Cellular proton dynamics in Elodea canadensis leaves induced by cadmium
Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Tariq Javed, M. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014transfer abstract |
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| Schlagwörter: |
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| Umfang: |
8 |
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| Übergeordnetes Werk: |
Enthalten in: Generalized finite difference method with irregular mesh for a class of three-dimensional variable-order time-fractional advection-diffusion equations - Wang, Zhaoyang ELSEVIER, 2021, PPB : an official journal of the Federation of European Societies of Plant Physiology, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:77 ; year:2014 ; pages:15-22 ; extent:8 |
| Links: |
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| DOI / URN: |
10.1016/j.plaphy.2014.01.009 |
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ELV017780853 |
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| 245 | 1 | 0 | |a Cellular proton dynamics in Elodea canadensis leaves induced by cadmium |
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| 520 | |a Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. | ||
| 520 | |a Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. | ||
| 650 | 7 | |a Cadmium |2 Elsevier | |
| 650 | 7 | |a Apoplastic pH |2 Elsevier | |
| 650 | 7 | |a Protoplast |2 Elsevier | |
| 650 | 7 | |a Cytosolic Ca2+ |2 Elsevier | |
| 650 | 7 | |a Cytosolic pH |2 Elsevier | |
| 650 | 7 | |a Proton dynamics |2 Elsevier | |
| 650 | 7 | |a Vacuolar pH |2 Elsevier | |
| 700 | 1 | |a Lindberg, Sylvia |4 oth | |
| 700 | 1 | |a Greger, Maria |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Wang, Zhaoyang ELSEVIER |t Generalized finite difference method with irregular mesh for a class of three-dimensional variable-order time-fractional advection-diffusion equations |d 2021 |d PPB : an official journal of the Federation of European Societies of Plant Physiology |g Amsterdam [u.a.] |w (DE-627)ELV006529712 |
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10.1016/j.plaphy.2014.01.009 doi GBVA2014016000008.pica (DE-627)ELV017780853 (ELSEVIER)S0981-9428(14)00023-0 DE-627 ger DE-627 rakwb eng 630 640 580 630 DE-600 640 DE-600 580 DE-600 690 620 VZ 50.03 bkl Tariq Javed, M. verfasserin aut Cellular proton dynamics in Elodea canadensis leaves induced by cadmium 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Cadmium Elsevier Apoplastic pH Elsevier Protoplast Elsevier Cytosolic Ca2+ Elsevier Cytosolic pH Elsevier Proton dynamics Elsevier Vacuolar pH Elsevier Lindberg, Sylvia oth Greger, Maria oth Enthalten in Elsevier Science Wang, Zhaoyang ELSEVIER Generalized finite difference method with irregular mesh for a class of three-dimensional variable-order time-fractional advection-diffusion equations 2021 PPB : an official journal of the Federation of European Societies of Plant Physiology Amsterdam [u.a.] (DE-627)ELV006529712 volume:77 year:2014 pages:15-22 extent:8 https://doi.org/10.1016/j.plaphy.2014.01.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 50.03 Methoden und Techniken der Ingenieurwissenschaften VZ AR 77 2014 15-22 8 045F 630 |
| spelling |
10.1016/j.plaphy.2014.01.009 doi GBVA2014016000008.pica (DE-627)ELV017780853 (ELSEVIER)S0981-9428(14)00023-0 DE-627 ger DE-627 rakwb eng 630 640 580 630 DE-600 640 DE-600 580 DE-600 690 620 VZ 50.03 bkl Tariq Javed, M. verfasserin aut Cellular proton dynamics in Elodea canadensis leaves induced by cadmium 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Cadmium Elsevier Apoplastic pH Elsevier Protoplast Elsevier Cytosolic Ca2+ Elsevier Cytosolic pH Elsevier Proton dynamics Elsevier Vacuolar pH Elsevier Lindberg, Sylvia oth Greger, Maria oth Enthalten in Elsevier Science Wang, Zhaoyang ELSEVIER Generalized finite difference method with irregular mesh for a class of three-dimensional variable-order time-fractional advection-diffusion equations 2021 PPB : an official journal of the Federation of European Societies of Plant Physiology Amsterdam [u.a.] (DE-627)ELV006529712 volume:77 year:2014 pages:15-22 extent:8 https://doi.org/10.1016/j.plaphy.2014.01.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 50.03 Methoden und Techniken der Ingenieurwissenschaften VZ AR 77 2014 15-22 8 045F 630 |
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10.1016/j.plaphy.2014.01.009 doi GBVA2014016000008.pica (DE-627)ELV017780853 (ELSEVIER)S0981-9428(14)00023-0 DE-627 ger DE-627 rakwb eng 630 640 580 630 DE-600 640 DE-600 580 DE-600 690 620 VZ 50.03 bkl Tariq Javed, M. verfasserin aut Cellular proton dynamics in Elodea canadensis leaves induced by cadmium 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Cadmium Elsevier Apoplastic pH Elsevier Protoplast Elsevier Cytosolic Ca2+ Elsevier Cytosolic pH Elsevier Proton dynamics Elsevier Vacuolar pH Elsevier Lindberg, Sylvia oth Greger, Maria oth Enthalten in Elsevier Science Wang, Zhaoyang ELSEVIER Generalized finite difference method with irregular mesh for a class of three-dimensional variable-order time-fractional advection-diffusion equations 2021 PPB : an official journal of the Federation of European Societies of Plant Physiology Amsterdam [u.a.] (DE-627)ELV006529712 volume:77 year:2014 pages:15-22 extent:8 https://doi.org/10.1016/j.plaphy.2014.01.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 50.03 Methoden und Techniken der Ingenieurwissenschaften VZ AR 77 2014 15-22 8 045F 630 |
