Sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of Carcinoembryonic antigen
Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (G...
Ausführliche Beschreibung
Gespeichert in:
| Autor*in: |
Li, Yueyun [verfasserIn] |
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| Format: |
E-Artikel |
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| Sprache: |
Englisch |
| Erschienen: |
2017transfer abstract |
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| Umfang: |
9 |
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| Übergeordnetes Werk: |
Enthalten in: Enhancement with physicochemical and biological treatments in the removal of pharmaceutically active compounds during sewage sludge anaerobic digestion processes - Zhou, Haidong ELSEVIER, 2017, an international journal devoted to all aspects of electrode kinetics, interfacial structure, properties of electrolytes, colloid and biological electrochemistry, New York, NY [u.a.] |
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| Übergeordnetes Werk: |
volume:795 ; year:2017 ; day:15 ; month:06 ; pages:1-9 ; extent:9 |
| Links: |
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| DOI / URN: |
10.1016/j.jelechem.2017.04.042 |
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ELV02518332X |
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| 245 | 1 | 0 | |a Sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of Carcinoembryonic antigen |
| 264 | 1 | |c 2017transfer abstract | |
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| 520 | |a Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. | ||
| 520 | |a Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. | ||
| 700 | 1 | |a Zhang, Yihe |4 oth | |
| 700 | 1 | |a Li, Faying |4 oth | |
| 700 | 1 | |a Li, Mingdang |4 oth | |
| 700 | 1 | |a Chen, Lei |4 oth | |
| 700 | 1 | |a Dong, Yunhui |4 oth | |
| 700 | 1 | |a Wei, Qin |4 oth | |
| 773 | 0 | 8 | |i Enthalten in |n Elsevier |a Zhou, Haidong ELSEVIER |t Enhancement with physicochemical and biological treatments in the removal of pharmaceutically active compounds during sewage sludge anaerobic digestion processes |d 2017 |d an international journal devoted to all aspects of electrode kinetics, interfacial structure, properties of electrolytes, colloid and biological electrochemistry |g New York, NY [u.a.] |w (DE-627)ELV014792559 |
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10.1016/j.jelechem.2017.04.042 doi GBV00000000000093A.pica (DE-627)ELV02518332X (ELSEVIER)S1572-6657(17)30294-1 DE-627 ger DE-627 rakwb eng 540 620 540 DE-600 620 DE-600 660 VZ 660 VZ 530 600 670 VZ 51.00 bkl Li, Yueyun verfasserin aut Sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of Carcinoembryonic antigen 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Zhang, Yihe oth Li, Faying oth Li, Mingdang oth Chen, Lei oth Dong, Yunhui oth Wei, Qin oth Enthalten in Elsevier Zhou, Haidong ELSEVIER Enhancement with physicochemical and biological treatments in the removal of pharmaceutically active compounds during sewage sludge anaerobic digestion processes 2017 an international journal devoted to all aspects of electrode kinetics, interfacial structure, properties of electrolytes, colloid and biological electrochemistry New York, NY [u.a.] (DE-627)ELV014792559 volume:795 year:2017 day:15 month:06 pages:1-9 extent:9 https://doi.org/10.1016/j.jelechem.2017.04.042 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_22 GBV_ILN_40 GBV_ILN_105 51.00 Werkstoffkunde: Allgemeines VZ AR 795 2017 15 0615 1-9 9 045F 540 |
| spelling |
10.1016/j.jelechem.2017.04.042 doi GBV00000000000093A.pica (DE-627)ELV02518332X (ELSEVIER)S1572-6657(17)30294-1 DE-627 ger DE-627 rakwb eng 540 620 540 DE-600 620 DE-600 660 VZ 660 VZ 530 600 670 VZ 51.00 bkl Li, Yueyun verfasserin aut Sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of Carcinoembryonic antigen 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Zhang, Yihe oth Li, Faying oth Li, Mingdang oth Chen, Lei oth Dong, Yunhui oth Wei, Qin oth Enthalten in Elsevier Zhou, Haidong ELSEVIER Enhancement with physicochemical and biological treatments in the removal of pharmaceutically active compounds during sewage sludge anaerobic digestion processes 2017 an international journal devoted to all aspects of electrode kinetics, interfacial structure, properties of electrolytes, colloid and biological electrochemistry New York, NY [u.a.] (DE-627)ELV014792559 volume:795 year:2017 day:15 month:06 pages:1-9 extent:9 https://doi.org/10.1016/j.jelechem.2017.04.042 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_22 GBV_ILN_40 GBV_ILN_105 51.00 Werkstoffkunde: Allgemeines VZ AR 795 2017 15 0615 1-9 9 045F 540 |
| allfields_unstemmed |
