Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels — Comparing microscope and microplate methods
The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Miller, M.A. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015transfer abstract |
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| Umfang: |
5 |
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| Übergeordnetes Werk: |
Enthalten in: Purification and mass spectrometry study of Maillard reaction impurities in five acyclic nucleoside antiviral drugs - Lai, Xiaohong ELSEVIER, 2022, the international journal for marine environmental scientists, engineers, administrators, politicians and lawyers, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:101 ; year:2015 ; number:2 ; day:30 ; month:12 ; pages:903-907 ; extent:5 |
| Links: |
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| DOI / URN: |
10.1016/j.marpolbul.2015.10.072 |
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| 245 | 1 | 0 | |a Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels — Comparing microscope and microplate methods |
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| 520 | |a The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. | ||
| 520 | |a The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. | ||
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10.1016/j.marpolbul.2015.10.072 doi GBVA2015023000004.pica (DE-627)ELV018970435 (ELSEVIER)S0025-326X(15)30145-4 DE-627 ger DE-627 rakwb eng 550 333.7 550 DE-600 333.7 DE-600 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Miller, M.A. verfasserin aut Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels — Comparing microscope and microplate methods 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. Bankier, C. oth Al-Shaeri, M.A.M. oth Hartl, M.G.J. oth Enthalten in Elsevier Science Lai, Xiaohong ELSEVIER Purification and mass spectrometry study of Maillard reaction impurities in five acyclic nucleoside antiviral drugs 2022 the international journal for marine environmental scientists, engineers, administrators, politicians and lawyers Amsterdam [u.a.] (DE-627)ELV007549504 volume:101 year:2015 number:2 day:30 month:12 pages:903-907 extent:5 https://doi.org/10.1016/j.marpolbul.2015.10.072 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 101 2015 2 30 1230 903-907 5 045F 550 |
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10.1016/j.marpolbul.2015.10.072 doi GBVA2015023000004.pica (DE-627)ELV018970435 (ELSEVIER)S0025-326X(15)30145-4 DE-627 ger DE-627 rakwb eng 550 333.7 550 DE-600 333.7 DE-600 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Miller, M.A. verfasserin aut Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels — Comparing microscope and microplate methods 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. Bankier, C. oth Al-Shaeri, M.A.M. oth Hartl, M.G.J. oth Enthalten in Elsevier Science Lai, Xiaohong ELSEVIER Purification and mass spectrometry study of Maillard reaction impurities in five acyclic nucleoside antiviral drugs 2022 the international journal for marine environmental scientists, engineers, administrators, politicians and lawyers Amsterdam [u.a.] (DE-627)ELV007549504 volume:101 year:2015 number:2 day:30 month:12 pages:903-907 extent:5 https://doi.org/10.1016/j.marpolbul.2015.10.072 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 101 2015 2 30 1230 903-907 5 045F 550 |
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10.1016/j.marpolbul.2015.10.072 doi GBVA2015023000004.pica (DE-627)ELV018970435 (ELSEVIER)S0025-326X(15)30145-4 DE-627 ger DE-627 rakwb eng 550 333.7 550 DE-600 333.7 DE-600 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Miller, M.A. verfasserin aut Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels — Comparing microscope and microplate methods 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. Bankier, C. oth Al-Shaeri, M.A.M. oth Hartl, M.G.J. oth Enthalten in Elsevier Science Lai, Xiaohong ELSEVIER Purification and mass spectrometry study of Maillard reaction impurities in five acyclic nucleoside antiviral drugs 2022 the international journal for marine environmental scientists, engineers, administrators, politicians and lawyers Amsterdam [u.a.] (DE-627)ELV007549504 volume:101 year:2015 number:2 day:30 month:12 pages:903-907 extent:5 https://doi.org/10.1016/j.marpolbul.2015.10.072 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 101 2015 2 30 1230 903-907 5 045F 550 |
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10.1016/j.marpolbul.2015.10.072 doi GBVA2015023000004.pica (DE-627)ELV018970435 (ELSEVIER)S0025-326X(15)30145-4 DE-627 ger DE-627 rakwb eng 550 333.7 550 DE-600 333.7 DE-600 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Miller, M.A. verfasserin aut Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels — Comparing microscope and microplate methods 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. Bankier, C. oth Al-Shaeri, M.A.M. oth Hartl, M.G.J. oth Enthalten in Elsevier Science Lai, Xiaohong ELSEVIER Purification and mass spectrometry study of Maillard reaction impurities in five acyclic nucleoside antiviral drugs 2022 the international journal for marine environmental scientists, engineers, administrators, politicians and lawyers Amsterdam [u.a.] (DE-627)ELV007549504 volume:101 year:2015 number:2 day:30 month:12 pages:903-907 extent:5 https://doi.org/10.1016/j.marpolbul.2015.10.072 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 101 2015 2 30 1230 903-907 5 045F 550 |
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10.1016/j.marpolbul.2015.10.072 doi GBVA2015023000004.pica (DE-627)ELV018970435 (ELSEVIER)S0025-326X(15)30145-4 DE-627 ger DE-627 rakwb eng 550 333.7 550 DE-600 333.7 DE-600 610 VZ 15,3 ssgn PHARM DE-84 fid 44.40 bkl Miller, M.A. verfasserin aut Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels — Comparing microscope and microplate methods 2015transfer abstract 5 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. Bankier, C. oth Al-Shaeri, M.A.M. oth Hartl, M.G.J. oth Enthalten in Elsevier Science Lai, Xiaohong ELSEVIER Purification and mass spectrometry study of Maillard reaction impurities in five acyclic nucleoside antiviral drugs 2022 the international journal for marine environmental scientists, engineers, administrators, politicians and lawyers Amsterdam [u.a.] (DE-627)ELV007549504 volume:101 year:2015 number:2 day:30 month:12 pages:903-907 extent:5 https://doi.org/10.1016/j.marpolbul.2015.10.072 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U FID-PHARM SSG-OLC-PHA SSG-OPC-PHA 44.40 Pharmazie Pharmazeutika VZ AR 101 2015 2 30 1230 903-907 5 045F 550 |
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neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels — comparing microscope and microplate methods |
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Neutral red cytotoxicity assays for assessing in vivo carbon nanotube ecotoxicity in mussels — Comparing microscope and microplate methods |
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The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. |
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The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. |
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The purpose of the present study was to compare two neutral red retention methods, the more established but very labour-intensive microscope method (NRR) against the more recently developed microplate method (NRU). The intention was to explore whether the sample volume throughput could be increased and potential operator bias avoided. Mussels Mytilus sp. were exposed in vivo to 50, 250 and 500μgL−1 single (SWCNTs) or multi-walled carbon nanotubes (MWCNTs). Using the NRR method, SWCNTs and MWCNTs caused concentration dependent decreases in neutral red retention time. However, a concentration dependent decrease in optical density was not observed using the NRU method. We conclude that the NRU method is not sensitive enough to assess carbon nanotube ecotoxicity in vivo in environmentally relevant media, and recommend using the NRR method. |
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