Crystal defects induced by chitin and chitinolytic enzymes in the prismatic layer of Pinctada fucata
Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than ab...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Kintsu, Hiroyuki [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
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| Schlagwörter: |
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| Umfang: |
7 |
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| Übergeordnetes Werk: |
Enthalten in: Preparation and characterization of glass-ceramics via co-sintering of coal fly ash and oil shale ash-derived amorphous slag - Zhang, Zhikun ELSEVIER, 2019, BBRC, Orlando, Fla |
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| Übergeordnetes Werk: |
volume:489 ; year:2017 ; number:2 ; day:22 ; month:07 ; pages:89-95 ; extent:7 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.bbrc.2017.05.088 |
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ELV02049503X |
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| 520 | |a Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. | ||
| 520 | |a Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. | ||
| 650 | 7 | |a Chitinase |2 Elsevier | |
| 650 | 7 | |a Biomineralization |2 Elsevier | |
| 650 | 7 | |a Prismatic layer |2 Elsevier | |
| 650 | 7 | |a Chitin |2 Elsevier | |
| 700 | 1 | |a Okumura, Taiga |4 oth | |
| 700 | 1 | |a Negishi, Lumi |4 oth | |
| 700 | 1 | |a Ifuku, Shinsuke |4 oth | |
| 700 | 1 | |a Kogure, Toshihiro |4 oth | |
| 700 | 1 | |a Sakuda, Shohei |4 oth | |
| 700 | 1 | |a Suzuki, Michio |4 oth | |
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10.1016/j.bbrc.2017.05.088 doi GBV00000000000262A.pica (DE-627)ELV02049503X (ELSEVIER)S0006-291X(17)30962-2 DE-627 ger DE-627 rakwb eng 570 570 DE-600 670 VZ 51.60 bkl 58.45 bkl Kintsu, Hiroyuki verfasserin aut Crystal defects induced by chitin and chitinolytic enzymes in the prismatic layer of Pinctada fucata 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Chitinase Elsevier Biomineralization Elsevier Prismatic layer Elsevier Chitin Elsevier Okumura, Taiga oth Negishi, Lumi oth Ifuku, Shinsuke oth Kogure, Toshihiro oth Sakuda, Shohei oth Suzuki, Michio oth Enthalten in Academic Press Zhang, Zhikun ELSEVIER Preparation and characterization of glass-ceramics via co-sintering of coal fly ash and oil shale ash-derived amorphous slag 2019 BBRC Orlando, Fla (DE-627)ELV002811154 volume:489 year:2017 number:2 day:22 month:07 pages:89-95 extent:7 https://doi.org/10.1016/j.bbrc.2017.05.088 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 489 2017 2 22 0722 89-95 7 045F 570 |
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10.1016/j.bbrc.2017.05.088 doi GBV00000000000262A.pica (DE-627)ELV02049503X (ELSEVIER)S0006-291X(17)30962-2 DE-627 ger DE-627 rakwb eng 570 570 DE-600 670 VZ 51.60 bkl 58.45 bkl Kintsu, Hiroyuki verfasserin aut Crystal defects induced by chitin and chitinolytic enzymes in the prismatic layer of Pinctada fucata 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Chitinase Elsevier Biomineralization Elsevier Prismatic layer Elsevier Chitin Elsevier Okumura, Taiga oth Negishi, Lumi oth Ifuku, Shinsuke oth Kogure, Toshihiro oth Sakuda, Shohei oth Suzuki, Michio oth Enthalten in Academic Press Zhang, Zhikun ELSEVIER Preparation and characterization of glass-ceramics via co-sintering of coal fly ash and oil shale ash-derived amorphous slag 2019 BBRC Orlando, Fla (DE-627)ELV002811154 volume:489 year:2017 number:2 day:22 month:07 pages:89-95 extent:7 https://doi.org/10.1016/j.bbrc.2017.05.088 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 489 2017 2 22 0722 89-95 7 045F 570 |
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10.1016/j.bbrc.2017.05.088 doi GBV00000000000262A.pica (DE-627)ELV02049503X (ELSEVIER)S0006-291X(17)30962-2 DE-627 ger DE-627 rakwb eng 570 570 DE-600 670 VZ 51.60 bkl 58.45 bkl Kintsu, Hiroyuki verfasserin aut Crystal defects induced by chitin and chitinolytic enzymes in the prismatic layer of Pinctada fucata 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Chitinase Elsevier Biomineralization Elsevier Prismatic layer Elsevier Chitin Elsevier Okumura, Taiga oth Negishi, Lumi oth Ifuku, Shinsuke oth Kogure, Toshihiro oth Sakuda, Shohei oth Suzuki, Michio oth Enthalten in Academic Press Zhang, Zhikun ELSEVIER Preparation and characterization of glass-ceramics via co-sintering of coal fly ash and oil shale ash-derived amorphous slag 2019 BBRC Orlando, Fla (DE-627)ELV002811154 volume:489 year:2017 number:2 day:22 month:07 pages:89-95 extent:7 https://doi.org/10.1016/j.bbrc.2017.05.088 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 489 2017 2 22 0722 89-95 7 045F 570 |
