Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care
Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the so...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Schmalenberg, Michael [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2015transfer abstract |
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| Umfang: |
6 |
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| Übergeordnetes Werk: |
Enthalten in: 969: Cesarean prevalence rates overtime by maternal characteristics - Thompson, Jennifer A. ELSEVIER, 2018, JIM, Amsterdam [u.a.] |
|---|---|
| Übergeordnetes Werk: |
volume:427 ; year:2015 ; pages:36-41 ; extent:6 |
| Links: |
|---|
| DOI / URN: |
10.1016/j.jim.2015.09.004 |
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ELV024016535 |
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| 520 | |a Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. | ||
| 520 | |a Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. | ||
| 700 | 1 | |a Beaudoin, Christopher |4 oth | |
| 700 | 1 | |a Bulst, Ludwig |4 oth | |
| 700 | 1 | |a Steubl, Dominik |4 oth | |
| 700 | 1 | |a Luppa, Peter B. |4 oth | |
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10.1016/j.jim.2015.09.004 doi GBVA2015022000017.pica (DE-627)ELV024016535 (ELSEVIER)S0022-1759(15)30046-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Schmalenberg, Michael verfasserin aut Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Beaudoin, Christopher oth Bulst, Ludwig oth Steubl, Dominik oth Luppa, Peter B. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:427 year:2015 pages:36-41 extent:6 https://doi.org/10.1016/j.jim.2015.09.004 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 427 2015 36-41 6 045F 610 |
| spelling |
10.1016/j.jim.2015.09.004 doi GBVA2015022000017.pica (DE-627)ELV024016535 (ELSEVIER)S0022-1759(15)30046-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Schmalenberg, Michael verfasserin aut Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Beaudoin, Christopher oth Bulst, Ludwig oth Steubl, Dominik oth Luppa, Peter B. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:427 year:2015 pages:36-41 extent:6 https://doi.org/10.1016/j.jim.2015.09.004 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 427 2015 36-41 6 045F 610 |
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10.1016/j.jim.2015.09.004 doi GBVA2015022000017.pica (DE-627)ELV024016535 (ELSEVIER)S0022-1759(15)30046-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Schmalenberg, Michael verfasserin aut Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Beaudoin, Christopher oth Bulst, Ludwig oth Steubl, Dominik oth Luppa, Peter B. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:427 year:2015 pages:36-41 extent:6 https://doi.org/10.1016/j.jim.2015.09.004 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 427 2015 36-41 6 045F 610 |
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10.1016/j.jim.2015.09.004 doi GBVA2015022000017.pica (DE-627)ELV024016535 (ELSEVIER)S0022-1759(15)30046-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Schmalenberg, Michael verfasserin aut Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Beaudoin, Christopher oth Bulst, Ludwig oth Steubl, Dominik oth Luppa, Peter B. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:427 year:2015 pages:36-41 extent:6 https://doi.org/10.1016/j.jim.2015.09.004 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 427 2015 36-41 6 045F 610 |
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10.1016/j.jim.2015.09.004 doi GBVA2015022000017.pica (DE-627)ELV024016535 (ELSEVIER)S0022-1759(15)30046-6 DE-627 ger DE-627 rakwb eng 610 610 DE-600 610 VZ 44.92 bkl Schmalenberg, Michael verfasserin aut Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care 2015transfer abstract 6 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. Beaudoin, Christopher oth Bulst, Ludwig oth Steubl, Dominik oth Luppa, Peter B. oth Enthalten in Elsevier Science Thompson, Jennifer A. ELSEVIER 969: Cesarean prevalence rates overtime by maternal characteristics 2018 JIM Amsterdam [u.a.] (DE-627)ELV001297813 volume:427 year:2015 pages:36-41 extent:6 https://doi.org/10.1016/j.jim.2015.09.004 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U SSG-OLC-PHA 44.92 Gynäkologie VZ AR 427 2015 36-41 6 045F 610 |
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Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care |
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Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. |
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Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. |
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Pneumonia is one of the leading causes of death worldwide. We present a magnetic bead fluorescent sandwich immunoassay that allows rapid serum measurement of the novel inflammation marker YKL40 (CHI3L1) at the point of care (POC) that could aid pneumonia diagnosis. The magnetic beads serve as the solid phase for separation of YKL40 from serum. The readout is performed using a small and robust fluorescence reader, which detects the turnover of a fluorescent substrate. The assay procedure, from sample addition to data retrieval, consists of three steps and is performed in less than 20min. The presented assay has a linear range from 3 to 111ng/mL, with a limit of detection of 2.9ng/mL. The average recoveries were found between 101 and 111%. The developed method was applied in sera from healthy subjects (n=14; c(YKL40)=50±49ng/mL) and from pneumonia patients (n=14; c(YKL40)=333.6±225ng/mL). The elevated YKL40 concentrations in pneumonia-diseased patients are in good agreement with previously published data. The POC-ready device provides a simple immunoassay that could help to optimize pneumonia inflammation diagnostics in low-resource settings. |
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Magnetic bead fluorescent immunoassay for the rapid detection of the novel inflammation marker YKL40 at the point-of-care |
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