Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose
GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide s...
Ausführliche Beschreibung
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| Autor*in: |
Meng, Xiangfeng [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015transfer abstract |
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| Schlagwörter: |
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| Umfang: |
8 |
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| Übergeordnetes Werk: |
Enthalten in: An analytical model of apparent viscosity in bleeding process - Wang, Xiaochen ELSEVIER, 2021, an international journal, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:414 ; year:2015 ; day:23 ; month:09 ; pages:85-92 ; extent:8 |
| Links: |
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| DOI / URN: |
10.1016/j.carres.2015.07.011 |
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| 520 | |a GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. | ||
| 520 | |a GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. | ||
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10.1016/j.carres.2015.07.011 doi GBVA2015020000002.pica (DE-627)ELV018834817 (ELSEVIER)S0008-6215(15)00208-6 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 56.45 bkl Meng, Xiangfeng verfasserin aut Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose 2015transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. Glucansucrase Elsevier Polysaccharides Elsevier Reuteran Elsevier Lactobacillus reuteri Elsevier GTFA Elsevier Oligosaccharides Elsevier Dobruchowska, Justyna M. oth Gerwig, Gerrit J. oth Kamerling, Johannis P. oth Dijkhuizen, Lubbert oth Enthalten in Elsevier Science Wang, Xiaochen ELSEVIER An analytical model of apparent viscosity in bleeding process 2021 an international journal Amsterdam [u.a.] (DE-627)ELV006581838 volume:414 year:2015 day:23 month:09 pages:85-92 extent:8 https://doi.org/10.1016/j.carres.2015.07.011 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 56.45 Baustoffkunde VZ AR 414 2015 23 0923 85-92 8 045F 660 |
| spelling |
10.1016/j.carres.2015.07.011 doi GBVA2015020000002.pica (DE-627)ELV018834817 (ELSEVIER)S0008-6215(15)00208-6 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 56.45 bkl Meng, Xiangfeng verfasserin aut Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose 2015transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. Glucansucrase Elsevier Polysaccharides Elsevier Reuteran Elsevier Lactobacillus reuteri Elsevier GTFA Elsevier Oligosaccharides Elsevier Dobruchowska, Justyna M. oth Gerwig, Gerrit J. oth Kamerling, Johannis P. oth Dijkhuizen, Lubbert oth Enthalten in Elsevier Science Wang, Xiaochen ELSEVIER An analytical model of apparent viscosity in bleeding process 2021 an international journal Amsterdam [u.a.] (DE-627)ELV006581838 volume:414 year:2015 day:23 month:09 pages:85-92 extent:8 https://doi.org/10.1016/j.carres.2015.07.011 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 56.45 Baustoffkunde VZ AR 414 2015 23 0923 85-92 8 045F 660 |
| allfields_unstemmed |
10.1016/j.carres.2015.07.011 doi GBVA2015020000002.pica (DE-627)ELV018834817 (ELSEVIER)S0008-6215(15)00208-6 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 56.45 bkl Meng, Xiangfeng verfasserin aut Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose 2015transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. Glucansucrase Elsevier Polysaccharides Elsevier Reuteran Elsevier Lactobacillus reuteri Elsevier GTFA Elsevier Oligosaccharides Elsevier Dobruchowska, Justyna M. oth Gerwig, Gerrit J. oth Kamerling, Johannis P. oth Dijkhuizen, Lubbert oth Enthalten in Elsevier Science Wang, Xiaochen ELSEVIER An analytical model of apparent viscosity in bleeding process 2021 an international journal Amsterdam [u.a.] (DE-627)ELV006581838 volume:414 year:2015 day:23 month:09 pages:85-92 extent:8 https://doi.org/10.1016/j.carres.2015.07.011 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 56.45 Baustoffkunde VZ AR 414 2015 23 0923 85-92 8 045F 660 |
| allfieldsGer |
10.1016/j.carres.2015.07.011 doi GBVA2015020000002.pica (DE-627)ELV018834817 (ELSEVIER)S0008-6215(15)00208-6 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 56.45 bkl Meng, Xiangfeng verfasserin aut Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose 2015transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. Glucansucrase Elsevier Polysaccharides Elsevier Reuteran Elsevier Lactobacillus reuteri Elsevier GTFA Elsevier Oligosaccharides Elsevier Dobruchowska, Justyna M. oth Gerwig, Gerrit J. oth Kamerling, Johannis P. oth Dijkhuizen, Lubbert oth Enthalten in Elsevier Science Wang, Xiaochen ELSEVIER An analytical model of apparent viscosity in bleeding process 2021 an international journal Amsterdam [u.a.] (DE-627)ELV006581838 volume:414 year:2015 day:23 month:09 pages:85-92 extent:8 https://doi.org/10.1016/j.carres.2015.07.011 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 56.45 Baustoffkunde VZ AR 414 2015 23 0923 85-92 8 045F 660 |
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10.1016/j.carres.2015.07.011 doi GBVA2015020000002.pica (DE-627)ELV018834817 (ELSEVIER)S0008-6215(15)00208-6 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 56.45 bkl Meng, Xiangfeng verfasserin aut Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose 2015transfer abstract 8 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. Glucansucrase Elsevier Polysaccharides Elsevier Reuteran Elsevier Lactobacillus reuteri Elsevier GTFA Elsevier Oligosaccharides Elsevier Dobruchowska, Justyna M. oth Gerwig, Gerrit J. oth Kamerling, Johannis P. oth Dijkhuizen, Lubbert oth Enthalten in Elsevier Science Wang, Xiaochen ELSEVIER An analytical model of apparent viscosity in bleeding process 2021 an international journal Amsterdam [u.a.] (DE-627)ELV006581838 volume:414 year:2015 day:23 month:09 pages:85-92 extent:8 https://doi.org/10.1016/j.carres.2015.07.011 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 56.45 Baustoffkunde VZ AR 414 2015 23 0923 85-92 8 045F 660 |
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Enthalten in An analytical model of apparent viscosity in bleeding process Amsterdam [u.a.] volume:414 year:2015 day:23 month:09 pages:85-92 extent:8 |
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An analytical model of apparent viscosity in bleeding process |
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Synthesis of oligo- and polysaccharides by Lactobacillus reuteri 121 reuteransucrase at high concentrations of sucrose |
| abstract |
GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. |
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GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. |
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GTFA, a glucansucrase enzyme of the probiotic bacterium Lactobacillus reuteri 121, is capable of synthesizing an α-glucan polysaccharide with (1→4) and (1→6) linkages from sucrose. With respect to its biosynthesis, the present study has shown that the ratio of oligosaccharide versus polysaccharide synthesized was directly proportional to the concentration of sucrose. It appears that the size distribution of products is kinetically controlled, but the linkage distribution in the polysaccharide material is not changed. At high sucrose concentrations the sucrose isomers leucrose and trehalulose were synthesized, using the accumulated fructose as acceptor, together with 4′- and 6′-α-d-glucosyl-leucrose and 6′-α-d-glucosyl-trehalulose. The finding of an additional branched hexasaccharide demonstrates that the enzyme is able to introduce branch-points already in relatively short oligosaccharides. |
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