Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants
A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-org...
Ausführliche Beschreibung
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| Autor*in: |
Sun, Yue [verfasserIn] |
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E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015transfer abstract |
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| Umfang: |
7 |
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| Übergeordnetes Werk: |
Enthalten in: Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications - Mohamed, S.H. ELSEVIER, 2019, the international journal of pure and applied analytical chemistry, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:142 ; year:2015 ; day:1 ; month:09 ; pages:90-96 ; extent:7 |
| Links: |
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| DOI / URN: |
10.1016/j.talanta.2015.04.008 |
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ELV018132979 |
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| 245 | 1 | 0 | |a Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants |
| 264 | 1 | |c 2015transfer abstract | |
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| 520 | |a A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. | ||
| 520 | |a A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. | ||
| 650 | 7 | |a CHE |2 Elsevier | |
| 650 | 7 | |a SAN |2 Elsevier | |
| 650 | 7 | |a Flu |2 Elsevier | |
| 650 | 7 | |a Rh123 |2 Elsevier | |
| 700 | 1 | |a Li, Yuanyuan |4 oth | |
| 700 | 1 | |a Zeng, Jiajian |4 oth | |
| 700 | 1 | |a Lu, Qixian |4 oth | |
| 700 | 1 | |a Li, Paul C.H. |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier Science |a Mohamed, S.H. ELSEVIER |t Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications |d 2019 |d the international journal of pure and applied analytical chemistry |g Amsterdam [u.a.] |w (DE-627)ELV003060667 |
| 773 | 1 | 8 | |g volume:142 |g year:2015 |g day:1 |g month:09 |g pages:90-96 |g extent:7 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.talanta.2015.04.008 |3 Volltext |
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10.1016/j.talanta.2015.04.008 doi GBVA2015001000003.pica (DE-627)ELV018132979 (ELSEVIER)S0039-9140(15)00246-5 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Sun, Yue verfasserin aut Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. CHE Elsevier SAN Elsevier Flu Elsevier Rh123 Elsevier Li, Yuanyuan oth Zeng, Jiajian oth Lu, Qixian oth Li, Paul C.H. oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:142 year:2015 day:1 month:09 pages:90-96 extent:7 https://doi.org/10.1016/j.talanta.2015.04.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 142 2015 1 0901 90-96 7 045F 540 |
| spelling |
10.1016/j.talanta.2015.04.008 doi GBVA2015001000003.pica (DE-627)ELV018132979 (ELSEVIER)S0039-9140(15)00246-5 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Sun, Yue verfasserin aut Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. CHE Elsevier SAN Elsevier Flu Elsevier Rh123 Elsevier Li, Yuanyuan oth Zeng, Jiajian oth Lu, Qixian oth Li, Paul C.H. oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:142 year:2015 day:1 month:09 pages:90-96 extent:7 https://doi.org/10.1016/j.talanta.2015.04.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 142 2015 1 0901 90-96 7 045F 540 |
| allfields_unstemmed |
10.1016/j.talanta.2015.04.008 doi GBVA2015001000003.pica (DE-627)ELV018132979 (ELSEVIER)S0039-9140(15)00246-5 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Sun, Yue verfasserin aut Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. CHE Elsevier SAN Elsevier Flu Elsevier Rh123 Elsevier Li, Yuanyuan oth Zeng, Jiajian oth Lu, Qixian oth Li, Paul C.H. oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:142 year:2015 day:1 month:09 pages:90-96 extent:7 https://doi.org/10.1016/j.talanta.2015.04.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 142 2015 1 0901 90-96 7 045F 540 |
| allfieldsGer |
10.1016/j.talanta.2015.04.008 doi GBVA2015001000003.pica (DE-627)ELV018132979 (ELSEVIER)S0039-9140(15)00246-5 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Sun, Yue verfasserin aut Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. CHE Elsevier SAN Elsevier Flu Elsevier Rh123 Elsevier Li, Yuanyuan oth Zeng, Jiajian oth Lu, Qixian oth Li, Paul C.H. oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:142 year:2015 day:1 month:09 pages:90-96 extent:7 https://doi.org/10.1016/j.talanta.2015.04.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 142 2015 1 0901 90-96 7 045F 540 |
| allfieldsSound |
