Tandem determination of mitoxantrone and ribonucleic acid using mercaptosuccinic acid-capped CdTe quantum dots
Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the s...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Mohammadinejad, Arash [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
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| Schlagwörter: |
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| Umfang: |
7 |
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| Übergeordnetes Werk: |
Enthalten in: New ablation evolution behaviors in micro-hole drilling of 2.5D C - Liu, Chang ELSEVIER, 2021, New York, NY [u.a.] |
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| Übergeordnetes Werk: |
volume:190 ; year:2017 ; pages:254-260 ; extent:7 |
| Links: |
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| DOI / URN: |
10.1016/j.jlumin.2017.05.054 |
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ELV020563574 |
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| 245 | 1 | 0 | |a Tandem determination of mitoxantrone and ribonucleic acid using mercaptosuccinic acid-capped CdTe quantum dots |
| 264 | 1 | |c 2017transfer abstract | |
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| 520 | |a Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. | ||
| 520 | |a Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. | ||
| 650 | 7 | |a MTX |2 Elsevier | |
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| 700 | 1 | |a Mohajeri, Seyed Ahmad |4 oth | |
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10.1016/j.jlumin.2017.05.054 doi GBVA2017021000001.pica (DE-627)ELV020563574 (ELSEVIER)S0022-2313(16)31944-5 DE-627 ger DE-627 rakwb eng 530 530 DE-600 670 VZ 51.60 bkl 58.45 bkl Mohammadinejad, Arash verfasserin aut Tandem determination of mitoxantrone and ribonucleic acid using mercaptosuccinic acid-capped CdTe quantum dots 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. MTX Elsevier MSA-CdTe QDs Elsevier FI Elsevier RNA Elsevier Es'haghi, Zarrin oth Abnous, Khalil oth Mohajeri, Seyed Ahmad oth Enthalten in Elsevier Liu, Chang ELSEVIER New ablation evolution behaviors in micro-hole drilling of 2.5D C 2021 New York, NY [u.a.] (DE-627)ELV00662605X volume:190 year:2017 pages:254-260 extent:7 https://doi.org/10.1016/j.jlumin.2017.05.054 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 190 2017 254-260 7 045F 530 |
| spelling |
10.1016/j.jlumin.2017.05.054 doi GBVA2017021000001.pica (DE-627)ELV020563574 (ELSEVIER)S0022-2313(16)31944-5 DE-627 ger DE-627 rakwb eng 530 530 DE-600 670 VZ 51.60 bkl 58.45 bkl Mohammadinejad, Arash verfasserin aut Tandem determination of mitoxantrone and ribonucleic acid using mercaptosuccinic acid-capped CdTe quantum dots 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. MTX Elsevier MSA-CdTe QDs Elsevier FI Elsevier RNA Elsevier Es'haghi, Zarrin oth Abnous, Khalil oth Mohajeri, Seyed Ahmad oth Enthalten in Elsevier Liu, Chang ELSEVIER New ablation evolution behaviors in micro-hole drilling of 2.5D C 2021 New York, NY [u.a.] (DE-627)ELV00662605X volume:190 year:2017 pages:254-260 extent:7 https://doi.org/10.1016/j.jlumin.2017.05.054 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 190 2017 254-260 7 045F 530 |
| allfields_unstemmed |
10.1016/j.jlumin.2017.05.054 doi GBVA2017021000001.pica (DE-627)ELV020563574 (ELSEVIER)S0022-2313(16)31944-5 DE-627 ger DE-627 rakwb eng 530 530 DE-600 670 VZ 51.60 bkl 58.45 bkl Mohammadinejad, Arash verfasserin aut Tandem determination of mitoxantrone and ribonucleic acid using mercaptosuccinic acid-capped CdTe quantum dots 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. MTX Elsevier MSA-CdTe QDs Elsevier FI Elsevier RNA Elsevier Es'haghi, Zarrin oth Abnous, Khalil oth Mohajeri, Seyed Ahmad oth Enthalten in Elsevier Liu, Chang ELSEVIER New ablation evolution behaviors in micro-hole drilling of 2.5D C 2021 New York, NY [u.a.] (DE-627)ELV00662605X volume:190 year:2017 pages:254-260 extent:7 https://doi.org/10.1016/j.jlumin.2017.05.054 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 190 2017 254-260 7 045F 530 |
| allfieldsGer |
10.1016/j.jlumin.2017.05.054 doi GBVA2017021000001.pica (DE-627)ELV020563574 (ELSEVIER)S0022-2313(16)31944-5 DE-627 ger DE-627 rakwb eng 530 530 DE-600 670 VZ 51.60 bkl 58.45 bkl Mohammadinejad, Arash verfasserin aut Tandem determination of mitoxantrone and ribonucleic acid using mercaptosuccinic acid-capped CdTe quantum dots 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. MTX Elsevier MSA-CdTe QDs Elsevier FI Elsevier RNA Elsevier Es'haghi, Zarrin oth Abnous, Khalil oth Mohajeri, Seyed Ahmad oth Enthalten in Elsevier Liu, Chang ELSEVIER New ablation evolution behaviors in micro-hole drilling of 2.5D C 2021 New York, NY [u.a.] (DE-627)ELV00662605X volume:190 year:2017 pages:254-260 extent:7 https://doi.org/10.1016/j.jlumin.2017.05.054 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 190 2017 254-260 7 045F 530 |
| allfieldsSound |