10.1016/j.jelechem.2017.04.042 doi GBV00000000000093A.pica (DE-627)ELV02518332X (ELSEVIER)S1572-6657(17)30294-1 DE-627 ger DE-627 rakwb eng 540 620 540 DE-600 620 DE-600 660 VZ 660 VZ 530 600 670 VZ 51.00 bkl Li, Yueyun verfasserin aut Sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of Carcinoembryonic antigen 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Zhang, Yihe oth Li, Faying oth Li, Mingdang oth Chen, Lei oth Dong, Yunhui oth Wei, Qin oth Enthalten in Elsevier Zhou, Haidong ELSEVIER Enhancement with physicochemical and biological treatments in the removal of pharmaceutically active compounds during sewage sludge anaerobic digestion processes 2017 an international journal devoted to all aspects of electrode kinetics, interfacial structure, properties of electrolytes, colloid and biological electrochemistry New York, NY [u.a.] (DE-627)ELV014792559 volume:795 year:2017 day:15 month:06 pages:1-9 extent:9 https://doi.org/10.1016/j.jelechem.2017.04.042 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_22 GBV_ILN_40 GBV_ILN_105 51.00 Werkstoffkunde: Allgemeines VZ AR 795 2017 15 0615 1-9 9 045F 540 |
| allfieldsGer |
10.1016/j.jelechem.2017.04.042 doi GBV00000000000093A.pica (DE-627)ELV02518332X (ELSEVIER)S1572-6657(17)30294-1 DE-627 ger DE-627 rakwb eng 540 620 540 DE-600 620 DE-600 660 VZ 660 VZ 530 600 670 VZ 51.00 bkl Li, Yueyun verfasserin aut Sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of Carcinoembryonic antigen 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Zhang, Yihe oth Li, Faying oth Li, Mingdang oth Chen, Lei oth Dong, Yunhui oth Wei, Qin oth Enthalten in Elsevier Zhou, Haidong ELSEVIER Enhancement with physicochemical and biological treatments in the removal of pharmaceutically active compounds during sewage sludge anaerobic digestion processes 2017 an international journal devoted to all aspects of electrode kinetics, interfacial structure, properties of electrolytes, colloid and biological electrochemistry New York, NY [u.a.] (DE-627)ELV014792559 volume:795 year:2017 day:15 month:06 pages:1-9 extent:9 https://doi.org/10.1016/j.jelechem.2017.04.042 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_22 GBV_ILN_40 GBV_ILN_105 51.00 Werkstoffkunde: Allgemeines VZ AR 795 2017 15 0615 1-9 9 045F 540 |
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10.1016/j.jelechem.2017.04.042 doi GBV00000000000093A.pica (DE-627)ELV02518332X (ELSEVIER)S1572-6657(17)30294-1 DE-627 ger DE-627 rakwb eng 540 620 540 DE-600 620 DE-600 660 VZ 660 VZ 530 600 670 VZ 51.00 bkl Li, Yueyun verfasserin aut Sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of Carcinoembryonic antigen 2017transfer abstract 9 nicht spezifiziert zzz rdacontent nicht spezifiziert z rdamedia nicht spezifiziert zu rdacarrier Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. Zhang, Yihe oth Li, Faying oth Li, Mingdang oth Chen, Lei oth Dong, Yunhui oth Wei, Qin oth Enthalten in Elsevier Zhou, Haidong ELSEVIER Enhancement with physicochemical and biological treatments in the removal of pharmaceutically active compounds during sewage sludge anaerobic digestion processes 2017 an international journal devoted to all aspects of electrode kinetics, interfacial structure, properties of electrolytes, colloid and biological electrochemistry New York, NY [u.a.] (DE-627)ELV014792559 volume:795 year:2017 day:15 month:06 pages:1-9 extent:9 https://doi.org/10.1016/j.jelechem.2017.04.042 Volltext GBV_USEFLAG_U GBV_ELV SYSFLAG_U GBV_ILN_22 GBV_ILN_40 GBV_ILN_105 51.00 Werkstoffkunde: Allgemeines VZ AR 795 2017 15 0615 1-9 9 045F 540 |
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sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of carcinoembryonic antigen |
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Sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of Carcinoembryonic antigen |
| abstract |
Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. |
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Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. |
| abstract_unstemmed |
Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA. |
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Sandwich-type amperometric immunosensor using functionalized magnetic graphene loaded gold and silver core-shell nanocomposites for the detection of Carcinoembryonic antigen |
| url |
https://doi.org/10.1016/j.jelechem.2017.04.042 |
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true |
| author2 |
Zhang, Yihe Li, Faying Li, Mingdang Chen, Lei Dong, Yunhui Wei, Qin |
| author2Str |
Zhang, Yihe Li, Faying Li, Mingdang Chen, Lei Dong, Yunhui Wei, Qin |
| ppnlink |
ELV014792559 |
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z |
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oth oth oth oth oth oth |
| doi_str |
10.1016/j.jelechem.2017.04.042 |
| up_date |
