Rapid and reliable species identification of scallops by MALDI-TOF mass spectrometry
Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and t...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Stephan, Roger [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2014transfer abstract |
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| Schlagwörter: |
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| Umfang: |
4 |
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| Übergeordnetes Werk: |
Enthalten in: Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma - Tanaka, Hajime ELSEVIER, 2022, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:46 ; year:2014 ; pages:6-9 ; extent:4 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.foodcont.2014.04.047 |
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ELV022890521 |
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| 245 | 1 | 0 | |a Rapid and reliable species identification of scallops by MALDI-TOF mass spectrometry |
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| 520 | |a Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. | ||
| 520 | |a Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. | ||
| 650 | 7 | |a MALDI-TOF MS |2 Elsevier | |
| 650 | 7 | |a Scallops |2 Elsevier | |
| 650 | 7 | |a Mislabeling |2 Elsevier | |
| 650 | 7 | |a Species identification |2 Elsevier | |
| 700 | 1 | |a Johler, Sophia |4 oth | |
| 700 | 1 | |a Oesterle, Noémie |4 oth | |
| 700 | 1 | |a Näumann, Gabriele |4 oth | |
| 700 | 1 | |a Vogel, Guido |4 oth | |
| 700 | 1 | |a Pflüger, Valentin |4 oth | |
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10.1016/j.foodcont.2014.04.047 doi GBVA2014016000018.pica (DE-627)ELV022890521 (ELSEVIER)S0956-7135(14)00244-8 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Stephan, Roger verfasserin aut Rapid and reliable species identification of scallops by MALDI-TOF mass spectrometry 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. MALDI-TOF MS Elsevier Scallops Elsevier Mislabeling Elsevier Species identification Elsevier Johler, Sophia oth Oesterle, Noémie oth Näumann, Gabriele oth Vogel, Guido oth Pflüger, Valentin oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:46 year:2014 pages:6-9 extent:4 https://doi.org/10.1016/j.foodcont.2014.04.047 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 46 2014 6-9 4 045F 630 |
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10.1016/j.foodcont.2014.04.047 doi GBVA2014016000018.pica (DE-627)ELV022890521 (ELSEVIER)S0956-7135(14)00244-8 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Stephan, Roger verfasserin aut Rapid and reliable species identification of scallops by MALDI-TOF mass spectrometry 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. MALDI-TOF MS Elsevier Scallops Elsevier Mislabeling Elsevier Species identification Elsevier Johler, Sophia oth Oesterle, Noémie oth Näumann, Gabriele oth Vogel, Guido oth Pflüger, Valentin oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:46 year:2014 pages:6-9 extent:4 https://doi.org/10.1016/j.foodcont.2014.04.047 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 46 2014 6-9 4 045F 630 |
| allfields_unstemmed |
10.1016/j.foodcont.2014.04.047 doi GBVA2014016000018.pica (DE-627)ELV022890521 (ELSEVIER)S0956-7135(14)00244-8 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Stephan, Roger verfasserin aut Rapid and reliable species identification of scallops by MALDI-TOF mass spectrometry 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. MALDI-TOF MS Elsevier Scallops Elsevier Mislabeling Elsevier Species identification Elsevier Johler, Sophia oth Oesterle, Noémie oth Näumann, Gabriele oth Vogel, Guido oth Pflüger, Valentin oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:46 year:2014 pages:6-9 extent:4 https://doi.org/10.1016/j.foodcont.2014.04.047 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 46 2014 6-9 4 045F 630 |
| allfieldsGer |
10.1016/j.foodcont.2014.04.047 doi GBVA2014016000018.pica (DE-627)ELV022890521 (ELSEVIER)S0956-7135(14)00244-8 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Stephan, Roger verfasserin aut Rapid and reliable species identification of scallops by MALDI-TOF mass spectrometry 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. MALDI-TOF MS Elsevier Scallops Elsevier Mislabeling Elsevier Species identification Elsevier Johler, Sophia oth Oesterle, Noémie oth Näumann, Gabriele oth Vogel, Guido oth Pflüger, Valentin oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:46 year:2014 pages:6-9 extent:4 https://doi.org/10.1016/j.foodcont.2014.04.047 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 46 2014 6-9 4 045F 630 |
| allfieldsSound |
10.1016/j.foodcont.2014.04.047 doi GBVA2014016000018.pica (DE-627)ELV022890521 (ELSEVIER)S0956-7135(14)00244-8 DE-627 ger DE-627 rakwb eng 630 640 630 DE-600 640 DE-600 610 VZ Stephan, Roger verfasserin aut Rapid and reliable species identification of scallops by MALDI-TOF mass spectrometry 2014transfer abstract 4 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. MALDI-TOF MS Elsevier Scallops Elsevier Mislabeling Elsevier Species identification Elsevier Johler, Sophia oth Oesterle, Noémie oth Näumann, Gabriele oth Vogel, Guido oth Pflüger, Valentin oth Enthalten in Elsevier Science Tanaka, Hajime ELSEVIER Defining Tumour Shape Irregularity for Preoperative Risk Stratification of Clinically Localised Renal Cell Carcinoma 2022 Amsterdam [u.a.] (DE-627)ELV009139680 volume:46 year:2014 pages:6-9 extent:4 https://doi.org/10.1016/j.foodcont.2014.04.047 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 46 2014 6-9 4 045F 630 |
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Rapid and reliable species identification of scallops by MALDI-TOF mass spectrometry |
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Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. |
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Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. |
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Correct identification of scallop species is of crucial importance to the seafood industry to assess marketability of the product, as well as to prevent unintentional mislabeling and to comply with current legislative guidelines. However, various scallop species are virtually indistinguishable and to date there is no fast, reproducible, and inexpensive technique for species identification of scallops. A new promising tool in the identification of various organisms is the matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS). In this study, we aimed to 1) evaluate the possibility of MALDI-TOF MS as a tool for rapid and reliable species identification of scallops and 2) assess the occurrence of mislabeled scallops sold in Switzerland. We therefore used 16 scallop reference samples, as well as scallops sampled at eight Swiss retail facilities and identified the scallop species both by 16S rRNA PCR and MALDI-TOF MS. For all tested scallop species, MALDI-TOF MS yielded reproducible and unique mass spectra profiles covering a wide molecular mass range of 3000–30,000 Da. Species-specific biomarker protein mass patterns were determined. Species identification of reference scallop samples using MALDI-TOF MS was in accordance with species identification using the established 16S rRNA PCR tool. Identification of the scallop samples purchased in Switzerland also yielded identical results for MALDI-TOF MS and the 16S RNA PCR approach. We were able to show that several of the scallop samples were labeled incorrectly. All mislabeled scallops belonged to the species Placopecten magellanicus, but were marketed as “Jacobsmuschel”, a commercial name reserved for Pecten spp. In this study, we show that MALDI-TOF MS allows for rapid, reliable, and highly accurate species identification of scallops. |
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