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10.1016/j.plaphy.2014.01.009 doi GBVA2014016000008.pica (DE-627)ELV017780853 (ELSEVIER)S0981-9428(14)00023-0 DE-627 ger DE-627 rakwb eng 630 640 580 630 DE-600 640 DE-600 580 DE-600 690 620 VZ 50.03 bkl Tariq Javed, M. verfasserin aut Cellular proton dynamics in Elodea canadensis leaves induced by cadmium 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Cadmium Elsevier Apoplastic pH Elsevier Protoplast Elsevier Cytosolic Ca2+ Elsevier Cytosolic pH Elsevier Proton dynamics Elsevier Vacuolar pH Elsevier Lindberg, Sylvia oth Greger, Maria oth Enthalten in Elsevier Science Wang, Zhaoyang ELSEVIER Generalized finite difference method with irregular mesh for a class of three-dimensional variable-order time-fractional advection-diffusion equations 2021 PPB : an official journal of the Federation of European Societies of Plant Physiology Amsterdam [u.a.] (DE-627)ELV006529712 volume:77 year:2014 pages:15-22 extent:8 https://doi.org/10.1016/j.plaphy.2014.01.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 50.03 Methoden und Techniken der Ingenieurwissenschaften VZ AR 77 2014 15-22 8 045F 630 |
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10.1016/j.plaphy.2014.01.009 doi GBVA2014016000008.pica (DE-627)ELV017780853 (ELSEVIER)S0981-9428(14)00023-0 DE-627 ger DE-627 rakwb eng 630 640 580 630 DE-600 640 DE-600 580 DE-600 690 620 VZ 50.03 bkl Tariq Javed, M. verfasserin aut Cellular proton dynamics in Elodea canadensis leaves induced by cadmium 2014transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. Cadmium Elsevier Apoplastic pH Elsevier Protoplast Elsevier Cytosolic Ca2+ Elsevier Cytosolic pH Elsevier Proton dynamics Elsevier Vacuolar pH Elsevier Lindberg, Sylvia oth Greger, Maria oth Enthalten in Elsevier Science Wang, Zhaoyang ELSEVIER Generalized finite difference method with irregular mesh for a class of three-dimensional variable-order time-fractional advection-diffusion equations 2021 PPB : an official journal of the Federation of European Societies of Plant Physiology Amsterdam [u.a.] (DE-627)ELV006529712 volume:77 year:2014 pages:15-22 extent:8 https://doi.org/10.1016/j.plaphy.2014.01.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 50.03 Methoden und Techniken der Ingenieurwissenschaften VZ AR 77 2014 15-22 8 045F 630 |
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Enthalten in Generalized finite difference method with irregular mesh for a class of three-dimensional variable-order time-fractional advection-diffusion equations Amsterdam [u.a.] volume:77 year:2014 pages:15-22 extent:8 |
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Enthalten in Generalized finite difference method with irregular mesh for a class of three-dimensional variable-order time-fractional advection-diffusion equations Amsterdam [u.a.] volume:77 year:2014 pages:15-22 extent:8 |
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cellular proton dynamics in elodea canadensis leaves induced by cadmium |
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Cellular proton dynamics in Elodea canadensis leaves induced by cadmium |
| abstract |
Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. |
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Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. |
| abstract_unstemmed |
Our earlier investigations showed that Elodea canadensis shoots, grown in the presence of cadmium (Cd), caused basification of the surrounding medium. The present study was aimed to examine the proton dynamics of the apoplastic, cytosolic and vacuolar regions of E. canadensis leaves upon Cd exposure and to establish possible linkage between cellular pH changes and the medium basification. The changes in cytosolic calcium [Ca2+]cyt was also investigated as the [Ca2+]cyt and [pH]cyt homeostasis are closely linked. The cellular H+ and Ca2+ concentrations were monitored by fluorescence microscopy and ion-specific fluorescent dyes. Cadmium concentration of leaf-cell walls was measured after plant cultivation at different fixed levels of starting pH. The protoplasts from E. canadensis leaves were isolated by use of a newly developed enzymatic method. Upon Cd addition, both cytosolic and vacuolar pH of leaf protoplasts increased with a concomitant rise in the cytosolic Ca2+ concentration. Time course studies revealed that changes in [Ca2+]cyt and [pH]cyt followed similar dynamics. Cadmium (0.5 μM) exposure decreased the apoplastic pH by 0.85 units. The maximum cell wall bound Cd-contents were obtained in plants grown at low starting pH. It is concluded that Cd treatment causes apoplastic acidosis in E. canadensis leaves associated with enhanced Cd binding to the cell walls and, consequently, reduced Cd influx into the cytosol. |
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