IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation
Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Huang, Zhilin [verfasserIn] |
|---|
| Format: |
E-Artikel |
|---|---|
| Sprache: |
Englisch |
| Erschienen: |
2015transfer abstract |
|---|
| Schlagwörter: |
|---|
| Umfang: |
9 |
|---|
| Übergeordnetes Werk: |
Enthalten in: Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention - Istanbuly, Sedralmontaha ELSEVIER, 2021, London |
|---|---|
| Übergeordnetes Werk: |
volume:46 ; year:2015 ; number:2 ; pages:477-485 ; extent:9 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.fsi.2015.07.020 |
|---|
| Katalog-ID: |
ELV023874988 |
|---|
| LEADER | 01000caa a22002652 4500 | ||
|---|---|---|---|
| 001 | ELV023874988 | ||
| 003 | DE-627 | ||
| 005 | 20230625141816.0 | ||
| 007 | cr uuu---uuuuu | ||
| 008 | 180603s2015 xx |||||o 00| ||eng c | ||
| 024 | 7 | |a 10.1016/j.fsi.2015.07.020 |2 doi | |
| 028 | 5 | 2 | |a GBVA2015018000028.pica |
| 035 | |a (DE-627)ELV023874988 | ||
| 035 | |a (ELSEVIER)S1050-4648(15)30083-8 | ||
| 040 | |a DE-627 |b ger |c DE-627 |e rakwb | ||
| 041 | |a eng | ||
| 082 | 0 | |a 630 | |
| 082 | 0 | 4 | |a 630 |q DE-600 |
| 082 | 0 | 4 | |a 610 |q VZ |
| 084 | |a 44.85 |2 bkl | ||
| 100 | 1 | |a Huang, Zhilin |e verfasserin |4 aut | |
| 245 | 1 | 0 | |a IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation |
| 264 | 1 | |c 2015transfer abstract | |
| 300 | |a 9 | ||
| 336 | |a nicht spezifiziert |b zzz |2 rdacontent | ||
| 337 | |a nicht spezifiziert |b z |2 rdamedia | ||
| 338 | |a nicht spezifiziert |b zu |2 rdacarrier | ||
| 520 | |a Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. | ||
| 520 | |a Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. | ||
| 650 | 7 | |a IFN |2 Elsevier | |
| 650 | 7 | |a SVCV |2 Elsevier | |
| 650 | 7 | |a Innate immunity |2 Elsevier | |
| 650 | 7 | |a Black carp |2 Elsevier | |
| 700 | 1 | |a Chen, Song |4 oth | |
| 700 | 1 | |a Liu, Jiachen |4 oth | |
| 700 | 1 | |a Xiao, Jun |4 oth | |
| 700 | 1 | |a Yan, Jun |4 oth | |
| 700 | 1 | |a Feng, Hao |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Academic Press |a Istanbuly, Sedralmontaha ELSEVIER |t Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention |d 2021 |g London |w (DE-627)ELV006540406 |
| 773 | 1 | 8 | |g volume:46 |g year:2015 |g number:2 |g pages:477-485 |g extent:9 |
| 856 | 4 | 0 | |u https://doi.org/10.1016/j.fsi.2015.07.020 |3 Volltext |
| 912 | |a GBV_USEFLAG_U | ||
| 912 | |a GBV_ELV | ||
| 912 | |a SYSFLAG_U | ||
| 912 | |a SSG-OLC-PHA | ||
| 936 | b | k | |a 44.85 |j Kardiologie |j Angiologie |q VZ |
| 951 | |a AR | ||
| 952 | |d 46 |j 2015 |e 2 |h 477-485 |g 9 | ||
| 953 | |2 045F |a 630 | ||
| author_variant |
z h zh |
|---|---|
| matchkey_str |
huangzhilinchensongliujiachenxiaojunyanj:2015----:faflccriaatvrlyoieoiidihl |
| hierarchy_sort_str |
2015transfer abstract |
| bklnumber |
44.85 |
| publishDate |
2015 |
| allfields |
10.1016/j.fsi.2015.07.020 doi GBVA2015018000028.pica (DE-627)ELV023874988 (ELSEVIER)S1050-4648(15)30083-8 DE-627 ger DE-627 rakwb eng 630 630 DE-600 610 VZ 44.85 bkl Huang, Zhilin verfasserin aut IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation 2015transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp Elsevier Chen, Song oth Liu, Jiachen oth Xiao, Jun oth Yan, Jun oth Feng, Hao oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:46 year:2015 number:2 pages:477-485 extent:9 https://doi.org/10.1016/j.fsi.2015.07.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 46 2015 2 477-485 9 045F 630 |
| spelling |
10.1016/j.fsi.2015.07.020 doi GBVA2015018000028.pica (DE-627)ELV023874988 (ELSEVIER)S1050-4648(15)30083-8 DE-627 ger DE-627 rakwb eng 630 630 DE-600 610 VZ 44.85 bkl Huang, Zhilin verfasserin aut IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation 2015transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp Elsevier Chen, Song oth Liu, Jiachen oth Xiao, Jun oth Yan, Jun oth Feng, Hao oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:46 year:2015 number:2 pages:477-485 extent:9 https://doi.org/10.1016/j.fsi.2015.07.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 46 2015 2 477-485 9 045F 630 |
| allfields_unstemmed |
10.1016/j.fsi.2015.07.020 doi GBVA2015018000028.pica (DE-627)ELV023874988 (ELSEVIER)S1050-4648(15)30083-8 DE-627 ger DE-627 rakwb eng 630 630 DE-600 610 VZ 44.85 bkl Huang, Zhilin verfasserin aut IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation 2015transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp Elsevier Chen, Song oth Liu, Jiachen oth Xiao, Jun oth Yan, Jun oth Feng, Hao oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:46 year:2015 number:2 pages:477-485 extent:9 https://doi.org/10.1016/j.fsi.2015.07.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 46 2015 2 477-485 9 045F 630 |
