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">43.12</subfield><subfield code="j">Umweltchemie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">463</subfield><subfield code="j">2014</subfield><subfield code="b">1</subfield><subfield code="c">0801</subfield><subfield code="h">1-10</subfield><subfield code="g">10</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">570</subfield></datafield></record></collection>
|
| score |
7.4021616 |