Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates
Highlights • Immobilization approach based on biotinylated oligo/streptavidin conjugates. • Enhanced oligonucleotides immobilization efficiency and detection sensitivity. • Improved hybridization kinetics and discrimination ability of mutations. • Microarray for detection of seven most common BRCA1...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Mavrogiannopoulou, E. [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015 |
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| Schlagwörter: |
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| Umfang: |
9 |
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| Übergeordnetes Werk: |
Enthalten in: Pharmacy students' perceptions toward peer assessment and its use in teaching patient presentation skills - Han, Zhe ELSEVIER, 2019, an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin, Amsterdam [u.a.] |
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| Übergeordnetes Werk: |
volume:128 ; year:2015 ; day:1 ; month:04 ; pages:464-472 ; extent:9 |
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| DOI / URN: |
10.1016/j.colsurfb.2015.02.045 |
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ELV023797630 |
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| 520 | |a Highlights • Immobilization approach based on biotinylated oligo/streptavidin conjugates. • Enhanced oligonucleotides immobilization efficiency and detection sensitivity. • Improved hybridization kinetics and discrimination ability of mutations. • Microarray for detection of seven most common BRCA1 gene mutations in 40min. | ||
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10.1016/j.colsurfb.2015.02.045 doi GBV00000000000462.pica (DE-627)ELV023797630 (ELSEVIER)S0927-7765(15)00129-0 DE-627 ger DE-627 rakwb eng 540 VZ Mavrogiannopoulou, E. verfasserin aut Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates 2015 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Highlights • Immobilization approach based on biotinylated oligo/streptavidin conjugates. • Enhanced oligonucleotides immobilization efficiency and detection sensitivity. • Improved hybridization kinetics and discrimination ability of mutations. • Microarray for detection of seven most common BRCA1 gene mutations in 40min. Biotinylated oligonucleotides Elsevier Microarrays Elsevier BRCA1 gene mutations Elsevier Streptavidin Elsevier Oligonucleotide immobilization Elsevier Petrou, P.S. oth Koukouvinos, G. oth Yannoukakos, D. oth Siafaka-Kapadai, A. oth Fornal, K. oth Awsiuk, K. oth Budkowski, A. oth Kakabakos, S.E. oth Enthalten in Elsevier Science Han, Zhe ELSEVIER Pharmacy students' perceptions toward peer assessment and its use in teaching patient presentation skills 2019 an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin Amsterdam [u.a.] (DE-627)ELV003763382 volume:128 year:2015 day:1 month:04 pages:464-472 extent:9 https://doi.org/10.1016/j.colsurfb.2015.02.045 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 128 2015 1 0401 464-472 9 |
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10.1016/j.colsurfb.2015.02.045 doi GBV00000000000462.pica (DE-627)ELV023797630 (ELSEVIER)S0927-7765(15)00129-0 DE-627 ger DE-627 rakwb eng 540 VZ Mavrogiannopoulou, E. verfasserin aut Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates 2015 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Highlights • Immobilization approach based on biotinylated oligo/streptavidin conjugates. • Enhanced oligonucleotides immobilization efficiency and detection sensitivity. • Improved hybridization kinetics and discrimination ability of mutations. • Microarray for detection of seven most common BRCA1 gene mutations in 40min. Biotinylated oligonucleotides Elsevier Microarrays Elsevier BRCA1 gene mutations Elsevier Streptavidin Elsevier Oligonucleotide immobilization Elsevier Petrou, P.S. oth Koukouvinos, G. oth Yannoukakos, D. oth Siafaka-Kapadai, A. oth Fornal, K. oth Awsiuk, K. oth Budkowski, A. oth Kakabakos, S.E. oth Enthalten in Elsevier Science Han, Zhe ELSEVIER Pharmacy students' perceptions toward peer assessment and its use in teaching patient presentation skills 2019 an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin Amsterdam [u.a.] (DE-627)ELV003763382 volume:128 year:2015 day:1 month:04 pages:464-472 extent:9 https://doi.org/10.1016/j.colsurfb.2015.02.045 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 128 2015 1 0401 464-472 9 |
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10.1016/j.colsurfb.2015.02.045 doi GBV00000000000462.pica (DE-627)ELV023797630 (ELSEVIER)S0927-7765(15)00129-0 DE-627 ger DE-627 rakwb eng 540 VZ Mavrogiannopoulou, E. verfasserin aut Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates 2015 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Highlights • Immobilization approach based on biotinylated oligo/streptavidin conjugates. • Enhanced oligonucleotides immobilization efficiency and detection sensitivity. • Improved hybridization kinetics and discrimination ability of mutations. • Microarray for detection of seven most common BRCA1 gene mutations in 40min. Biotinylated oligonucleotides Elsevier Microarrays Elsevier BRCA1 gene mutations Elsevier Streptavidin Elsevier Oligonucleotide immobilization Elsevier Petrou, P.S. oth Koukouvinos, G. oth Yannoukakos, D. oth Siafaka-Kapadai, A. oth Fornal, K. oth Awsiuk, K. oth Budkowski, A. oth Kakabakos, S.E. oth Enthalten in Elsevier Science Han, Zhe ELSEVIER Pharmacy students' perceptions toward peer assessment and its use in teaching patient presentation skills 2019 an international journal devoted to fundamental and applied research on colloid and interfacial phenomena in relation to systems of biological origin Amsterdam [u.a.] (DE-627)ELV003763382 volume:128 year:2015 day:1 month:04 pages:464-472 extent:9 https://doi.org/10.1016/j.colsurfb.2015.02.045 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA AR 128 2015 1 0401 464-472 9 |
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2024-07-06T19:45:11.134Z |
| _version_ |
1803860166453493760 |
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