Sample preparation methods for quantitative detection of DNA by molecular assays and marine biosensors
• DNA extraction methods affected measured qPCR target recovery. • Recovery and variability differed, sometimes by more than an order of magnitude. • SCODA did not offer significant improvement with PCR-inhibited seawater. • Aggressive lysis did appear to improve target recovery. • Reliable and affo...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Cox, Annie M. [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2013 |
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| Schlagwörter: |
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| Umfang: |
10 |
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| Übergeordnetes Werk: |
Enthalten in: Purification and mass spectrometry study of Maillard reaction impurities in five acyclic nucleoside antiviral drugs - Lai, Xiaohong ELSEVIER, 2022, the international journal for marine environmental scientists, engineers, administrators, politicians and lawyers, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:73 ; year:2013 ; number:1 ; day:15 ; month:08 ; pages:47-56 ; extent:10 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.marpolbul.2013.06.006 |
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ELV022316469 |
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| 520 | |a • DNA extraction methods affected measured qPCR target recovery. • Recovery and variability differed, sometimes by more than an order of magnitude. • SCODA did not offer significant improvement with PCR-inhibited seawater. • Aggressive lysis did appear to improve target recovery. • Reliable and affordable correction methods are needed for quantitative PCR. | ||
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Sample preparation methods for quantitative detection of DNA by molecular assays and marine biosensors |
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• DNA extraction methods affected measured qPCR target recovery. • Recovery and variability differed, sometimes by more than an order of magnitude. • SCODA did not offer significant improvement with PCR-inhibited seawater. • Aggressive lysis did appear to improve target recovery. • Reliable and affordable correction methods are needed for quantitative PCR. |
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• DNA extraction methods affected measured qPCR target recovery. • Recovery and variability differed, sometimes by more than an order of magnitude. • SCODA did not offer significant improvement with PCR-inhibited seawater. • Aggressive lysis did appear to improve target recovery. • Reliable and affordable correction methods are needed for quantitative PCR. |
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• DNA extraction methods affected measured qPCR target recovery. • Recovery and variability differed, sometimes by more than an order of magnitude. • SCODA did not offer significant improvement with PCR-inhibited seawater. • Aggressive lysis did appear to improve target recovery. • Reliable and affordable correction methods are needed for quantitative PCR. |
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