Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification
Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infecti...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Jaroenram, Wansadaj [verfasserIn] |
|---|
| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2014transfer abstract |
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| Schlagwörter: |
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| Umfang: |
4 |
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| Übergeordnetes Werk: |
Enthalten in: Metamorphic - Xu, Nanqing ELSEVIER, 2022, London |
|---|---|
| Übergeordnetes Werk: |
volume:28 ; year:2014 ; number:5 ; pages:284-287 ; extent:4 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.mcp.2014.08.002 |
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| 520 | |a Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. | ||
| 520 | |a Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. | ||
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10.1016/j.mcp.2014.08.002 doi GBVA2014014000007.pica (DE-627)ELV022840397 (ELSEVIER)S0890-8508(14)00041-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 38.25 bkl 38.30 bkl 38.32 bkl Jaroenram, Wansadaj verfasserin aut Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Penaeus stylirostris Elsevier PstDV Elsevier RPA Elsevier PstDV-related sequences Elsevier Owens, Leigh oth Enthalten in Academic Press Xu, Nanqing ELSEVIER Metamorphic 2022 London (DE-627)ELV008152659 volume:28 year:2014 number:5 pages:284-287 extent:4 https://doi.org/10.1016/j.mcp.2014.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 38.25 Petrologie: Allgemeines VZ 38.30 Mineralogie VZ 38.32 Geochemie VZ AR 28 2014 5 284-287 4 045F 570 |
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10.1016/j.mcp.2014.08.002 doi GBVA2014014000007.pica (DE-627)ELV022840397 (ELSEVIER)S0890-8508(14)00041-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 38.25 bkl 38.30 bkl 38.32 bkl Jaroenram, Wansadaj verfasserin aut Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Penaeus stylirostris Elsevier PstDV Elsevier RPA Elsevier PstDV-related sequences Elsevier Owens, Leigh oth Enthalten in Academic Press Xu, Nanqing ELSEVIER Metamorphic 2022 London (DE-627)ELV008152659 volume:28 year:2014 number:5 pages:284-287 extent:4 https://doi.org/10.1016/j.mcp.2014.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 38.25 Petrologie: Allgemeines VZ 38.30 Mineralogie VZ 38.32 Geochemie VZ AR 28 2014 5 284-287 4 045F 570 |
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10.1016/j.mcp.2014.08.002 doi GBVA2014014000007.pica (DE-627)ELV022840397 (ELSEVIER)S0890-8508(14)00041-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 38.25 bkl 38.30 bkl 38.32 bkl Jaroenram, Wansadaj verfasserin aut Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Penaeus stylirostris Elsevier PstDV Elsevier RPA Elsevier PstDV-related sequences Elsevier Owens, Leigh oth Enthalten in Academic Press Xu, Nanqing ELSEVIER Metamorphic 2022 London (DE-627)ELV008152659 volume:28 year:2014 number:5 pages:284-287 extent:4 https://doi.org/10.1016/j.mcp.2014.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 38.25 Petrologie: Allgemeines VZ 38.30 Mineralogie VZ 38.32 Geochemie VZ AR 28 2014 5 284-287 4 045F 570 |
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10.1016/j.mcp.2014.08.002 doi GBVA2014014000007.pica (DE-627)ELV022840397 (ELSEVIER)S0890-8508(14)00041-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 38.25 bkl 38.30 bkl 38.32 bkl Jaroenram, Wansadaj verfasserin aut Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Penaeus stylirostris Elsevier PstDV Elsevier RPA Elsevier PstDV-related sequences Elsevier Owens, Leigh oth Enthalten in Academic Press Xu, Nanqing ELSEVIER Metamorphic 2022 London (DE-627)ELV008152659 volume:28 year:2014 number:5 pages:284-287 extent:4 https://doi.org/10.1016/j.mcp.2014.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 38.25 Petrologie: Allgemeines VZ 38.30 Mineralogie VZ 38.32 Geochemie VZ AR 28 2014 5 284-287 4 045F 570 |
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10.1016/j.mcp.2014.08.002 doi GBVA2014014000007.pica (DE-627)ELV022840397 (ELSEVIER)S0890-8508(14)00041-3 DE-627 ger DE-627 rakwb eng 570 610 570 DE-600 610 DE-600 550 VZ 38.25 bkl 38.30 bkl 38.32 bkl Jaroenram, Wansadaj verfasserin aut Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. Penaeus stylirostris Elsevier PstDV Elsevier RPA Elsevier PstDV-related sequences Elsevier Owens, Leigh oth Enthalten in Academic Press Xu, Nanqing ELSEVIER Metamorphic 2022 London (DE-627)ELV008152659 volume:28 year:2014 number:5 pages:284-287 extent:4 https://doi.org/10.1016/j.mcp.2014.08.002 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OPC-GGO 38.25 Petrologie: Allgemeines VZ 38.30 Mineralogie VZ 38.32 Geochemie VZ AR 28 2014 5 284-287 4 045F 570 |
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Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification |
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Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification |
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separation of endogenous viral elements from infectious penaeus stylirostris densovirus using recombinase polymerase amplification |
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Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification |
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Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. |
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Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. |
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Non-infectious Penaeus stylirostris densovirus (PstDV)-related sequences in the shrimp genome cause false positive results with current PCR protocols. Here, we examined and mapped PstDV insertion profile in the genome of Australian Penaeus monodon. A DNA sequence which is likely to represent infectious PstDV was also identified and used as a target sequence for recombinase polymerase amplification (RPA)-based approach, developed for specifically detecting PstDV. The RPA protocol at 37 °C for 30 min showed no cross-reaction with other shrimp viruses, and was 10 times more sensitive than the 309F/R PCR protocol currently recommended by the World Organization for Animal Health (OIE) for PstDV diagnosis. These features, together with the simplicity of the protocol, requiring only a heating block for the reaction, offer opportunities for rapid and efficient detection of PstDV. |
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Separation of endogenous viral elements from infectious Penaeus stylirostris densovirus using recombinase polymerase amplification |
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