Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity
We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomi...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Chen, Chen [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015transfer abstract |
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| Schlagwörter: |
Dynamic microwave-assisted extraction |
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| Umfang: |
7 |
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| Übergeordnetes Werk: |
Enthalten in: Assessment of urban identity through a matrix of cultural landscapes - Ziyaee, Maryam ELSEVIER, 2017, an official journal of the Swiss Society of Food Science and Technology (SGLWT/SOSSTA) and the International Union of Food Science and Technology (IUFoST), Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:64 ; year:2015 ; number:2 ; pages:1263-1269 ; extent:7 |
| Links: |
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| DOI / URN: |
10.1016/j.lwt.2015.07.009 |
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ELV01896656X |
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| 520 | |a We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. | ||
| 520 | |a We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. | ||
| 650 | 7 | |a Dynamic microwave-assisted extraction |2 Elsevier | |
| 650 | 7 | |a Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc |2 Elsevier | |
| 650 | 7 | |a Response surface methodology |2 Elsevier | |
| 650 | 7 | |a Polysaccharide |2 Elsevier | |
| 650 | 7 | |a Biological activity |2 Elsevier | |
| 700 | 1 | |a Shao, Yun |4 oth | |
| 700 | 1 | |a Tao, Yanduo |4 oth | |
| 700 | 1 | |a Wen, Huaixiu |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Ziyaee, Maryam ELSEVIER |t Assessment of urban identity through a matrix of cultural landscapes |d 2017 |d an official journal of the Swiss Society of Food Science and Technology (SGLWT/SOSSTA) and the International Union of Food Science and Technology (IUFoST) |g Amsterdam [u.a.] |w (DE-627)ELV004078675 |
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10.1016/j.lwt.2015.07.009 doi GBVA2015022000027.pica (DE-627)ELV01896656X (ELSEVIER)S0023-6438(15)30026-8 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 74.12 bkl 74.72 bkl Chen, Chen verfasserin aut Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. Dynamic microwave-assisted extraction Elsevier Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc Elsevier Response surface methodology Elsevier Polysaccharide Elsevier Biological activity Elsevier Shao, Yun oth Tao, Yanduo oth Wen, Huaixiu oth Enthalten in Elsevier Ziyaee, Maryam ELSEVIER Assessment of urban identity through a matrix of cultural landscapes 2017 an official journal of the Swiss Society of Food Science and Technology (SGLWT/SOSSTA) and the International Union of Food Science and Technology (IUFoST) Amsterdam [u.a.] (DE-627)ELV004078675 volume:64 year:2015 number:2 pages:1263-1269 extent:7 https://doi.org/10.1016/j.lwt.2015.07.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 74.12 Stadtgeographie Siedlungsgeographie VZ 74.72 Stadtplanung kommunale Planung VZ AR 64 2015 2 1263-1269 7 045F 660 |
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10.1016/j.lwt.2015.07.009 doi GBVA2015022000027.pica (DE-627)ELV01896656X (ELSEVIER)S0023-6438(15)30026-8 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 74.12 bkl 74.72 bkl Chen, Chen verfasserin aut Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. Dynamic microwave-assisted extraction Elsevier Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc Elsevier Response surface methodology Elsevier Polysaccharide Elsevier Biological activity Elsevier Shao, Yun oth Tao, Yanduo oth Wen, Huaixiu oth Enthalten in Elsevier Ziyaee, Maryam ELSEVIER Assessment of urban identity through a matrix of cultural landscapes 2017 an official journal of the Swiss Society of Food Science and Technology (SGLWT/SOSSTA) and the International Union of Food Science and Technology (IUFoST) Amsterdam [u.a.] (DE-627)ELV004078675 volume:64 year:2015 number:2 pages:1263-1269 extent:7 https://doi.org/10.1016/j.lwt.2015.07.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 74.12 Stadtgeographie Siedlungsgeographie VZ 74.72 Stadtplanung kommunale Planung VZ AR 64 2015 2 1263-1269 7 045F 660 |
