Development of a live attenuated vaccine candidate against duck Tembusu viral disease
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX...
Ausführliche Beschreibung
Autor*in: |
Li, Guoxin [verfasserIn] |
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Englisch |
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2014transfer abstract |
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10 |
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Enthalten in: Resistive switching in 2D bismuth oxyhalide nanosheets for nonvolatile memory and emulation of leaky integrate-and-fire functions - Xie, Bingyang ELSEVIER, 2022, San Diego, Calif. [u.a.] |
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volume:450 ; year:2014 ; pages:233-242 ; extent:10 |
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10.1016/j.virol.2013.12.028 |
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520 | |a Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. | ||
520 | |a Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. | ||
650 | 7 | |a Duck Tembusu virus |2 Elsevier | |
650 | 7 | |a Attenuation |2 Elsevier | |
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700 | 1 | |a Gao, Xuyuan |4 oth | |
700 | 1 | |a Xiao, Yali |4 oth | |
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700 | 1 | |a Peng, Shan |4 oth | |
700 | 1 | |a Li, Xuesong |4 oth | |
700 | 1 | |a Shi, Ying |4 oth | |
700 | 1 | |a Zhang, Yuee |4 oth | |
700 | 1 | |a Yu, Lei |4 oth | |
700 | 1 | |a Wu, Xiaogang |4 oth | |
700 | 1 | |a Yan, Pixi |4 oth | |
700 | 1 | |a Yan, Liping |4 oth | |
700 | 1 | |a Teng, Qiaoyang |4 oth | |
700 | 1 | |a Tong, Guangzhi |4 oth | |
700 | 1 | |a Li, Zejun |4 oth | |
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10.1016/j.virol.2013.12.028 doi GBVA2014001000026.pica (DE-627)ELV02237888X (ELSEVIER)S0042-6822(13)00691-0 DE-627 ger DE-627 rakwb eng 610 570 610 DE-600 570 DE-600 600 690 VZ 51.00 bkl 51.32 bkl Li, Guoxin verfasserin aut Development of a live attenuated vaccine candidate against duck Tembusu viral disease 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus Elsevier Attenuation Elsevier Flavivirus Elsevier Gao, Xuyuan oth Xiao, Yali oth Liu, Shaoqiong oth Peng, Shan oth Li, Xuesong oth Shi, Ying oth Zhang, Yuee oth Yu, Lei oth Wu, Xiaogang oth Yan, Pixi oth Yan, Liping oth Teng, Qiaoyang oth Tong, Guangzhi oth Li, Zejun oth Enthalten in Elsevier Xie, Bingyang ELSEVIER Resistive switching in 2D bismuth oxyhalide nanosheets for nonvolatile memory and emulation of leaky integrate-and-fire functions 2022 San Diego, Calif. [u.a.] (DE-627)ELV008536686 volume:450 year:2014 pages:233-242 extent:10 https://doi.org/10.1016/j.virol.2013.12.028 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.00 Werkstoffkunde: Allgemeines VZ 51.32 Werkstoffmechanik VZ AR 450 2014 233-242 10 045F 610 |
spelling |
10.1016/j.virol.2013.12.028 doi GBVA2014001000026.pica (DE-627)ELV02237888X (ELSEVIER)S0042-6822(13)00691-0 DE-627 ger DE-627 rakwb eng 610 570 610 DE-600 570 DE-600 600 690 VZ 51.00 bkl 51.32 bkl Li, Guoxin verfasserin aut Development of a live attenuated vaccine candidate against duck Tembusu viral disease 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus Elsevier Attenuation Elsevier Flavivirus Elsevier Gao, Xuyuan oth Xiao, Yali oth Liu, Shaoqiong oth Peng, Shan oth Li, Xuesong oth Shi, Ying oth Zhang, Yuee oth Yu, Lei oth Wu, Xiaogang oth Yan, Pixi oth Yan, Liping oth Teng, Qiaoyang oth Tong, Guangzhi oth Li, Zejun oth Enthalten in Elsevier Xie, Bingyang ELSEVIER Resistive switching in 2D bismuth oxyhalide nanosheets for nonvolatile memory and emulation of leaky integrate-and-fire functions 2022 San Diego, Calif. [u.a.] (DE-627)ELV008536686 volume:450 year:2014 pages:233-242 extent:10 https://doi.org/10.1016/j.virol.2013.12.028 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.00 Werkstoffkunde: Allgemeines VZ 51.32 Werkstoffmechanik VZ AR 450 2014 233-242 10 045F 610 |
allfields_unstemmed |
10.1016/j.virol.2013.12.028 doi GBVA2014001000026.pica (DE-627)ELV02237888X (ELSEVIER)S0042-6822(13)00691-0 DE-627 ger DE-627 rakwb eng 610 570 610 DE-600 570 DE-600 600 690 VZ 51.00 bkl 51.32 bkl Li, Guoxin verfasserin aut Development of a live attenuated vaccine candidate against duck Tembusu viral disease 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus Elsevier Attenuation Elsevier Flavivirus Elsevier Gao, Xuyuan oth Xiao, Yali oth Liu, Shaoqiong oth Peng, Shan oth Li, Xuesong oth Shi, Ying oth Zhang, Yuee oth Yu, Lei oth Wu, Xiaogang oth Yan, Pixi oth Yan, Liping oth Teng, Qiaoyang oth Tong, Guangzhi oth Li, Zejun oth Enthalten in Elsevier Xie, Bingyang ELSEVIER Resistive switching in 2D bismuth oxyhalide nanosheets for nonvolatile memory and emulation of leaky integrate-and-fire functions 2022 San Diego, Calif. [u.a.] (DE-627)ELV008536686 volume:450 year:2014 pages:233-242 extent:10 https://doi.org/10.1016/j.virol.2013.12.028 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.00 Werkstoffkunde: Allgemeines VZ 51.32 Werkstoffmechanik VZ AR 450 2014 233-242 10 045F 610 |