| allfieldsGer |
10.1016/j.fsi.2015.07.020 doi GBVA2015018000028.pica (DE-627)ELV023874988 (ELSEVIER)S1050-4648(15)30083-8 DE-627 ger DE-627 rakwb eng 630 630 DE-600 610 VZ 44.85 bkl Huang, Zhilin verfasserin aut IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation 2015transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp Elsevier Chen, Song oth Liu, Jiachen oth Xiao, Jun oth Yan, Jun oth Feng, Hao oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:46 year:2015 number:2 pages:477-485 extent:9 https://doi.org/10.1016/j.fsi.2015.07.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 46 2015 2 477-485 9 045F 630 |
| allfieldsSound |
10.1016/j.fsi.2015.07.020 doi GBVA2015018000028.pica (DE-627)ELV023874988 (ELSEVIER)S1050-4648(15)30083-8 DE-627 ger DE-627 rakwb eng 630 630 DE-600 610 VZ 44.85 bkl Huang, Zhilin verfasserin aut IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation 2015transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp Elsevier Chen, Song oth Liu, Jiachen oth Xiao, Jun oth Yan, Jun oth Feng, Hao oth Enthalten in Academic Press Istanbuly, Sedralmontaha ELSEVIER Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention 2021 London (DE-627)ELV006540406 volume:46 year:2015 number:2 pages:477-485 extent:9 https://doi.org/10.1016/j.fsi.2015.07.020 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.85 Kardiologie Angiologie VZ AR 46 2015 2 477-485 9 045F 630 |
| language |
English |
| source |
Enthalten in Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention London volume:46 year:2015 number:2 pages:477-485 extent:9 |
| sourceStr |
Enthalten in Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention London volume:46 year:2015 number:2 pages:477-485 extent:9 |
| format_phy_str_mv |
Article |
| bklname |
Kardiologie Angiologie |
| institution |
findex.gbv.de |
| topic_facet |
IFN SVCV Innate immunity Black carp |
| dewey-raw |
630 |
| isfreeaccess_bool |
false |
| container_title |
Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention |
| authorswithroles_txt_mv |
Huang, Zhilin @@aut@@ Chen, Song @@oth@@ Liu, Jiachen @@oth@@ Xiao, Jun @@oth@@ Yan, Jun @@oth@@ Feng, Hao @@oth@@ |
| publishDateDaySort_date |
2015-01-01T00:00:00Z |
| hierarchy_top_id |
ELV006540406 |
| dewey-sort |
3630 |
| id |
ELV023874988 |
| language_de |
englisch |
| fullrecord |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV023874988</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230625141816.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180603s2015 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.fsi.2015.07.020</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBVA2015018000028.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV023874988</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S1050-4648(15)30083-8</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">630</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">630</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.85</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Huang, Zhilin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">9</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">IFN</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">SVCV</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Innate immunity</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Black carp</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Song</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Jiachen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xiao, Jun</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yan, Jun</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Feng, Hao</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Academic Press</subfield><subfield code="a">Istanbuly, Sedralmontaha ELSEVIER</subfield><subfield code="t">Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention</subfield><subfield code="d">2021</subfield><subfield code="g">London</subfield><subfield code="w">(DE-627)ELV006540406</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:46</subfield><subfield code="g">year:2015</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:477-485</subfield><subfield code="g">extent:9</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.fsi.2015.07.020</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.85</subfield><subfield code="j">Kardiologie</subfield><subfield code="j">Angiologie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">46</subfield><subfield code="j">2015</subfield><subfield code="e">2</subfield><subfield code="h">477-485</subfield><subfield code="g">9</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">630</subfield></datafield></record></collection>