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10.1016/j.lwt.2015.07.009 doi GBVA2015022000027.pica (DE-627)ELV01896656X (ELSEVIER)S0023-6438(15)30026-8 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 74.12 bkl 74.72 bkl Chen, Chen verfasserin aut Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. Dynamic microwave-assisted extraction Elsevier Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc Elsevier Response surface methodology Elsevier Polysaccharide Elsevier Biological activity Elsevier Shao, Yun oth Tao, Yanduo oth Wen, Huaixiu oth Enthalten in Elsevier Ziyaee, Maryam ELSEVIER Assessment of urban identity through a matrix of cultural landscapes 2017 an official journal of the Swiss Society of Food Science and Technology (SGLWT/SOSSTA) and the International Union of Food Science and Technology (IUFoST) Amsterdam [u.a.] (DE-627)ELV004078675 volume:64 year:2015 number:2 pages:1263-1269 extent:7 https://doi.org/10.1016/j.lwt.2015.07.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 74.12 Stadtgeographie Siedlungsgeographie VZ 74.72 Stadtplanung kommunale Planung VZ AR 64 2015 2 1263-1269 7 045F 660 |
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10.1016/j.lwt.2015.07.009 doi GBVA2015022000027.pica (DE-627)ELV01896656X (ELSEVIER)S0023-6438(15)30026-8 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 74.12 bkl 74.72 bkl Chen, Chen verfasserin aut Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. Dynamic microwave-assisted extraction Elsevier Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc Elsevier Response surface methodology Elsevier Polysaccharide Elsevier Biological activity Elsevier Shao, Yun oth Tao, Yanduo oth Wen, Huaixiu oth Enthalten in Elsevier Ziyaee, Maryam ELSEVIER Assessment of urban identity through a matrix of cultural landscapes 2017 an official journal of the Swiss Society of Food Science and Technology (SGLWT/SOSSTA) and the International Union of Food Science and Technology (IUFoST) Amsterdam [u.a.] (DE-627)ELV004078675 volume:64 year:2015 number:2 pages:1263-1269 extent:7 https://doi.org/10.1016/j.lwt.2015.07.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 74.12 Stadtgeographie Siedlungsgeographie VZ 74.72 Stadtplanung kommunale Planung VZ AR 64 2015 2 1263-1269 7 045F 660 |
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10.1016/j.lwt.2015.07.009 doi GBVA2015022000027.pica (DE-627)ELV01896656X (ELSEVIER)S0023-6438(15)30026-8 DE-627 ger DE-627 rakwb eng 660 660 DE-600 690 VZ 74.12 bkl 74.72 bkl Chen, Chen verfasserin aut Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity 2015transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. Dynamic microwave-assisted extraction Elsevier Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc Elsevier Response surface methodology Elsevier Polysaccharide Elsevier Biological activity Elsevier Shao, Yun oth Tao, Yanduo oth Wen, Huaixiu oth Enthalten in Elsevier Ziyaee, Maryam ELSEVIER Assessment of urban identity through a matrix of cultural landscapes 2017 an official journal of the Swiss Society of Food Science and Technology (SGLWT/SOSSTA) and the International Union of Food Science and Technology (IUFoST) Amsterdam [u.a.] (DE-627)ELV004078675 volume:64 year:2015 number:2 pages:1263-1269 extent:7 https://doi.org/10.1016/j.lwt.2015.07.009 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 74.12 Stadtgeographie Siedlungsgeographie VZ 74.72 Stadtplanung kommunale Planung VZ AR 64 2015 2 1263-1269 7 045F 660 |
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Optimization of dynamic microwave-assisted extraction of Armillaria polysaccharides using RSM, and their biological activity |
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We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. |
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We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. |
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We used the Box–Behnken design to optimize polysaccharide extraction from Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. The independent factors included extraction time (X1), microwave power (X2) and water to raw material ratio (X3). The experimental values were fitted to a second-order polynomial equation using multiple regression analysis and a statistical method. Analysis of Variance results indicated that all factors including X1 – X3 had an impact on Armillaria luteo-virens (Alb. et Schw. Fr.) Sacc. polysaccharide extraction. The optimal conditions for efficient yield of polysaccharide, giving a maximum yield of 8.43%, were: X1 = 30.24 min, X2 = 600.6 W and X3 = 40 mL/g. The model was verified by modifying the optimal conditions (X1 = 30 min, X2 = 601 W and X3 = 40 mL/g) for practical application. A pilot scale test was also carried out under optimal conditions. The obtained yields 8.40 ± 0.12% and 8.34 ± 0.25% were comparable with the optimized condition, which indicated that our model is accurate. Fourier transform infrared spectroscopy characterization revealed that the extracted polysaccharide produced typical absorption peaks. Oxygen radical absorbance capacity results showed the polysaccharides had good potential as an antioxidant. Moreover, the polysaccharide showed relatively strong inhibitory activity on the growth of NCI–H446 cells. |
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