Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana
In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chlo...
Ausführliche Beschreibung
Autor*in: |
Aslan, Selcuk [verfasserIn] |
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E-Artikel |
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Sprache: |
Englisch |
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2014transfer abstract |
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Umfang: |
10 |
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Übergeordnetes Werk: |
Enthalten in: Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery - Gao, Mengyao ELSEVIER, 2014transfer abstract, Orlando, Fla |
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Übergeordnetes Werk: |
volume:25 ; year:2014 ; pages:103-112 ; extent:10 |
Links: |
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DOI / URN: |
10.1016/j.ymben.2014.07.001 |
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520 | |a In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. | ||
650 | 7 | |a Fatty acyl reductase |2 Elsevier | |
650 | 7 | |a Fatty alcohol |2 Elsevier | |
650 | 7 | |a Nicotiana benthamiana |2 Elsevier | |
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700 | 1 | |a Hofvander, Per |4 oth | |
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10.1016/j.ymben.2014.07.001 doi GBVA2014018000023.pica (DE-627)ELV02297590X (ELSEVIER)S1096-7176(14)00092-5 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Aslan, Selcuk verfasserin aut Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. Fatty acyl reductase Elsevier Fatty alcohol Elsevier Nicotiana benthamiana Elsevier Wax ester Elsevier Wax ester synthase Elsevier Chloroplast Elsevier Sun, Chuanxin oth Leonova, Svetlana oth Dutta, Paresh oth Dörmann, Peter oth Domergue, Frédéric oth Stymne, Sten oth Hofvander, Per oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:25 year:2014 pages:103-112 extent:10 https://doi.org/10.1016/j.ymben.2014.07.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 25 2014 103-112 10 045F 610 |
spelling |
10.1016/j.ymben.2014.07.001 doi GBVA2014018000023.pica (DE-627)ELV02297590X (ELSEVIER)S1096-7176(14)00092-5 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Aslan, Selcuk verfasserin aut Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. Fatty acyl reductase Elsevier Fatty alcohol Elsevier Nicotiana benthamiana Elsevier Wax ester Elsevier Wax ester synthase Elsevier Chloroplast Elsevier Sun, Chuanxin oth Leonova, Svetlana oth Dutta, Paresh oth Dörmann, Peter oth Domergue, Frédéric oth Stymne, Sten oth Hofvander, Per oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:25 year:2014 pages:103-112 extent:10 https://doi.org/10.1016/j.ymben.2014.07.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 25 2014 103-112 10 045F 610 |
allfields_unstemmed |
10.1016/j.ymben.2014.07.001 doi GBVA2014018000023.pica (DE-627)ELV02297590X (ELSEVIER)S1096-7176(14)00092-5 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Aslan, Selcuk verfasserin aut Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. Fatty acyl reductase Elsevier Fatty alcohol Elsevier Nicotiana benthamiana Elsevier Wax ester Elsevier Wax ester synthase Elsevier Chloroplast Elsevier Sun, Chuanxin oth Leonova, Svetlana oth Dutta, Paresh oth Dörmann, Peter oth Domergue, Frédéric oth Stymne, Sten oth Hofvander, Per oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:25 year:2014 pages:103-112 extent:10 https://doi.org/10.1016/j.ymben.2014.07.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 25 2014 103-112 10 045F 610 |
allfieldsGer |
10.1016/j.ymben.2014.07.001 doi GBVA2014018000023.pica (DE-627)ELV02297590X (ELSEVIER)S1096-7176(14)00092-5 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Aslan, Selcuk verfasserin aut Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. Fatty acyl reductase Elsevier Fatty alcohol Elsevier Nicotiana benthamiana Elsevier Wax ester Elsevier Wax ester synthase Elsevier Chloroplast Elsevier Sun, Chuanxin oth Leonova, Svetlana oth Dutta, Paresh oth Dörmann, Peter oth Domergue, Frédéric oth Stymne, Sten oth Hofvander, Per oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:25 year:2014 pages:103-112 extent:10 https://doi.org/10.1016/j.ymben.2014.07.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 25 2014 103-112 10 045F 610 |
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10.1016/j.ymben.2014.07.001 doi GBVA2014018000023.pica (DE-627)ELV02297590X (ELSEVIER)S1096-7176(14)00092-5 DE-627 ger DE-627 rakwb eng 610 610 DE-600 540 VZ 610 VZ 44.00 bkl Aslan, Selcuk verfasserin aut Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana 2014transfer abstract 10 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. Fatty acyl reductase Elsevier Fatty alcohol Elsevier Nicotiana benthamiana Elsevier Wax ester Elsevier Wax ester synthase Elsevier Chloroplast Elsevier Sun, Chuanxin oth Leonova, Svetlana oth Dutta, Paresh oth Dörmann, Peter oth Domergue, Frédéric oth Stymne, Sten oth Hofvander, Per oth Enthalten in Academic Press Gao, Mengyao ELSEVIER Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery 2014transfer abstract Orlando, Fla (DE-627)ELV012555568 volume:25 year:2014 pages:103-112 extent:10 https://doi.org/10.1016/j.ymben.2014.07.001 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA GBV_ILN_20 GBV_ILN_22 GBV_ILN_40 GBV_ILN_70 44.00 Medizin: Allgemeines VZ AR 25 2014 103-112 10 045F 610 |
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Enthalten in Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery Orlando, Fla volume:25 year:2014 pages:103-112 extent:10 |
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Enthalten in Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery Orlando, Fla volume:25 year:2014 pages:103-112 extent:10 |
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Inhibition on polysulfides dissolve during the discharge-charge by using fish-scale-based porous carbon for lithium-sulfur battery |
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Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana |
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In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. |
abstractGer |
In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. |
abstract_unstemmed |
In a future bio-based economy, renewable sources for lipid compounds at attractive cost are needed for applications where today petrochemical derivatives are dominating. Wax esters and fatty alcohols provide diverse industrial uses, such as in lubricant and surfactant production. In this study, chloroplast metabolism was engineered to divert intermediates from de novo fatty acid biosynthesis to wax ester synthesis. To accomplish this, chloroplast targeted fatty acyl reductases (FAR) and wax ester synthases (WS) were transiently expressed in Nicotiana benthamiana leaves. Wax esters of different qualities and quantities were produced providing insights to the properties and interaction of the individual enzymes used. In particular, a phytyl ester synthase was found to be a premium candidate for medium chain wax ester synthesis. Catalytic activities of FAR and WS were also expressed as a fusion protein and determined functionally equivalent to the expression of individual enzymes for wax ester synthesis in chloroplasts. |
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Wax esters of different compositions produced via engineering of leaf chloroplast metabolism in Nicotiana benthamiana |
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