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10.1016/j.bbrc.2017.05.088 doi GBV00000000000262A.pica (DE-627)ELV02049503X (ELSEVIER)S0006-291X(17)30962-2 DE-627 ger DE-627 rakwb eng 570 570 DE-600 670 VZ 51.60 bkl 58.45 bkl Kintsu, Hiroyuki verfasserin aut Crystal defects induced by chitin and chitinolytic enzymes in the prismatic layer of Pinctada fucata 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Chitinase Elsevier Biomineralization Elsevier Prismatic layer Elsevier Chitin Elsevier Okumura, Taiga oth Negishi, Lumi oth Ifuku, Shinsuke oth Kogure, Toshihiro oth Sakuda, Shohei oth Suzuki, Michio oth Enthalten in Academic Press Zhang, Zhikun ELSEVIER Preparation and characterization of glass-ceramics via co-sintering of coal fly ash and oil shale ash-derived amorphous slag 2019 BBRC Orlando, Fla (DE-627)ELV002811154 volume:489 year:2017 number:2 day:22 month:07 pages:89-95 extent:7 https://doi.org/10.1016/j.bbrc.2017.05.088 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 489 2017 2 22 0722 89-95 7 045F 570 |
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10.1016/j.bbrc.2017.05.088 doi GBV00000000000262A.pica (DE-627)ELV02049503X (ELSEVIER)S0006-291X(17)30962-2 DE-627 ger DE-627 rakwb eng 570 570 DE-600 670 VZ 51.60 bkl 58.45 bkl Kintsu, Hiroyuki verfasserin aut Crystal defects induced by chitin and chitinolytic enzymes in the prismatic layer of Pinctada fucata 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. Chitinase Elsevier Biomineralization Elsevier Prismatic layer Elsevier Chitin Elsevier Okumura, Taiga oth Negishi, Lumi oth Ifuku, Shinsuke oth Kogure, Toshihiro oth Sakuda, Shohei oth Suzuki, Michio oth Enthalten in Academic Press Zhang, Zhikun ELSEVIER Preparation and characterization of glass-ceramics via co-sintering of coal fly ash and oil shale ash-derived amorphous slag 2019 BBRC Orlando, Fla (DE-627)ELV002811154 volume:489 year:2017 number:2 day:22 month:07 pages:89-95 extent:7 https://doi.org/10.1016/j.bbrc.2017.05.088 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 489 2017 2 22 0722 89-95 7 045F 570 |
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English |
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Enthalten in Preparation and characterization of glass-ceramics via co-sintering of coal fly ash and oil shale ash-derived amorphous slag Orlando, Fla volume:489 year:2017 number:2 day:22 month:07 pages:89-95 extent:7 |
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Enthalten in Preparation and characterization of glass-ceramics via co-sintering of coal fly ash and oil shale ash-derived amorphous slag Orlando, Fla volume:489 year:2017 number:2 day:22 month:07 pages:89-95 extent:7 |
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Preparation and characterization of glass-ceramics via co-sintering of coal fly ash and oil shale ash-derived amorphous slag |
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crystal defects induced by chitin and chitinolytic enzymes in the prismatic layer of pinctada fucata |
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Crystal defects induced by chitin and chitinolytic enzymes in the prismatic layer of Pinctada fucata |
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Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. |
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Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. |
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Biomineralization, in which organisms create biogenic hard tissues, with hardness or flexibility enhanced by organic–inorganic interaction is an interesting and attractive focus for application of biomimetic functional materials. Calcites in the prismatic layer of Pinctada fucata are tougher than abiotic calcites due to small crystal defects. However, the molecular mechanism of the defect formation remains unclear. Here, chitin and two chitinolytic enzymes, chitinase and chitobiase, were identified as organic matrices related to for the formation of small crystal defects in the prismatic layer. Experiments with a chitinase inhibitor in vivo showed chitinase is necessary to form the prismatic layer. Analysis of calcite crystals, which were synthesized in a chitin hydrogel treated with chitinolytic enzymes, by electron microscopy and X-ray diffraction showed that crystal defects became larger as chitin was more degraded. These results suggest that interactions between chitin and calcium carbonate increase as chitin is thinner. |
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