|
| author |
Huang, Zhilin |
| spellingShingle |
Huang, Zhilin ddc 630 ddc 610 bkl 44.85 Elsevier IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation |
| authorStr |
Huang, Zhilin |
| ppnlink_with_tag_str_mv |
@@773@@(DE-627)ELV006540406 |
| format |
electronic Article |
| dewey-ones |
630 - Agriculture & related technologies 610 - Medicine & health |
| delete_txt_mv |
keep |
| author_role |
aut |
| collection |
elsevier |
| remote_str |
true |
| illustrated |
Not Illustrated |
| topic_title |
630 630 DE-600 610 VZ 44.85 bkl IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp Elsevier |
| topic |
ddc 630 ddc 610 bkl 44.85 Elsevier IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp |
| topic_unstemmed |
ddc 630 ddc 610 bkl 44.85 Elsevier IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp |
| topic_browse |
ddc 630 ddc 610 bkl 44.85 Elsevier IFN Elsevier SVCV Elsevier Innate immunity Elsevier Black carp |
| format_facet |
Elektronische Aufsätze Aufsätze Elektronische Ressource |
| format_main_str_mv |
Text Zeitschrift/Artikel |
| carriertype_str_mv |
zu |
| author2_variant |
s c sc j l jl j x jx j y jy h f hf |
| hierarchy_parent_title |
Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention |
| hierarchy_parent_id |
ELV006540406 |
| dewey-tens |
630 - Agriculture 610 - Medicine & health |
| hierarchy_top_title |
Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention |
| isfreeaccess_txt |
false |
| familylinks_str_mv |
(DE-627)ELV006540406 |
| title |
IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation |
| ctrlnum |
(DE-627)ELV023874988 (ELSEVIER)S1050-4648(15)30083-8 |
| title_full |
IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation |
| author_sort |
Huang, Zhilin |
| journal |
Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention |
| journalStr |
Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention |
| lang_code |
eng |
| isOA_bool |
false |
| dewey-hundreds |
600 - Technology |
| recordtype |
marc |
| publishDateSort |
2015 |
| contenttype_str_mv |
zzz |
| container_start_page |
477 |
| author_browse |
Huang, Zhilin |
| container_volume |
46 |
| physical |
9 |
| class |
630 630 DE-600 610 VZ 44.85 bkl |
| format_se |
Elektronische Aufsätze |
| author-letter |
Huang, Zhilin |
| doi_str_mv |
10.1016/j.fsi.2015.07.020 |
| dewey-full |
630 610 |
| title_sort |
ifna of black carp is an antiviral cytokine modified with n-linked glycosylation |
| title_auth |
IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation |
| abstract |
Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. |
| abstractGer |
Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. |
| abstract_unstemmed |
Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine. |
| collection_details |
GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA |
| container_issue |
2 |
| title_short |
IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation |
| url |
https://doi.org/10.1016/j.fsi.2015.07.020 |
| remote_bool |
true |
| author2 |
Chen, Song Liu, Jiachen Xiao, Jun Yan, Jun Feng, Hao |
| author2Str |
Chen, Song Liu, Jiachen Xiao, Jun Yan, Jun Feng, Hao |
| ppnlink |
ELV006540406 |
| mediatype_str_mv |
z |
| isOA_txt |
false |
| hochschulschrift_bool |
false |
| author2_role |
oth oth oth oth oth |
| doi_str |
10.1016/j.fsi.2015.07.020 |
| up_date |
2024-07-06T19:57:03.463Z |
| _version_ |
1803860913384587264 |
| fullrecord_marcxml |
<?xml version="1.0" encoding="UTF-8"?><collection xmlns="http://www.loc.gov/MARC21/slim"><record><leader>01000caa a22002652 4500</leader><controlfield tag="001">ELV023874988</controlfield><controlfield tag="003">DE-627</controlfield><controlfield tag="005">20230625141816.0</controlfield><controlfield tag="007">cr uuu---uuuuu</controlfield><controlfield tag="008">180603s2015 xx |||||o 00| ||eng c</controlfield><datafield tag="024" ind1="7" ind2=" "><subfield code="a">10.1016/j.fsi.2015.07.020</subfield><subfield code="2">doi</subfield></datafield><datafield tag="028" ind1="5" ind2="2"><subfield code="a">GBVA2015018000028.pica</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(DE-627)ELV023874988</subfield></datafield><datafield tag="035" ind1=" " ind2=" "><subfield code="a">(ELSEVIER)S1050-4648(15)30083-8</subfield></datafield><datafield tag="040" ind1=" " ind2=" "><subfield code="a">DE-627</subfield><subfield code="b">ger</subfield><subfield code="c">DE-627</subfield><subfield code="e">rakwb</subfield></datafield><datafield