2024-07-06T16:54:38.815Z |
| _version_ |
1803849437092511744 |
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In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA.</subfield></datafield><datafield tag="520" ind1=" " ind2=" "><subfield code="a">Herein, a novel and sensitive sandwich-type electrochemical immunosensor was fabricated for quantitative detection of carcinoembryonic antigen (CEA). In order to construct the base of the immunosensor, the gold nanoparticles (Au NPs) were immobilized on the surface of bare glassy carbon electrode (GCE) through electrochemical reduction of HAuCl4 solution. The electrodeposited gold nanoparticles (D-Au NPs) not only effective improve immobilization of primary anti-CEA antibody (Ab1) but also accelerate the electron transfer on the electrode interface due to the high specific surface area, good biocompatibility and superior electrical conductivity. Moreover, the amino functionalized magnetic graphene loaded gold and silver core-shell nanoparticles to adsorb nickel ion (GS-Fe3O4/AuAg/Ni2+), which were used as a novel label to load the secondary anti-CEA antibody (Ab2). The resultant nanocomposites possess high specific surface area, excellent electrochemical property, good biocompatibility and superior auxiliary catalytic activity due to the synergetic effect. The signal amplification strategy, using the synergetic effect present in Au@Ag/Fe3O4-GS/Ni2+ to improve immobilization of Ab2 and increase the reduction ability of the nanocomposites towards H2O2, improved the sensitivity of the immunosensor. Under the optimal conditions, a linear relationship between current signals and the concentrations of CEA was obtained in the range from 0.1pg/mL to 100ng/mL and the detection limit of CEA was 0.0697pg/mL (signal-to-noise ratio of 3). Furthermore, the as-proposed immunosensor showed excellent performance in detection of human serum samples. The results suggest that the proposed immunosensor will be promising in the diagnostics application for accurately quantitative detection of CEA.</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Zhang, Yihe</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Faying</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Li, Mingdang</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Chen, Lei</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Dong, Yunhui</subfield><subfield code="4">oth</subfield></datafield><datafield tag="700" ind1="1" ind2=" "><subfield code="a">Wei, Qin</subfield><subfield code="4">oth</subfield></datafield><datafield tag="773" ind1="0" ind2="8"><subfield code="i">Enthalten in</subfield><subfield code="n">Elsevier</subfield><subfield code="a">Zhou, Haidong ELSEVIER</subfield><subfield code="t">Enhancement with physicochemical and biological treatments in the removal of pharmaceutically active compounds during sewage sludge anaerobic digestion processes</subfield><subfield code="d">2017</subfield><subfield code="d">an international journal devoted to all aspects of electrode kinetics, interfacial structure, properties of electrolytes, colloid and biological electrochemistry</subfield><subfield code="g">New York, NY [u.a.]</subfield><subfield code="w">(DE-627)ELV014792559</subfield></datafield><datafield tag="773" ind1="1" ind2="8"><subfield code="g">volume:795</subfield><subfield code="g">year:2017</subfield><subfield code="g">day:15</subfield><subfield code="g">month:06</subfield><subfield code="g">pages:1-9</subfield><subfield code="g">extent:9</subfield></datafield><datafield tag="856" ind1="4" ind2="0"><subfield code="u">https://doi.org/10.1016/j.jelechem.2017.04.042</subfield><subfield code="3">Volltext</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_USEFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ELV</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">SYSFLAG_U</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_22</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_40</subfield></datafield><datafield tag="912" ind1=" " ind2=" "><subfield code="a">GBV_ILN_105</subfield></datafield><datafield tag="936" ind1="b" ind2="k"><subfield code="a">51.00</subfield><subfield code="j">Werkstoffkunde: Allgemeines</subfield><subfield code="q">VZ</subfield></datafield><datafield tag="951" ind1=" " ind2=" "><subfield code="a">AR</subfield></datafield><datafield tag="952" ind1=" " ind2=" "><subfield code="d">795</subfield><subfield code="j">2017</subfield><subfield code="b">15</subfield><subfield code="c">0615</subfield><subfield code="h">1-9</subfield><subfield code="g">9</subfield></datafield><datafield tag="953" ind1=" " ind2=" "><subfield code="2">045F</subfield><subfield code="a">540</subfield></datafield></record></collection>
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