10.1016/j.jlumin.2017.05.054 doi GBVA2017021000001.pica (DE-627)ELV020563574 (ELSEVIER)S0022-2313(16)31944-5 DE-627 ger DE-627 rakwb eng 530 530 DE-600 670 VZ 51.60 bkl 58.45 bkl Mohammadinejad, Arash verfasserin aut Tandem determination of mitoxantrone and ribonucleic acid using mercaptosuccinic acid-capped CdTe quantum dots 2017transfer abstract 7 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. MTX Elsevier MSA-CdTe QDs Elsevier FI Elsevier RNA Elsevier Es'haghi, Zarrin oth Abnous, Khalil oth Mohajeri, Seyed Ahmad oth Enthalten in Elsevier Liu, Chang ELSEVIER New ablation evolution behaviors in micro-hole drilling of 2.5D C 2021 New York, NY [u.a.] (DE-627)ELV00662605X volume:190 year:2017 pages:254-260 extent:7 https://doi.org/10.1016/j.jlumin.2017.05.054 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.60 Keramische Werkstoffe Hartstoffe Werkstoffkunde VZ 58.45 Gesteinshüttenkunde VZ AR 190 2017 254-260 7 045F 530 |
| language |
English |
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Enthalten in New ablation evolution behaviors in micro-hole drilling of 2.5D C New York, NY [u.a.] volume:190 year:2017 pages:254-260 extent:7 |
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Tandem determination of mitoxantrone and ribonucleic acid using mercaptosuccinic acid-capped CdTe quantum dots |
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Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. |
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Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. |
| abstract_unstemmed |
Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%. |
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The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Mercaptosuccinic acid-capped CdTe quantum dots were successfully fabricated as a simple synthesized and sensitive fluorescence sensor for tandem determination of mitoxantrone and ribonucleic acid and also monitoring their interaction. Due to the adsorption of positively-charged mitoxantrone on the surface of negatively-charged quantum dots through electrostatic interactions, the fluorescence intensity of mercaptosuccinic acid-capped CdTe quantum dots can be effectively quenched by mitoxantrone. Determination of mitoxantrone was also done through the quenching process, in the range of 0.0675–0.337µM with LOD of 0.025µM. Total ribonucleic acid was extracted by AccuZol™ reagent from liver tissue of rat. The concentration of ribonucleic acid was determined 5–6µg/µL at 260nm by spectrophotometry (NanoDrop). The 28S and 18S bands of ribosomal ribonucleic acid were obviously revealed on agarose gel electrophoresis which confirmed quality of extracted ribonucleic acid. Nucleic acid purity was monitored by 260/280nm wavelength absorbance ratio. The ratio was1.97±0.01. After addition of ribonucleic acid to mitoxantrone–quantum dots solution, mitoxantrone mainly bound to the uracil (C˭O) and adenine (C˭N) sites of ribonucleic acid. This complex which was formed between mitoxantrone and ribonucleic acid, prevented more interactions between quantum dots and anticancer drug resulted in enhancing of fluorescence intensity. Thus, the ribonucleic acid determination in the range of 0.3–2.5ng/µL was based on the abovementioned fluorescence enhancing process with detection limit of 0.1ng/µL. The proposed method was utilized for the determination of mitoxantrone and ribonucleic acid in the human serum with recovery ranged from 70% to 110% with relative standard deviations of 3.8–10%.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">MTX</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">MSA-CdTe QDs</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">FI</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">RNA</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Es'haghi, Zarrin</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Abnous, Khalil</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Mohajeri, Seyed Ahmad</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Liu, Chang ELSEVIER</subfield><subfield code="t">New ablation evolution behaviors in micro-hole drilling of 2.5D C</subfield><subfield code="d">2021</subfield><subfield code="g">New York, NY [u.a.]</subfield><subfield code="w">(DE-627)ELV00662605X</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:190</subfield><subfield code="g">year:2017</subfield><subfield code="g">pages:254-260</subfield><subfield code="g">extent:7</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.jlumin.2017.05.054</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">51.60</subfield><subfield code="j">Keramische Werkstoffe</subfield><subfield code="j">Hartstoffe</subfield><subfield code="x">Werkstoffkunde</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">58.45</subfield><subfield code="j">Gesteinshüttenkunde</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">190</subfield><subfield code="j">2017</subfield><subfield code="h">254-260</subfield><subfield code="g">7</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">530</subfield></datafield></record></collection>
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