tag="041" ind1=" " ind2=" "><subfield code="a">eng</subfield></datafield><datafield tag="082" ind1="0" ind2=" "><subfield code="a">630</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">630</subfield><subfield code="q">DE-600</subfield></datafield><datafield tag="082" ind1="0" ind2="4"><subfield code="a">610</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="084" ind1=" " ind2=" "><subfield code="a">44.85</subfield><subfield code="2">bkl</subfield></datafield><datafield tag="100" ind1="1" ind2=" "><subfield code="a">Huang, Zhilin</subfield><subfield code="e">verfasserin</subfield><subfield code="4">aut</subfield></datafield><datafield tag="245" ind1="1" ind2="0"><subfield code="a">IFNa of black carp is an antiviral cytokine modified with N-linked glycosylation</subfield></datafield><datafield tag="264" ind1=" " ind2="1"><subfield code="c">2015transfer abstract</subfield></datafield><datafield tag="300" ind1=" " ind2=" "><subfield code="a">9</subfield></datafield><datafield tag="336" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zzz</subfield><subfield code="2">rdacontent</subfield></datafield><datafield tag="337" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">z</subfield><subfield code="2">rdamedia</subfield></datafield><datafield tag="338" ind1=" " ind2=" "><subfield code="a">nicht spezifiziert</subfield><subfield code="b">zu</subfield><subfield code="2">rdacarrier</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Type I interferons (IFNs) play an important role in the antiviral immune response in teleost fish. In this study, one type I interferon (bcIFNa) of black carp (Mylopharyngodon piceus) has been cloned and characterized. The full-length cDNA of bcIFNa gene consists of 783 nucleotides and the predicted bcIFNa protein contains 185 amino acids. Semi-quantitative RT-PCR analysis demonstrated that bcIFNa mRNA transcription level in all the selected tissues of black carp was greatly increased at 33 h post spring viremia of carp virus (SVCV) infection. The protein of bcIFNa could be detected in both the whole cell lysate and the supernatant media of HEK293T cells transfected with plasmids expressing bcIFNa through immunoblot assay. EPC cells showed greatly increased antiviral ability when the cells were treated with the bcIFNa-containing conditioned media for 24 h before SVCV infection. Mass spectrum assay and glycosidase digestion analysis determined that bcIFNa is modified with N-linked glycosylation, which occurs on the Asn (N) of 38 site of this cytokine. The un-glycosylated mutant bcIFNa-N38Q could be secreted out of the cell and showed the similar antiviral ability against SVCV as that of wild type bcIFNa, which suggested that N-linked glycosylation does not contribute directly to the antiviral property of this fish cytokine.</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">IFN</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">SVCV</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Innate immunity</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="650" ind1=" " ind2="7"><subfield code="a">Black carp</subfield><subfield code="2">Elsevier</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Song</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Liu, Jiachen</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Xiao, Jun</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Yan, Jun</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Feng, Hao</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Academic Press</subfield><subfield code="a">Istanbuly, Sedralmontaha ELSEVIER</subfield><subfield code="t">Comparison of Outcomes of Patients With Versus Without Chronic Liver Disease Undergoing Percutaneous Coronary Intervention</subfield><subfield code="d">2021</subfield><subfield code="g">London</subfield><subfield code="w">(DE-627)ELV006540406</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:46</subfield><subfield code="g">year:2015</subfield><subfield code="g">number:2</subfield><subfield code="g">pages:477-485</subfield><subfield code="g">extent:9</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.fsi.2015.07.020</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SSG-OLC-PHA</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">44.85</subfield><subfield code="j">Kardiologie</subfield><subfield code="j">Angiologie</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">46</subfield><subfield code="j">2015</subfield><subfield code="e">2</subfield><subfield code="h">477-485</subfield><subfield code="g">9</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">630</subfield></datafield></record></collection>
|
| score |
7.3968735 |