10.1016/j.talanta.2015.04.008 doi GBVA2015001000003.pica (DE-627)ELV018132979 (ELSEVIER)S0039-9140(15)00246-5 DE-627 ger DE-627 rakwb eng 540 540 DE-600 530 620 VZ 53.56 bkl Sun, Yue verfasserin aut Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. CHE Elsevier SAN Elsevier Flu Elsevier Rh123 Elsevier Li, Yuanyuan oth Zeng, Jiajian oth Lu, Qixian oth Li, Paul C.H. oth Enthalten in Elsevier Science Mohamed, S.H. ELSEVIER Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications 2019 the international journal of pure and applied analytical chemistry Amsterdam [u.a.] (DE-627)ELV003060667 volume:142 year:2015 day:1 month:09 pages:90-96 extent:7 https://doi.org/10.1016/j.talanta.2015.04.008 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 53.56 Halbleitertechnologie VZ AR 142 2015 1 0901 90-96 7 045F 540 |
| language |
English |
| source |
Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:142 year:2015 day:1 month:09 pages:90-96 extent:7 |
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Enthalten in Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications Amsterdam [u.a.] volume:142 year:2015 day:1 month:09 pages:90-96 extent:7 |
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Microchip electrophoretic separation and fluorescence detection of chelerythrine and sanguinarine in medicinal plants |
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A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. |
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A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. |
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A new method has been developed for separation of chelerythrine and sanguinarine in medicinal plants used in traditional Chinese medicine (TCM). The separation is achieved by microchip electrophoresis (CE) using laser-induced fluorescence detection. The CE separation is achieved by using a hydro-organic medium as the electrolyte buffer. The experimental results are consistent with the prediction by theory in terms of resolution and migration speed because of the low Joule heat generated in microchip CE. In addition, formamide was found to have a potential for separation of molecules with similar chemical structures. Based on these findings, a run buffer containing 50% formamide was used to separate chelerythrine (CHE) and sanguinarine (SAN). The influencing factors, such as solvent of run buffer, pH of buffer, separation distance, and separation voltage, were optimized. Baseline separation of chelerythrine and sanguinarine was achieved within 120s under an electrical voltage of 1.8kV. Good linearity was observed in the concentration range of 0.15–550μgmL−1 (r=0.9993) for CHE and in the range of 0.3–600μgmL−1 (r=0.9998) for SAN. A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively. |
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A low limit of detection (LOD) was achieved because of the high sensitivity achieved by laser-induced fluorescence detection (i.e. 5.0ngmL−1 and 2.0ngmL−1 for CHE and SAN, respectively). The contents of CHE are found to be 641.8±7.5 and 134.0±2.3mg/kg in extracts of Macleaya cordata and Chelidonium majus, respectively, with good recovery of above 99%. The corresponding values for SAN found in these Chinese herbal extracts are 681.8±7.9mg/kg and 890.5±8.9mg/kg, respectively.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">CHE</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">SAN</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Flu</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Rh123</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Yuanyuan</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zeng, Jiajian</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Lu, Qixian</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Paul C.H.</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier Science</subfield><subfield code="a">Mohamed, S.H. ELSEVIER</subfield><subfield code="t">Optical, water splitting and wettability of titanium nitride/titanium oxynitride bilayer films for hydrogen generation and solar cells applications</subfield><subfield code="d">2019</subfield><subfield code="d">the international journal of pure and applied analytical chemistry</subfield><subfield code="g">Amsterdam [u.a.]</subfield><subfield code="w">(DE-627)ELV003060667</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:142</subfield><subfield code="g">year:2015</subfield><subfield code="g">day:1</subfield><subfield code="g">month:09</subfield><subfield code="g">pages:90-96</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.talanta.2015.04.008</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">53.56</subfield><subfield code="j">Halbleitertechnologie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">142</subfield><subfield code="j">2015</subfield><subfield code="b">1</subfield><subfield code="c">0901</subfield><subfield code="h">90-96</subfield><subfield code="g">7</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">540</subfield></datafield></record></collection>
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