allfieldsGer |
10.1016/j.virol.2013.12.028 doi GBVA2014001000026.pica (DE-627)ELV02237888X (ELSEVIER)S0042-6822(13)00691-0 DE-627 ger DE-627 rakwb eng 610 570 610 DE-600 570 DE-600 600 690 VZ 51.00 bkl 51.32 bkl Li, Guoxin verfasserin aut Development of a live attenuated vaccine candidate against duck Tembusu viral disease 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus Elsevier Attenuation Elsevier Flavivirus Elsevier Gao, Xuyuan oth Xiao, Yali oth Liu, Shaoqiong oth Peng, Shan oth Li, Xuesong oth Shi, Ying oth Zhang, Yuee oth Yu, Lei oth Wu, Xiaogang oth Yan, Pixi oth Yan, Liping oth Teng, Qiaoyang oth Tong, Guangzhi oth Li, Zejun oth Enthalten in Elsevier Xie, Bingyang ELSEVIER Resistive switching in 2D bismuth oxyhalide nanosheets for nonvolatile memory and emulation of leaky integrate-and-fire functions 2022 San Diego, Calif. [u.a.] (DE-627)ELV008536686 volume:450 year:2014 pages:233-242 extent:10 https://doi.org/10.1016/j.virol.2013.12.028 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.00 Werkstoffkunde: Allgemeines VZ 51.32 Werkstoffmechanik VZ AR 450 2014 233-242 10 045F 610 |
allfieldsSound |
10.1016/j.virol.2013.12.028 doi GBVA2014001000026.pica (DE-627)ELV02237888X (ELSEVIER)S0042-6822(13)00691-0 DE-627 ger DE-627 rakwb eng 610 570 610 DE-600 570 DE-600 600 690 VZ 51.00 bkl 51.32 bkl Li, Guoxin verfasserin aut Development of a live attenuated vaccine candidate against duck Tembusu viral disease 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. Duck Tembusu virus Elsevier Attenuation Elsevier Flavivirus Elsevier Gao, Xuyuan oth Xiao, Yali oth Liu, Shaoqiong oth Peng, Shan oth Li, Xuesong oth Shi, Ying oth Zhang, Yuee oth Yu, Lei oth Wu, Xiaogang oth Yan, Pixi oth Yan, Liping oth Teng, Qiaoyang oth Tong, Guangzhi oth Li, Zejun oth Enthalten in Elsevier Xie, Bingyang ELSEVIER Resistive switching in 2D bismuth oxyhalide nanosheets for nonvolatile memory and emulation of leaky integrate-and-fire functions 2022 San Diego, Calif. [u.a.] (DE-627)ELV008536686 volume:450 year:2014 pages:233-242 extent:10 https://doi.org/10.1016/j.virol.2013.12.028 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U 51.00 Werkstoffkunde: Allgemeines VZ 51.32 Werkstoffmechanik VZ AR 450 2014 233-242 10 045F 610 |
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Enthalten in Resistive switching in 2D bismuth oxyhalide nanosheets for nonvolatile memory and emulation of leaky integrate-and-fire functions San Diego, Calif. [u.a.] volume:450 year:2014 pages:233-242 extent:10 |
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Enthalten in Resistive switching in 2D bismuth oxyhalide nanosheets for nonvolatile memory and emulation of leaky integrate-and-fire functions San Diego, Calif. [u.a.] volume:450 year:2014 pages:233-242 extent:10 |
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Resistive switching in 2D bismuth oxyhalide nanosheets for nonvolatile memory and emulation of leaky integrate-and-fire functions |
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Li, Guoxin @@aut@@ Gao, Xuyuan @@oth@@ Xiao, Yali @@oth@@ Liu, Shaoqiong @@oth@@ Peng, Shan @@oth@@ Li, Xuesong @@oth@@ Shi, Ying @@oth@@ Zhang, Yuee @@oth@@ Yu, Lei @@oth@@ Wu, Xiaogang @@oth@@ Yan, Pixi @@oth@@ Yan, Liping @@oth@@ Teng, Qiaoyang @@oth@@ Tong, Guangzhi @@oth@@ Li, Zejun @@oth@@ |
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Development of a live attenuated vaccine candidate against duck Tembusu viral disease |
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Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. |
abstractGer |
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. |
abstract_unstemmed |
Duck Tembusu virus (DTMUV) is a newly emerging pathogenic flavivirus that is causing massive economic loss in the Chinese duck industry. To obtain a live vaccine candidate against the disease, the DTMUV isolate FX2010 was passaged serially in chicken embryo fibroblasts (CEFs). Characterization of FX2010-180P revealed that it was unable to replicate efficiently in chicken embryonated eggs, nor intranasally infect mice or shelducks at high doses of 5.5log10 tissue culture infectious doses (TCID50). FX2010-180P did not induce clinical symptoms, or pathological lesions in ducks at a dose of 5.5log10 TCID50. The attenuation of FX2010-180P was due to 19 amino acid changes and 15 synonymous mutations. Importantly, FX2010-180P elicited good immune responses in ducks inoculated at low doses (3.5log10 TCID50) and provided complete protection against challenge with a virulent strain. These results indicate that FX2010-180P is a promising candidate live vaccine for prevention of duck Tembusu viral disease. |
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Development of a live attenuated vaccine candidate against duck Tembusu viral disease |
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|
score |
7.400717 |